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Effects of Cadmium Exposure on Lipid Peroxidation and Chlorinative Stress of Rat Kidney Suhartono, Eko; Triawanti, Triawanti; Leksono, Amin Setyo
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (780.729 KB) | DOI: 10.21776/ub.jels.2015.005.01.01

Abstract

In addition to a wide range of adverse effects on human health, toxic metals such as cadmium (Cd) can promote kidney damage. In this study, we proposed the toxicological properties of these metals which are partly related to lipid peroxidation and chlorinative stress. Thus our study aimed to measure the lipid peroxidation and chlorinative stress in kidney of rats exposed to Cd. The study was performed on 32 male rats (Rattus novergicus) weighing 300±10 grams with normal activity. Rats were acclimatized for a week before the treatment. The animals were divided into two major groups with one group for control (K0) and the other groups were exposed to 3 mg/L CdSO4 through the drinking water. The animals in each groups were divided again into two small groups with different time of exposure. The time of exposure are subacute (six weeks) and subchronic (eight weeks). After the exposure the kidney from all treated animals showed the significant increase of MDA and AOPP levels. In conclusion, subacute and subchronic exposure of Cd caused the kidney damage through lipid peroxidation and chlorinative stress, as shown by increasing of MDA and AOPP levels. Keywords: AOPP, Cadmium, Chlorinative Stress, Lipid Peroxidation, MDA
Lethal Concentration 50 (LC50 - 96 hours) Nile Tilapia (Oreochromis niloticus) exposed Cypermethrin-based Pesticide Yuniari, Shinta Hiflina; Hertika, Asus Maizar Suryanto; Leksono, Amin Setyo
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (917.984 KB) | DOI: 10.21776/ub.jels.2016.006.02.01

Abstract

In aquaculture of an irrigation, the use of cypermethrin-based pesticide can harm nile tilapia which cultured within and its impact on humans who consume the fish. Thus it is necessary to study to determine the threshold of this cypermethrin-based pesticide. Determining the thresholds was using the test of LC50 - 96 hours (Lethal Concentration 50-96 hour) which tested tilapia that dead by 50%, which can be used as a benchmark threshold for cypermethrin-based pesticide. Test of LC50 - 96 hours consist of preliminary test and advanced test. Lethal Concentration 50 (LC50 - 96 hours) of cypermethrin-based pesticide towards nile tilapia (Oreochromis niloticus) is 0.082 ppm. At these doses, tilapia death reached 50%. This concentration is expressed as a threshold usage of cypermethrin-based pesticide on the environment around the aquaculture of tilapia. Keywords: Cypermehrin-based pesticide, LC5096 hour, Nile tilapia.
Antifeedant Effect of Moringa oleifera (L.) Leaf and Seed Extract on Growth and Feeding Activity of Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) Tridiptasari, Amelia; Leksono, Amin Setyo; Siswanto, Dian
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.918 KB) | DOI: 10.21776/ub.jels.2019.009.01.05

Abstract

This study was conducted to evaluate the effect of Moringa oleifera leaf and seed extract on length, weight, and feeding activity of Spodoptera litura. Leaf and seed were extracted by maceration method in 70% ethanol. Bioassays were conducted by using five concentrations (0%, 5%, 10%, 15%, 20%) on 5 larvae by leaf-dip method with 5 times replication. The result showed that leaf and seed of Moringa extract contained alkaloid, flavonoid, and saponin which gave antifeedant effect to growth and feeding activity of larvae. The increased extract concentrations are lead to weight loss and the length shrinkage of larvae. The extracts promoted prolongation of the larval instar. Reduction of food intake of the larva is in line with the concentration increase of extracts. The antifeedant effect also affects time to stop feeding and give mortality of larvae.Keywords: antifeedant, leaf, moringa, seed, S. litura
Screening potential local seed species for hydroseeding of post-coal mining land multilayering revegetation Anshari, Muhammad Fadhil; Fernandes, Adji Achmad Rinaldo; Leksono, Amin Setyo; Arisoesilaningsih, Endang
Journal of Degraded and Mining Lands Management Vol. 11 No. 1 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2023.111.4969

Abstract

This study aimed to screen some potential local seed grains for hydroseeding and describe their characteristics based on the literature review and a year of hydroseeding application. This study used six species/variants of Poaceae (Coix lacryma-jobi, Eleusine indica, Setaria italica (brown, black, and red), Sorghum timorense, S. bicolor, Themeda arundinaceae), five species of Leguminosae (Adenanthera pavonina, Cajanus cajan, Sesbania grandiflora, S. sesban, Indigofera sp.), a species of Cyperaceae (Cyperus javanicus), Sapindaceae (Sapindus rarak), Rhamnaceae (Ziziphus jujuba), and Moringaceae (Moringa oleifera). A seed germination test was held using soil media placed in 5 pots per species until 15 days after sowing (DAS). Characters were scored, and data were statistically analyzed. A field record of one-year hydroseeding applied on 6 m x 6 m post-coal mining land plot was presented. Some data such as pH H2O, pH KCl, conductivity, and soil organic carbon among hydroseeding areas, unrevegetated areas, and reference sites were observed. Results showed that there were 13 of 17 species could variably germinate. The fastest germination time was recorded for S. timorense, S. bicolor, red S. italica, C. cajan, and S. grandiflora, while the highest germination rate (≥50%) was black S. italica (80%), brown S. italica (58%) and S. bicolor (50%). The annual black and brown S. italica, S. bicolor, and S. timorense were highly recommended to be used in hydroseeding. The perennial C. cajan, Indigofera sp., S. sesban, and T. arundinaceae were also potential to be added into a hydroseeding slurry to improve pioneer vegetation multilayering structure and diversity.
Health education strategies to improve handwashing and waste disposal in Klakah Village Rizki, Lailina Aminatur; Leksono, Amin Setyo; Batoro, Jati
Jurnal Pembangunan dan Alam Lestari Vol. 17 No. 2 (2026): Jurnal Pembangunan dan Alam Lestari
Publisher : Graduate School of Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpal.2026.017.02.06

Abstract

Sanitation challenges, including low compliance with handwashing practices and improper waste disposal, remain critical public health concerns in Klakah Village. This study aimed to evaluate the effectiveness of integrated health education strategies using both print and digital media, and to examine their association with handwashing and waste disposal behaviors. A descriptive-analytic study with a cross-sectional design was conducted involving 84 households selected through stratified random sampling. The intervention consisted of health education delivered through posters, leaflets, digital presentations, and short videos to enhance community awareness and behavioral change. Data were collected through structured observations, interviews, and questionnaires, and analyzed using bivariate statistical tests to assess the relationships between educational exposure, attitudes, habits, and sanitation-related behaviors. The results revealed a statistically significant yet weak positive association between attitudes, habitual practices, and sanitation behaviors. Households exposed to combined print and digital educational media demonstrated more favorable attitudes toward handwashing and waste management, as well as improved behavioral practices compared to baseline conditions. Despite the modest strength of the associations, the findings suggest that multi-modal health education strategies can contribute to incremental improvements in sanitation behavior at the household level. This study underscores the importance of integrating traditional and digital communication approaches to enhance the effectiveness of public health interventions, particularly in rural settings with limited sanitation infrastructure. Strengthening community engagement and ensuring sustained educational efforts are essential to achieving long-term behavioral change and improving environmental health outcomes.
Co-Authors Abdul Hakim Abida, Sakinah Hilya Abu Bakar Sambah, Abu Bakar Agung Sih Kurnianto Akhmad Rizali Akram, Andi Muhammad Aminuddin Afandhi Aminudin Afandhi Anisa Zairina Anshari, Muhammad Fadhil Apriliani , Adini ardhanys wari putri Arief Rachmansyah Asus Maizar Suryanto H Azzamuddin, Haidar Bagyo Yanuwiadi Bagyo Yanuwiyadi, Bagyo Bambang Semedi Bedjo Bedjo Bernadeta Putri Irma Dalia Budi Purwantiningsih Catur Retnaningdyah Dian Siswanto Eko Suhartono Endang Arisoesilaningsih ervin jumiatin Fajarwati, Prisca Kiki Fajarwati, Santi Kusuma Feriwibisono, Bambang Fernandes, Adji Achmad Rinaldo fevilia suksma wardani Frank Leonardo Apituley Hafid Zain Muttaqien, Hafid Zain Hamidi, Faris Harsuko Riniwati Hasan Zayadi Herawati Herawati Hiasinta Guruh H. iin nursaidah Irfan Mustafa Jati Batoro Jr sulthan ardillah Juhari, Anita Kartikasari, Retna Khatimah, Ainul Luchman Hakim M. Fathoni madaniatul islamiyah Mandhalika, Vianta Marsoedi Marsoedi Maulana, Fujianor Millah, Najmatul Moch Sasmito Djati Muhammad Yunus Nanang Johanudin Nobukazu Nakagoshi Nurwijayanti Parmawati, Rita Pramana, Ida Idewa Agung Willy Purnomo Purnomo Putra, Heru Wiwoho Supadi Putri, Ninda Merisa Qurotaayuina, Riska Putri Rahmah, S. Fatiyatur Redy Alviantono Risman Risman Rizki, Lailina Aminatur Rodiyati Azrianingsih Rodliyati Azrianingsih Rony Irawanto Sagista, Reza Setyadin, Yuris Shinta Hiflina Yuniari Slamet Wahyudi Soemarno Soemarno Suheni Suheni Sunoko, Rudy Susanto, Muhamad Azmi Dwi Tria Irma Muhibah, Tria Irma Triawanti Triawanti Tridiptasari, Amelia Turhadi Turhadi Yunita Fera Rahmawati Zaenal, Kusuma Zulfaidah Penata Gama