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Rudi Kartika, Rudi
Jurusan Kimia Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Mulawarman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health

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UJI AKTIVITAS EKSTRAK ETANOL KULIT BATANG TUMBUHAN AFRIKA (Vernonia amygdalina Delile) TERHADAP PENURUNAN KADAR KOLESTEROL TOTAL PADA MENCIT JANTAN (Mus musculus) HIPERKOLESTEROLEMIA Miraz’hul Fahmi; Rudi Kartika; Hendra Priatna; Fathur Rahman; Muhammad Haissul Mahrus
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Blood cholesterol level which were higher than normal is a trigger of various diseases. The objective of the research was to know the anti-hypercholesterol effect of bark African plant. The research about utilization of ethanol extract of bark African plant (Vernonia amygdalina Delile) has been done, it proved could decrease total cholesterol levels in male mice (Mus musculus). In this research sample macerated with ethanol 96% and then concentrated by rotary evaporator, and gained 9,7% of yield. Based on the results of phytochemical test, secondary metabolites compound that contained in bark African plant are alkaloid, flavonoid, triterpenoid, steroid, phenolic and saponin. On antihiperkolesterolemia activity test by using male mice, there are several treatments that negative control (-), positive control (+) and three treatment with different doses i.e. a low doses (7.5 mg/kg BB), middle doses (15 mg/kg BB) and high doses (30 mg/kg BB). The parameter measured was blood serum of male mice by using a Cholesterol Test Strips. From this test it showed that the effectiveness of decreased total cholesterol level at middle doses of 15 mg/kg BB. Keywords: Bark African plant (Vernonia amygdalina Delile), Phytochemicals test, Total cholesterol levels, Cholesterol test strips
BIOSORPSI LOGAM BERAT Cr(VI) MENGGUNAKAN BAKTERI Streptococcus mutans Rida Awliyani; Rudi Kartika; Aman Sentosa Panggabean
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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The research about the biosorpsion of hexavalent chromium heavy metal [Cr(VI)] with Streptococcus mutans bacteria has been carried out. Biosorption has been done by exposure of chromium to broth culture, the consentration and optimum time happened on the 4 day with the biosorption percentage of Cr(VI) by Streptococcus mutans bacteria was obtained at a concentration of Cr(VI) 5 ppm, with a percentage of 90.80%. It shows Streptococcus mutans has potential as biosorbent of Cr(VI). Keywords: Biosorption, Cr(VI), Streptococcus mutans
PENYERAPAN LOGAM BERAT SECARA BIOKIMIAWI OLEH BAKTERI GRAM POSITIF DAN GRAM NEGATIF: A MINI RIVIEW Mahrus, Muhammad Haissul; Kartika, Rudi; Fahmi, Miraz’ul; Dimas P, Riansyah; Rahman, Fathur; Aswat, Raihan; Rizkirullah, M. Adhitya
PROSIDING SEMINAR KIMIA Vol 2 No 2 (2024): Prosiding SNKT III 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Dyes are one source of heavy metal waste originating from the textile industry, this is caused by the manufacture of dyes that use heavy metals as catalysts. Heavy metals are very dangerous because they cannot be broken down or degraded. Wastewater produced from the textile industry can contain dangerous metals, namely chromium (Cr), copper (Cu) and lead (Pb), where if these metals enter water bodies they can damage the food chain. Therefore, an alternative method is used that can reduce this waste, namely the biosorption method using microorganisms in the form of bacteria. This research uses gram positive bacteria in the form of Streptococcus mutans bacteria which will be exposed to chromium (VI) metal and gram negative bacteria in the form of Flavobacterium sp bacteria which will be exposed to lead (Pb) metal and Pseudomonas sp bacteria which will be exposed to chromium (VI) metal, lead. (Pb), and copper (Cu). The aim of this research was to determine the absorption ability of negative and gram-positive salt bacteria on these heavy metals. Literature results show that Streptococcus mutans bacteria are able to absorb chromium (VI) metal with a maximum of 90.80% with a concentration of 5 ppm on the 5th day. Flavobacterium sp bacteria are able to absorb lead metal (Pb) with a maximum of 96% with a concentration of 2 ppm on the 18th day, while Pseudomonas sp bacteria are able to absorb chromium (VI) metal with a maximum of up to 96.192% with a concentration of 5 ppm on the second day. -14, lead(Pb) metal can be absorbed to a maximum of 36.874% with a concentration of 4 ppm on the 16th day and Cu(II) metal can be absorbed to a maximum of 84.447% with a concentration of 3 ppm on the 16th day. Keywords: Biosorption, Bacteria, Metal
KAJIAN STATUS MUTU AIR SUNGAI MAHAKAM SEGMEN KABUPATEN MAHAKAM ULU BERDASARKAN METODE STORET Solman, Solman; Bonifasius, Bonifasius; Kartika, Rudi; Gelung, Antonius; Ricard, Romoldus Jonius; Ilang, Yerwanto
PROSIDING SEMINAR KIMIA Vol 2 No 2 (2024): Prosiding SNKT III 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Mahakam Ulu District is geographically located in the western part of East Kalimantan Province, where the Mahakam River flows. This upstream segment of the Mahakam River is the main river that functions as a transportation route, as raw water processed by Municipal Waterworks, used as a place for bathing, washing as well as serving as a lavatory by the community and a source of water for local community agriculture. Surface water sampling points consist of 14 stations, including ST I: Mahakam River (Long Pahangai Village), ST II: Melaseh River (Long Pahangai Village), ST III: Pahangai River (Long Pahangai Village), ST IV: Mahakam River (Tiong Ohang Village), ST V: Sebunut River (Long Bagun Village), ST VI: Alan River (Long Bagun Village), ST VII: Mahakam River (Ujoh Bilang Village), ST VIII: Melahan River (Long Melahan Village), ST IX: Mahakam River (Laham Village), ST X: Sungai Ratih (Damum Paroy village), ST XI: Mahakam River (Datah Bilang village), ST XII: Pari River (Datah Bilang village), ST XIII: Pataq River (Long Bagun sub-district) and ST XIV: Medang River (Long Bagun sub-district). Determination of water quality status using the STORET Method or Pollution Index Method. The STORET method is one of the commonly used methods for determining water quality status. With this STORET method can be known parameters that have met or exceeded water quality standards. From the results of the study, the water quality parameters that partially did not meet the class I quality standards were 6 parameters, among others: TSS, BOD, COD, DO and Faecal coliform and Total coliform, while for classes II, III and IV all parameters meet the quality standards according to regulations. Keywords: Activities around the river, river quality status, STORET Method
AMPLIFICATION OF pncA GENES FROM WATER BACTERIAL POST MINING IN SAMARINDA Suryadi, Ahmad; Astuti, Winni; Kartika, Rudi
PROSIDING SEMINAR KIMIA Vol 2 No 1 (2023): Prosiding SNK 2023
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Amplification of pncA genes from water bacterial post mining in Samarinda has been done. The aim of this research is to isolate chromosomal DNA and amplificated the pncA gene from water bacterial post mining in Samarinda. The research methode consist of chromosomal DNA isolation and amplification of pncA gene with polymerase chain reaction (PCR) technique. The result showed the size of chromosomal DNA is ± 10 kb. By using primer pncA gene, the result of amplification of the pncA gene showed that there were two sizes of amplicon, namely 0.7 kb and 0.2 kb. The conclusion that can be drawn was there is two sizes of amplicon pncA gene. Keywords: chromosomal DNA isolation, amplification of pncA gen, PCR
PHYTOCHEMICAL TEST AND STABILITY TEST OF PIGMENT OF KETAPANG LEAF (Terminalia catappa Linn.) Sulistiyawati, Rizki Ramadhani; Saleh, Chairul; Kartika, Rudi
JURNAL ATOMIK Vol 6 No 2 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

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Extraction of ketapang leaf (Terminalia catappa Linn) was carried out by maceration method using 96% ethanol. Phytochemical test results showed that the ketapang leaf extract contained tannin compounds. Stability test of ketapang leaf extract was carried out on the influence of temperature 40, 60 and 800C, the effect of pH (3, 4 and 5) and the effect of storage duration 1, 3 and 5 days using wavelength UV. The results of this study indicate that the ketapang leaf dye is stable at pH 3-4, stable decrease by heating at temperature 40-800C and stable at storage for 1-3 days.
CORRELATION OF Pb METAL ION LEVELS ON PROTEIN CONCENTRATION IN CRUSH CONSTRUCTION (Gafrarium tumidium) TAKEN IN THE COAST OF BONTANG, EAST KALIMANTAN Corebima, Damianus Develistur; Kartika, Rudi; Hindryawati, Noor
JURNAL ATOMIK Vol 5 No 2 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Research on the correlation of Pb metal ion levels to protein content in crush clams (Gafrarium tumidium) taken at the coast of the East Kalimantan Rhino Estuary has been conducted. This study aims to determine the levels of Pb metal ions and protein levels in blood clams based on the size and location of different samples. Correlation of Pb metal ion levels and protein in blood clams was tested using the Least Square method and Pearson product moment. Analysis of Pb metal ion levels using SSA (Atomic Absorption Spectrophotometer) and protein content analysis using the Kjeldahl method. In this study, the Pb metal ion level was obtained at the public port of Tanjung Limau, PPI Tanjung Limau and beach Lallo Sekangat with an average of 0.7474 mg/L, 0.5513 mg/L, and 0.2804 mg/L respectively. Crush clam protein content at Tanjung Limau, PPI Tanjung Limau and beach Lallo Sekangat with an average of 19.6544%, 14.6902%, and 10.9135% respectively. The correlation of Pb metal levels to protein levels in crush clams (Gafrarium tumidium) was stated positively with coefficient correlation results was strong (r = 0.7910).
ENDOPHYTIC BACTERIA SCREENING FROM THE LEAVES OF OKRA (Abelmoschus esculentus L. Moench) WHICH PRODUCED LIPASE Utami, Nanda Putri; Astuti, Winni; Kartika, Rudi
JURNAL ATOMIK Vol 5 No 2 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Endophytic bacteria found in plant tissues can produce enzymes, one of which is lipase. The purpose of this study was to screening endophytic bacteria from the leaves of Okra (Abelmoschus esculentus L. Moench) which produced lipases. The method used in this study is screening with media nutrient agar (NA) containing Rhodamine B and olive oil. The results showed that 4 of bacterial isolates (D1, D2, D3 and D4) from 14 endophytic bacterial isolates had lipase activity. The crude lipase extract was quantitatively tested for lipase activity by the titrimetry method. The highest lipase activity was shown by isolates with a D1 code of 1.50 U/mL.
PEMBUATAN ETANOL DARI UMBI TALAS (Colocasia esculenta [L] Schott) DENGAN PENAMBAHAN GELATIN SEBAGAI SUMBER NITROGEN Saccharomyces Cerevisiae Abimanyu, Priem Reyhan; Kartika, Rudi; Sitorus, Saibun
JURNAL ATOMIK Vol 4 No 2 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Ethanol have made from taro’s flour with an addition of gelatin as nutrition source for Saccharomyces cereviciae. Ethanol are made within process hydrolysis, fermentation, distillation and Gas Chromatography analysis. Starch hydrolysis process are enzymatic through liquification and saccharification process with an addition of alpha-amylase and gluco-amylase which conversed starch into glucose. Fermentation process are used time variation within 5, 6, 7 and 8 days and gelatin as nutrition source are used in concentration to 0,5 %, 1 % and 1,5 % (v/v). The highest ethanol concentration are obtained in addition of 1 % gelatin with 7 days of fermentation which are 41,648 %.
SELEKSI BAKTERI POTENSIAL PENGHASIL ENZIM HIDROLITIK DARI SAMPEL TANAH TEMPAT PEMBUANGAN AKHIR BUKIT PINANG SAMARINDA Admaja, Junior Try; Astuti, Winni; Kartika, Rudi
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1401

Abstract

Bacteria are one of the microorganisms that have the potential to produce hydrolytic enzymes. This research was carried out to isolate bacteria from the soil of the Bukit Pinang Samarinda Final Disposal Site and select bacteria that produce cellulase, protease and lipase enzymes. Bacteria from landfill soil were isolated using the spread plate method and 10 single colony bacterial isolates were selected which were then coded TPA 1 – TPA 10. The results of the selection of bacteria producing hydrolase enzymes showed that 9 isolates were capable of producing protease enzymes, 6 isolates were capable of producing cellulase enzymes and 4 isolates were capable of producing cellulase enzymes. The isolate was able to produce the lipase enzyme.
Co-Authors Abimanyu, Priem Reyhan Adha, Laila Sufina Aditya, Roy Admaja, Junior Try Adnyana, Ketut Cahyadi Ahmad Suryadi, Ahmad Alimuddin - Alimuddin - Aman S Panggabean Aman S Panggabean, Aman S Aman S. Panggabean Aman Sentosa Panggabean Andi Kurniawan Annuary Laksono Arifwan - Ary Rizki Darmawi Ary Rizki Darmawi, Ary Rizki Aswad, Muhammad Raihan Aswat, Muhammad Raihan Aswat, Raihan Azka, Andi Aulia Urrahman Bayu Iskandar Bohari Yusuf Bohari Yusuf Bonifasius, Bonifasius Bustanussalam Bustanussalam Bustanussalam Bustanussalam, Bustanussalam Chairul Saleh Chairul Saleh Corebima, Damianus Develistur Delina Khairunnisa Dilo Jhosea Luciano Eduardo Dimas P, Riansyah Djihan Ryn Pratiwi, Djihan Ryn Dwi Lestari Dwi Susanto Edi Novian Jaya Edman Tandirerung Eka Damayanti Setyningrum Eka Setia Rini Eka Siswanto Syamsul Eno Julia Shafarini Erwin - Erwin Erwin Eva Marliana, Eva Fahmi, Miraz’hul Fahmi, Miraz’ul Farida Farida Fathur Rahman Fathur Rahman fatur Rahman, fatur Fauzi Rachman Fauzi Rachman Firmansyah - Fitriana, Mila Gelung, Antonius Hamsiana - Hendra Priatna Hendric Surya Putra I Wayan Arief Pradana Putra Iskandar, Bayu Jaya, Edi Novian Jofri, Dirga Hosea Seventeen Jofri, Dirga Hosea Seventin Julianto, Faisal Reja Kalmud Nia Tekha Laksono, Annuary Lila Karina Nuryanti Lilik Sulastri Lumban Gaol Veronica Destiana Mahrus, Muhammad Haissul Maria Amelia Kristianingrum Marliani, Nelly Marlina, Eva Mila Fitriana Mira Wahyuni Mira Wahyuni, Mira Miraz’hul Fahmi Muhammad Adhitya Rizkirullah Muhammad Haissul Mahrus Muhammad Harry Rahmadan Muhammad Raihan Aswat Muhammad Sigit Ariski Nasution, Safridah Hannum Noor Hindryawati Noor Hindryawati, Noor Nur Asmawati Nur, Istianti Irfa'a Octavianus, Riyan Octavianus, Riyan Partomouan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Prastiwi, Mela Pratama, Riansyah Dimas Rahmat Gunawan Rahmat Gunawan Rahmat Gunawan Rezky Estrada Ricard, Romoldus Jonius Rico Ramadhan, Rico Rida Awliyani Ripaldy Apriansya Riyan Octavianus Rizkirullah, M. Adhitya Rizkirullah, Muhammad Adhitya Rizkirullah, Muhammad Aditya Rizkullah, Muhammad Adhitya rizky maulida amalia hanif Ruga, Ritbey Rutdianti Rutdianti Rutdianti, Rutdianti Sahriani, Andi Saibun Sitorus Sanian, Sanian Sari Setianingsih Setianingsih, Sari Setyningrum, Eka Damayanti Shafarini, Eno Julia Shaparuddin Nur Arifullah Simanjuntak, Partomuan Solman, Solman Suaibatul Aslamiah Subur P Pasaribu Sudrajat Sudrajat Sukanadi, I Putu Sukma Wiratama Sulistiani, Oktaviana Dewi Sulistiyawati, Rizki Ramadhani Syelvia Lousiana Kalauw Syelvia Lousiana Ningsih Kalauw Tandirerung, Edman Tanjung, Faisal Trisnawati, Eka Elly Ulfa, Yulyanti Sartika Utami, Nanda Putri Wahyu Budi Utama Wahyu Budi Utama Wahyu Teguh Santoso Wahyu Teguh Santoso Wajdie, Farid Winni Astuti Winni Astuti, Winni Wulan Verani Yeni Agustina Yeni Agustina, Yeni Yerwanto Ilang Yerwanto Ilang, Yerwanto