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Rudi Kartika, Rudi
Jurusan Kimia Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Mulawarman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health

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BIOPRODUKSI SENYAWA KIMIA ANTIDIABETES OLEH ISOLAT KAPANG ENDOFIT KUMIS KUCING (Orthosiphon spicatus B. B. S.) Sulastri, Lilik; Kartika, Rudi; Simanjuntak, Partomuan
PROSIDING SEMINAR KIMIA Vol 3 No 1 (2024): Prosiding SNK 2024
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Kumis kucing plant (Orthosiphon spicatus B. B. S.) are one of the plants that have been used as diuretic, antidiabetic and antihypertensive drugs. Identification of chemical compounds, and antidiabetic activity assay of bioproduction of endophytic fungi with code B.Os.1F that isolated from the stems of kumis kucing have been carried out. Two fermentation methods (dynamic and static fermentation) to determine the pH. The tlc profile analysis and pH showed that B.Os.1F isolate by dynamic fermentation for 12 days had the potential to produce chemical compounds as antidiabetics at pH 4 ~ 6. The product of fermentation are extracted with ethyl acetate, then fractionated by column chromatography (SiO2): (i) n-hexane-ethyl acetate [10:1 ~ 1:1]; (ii) chloroform-methanol (20:1 ~ 1:1) based on “bioassay guided fractionation” with a-glucosidase enzyme inhibition assay. The isolate of B.Os.1F.2-3 had an activity of 76 %, and identify based on UV-Vis, Fourier-Transform Infra Red (FT-IR) spectroscopies and gas chromatography-mass spectrometer (GC-MS) are estimated to be Ergosta-5,7,9(11),22-tetraen-3-ol compounds. Keywords : Endophytic fungus, Orthosiphon spicatus B.B.S., α-glucosidase enzyme, ergosta-5,7,9(11),22-tetraen-3-ol.
POTENSI BEBERAPA SUMBER KARBOHIDRAT SEBAGAI BAHAN DASAR PEMBUATAN BIOETANOL MELALUI FERMENTASI Rahman, Fathur; Mahrus, Muhammad Haissul; Fahmi, Miraz’hul; Pratama, Riansyah Dimas; Aswat, Muhammad Raihan; Rizkirullah, Muhammad Aditya; Kartika, Rudi
PROSIDING SEMINAR KIMIA Vol 3 No 1 (2024): Prosiding SNK 2024
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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This literature review aims to determine the potential of various carbohydrate sources as raw materials for bioethanol production through fermentation to achieve maximum ethanol concentration. The hydrolysis process is conducted enzymatically using microbes during the liquefaction stage and gluco-amylase during the saccharification stage, followed by fermentation by microbes. The literature results show that the carbohydrate source, Ganyong tuber (Canna edulis Kerr.), produces a maximum ethanol concentration of 84.451% with a fermentation period of 7 days. Keywords: Bioethanol, Fermentation, Hydrolysis, Carbohydrate
ANALISIS KADAR LOGAM Pb, Mn dan KANDUNGAN PROTEIN PADA DAGING UDANG WINDU (Penaeus sp.) YANG DIAMBIL DIPERAIRAN SUNGAI DONDANG KECAMATAN MUARA JAWA Octavianus, Riyan; Kartika, Rudi; Hindryawati, Noor
JURNAL ATOMIK Vol 3 No 1 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Research on metal concentration of Pb, Mn and protein content in windu shrimp’s meat (Penaeus sp.) analysis in Dondang river, Muara Jawa have been done. This research is aimed to determine the concentration of heavy metal Pb, Mn and concentration of protein inside windu shrimp based by size and different sampling location. Concentration of heavy metal Pb, Mn and protein are correlated using Least Square method. Concentration of heavy metal Pb and Mn are analyzed by using AAS (Atomic Absorption Spectrophotometry) and concentration of protein are analyzed by using Kjeldahl method. Result of this research is the concentration heavy metal Pb and Mn in sampling point 1 in average concentration are 0.556 mg/L and 0.463 mg/L, sampling point 2 are 0.790 mg/L and 0.738 mg/L, sampling point 3 are 0.876 mg/L and 0.905 mg/L. Concentration of protein in windu shrimp in sampling point 1 in average are 23.4928%, sampling point 2 are 28.1701% and sampling point 3 are 29.1622%. Correlation of concentration of heavy metal Pb and Mn to the concentration of protein in windu shrimp (penaeus sp.) with R = 0.791; r = 0.948.
ETHANOL PRODUCTION FROM TUBER GANYONG (Canna Edulis Kerr.) BY Saccharomyces cerevisiae WITH THE ADDITION OF Spirulina Sp. Sukanadi, I Putu; Kartika, Rudi; Hindryawati, Noor
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

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This study demonstates the potential of starch tuber ganyong (Canna edulis Kerr.) as a substance for making ethanol by Saccharomyces cerevisiae with microalgae addition of Spirulina sp. as a source of nutrients in the fermentation process of ethanol has been carried out. The study also aims to determine the amount of nutrient concentration and fermentation time was used to generate the optimum ethanol content. Hydrolysis process by enzymatic is done through a liquification phase with α-amylase and saccharification phase with gluko-amylase. Furthermore the process of fermentation stage with the variation of the time (132 hours, 156 hours, 180 hours and 204 hours) using the yeast Saccharomyces cerevisiae with the addition of Spirulina sp. as a nutrient in the variation of Spirulina sp. concentrations (0,6 % (w/v), 1,2 % (w/v) and 1,8 % (w/v)). The result showed the sugar reducing after of hydrolysis was obtained with amount of 0,527 %, increased from the initial state with amount of 0,351 %. The highest ethanol content be found on the increase nutrient with amount of 1,2 % (w/v) by is time of fermentation to180 hours.
POTENTIALITY OF METHANOL EXTRACT OF SALAM LEAVES (Syzygium polyanthum) TO INHIBIT THE GROWTH OF Staphylococcus aureus AND Salmonella typhi Trisnawati, Eka Elly; Astuti, Winni; Kartika, Rudi
JURNAL ATOMIK Vol 5 No 1 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Uji Fitokimia dan aktivitas antibakteri ekstrak metanol daun salam (Syzygium polyanthum) telah dilakukan. Penelitian ini bertujuan untuk mengetahui metabolit sekunder yang terkandung pada ekstrak metanol daun salam dan konsentrasi hambat minimum (KHM) ekstrak tersebut. Uji aktivitas antibakteri dilakukan dengan metode difusi menggunakan kertas cakram. Ekstrak metanol daun salam (Syzygium polyanthum) metanol daun salam mengandung steroid, flavonoid dan fenolik. Konsentrasi hambat minimum ekstrak metanol daun salam (Syzygium polyanthum) terhadap Staphylococcus aureus sebesar 0-1% dan terhadap Salmonella typhi sebasar 1-2%.
THE OPTIMUM PRODUCTION TIME OF LIPASE FROM ENDOPHYTIC BACTERIA LEAVES OF Macaranga hullettii King ex Hook.f. Marliani, Nelly; Astuti, Winni; Kartika, Rudi
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Endophyte bacteria isolate from the leaves of Macaranga hullettii King ex Hook.f. has potential to producer lipase. In the study was conducted to filter lipase producing endophyte bacteria from Macaranga hullettii King ex Hook.f. and determine the optimum production time. The results of screening endophyte bacteria showed 6 isolates of endophyte bacteria that had the potential to produce lipase and 1 isolate was chosen which is number 5 to determine the optimum production time. The optimum production time of lipase from Macaranga hullettii King ex Hook.f. leaves for 72 hours with activity that is 2.92 U/mL.
ETHANOL PRODUCTION FROM SUWEG TUBER (Amorphophallus camapanulatus BI.) BY Saccharomyces cerevisiae WITH THE ADDITION OF NPK NUTRITION Adnyana, Ketut Cahyadi; Kartika, Rudi; Sitorus, Saibun
JURNAL ATOMIK Vol 5 No 1 (2020)
Publisher : Jurusan Kimia FMIPA UNMUL

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Research of ethanol made from suweg tuber flour (Amorphophallus camapanulatus BI.) with the addition of NPK as nutrition Saccharomyces cerevisiae, has been done. The ethanol making process consists of hydrolysis, fermentation, distillation, and testing carried out by Gas Chromatography. The hydrolysis stage is carried out by adding α-amylase and glucosyl amylase in order to break the polysaccharide chain into monosaccharide. The fermentation stage is carried out with time variation and NPK concentration. It can be seen that the addition of nutrients of 3.70 g (2%) NPK with a 7 day fermentation time resulted in the most optimum ethanol concentration of 67.357% and an ethanol volume of 7 mL.
ISOLATION OF ENDOPHYTIC BACTERIA ON GINSENG JAWA LEAVES (Talinum triangulare (Jacq.) Willd) Nur, Istianti Irfa'a; Astuti, Winni; Kartika, Rudi
JURNAL ATOMIK Vol 6 No 1 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

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Endophytic bacteria are bacteria that live in plant tissues. These bacteria produce extracellular enzymes, one of which is the lipase. This research was conducted to isolate the endophytic bacteria of Ginseng Jawa (Talinum triangulare (Jacq.) Willd) leaves which produced lipase and lipase screening from these bacteria. Isolation was carried out on nutrient agar (NA) media and screening of endophytic bacteria were carried out on nutrient agar (NA) media containing olive oil and Rhodamin B. Isolation results obtained by 5 single colonies and one colony of positive endophytic bacteria that produces lipase which characterized by the presence of orange-colored luminescence on the surface of the bacterial colony
ETHANOL PRODUCTION FROM SAGO FERMENTATION USING Saccharomyces cerevisiae AND GELATIN AS A SOURCE OF NUTRIENT FOR MICROBA -, Firmansyah; Sitorus, Saibun; Kartika, Rudi
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Ethanol from sago flour through fermentation using Saccharomyces cerevisiae and gelatin as nutrition for microbe have been done. The making of ethanol through processes of hydrolysis, fermentation, distillation and analysis of ethanol concentration using Gas Chromatography instrument. The hydrolysis process of sago flour are used two steps which is liquification and saccharification using alpha-amylase and glucoamylase which will convert the starch into glucose. The fermentation process of glucose are done with time variation 5, 6, 7 and 8 days to the concentration variation of gelatin addition which is 0,5 %, 1 % and 1,5 % (w/v) as nutrition source for microbe. The obtained ethanol from fermentation process are purified through distillation. Furthermore, analysis of ethanol concentration are used Gas Chromatography. The analysis results are showed the highest ethanol concentration obtained in 7 days with gelatin addition 1,5 % are 79,50 %.
ANTIHYPERURISEMIA ACTIVITY TEST OF ETHANOL EXTRACT FROM LEAVES OF KLUWIH (Artocarpus altilis (Parkinson) Fosberg) AGAINST MALE MICE (Mus musculus) Wajdie, Farid; Kartika, Rudi; Saleh, Chairul
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Phytochemical screening on ethanol extract from leaves of kluwih (Artocarpus altilis (Parkinson) Fosberg) has been carried out and that results showed the presence of flavonoids, alkaloids, phenolic, triterpenoids and steroids. The ethanol extract from leaves of kluwih (Artocarpus altilis (Parkinson) Fosberg) was tested for its antihyperuricemic experimentally in the laboratory with doses of 28 mg/kg, 56 mg/kg and 112 mg/kg which were done orally in male mice (Mus musculus) for the purpose of antihyperuricemic assess the effect of the decrease uric acid in male mice. Uric acid measurements carried out on days 0, 15, 17, 19, 21, 23, 25 and 27 by using uric acid strip and uric acid meter. Treatments with dosage variation kluwih leaves extract (Artocarpus altilis (Parkinson) Fosberg) shows that a dose of 112 mg/kg give blood uric acid levels decrease by 64.23%. The results of this study showed a decrease in uric acid levels as a result of ethanol extract from leaves of kluwih (Artocarpus altilis (Parkinson) Fosberg).
Co-Authors Abimanyu, Priem Reyhan Adha, Laila Sufina Aditya, Roy Admaja, Junior Try Adnyana, Ketut Cahyadi Ahmad Suryadi, Ahmad Alimuddin - Alimuddin - Aman S Panggabean Aman S Panggabean, Aman S Aman S. Panggabean Aman Sentosa Panggabean Andi Kurniawan Annuary Laksono Arifwan - Ary Rizki Darmawi Ary Rizki Darmawi, Ary Rizki Aswad, Muhammad Raihan Aswat, Muhammad Raihan Aswat, Raihan Azka, Andi Aulia Urrahman Bayu Iskandar Bohari Yusuf Bohari Yusuf Bonifasius, Bonifasius Bustanussalam Bustanussalam Bustanussalam Bustanussalam, Bustanussalam Chairul Saleh Chairul Saleh Corebima, Damianus Develistur Delina Khairunnisa Dilo Jhosea Luciano Eduardo Dimas P, Riansyah Djihan Ryn Pratiwi, Djihan Ryn Dwi Lestari Dwi Susanto Edi Novian Jaya Edman Tandirerung Eka Damayanti Setyningrum Eka Setia Rini Eka Siswanto Syamsul Eno Julia Shafarini Erwin - Erwin Erwin Eva Marliana, Eva Fahmi, Miraz’hul Fahmi, Miraz’ul Farida Farida Fathur Rahman Fathur Rahman fatur Rahman, fatur Fauzi Rachman Fauzi Rachman Firmansyah - Fitriana, Mila Gelung, Antonius Hamsiana - Hendra Priatna Hendric Surya Putra I Wayan Arief Pradana Putra Iskandar, Bayu Jaya, Edi Novian Jofri, Dirga Hosea Seventeen Jofri, Dirga Hosea Seventin Julianto, Faisal Reja Kalmud Nia Tekha Laksono, Annuary Lila Karina Nuryanti Lilik Sulastri Lumban Gaol Veronica Destiana Mahrus, Muhammad Haissul Maria Amelia Kristianingrum Marliani, Nelly Marlina, Eva Mila Fitriana Mira Wahyuni Mira Wahyuni, Mira Miraz’hul Fahmi Muhammad Adhitya Rizkirullah Muhammad Haissul Mahrus Muhammad Harry Rahmadan Muhammad Raihan Aswat Muhammad Sigit Ariski Nasution, Safridah Hannum Noor Hindryawati Noor Hindryawati, Noor Nur Asmawati Nur, Istianti Irfa'a Octavianus, Riyan Octavianus, Riyan Partomouan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Partomuan Simanjuntak Prastiwi, Mela Pratama, Riansyah Dimas Rahmat Gunawan Rahmat Gunawan Rahmat Gunawan Rezky Estrada Ricard, Romoldus Jonius Rico Ramadhan, Rico Rida Awliyani Ripaldy Apriansya Riyan Octavianus Rizkirullah, M. Adhitya Rizkirullah, Muhammad Adhitya Rizkirullah, Muhammad Aditya Rizkullah, Muhammad Adhitya rizky maulida amalia hanif Ruga, Ritbey Rutdianti Rutdianti Rutdianti, Rutdianti Sahriani, Andi Saibun Sitorus Sanian, Sanian Sari Setianingsih Setianingsih, Sari Setyningrum, Eka Damayanti Shafarini, Eno Julia Shaparuddin Nur Arifullah Simanjuntak, Partomuan Solman, Solman Suaibatul Aslamiah Subur P Pasaribu Sudrajat Sudrajat Sukanadi, I Putu Sukma Wiratama Sulistiani, Oktaviana Dewi Sulistiyawati, Rizki Ramadhani Syelvia Lousiana Kalauw Syelvia Lousiana Ningsih Kalauw Tandirerung, Edman Tanjung, Faisal Trisnawati, Eka Elly Ulfa, Yulyanti Sartika Utami, Nanda Putri Wahyu Budi Utama Wahyu Budi Utama Wahyu Teguh Santoso Wahyu Teguh Santoso Wajdie, Farid Winni Astuti Winni Astuti, Winni Wulan Verani Yeni Agustina Yeni Agustina, Yeni Yerwanto Ilang Yerwanto Ilang, Yerwanto