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UJI EFEKTIVITAS EKSTRAK KULIT BATANG POHON KAYU ULIN (Eusideroxylon zwageri) TERHADAP Aggregatibacter actinomycetemcomitans Fidya Mariam; I Wayan Arya Krishnawan Firdaus; Fransiska Uli Artha Panjaitan
Dentin Vol 4, No 2 (2020)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Aggressive periodontitis is a disease that had very quickly rate of development and damage. One of the causes of this disease is the presence of Aggregatibacter actinomycetemcomitans. The effective antibiotic to handle the growth of A.actinomycetemcomitans in the aggressive periodontitis treatment is metronidazole gel 25%. Ulin stem bark which contains phenolic, flavonoid, tannin, alkaloid, terpenoid and saponin so that it can be basic ingredients for alternative medicine. Purpose: To analyze the inhibitory and killing levels of ulin stem bark extract against the growth of A.actinomycetemcomitans. Materials and methods: True experimental research with post test only control group design, the number of treatments were 9 with 4 replications, which came from 5%, 10%, 20%, 40%, 60%, 80%, 100% concentrations, Metronidazole gel 25 %, and sterile aquades against A.actinomycetemcomitans with a total sample were 36 samples. Result: One Way ANOVA and Post-Hoc Gomes Howell tests showed the average absorbance’s reduction has significant differences, Kruskall Wallis and Post-Hoc Mann Whitney tests showed the number of colonies with significant differences. Conclusion: The minimum inhibitory concentration of ulin stem bark extract was at 5% concentration. Minimum bactericidal concentration was at 20% concentration which has an antibacterial effectiveness equal to Metronidazole gel 25%. Keywords: Aggregatibacter actinomycetemcomitans, MBC, MIC, Ulin Stem Bark Extract ABSTRAKLatar Belakang: Periodontitis agresif merupakan penyakit yang laju perkembangan dan kerusakan terjadi sangat cepat. Salah satu penyebab penyakit ini adalah bakteri Aggregatibacter actinomycetemcomitans. Salah satu antibiotik yang efektif dalam menangani pertumbuhan bakteri A.actinomycetemcomitans adalah Metronidazole gel 25%. Kulit batang pohon kayu ulin yang mengandung fenolik, flavonoid, tannin, alkaloid, terpenoid dan saponin sehingga dapat menjadi bahan dasar alternatif obat. Tujuan: Menganalisis kadar hambat dan bunuh ekstrak kulit batang pohon kayu ulin terhadap pertumbuhan bakteri A.actinomycetemcomitans Metode: Penelitian eksperimental murni dengan rancangan penelitian post test only, jumlah perlakuan adalah 9 dengan 4 kali pengulangan, yang berasal dari konsentrasi 5%, 10%, 20%, 40%, 60%, 80%, 100%, Metronidazole gel 25%, dan akuades steril terhadap A.actinomycetemcomitans dengan total sampel adalah 36 sampel. Hasil: Uji One Way ANOVA dan Post-Hoc Gomes Howell menunjukan bahwa selisih rata rata absorbansi memiliki perbedaan yang signifikan, uji Kruskall Wallis dan Post-Hoc Mann Whitney menunjukan jumlah koloni dengan perbedaan yang signifikan. Kesimpulan: Nilai kadar hambat minimum pada ekstrak kulit batang pohon kayu ulin terdapat pada konsentrasi 5%. Kadar bunuh minimum terdapat pada konsentrasi 20% yang mempunyai efektivitas antibakteri yang setara dengan Metronidazole gel 25%. Kata kunci : Aggregatibacter actinomycetemcomitans, Ekstrak Kulit Batang Pohon Ulin, KBM, KHM

UJI TOKSISITAS EKSTRAK KULIT BATANG ULIN (Eusideroxylon zwageri) TERHADAP SEL FIBROBLAS BHK-21 SECARA IN VITRO Ismi Natasya Salwa; I Wayan Arya Krishnawan Firdaus; Aulia Azizah
Dentin Vol 5, No 3 (2021)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Antioxidants can come from plants that contain flavonoids such as ironwood. The bark of ironwood is declared effective to be used as a mouthwash because of the content of flavonoids, phenolics, tannins, saponins, alkaloids, and terpenoids. Ironwood bark extract to be used as an alternative material must be safe for oral tissues, so it is necessary to do a toxicity test. Objective: To analyze the toxic effect of ironwood bark extract through IC50 on BHK-21 fibroblast cells. Methods: This study was a pure laboratory experimental study with posttest-only control group design, consisting of 12 groups with 10 treatment groups given ironwood bark extract at concentrations of 5%, 15%, 25%, 35%, 45%, 55%. , 65%, 75%, 85%, and 95% and 2 control groups, namely cell control and media control. It was repeated 3 times so that the total sample was 36 samples. Toxicity test media used MTT assay which produced color absorbance and cell viability was calculated. Results: The results showed that the cell viability of the entire treatment group was >60% so it had no toxic effect. In addition, based on the IC50> 0.1%, which is 3.746%, it has no toxic effect on BHK-21 fibroblast cells. Conclusion: Ironwood bark extract at concentrations of 5%, 15%, 25%, 35%, 45%, 55%, 65%, 75%, 85%, and 95% had no toxic effect on BHK-21 fibroblast cells. Keywords: BHK-21 fibroblast cells, Ironwood bark extract, , toxicity ABSTRAKLatar Belakang: Antioksidan dapat berasal dari tanaman yang mengandung flavonoid seperti ulin. Kulit batang ulin dinyatakan efektif untuk dijadikan obat kumur karena adanya kandungan flavonoid, fenolik, tanin, saponin, alkaloid, dan terpenoid. Ekstrak kulit batang ulin untuk dijadikan sebagai bahan alternatif harus bersifat aman untuk jaringan rongga mulut sehingga perlu dilakukan uji toksisitas. Tujuan: Menganalisis efek toksik ekstrak kulit batang ulin melalui IC50 terhadap sel fibroblas BHK-21. Metode: Penelitian eksperimental laboratoris murni dengan posttest-only with control group design, terdiri dari 12 kelompok dengan 10 kelompok perlakuan yang diberi ekstrak kulit batang ulin konsentrasi 5%, 15%, 25%, 35%, 45%, 55%, 65%, 75%, 85%, dan 95% dan 2 kelompok kontrol, yaitu kontrol sel dan kontrol media. Dilakukan 3 kali pengulangan sehingga total sampel berjumlah 36 sampel. Media uji toksisitas menggunakan MTT assay yang menghasilkan absorbansi warna dan dilakukan perhitungan viabilitas sel. Hasil: Viabilitas sel seluruh kelompok perlakuan adalah >60% sehingga tidak memiliki efek toksik. Selain itu, berdasarkan nilai IC50>0,1% yaitu sebesar 3,746% tidak memiliki efek toksik terhadap sel fibroblas BHK-21. Kesimpulan: Ekstrak kulit batang ulin pada konsentrasi 5%, 15%, 25%, 35%, 45%, 55%, 65%, 75%, 85%, dan 95% tidak memiliki efek toksik terhadap sel fibroblas BHK-21. Kata kunci: Esktrak kulit batang ulin, sel fibroblas BHK-21, toksisitas.

UJI FITOKIMIA KUALITATIF DAN KUANTITATIF EKSTRAK KULIT BUAH RAMBAI (Baccaurea Motleyana) KONSENTRASI 100% Diza Afira Hutasuhut; Didit Aspriyanto; I Wayan Arya Krishnawan Firdaus
Dentin Vol 6, No 2 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v6i2.6394

ABSRACTBackground: A typical plant from Kalimantan that is effective as a herbal medicine is rambai fruit (Baccaurea Motleyana). The rind of the rambai fruit contains secondary metabolites, namely alkaloids, phenolics, and flavonoids which has activity as an antioxidant, anti-inflammatory, anti-inflammatory, and antimicrobial. Utilization of rambai fruit peel (Baccaurea Motleyana) in addition to the potential to be used as an alternative herbal medicine can also reduce waste from rambai fruit skin. Therefore, quantitative and qualitative phytochemical tests are needed to obtain information on the content of each compound in the rambai fruit peel. Objective: To analyze the results of qualitative and quantitative phytochemical tests of rambai fruit peel extract (Baccaurea Motleyana) with a concentration of 100%. Methods: Non-experimental with qualitative laboratory examination to determine the compound and quantitative to determine the concentration of the sample. Results: The results of qualitative and quantitative phytochemical tests showed that alkaloid compounds were 136.41 mg/ml, saponins were 102.35 mg/ml, phenolic compounds were 109.96 mg/ml, flavonoids were 71.33 mg/ml, triterpenoids were 353, 47 mg/ml, and steroids at 28.71 mg/ml. Conclusion: The highest levels were found in triterpenoids at 353.47 mg/ml and the lowest levels were found in steroid compounds at 28.71 mg/ml.Keywords: Baccaurea Motleyana, Qualitative test, Quantitative test. ABSTRAKLatar Belakang: Tanaman khas dari Kalimantan yang efektif sebagai obat herbal adalah buah rambai (Baccaurea motleyana). Kulit buah rambai memiliki kandungan metabolit sekunder yaitu alkaloid, fenolik, dan flavonoid yang memiliki aktivitas sebagai antioksidan, anti radang, antiinflamasi, dan antimikroba. Pemanfaatan kulit buah rambai (Baccaurea motleyana) selain berpotensi dapat dijadikan alternatif obat herbal dapat juga mengurangi limbah dari kulit buah rambai. Oleh sebab itu, diperlukan uji fitokimia kuantitatif dan kualitatif untuk mendapatkan informasi kandungan tiap senyawa pada kulit buah rambai. Tujuan: Menganalisis hasil uji fitokimia kualitatif dan kuantitatif ekstrak kulit buah rambai (Baccaurea motleyana) konsentrasi 100%. Metode: Non eksperimental dengan pemeriksaan laboratorium secara kualitatif untuk menentukkan senyawa dan kuantitatif untuk menetapkan kadar sampel. Hasil: Hasil uji fitokimia kualitatif dan kuantitatif didapatkan senyawa alkaloid sebesar 136,41 mg/ml, saponin sebesar 102,35 mg/ml, fenolik sebesar 109,96 mg/ml, flavonoid sebesar 71,33 mg/ml, triterpenoid sebesar 353,47 mg/ml, dan steroid sebesar 28,71 mg/ml. Kesimpulan: Kadar tertinggi terdapat pada senyawa triterpenoid sebesar 353,47 mg/ml dan kadar terendah terdapat pada senyawa steroid sebesar 28,71 mg/ml. Kata kunci: Baccaurea Motleyana, Uji Kualitatif, Uji Kuantitatif.

COMPARISON OF KELAKAI AND KATUK EXTRACT COMBINATION TO 0.2% CHLORHEXIDINE GLUCONATE AGAINST Porphyromonas gingivalis Erisa Lidiya; I Wayan Arya Krishnawan Firdaus; Fransiska Uli Arta Panjaitan
Dentin Vol 7, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i2.9722

Background: Periodontal disease is the second most frequent oral disease in Indonesia, which includes periodontitis. Porphyromonas gingivalis is the predominant bacteria in chronic periodontitis. This disease is usually treated with 0.2% chlorhexidine gluconate mouthwash. However, 0.2% chlorhexidine gluconate has several adverse effects, such as tooth discoloration, irritation of the oral mucosa, and changes in taste sensation. Therefore, an herbal-based mouthwash is needed. A combination of kelakai leaf and katuk leaf extract contains antibacterial substances such as flavonoid, tannin, steroid, alkaloid, and saponin, which can be used as an alternative therapy for chronic periodontitis. Purpose: To compare the inhibitory zone of kelakai and katuk extract combination with 0.2 Chlorhexidine gluconate against Porphyromonas gingivalis. Method: True experimental design with 13 treatment groups and 3 repetitions for each group. The treatment groups include combinations of kelakai leaf extract with 25%, 50%, 75%, 100% concentrations and katuk leaf extract with 20%, 40%, 80% concentrations and 0.2% chlorhexidine gluconate, which were tested on Porphyromonas gingivalis with a total of 39 samples. The inhibitory zone is measured with caliper after incubated for 48 hours. Results: One-Way ANOVA revealed significant differences between all treatment groups and 0.2% chlorhexidine gluconate. Consequently, the Bonferroni post-hoc test was performed, which showed that the antibacterial activity of 75% kelakai leaf and 80% katuk leaf extract did not differ significantly from the activity of 0.2% chlorhexidine gluconate. Conclusion: The combination of 75% kelakai leaf and 80% katuk leaf extract has an equal antibacterial activity to 0.2% chlorhexidine gluconate against Porphyromonas gingivalis statistically. Keywords : 0.2 % Chlorhexidine gluconate, chronic periodontitis, combination of kelakai and katuk leaf extract, inhibitory activity, Porphyromonas gingivalis

COMPARISON OF THE INHIBITORY EFFECT BETWEEN ULIN (Eusideroxylon zwageri) BARK EXTRACT AND Chlorhexidine gluconate 0.2% AGAINST Streptococcus sanguinis Marthia Eka Nurdila; I Wayan Arya Krishnawan Firdaus; Raden Harry Dharmawan Setyawardhana
Dentin Vol 7, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i2.9717

Background: Gingivitis is caused by a build up of plaque bacteria, one of which is Streptococcus sanguinis. Streptococcus sanguinis act as an anchor for the attachment of oral organisms that colonize the surface of the teeth, forming plaque so that it becomes the development of gingivitis. Currently, many natural ingredients, as alternatives, have antibacterial effects. One of which is Eusideroxylon zwageri, because it contains flavonoids, tannins, phenolics, saponins, alkaloids, and terpenoids. Purpose: to compare the inhibitory effect between ulin bark extract and Chlorhexidine gluconate 0.2% against Streptococcus sanguinis. Method: True experimental research with post test only with control group design using ulin bark extract concentrations of 20%, 40%, 60%, 80%, and 100% and Chlorhexidine gluconate 0.2% as the K(+). The maceration method was used to extract ulin bark while the inhibitory test was using the diffusion method with 6 treatment groups and 4 replications, so that there were 24 samples. All treatment groups were incubated 24 hours at 37oC then measurement of inhibition zones was using callipers. Results: The results of the test showed that ulin bark extract concentrations of 20%, 40%, 60%, 80%, 100%, and Chlorhexidine gluconate 0.2% obtained an average inhibition zone with diameter of 7.18 mm, 9.06 mm, 11.08 mm, 13 mm, 15.09 mm, and 18.14 mm. Analysis of One Way ANOVA and Post Hoc Games-Howell data showed a significant difference between treatment groups. Conclusion: Ulin bark extract can inhibit the growth of Streptococcus sanguinis but has not been able to equal to Chlorhexidine gluconate 0.2%. Keywords: Chlorhexidine gluconate 0,2%, gingivitis, inhibitory activity, ulin bark extract, Streptococcus sanguinis.

PENGARUH EKSTRAK DAUN KARAMUNTING (Rhodomytrus tomentosa) TERHADAP KADAR TROMBOSIT SETELAH PAPARAN SINAR-X RADIOGRAFI PERIAPIKAL Ahda Ahda Annisa; Didit Aspriyanto; Nurdiana Dewi; I Wayan Arya Krishnawan Firdaus; Debby Saputera
Dentin Vol 7, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i3.10748

Latar Belakang : Paparan radiasi sinar-X radiografi periapikal dapat merusak sel darah salah satunya yaitu trombosit dengan cara membentuk radikal bebas sehingga dapat terjadinya penurunan pada sel darah. Kandungan oleh ekstrak daun karamunting dapat menangkal radikal bebas dan meningkatkan sel darah dari paparan radiasi sinar-X radiografi periapikal. Senyawa metabolit sekunder pada daun karamunting memiliki tiga kandungan senyawa tertinggi yaitu flavonoid, phenol dan tanin. Tujuan: Menganalisis pengaruh ekstrak daun karamunting terhadap kadar trombosit mencit jantan setelah paparan sinar-X radiografi periapikal. Metode : Penelitian ini menggunakan metode true experimental dengan rancangan post test only with control group design menggunakan 30 ekor mencit dibagi menjadi 5 kelompok. Kelompok K- adalah diberikan ekstrak daun karamunting tetapi tidak diberikan paparan radiasi sinar-X, kelompok K+ adalah diberikan radiasi sinar-X radiografi periapikal tanpa diberikan ekstrak daun karamunting, kelompok P1 adalah diberikan ekstrak daun karamunting dan paparan radiasi sinar-X radiografi periapikal pengulangan 1 kali, kelompok P2 adalah diberikan ekstrak daun karamunting dan paparan radiasi sinar-X radiografi periapikal pengulangan 7 kali dan kelompok P3 adalah diberikan ekstrak daun karamunting dan paparan radiasi sinar-X radiografi periapikal pengulangan 10 kali. Hasil : Hasil uji Post Hoc Bonferoni menunjukan perbedaan bermakna antar kelompok kontrol dan kelompok perlakuan. Kesimpulan : Terdapat pengaruh kadar trombosit pada mencit jantan (Mus musculus) pemberian ekstrak daun karamunting setelah paparan sinar-X radiografi periapikal. Kata kunci : Ekstrak daun karamunting, Radiografi periapikal, trombosit

UJI TOKSISITAS KAPSUL EKSTRAK IKAN TOMAN (Channa micropeltes) TERHADAP PERUBAHAN PROFIL DARAH TIKUS WISTAR Muhammad Ikhlasul Amal Sangadji; Amy Nindia Carabelly; Maharani Laillyza Apriasari; I Wayan Arya Krishnawan Firdaus
Dentin Vol 9, No 2 (2025)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v9i2.17745

ABSTACTBackground: The people of South Kalimantan traditionally use Haruan fish as an alternative medicine to accelerate wound healing. However, Haruan fish is difficult to cultivate, making Toman fish a potential alternative. Toman fish contains 5.35% albumin, whereas Haruan fish contains 4.35% albumin. Objective: To determine whether Toman fish extract capsules exert toxic effects on hematological profiles. Methods: This study consisted of three treatment groups: a group receiving Toman fish extract capsules at a dose of 0.7 grams, a positive control group receiving Haruan fish extract capsules at a dose of 0.7 grams, and a negative control group receiving only BR2 feed. The treatments were administered twice daily for 28 days. For administration, the capsule shells were opened, and 500 grams of dried extract were mixed with distilled water and given to Wistar rats using an oral gavage. After 28 days, blood samples were collected from the rats and analyzed using a Sysmax hematology analyzer. Results: The mean hemoglobin levels in the treatment, positive control, and negative control groups were 11.8 g/dl, 12.02 g/dl, and 12.12 g/dl, respectively. The mean erythrocyte counts in the treatment, positive control, and negative control groups were 6.46 million/mm³, 7.07 million/mm³, and 5.50 million/mm³, respectively. The mean leukocyte counts in the treatment, positive control, and negative control groups were 17.86 million/mm³, 13.62 million/mm³, and 9.24 million/mm³, respectively. Conclusion: Administration of Toman fish extract capsules at a dose of 0.7 grams produced negative effects on erythrocyte and leukocyte levels, while hemoglobin levels were not affected. These findings indicate that Toman fish extract capsules exhibit toxic effects on hematological profiles.Keywords: haruan fish, hematological profile, toman fish, toxicity test ABSTRAK Latar Belakang: Masyarakat Kalimantan Selatan menggunakan ikan Haruan sebagai obat alternatif mempercepat penyembuhan luka. Akan tetapi ikan Haruan sulit dibudidayakan sehingga dipilih ikan Toman untuk alternatif. Ikan Toman mengandung albumin 5,35%, sedangkan ikan Haruan mengandung albumin 4,35%. Tujuan : Untuk mengetahui apakah kapsul ekstrak ikan Toman memiliki efek toksik terhadap profil hematologi. Metode : Penelitian ini meliputi tiga kelompok perlakuan yaitu pemberian kapsul ekstrak ikan Toman dosis 0,7 gram, kontrol positif berupa kapsul ekstrak ikan Haruan dosis 0,7 gram serta kontrol negatif yang hanya diberikan pakan BR2. Perlakuan dilakukan sebanyak 2 kali sehari selama 28 hari. Cara perlakuan adalah dengan membuka cangkang kapsul, kemudian ekstrak kering 500 gram ditambahkan aquades dan diberikan ke tikus Wistar menggunakan sonde lambung. Setelah 28 hari, tikus diambil sampel darah dan diuji menggunakan alat Hematology analyzer sysmax. Hasil : Rata-rata jumlah hemoglobin pada kelompok perlakuan, kelompok kontrol positif, dan kelompok kontrol negatif masing-masing adalah 11,8 g/dl, 12,02 g/dl, dan 12,12 g/dl. Rata-rata dari jumlah eritrosit pada kelompok perlakuan, kelompok kontrol positif, dan kelompok kontrol negatif masing-masing adalah 6,46 juta/mm3, 7,07 juta/mm3, dan 5,50 juta/mm3. Rata-rata dari jumlah leukosit pada kelompok perlakuan, kelompok kontrol positif, dan kelompok kontrol negatif masing-masing adalah 17,86 juta/mm3, 13,62 juta/mm3, dan 9,24 juta/mm3. Kesimpulan : Terdapat pengaruh negatif pemberian kapsul ekstrak ikan Toman dosis 0,7 gram terhadap perubahan kadar eritrosit dan leukosit, sedangkan pada hemoglobin tidak terdapat pengaruh. Hal ini menunjukkan bahwa kapsul ekstrak ikan Toman memiliki efek toksik terhadap profil darah.Kata Kunci : ikan haruan, ikan toman, uji toksisitas, profil hematologi

PERBANDINGAN EFEKTIVITAS MEDIA POP-UP BOOK & VIDEO ANIMASI KARTUN TERHADAP PENGETAHUAN KESEHATAN GIGI DAN MULUT PADA ANAK SD (Tinjauan Siswa SDN Kuripan 2 Banjarmasin) Stevani Stevani; Raden Harry Dharmawan Setyawardhana; Beta Widya Oktiani; I Wayan Arya Krishnawan Firdaus
Dentin Vol 8, No 3 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i3.14229

Background: Dental and oral health is part of overall body health. In Indonesia, dental and oral health problems are still relatively high and the proportion of correct dental and oral health knowledge among elementary school children is still low. Providing health education through pop up books and animated cartoon videos can be used as information media to increase knowledge of dental and oral health in elementary school children. Objective: This study aims to analyze dental and oral health knowledge in elementary school children through pop up book media and animated cartoon videos on dental and oral health knowledge in elementary school children at SDN-SN Kuripan 2 Banjarmasin, Banjar Regency. Methods: Using a true experimental design with a pretest-posttest with control design with a simple random sampling technique. The research was carried out at SDN-SN Kuripan 2 Banjarmasin, Banjar Regency with a sample of 76 respondents. Results: The results of the T-Test Normality test data analysis showed that there was no difference in dental and oral health knowledge among elementary school children and before and after counseling (pop up book: p=13.16; animated cartoon video: p=13.63), There was no difference in dental and oral health knowledge in elementary school children between pretest and posttest (pop up book: p= 13.16; animated cartoon video: p=13.63). The results of the Mann Whitney test data analysis were p=0.097, which means oh, it is accepted, indicating there is no difference in knowledge of dental and oral health among elementary school children. Conclusion:. The conclusion of this research is that health knowledge education through pop up books and animated cartoon videos is equally effective for dental and oral health knowledge in elementary school children.Keywords: Counseling, dental and oral health, Pop Up Book, animated cartoon video ABSTRAKLatar belakang: Kesehatan gigi dan mulut merupakan bagian dari kesehatan tubuh secara keseluruhan. Di Indonesia masalah kesehatan gigi dan mulut masih tergolong tinggi dan proporsi pengetahuan kesehatan gigi dan mulut pada anak sekolah dasar yang benar masih rendah. Pemberian penyuluhan kesehatan melalui pop up book dan video animasi kartun dapat dijadikan sebagai media informasi untuk meningkatkan pengetahuan Kesehatan gigi dan mulut pada anak sekolah dasar. Tujuan: Penelitian ini bertujuan untuk menganalisis pengetahuan kesehatan gigi dan mulut pada anak sekolah dasar melalui media pop up book dan video animasi kartun terhadap pengetahuan kesehatan gigi dan mulut pada anak sekolah dasar pada siswa SDN-SN Kuripan 2 Banjarmasin, Kabupaten Banjar. Metode: Menggunakan true eksperimental design dengan rancangan pretest-postest with control design dengan teknik pengambilan sampel simple random sampling. Penelitian dilaksanakan di SDN-SN Kuripan 2 Banjarmasin, Kabupaten Banjar dengan sampel berjumlah 76 responden. Hasil: Hasil analisis data uji Normalitas T-Test menunjukkan tidak ada perbedaan pengetahuan Kesehatan gigi dan mulut pada anak sekolah dasar dan sebelum dan setelah penyuluhan (pop up book: p=13,16; video animasi kartun: p=13,63), tidak ada perbedaan pengetahuan kesehatan gigi dan mulut pada anak sekolah dasar antara pretest dan posttest (pop up book: p= 13,16; video animasi kartun: p=13,63). Hasil analisis data uji Mann Whitney p=0,097 yang berarti oh diterima menunjukkan tidak ada perbedaan pengetahuan kesehatan gigi dan mulut pada anak sekolah dasar Kesimpulan:. Kesimpulan penelitian ini adalah penyuluhan pengetahuan kesehatan melalui pop up book dan video animasi kartun sama efektif untuk pengetahuan kesehatan gigi dan mulut pada anak sekolah dasar.

UJI TOKSISITAS EKSTRAK DAUN KARAMUNTING (Rhodomyrtus tomentosa (Aiton) Hassk.) TERHADAP GINJAL TIKUS WISTAR (Berdasarkan Ureum dan Kreatinin) M. Ridhotama Wibowo; Beta Widya Oktiani; Melisa Budipramana; Maharani Laillyza Apriasari; I Wayan Arya Krishnawan Firdaus
Dentin Vol 8, No 2 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i2.13113

ABSTRACTBackground: Karamunting leaf have been used among community as traditional medication. Karamunting leaf have many properties because they contain secondary metabolite compounds such as flavonoids, triterpenoids, phenols, saponins and tannins. Administration as medicine is usually through oral. Oral administration of karamunting leaf in high dosage is considered to damage kidney microscopically. In vivo toxicity testing can be done to determine the toxicity effects of caramunting leaf extract at doses 600, 1200, 2400 mg/kg body weight before being tested on humans. Purpose: This study was conducted to determine whether karamunting leaf extract is toxic to the kidneys of Wistar rats subchronically with the parameters ureum and creatinine. Methods: The Rhodomyrtus Tomentosa (Aiton) Hassk. leaf were extracted using 96% ethanol and then given to male Wistar strain (Rattus norvegicus) with a 600, 1200, and 2400 mg/kg/body weight two times a day for 28 days. Rat blood was taken to check the levels of urea and creatinine. Result: The kidney ureum levels of Wistar rats in all treatment groups were still normal and did not exceed the normal range of ureum (10-50 mg/dL) while creatinine levels in all treatment groups were potentially toxic because they exceeded normal limits (0.578-1.128 mg/dL). Conclusion: The parameters of ureum and creatinine levels are not toxic because both can reduce the average value of both levels although some decrease significantly and some do not.Keywords : Creatinine, Excretion, Karamunting leaf, Kidney, Toxicity, Ureum ABSTRAKLatar Belakang: Daun karamunting telah digunakan oleh masyarakat sebagai obat tradisional. Daun karamunting banyak memiliki khasiat karena memiliki kandungan senyawa metabolit sekunder seperti flavonoid, triterpenoid, fenol, saponin dan tanin. Pemberiannya sebagai obat biasanya melalui oral. Pemberian daun ini secara oral dengan dosis tinggi diduga dapat merusak ginjal secara mikroskopis. Pengujian toksisitas secara in vivo dapat dilakukan untuk mengetahui efek toksisitas ekstrak daun karamunting pada dosis 600, 1200, 2400 mg/kg BB sebelum diujikan pada manusia. Tujuan: Penelitian ini dilakukan untuk mengetahui apakah ekstrak daun karamunting bersifat toksik terhadap ginjal tikus Wistar secara subkronik dengan parameter ureum dan kreatinin. Metode: Daun Rhodomyrtus Tomentosa (Aiton) Hassk. diekstraksi menggunakan etanol 96% dan kemudian diberikan pada tikus jantan galur Wistar (Rattus norvegicus) dengan dosis 600, 1200, dan 2400 mg/kg BB dua kali sehari selama 28 hari. Darah tikus diambil untuk memeriksa kadar urea dan kreatinin. Hasil: Kadar ureum ginjal tikus Wistar pada semua kelompok perlakuan masih normal dan tidak melebihi kisaran normal ureum (10-50 mg/dL), dan kadar kreatinin pada semua kelompok perlakuan juga normal karena tidak melebihi batas normal (0,578-1,128 mg/dL). Kesimpulan: Parameter kadar ureum dan kreatinin tidak toksik karena keduanya dapat menurunkan nilai rata-rata dari kedua kadar walaupun ada yang turun secara signifikan dan ada yang tidak. Kata kunci: Ekskresi, Ekstrak Daun Karamunting, Ginjal, Kreatinin, Toksisitas, Ureum

Lactobacillus sp. IDENTIFICATION IN CARIES-AFFECTED STUDENTS IN SMP NEGERI 1 SUNGAI PINANG KABUPATEN BANJAR Rosihan Adhani; Isnur Hatta; Muhammad Genadi Askandar; I Wayan Arya Krishnawan Firdaus
Dentin Vol 9, No 3 (2025)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v9i3.17915

ABSTRAKLatar Belakang: Karies adalah penyakit infeksi yang menyerang jaringan keras gigi. Karies adalah penyakit multifaktorial, yang dipicu oleh interaksi antara inang, mikroorganisme/agen, substrat, dan waktu. Salah satu agen patogen yang paling utama adalah Lactobacillus sp. Agen ini sebagian besar ditemukan pada lesi karies aktif, dapat menghasilkan asam laktat dan merupakan organisme yang toleran terhadap lingkungan asam. Paparan timbal juga merupakan faktor yang dapat memperburuk karies. Timbal adalah antagonis kalsium dan dapat menghambat metabolisme kalsium dalam remineralisasi gigi. Timbal dapat mengendap ke dalam air minum setelah mengkristal di udara, dibantu oleh hujan. Paparan ini sangat mengkhawatirkan bagi masyarakat yang masih bergantung pada air sungai yang tidak disaring untuk memenuhi kebutuhan sehari-hari mereka. Tujuan: Penelitian ini bertujuan untuk mengidentifikasi koloni Lactobacillus sp. pada anak-anak yang terkena karies dan menilai perbedaan antara anak-anak yang mengonsumsi air sungai dan anak-anak yang mengonsumsi air sumur. Metode: Penelitian ini merupakan studi analitik observasional dengan desain kelompok kontrol menggunakan pendekatan potong lintang, yang dilakukan di SMP Negeri 1 Sungai Pinang Kabupaten Banjar, November-Desember 2017. Populasi penelitian terdiri dari 60 siswa, yang dikategorikan menjadi dua kelompok (masing-masing 30 siswa): kelompok pengguna air sungai dan kelompok pengguna air sumur. Indeks DMF-T dan sampel usap gigi dicatat untuk dianalisis. Sampel usap kemudian dikirim ke laboratorium untuk diolah dengan pewarnaan dan menggunakan metode Huccer untuk menilai koloni Lactobacillus sp. Hasil: Hasil penelitian menunjukkan bahwa terdapat perbedaan signifikan antara jumlah koloni Lactobacillus sp. dan indeks DMT pada anak-anak pengguna air sungai dan anak-anak pengguna air sumur.Keywords: indeks DMF-T, karies, konsumsi air, timbal ABSTRACTBackground: Caries is an infectious disease affecting a tooth’s hard tissues. It is a multifactorial disease, initiated by the interaction of host, microorganisms/agents, substrates, and time. One of the most pathogenic agents is Lactobacillus sp. This agent is mostly found in active lesions of caries, can produce lactate acid and is an acidic-environment tolerant organism. Lead exposure is also a factor that can worsen caries. Lead is a calcium-antagonist and can hamper the metabolism of calcium in remineralization of tooth. Lead can precipitate into drinking water after being crystallized in the air, assisted by rain. This exposure is especially worrying to the community which still depends on unfiltered water from the river to fulfill their daily needs. Purpose: This study was proposed to identify Lactobacillus sp. colony in caries-affected children and assess the difference between river water consuming and well-water consuming children. Methods: This was an observational analytics with control group design study using cross sectional approach, performed in SMP Negeri 1 Sungai Pinang Kabupaten Banjar, November-December 2017. The population was 60 students, categorized into two groups (30 students each): river water consuming and well-water consuming groups. The samples’ DMF-T indexes and teeth swab samples were recorded for analysis. The swab samples were then delivered to laboratories to be treated by staining and using Huccer methods to assess the Lactobacillus sp. colony. Results: The results showed that there was a significant difference between colony count of Lactobacillus sp. and DMT-index in both river water consuming and well-water consuming children. Keywords: caries, DMF-T index, lead, water consumption

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