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Integrated Metabolomic and Functional Assessment of Sexed Frozen Semen in Holstein Friesian Bulls Yusuf, M.; Diansyah, A. M.; Sahiruddin, S.; Masturi, M.; Maulana, T.; Said, S.; Rahmat, R.; Alfian, A. M.; Adam, A. A. S.; Yusri, A. N. H. S.; Nurlatifah, A.; Amrullah, M. F.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.19

Abstract

This study examined the differences in sperm quality and metabolite profiles among X and Y sperm in Friesian Holstein bulls. It also tried to find specific metabolites that can help improve the accuracy of sexed semen use in humid tropical dairy farms. Semen samples from five bulls were separated by sex and tested for movement, live sperm count, shape, and membrane and acrosome health. The tests used Computer-Assisted Sperm Analysis (CASA) and regular microscope checks. Metabolite analysis was performed using LC-HRMS, followed by various statistical tests, pathway checks, and ROC curve analysis. The outcomes showed that X sperm had better movement and acrosome health, while Y sperm had stronger membranes, fewer shape problems, and moved faster. The untargeted metabolite study showed clear differences between the two groups. X sperm had more identified metabolites and greater average levels. The crucial metabolites found more in X sperm were D-(−)-fructose, L-(+)-lactic acid, and sn-glycero-3-phosphocholine. Meanwhile, Y sperm had greater levels of acetyl-L-carnitine. The pathway analysis showed that X sperm primarily utilized carbohydrate and fat processing through glycolysis and phosphocholine pathways to maintain their movement and membrane integrity. On the other hand, Y sperm mainly use a carnitine-based energy pathway to support their faster and straighter movement. The identified metabolites can serve as reliable biomarkers to aid in laboratory quality control checks and enhance the quality of semen extenders. This outcome gives a good way to make sexed semen more stable and reliable in tropical dairy farming conditions.
Effect of Ejaculation Frequency on Semen Quality of Bali Cattle Tamiyadi, A.; Yusuf, M.; Sahiruddin, S.; Diansyah, A.M.; Masturi, M.; Nurgina, A.T.
Hasanuddin Journal of Animal Science (HAJAS) Vol. 8 No. 1 (2026)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.v8i1.45734

Abstract

Semen quality is influenced by the frequency of ejaculation, which is significant to semen characteristics. The purpose of this study was to analyze the effect of ejaculation frequency on the quality of fresh Bali cattle semen, which includes volume, motility, and concentration of spermatozoa at the AI Center Pucak Maros. This study used secondary data from production records (recording) derived from 5 Bali cattle males, with a frequency of ejaculation of 2 times. The results showed that the frequency of ejaculation was statistically significantly different (P<0.05) in semen volume in males 11442 and 11434; the percentage of motility was significantly different (P<0.05) in male 11539; and the concentration of spermatozoa was significantly different (P<0.05) in males 11442, 11434, and 11539. The correlation coefficients for the three quality parameters showed negative correlations between semen volume and motility (R=-0.531) and concentration (R=-0.414), whereas motility and concentration were positively correlated (R=0.740). In conclusion, the second ejaculation has an impact on increasing semen volume but will reduce the percentage of motility and concentration of spermatozoa. Keywords: ejaculation frequency, Balinese cattle, volume, motility, concentration
POLA KECERDASAN EMOSIONAL ANAK PASCA PEMBELAJARAN DARING Hesti Wahyu Anggraeni; Amara Qonita Nur zati; Masturi Masturi
Jurnal Prasasti Ilmu Vol. 3 No. 1 (2023): Jurnal Prasasti Ilmu
Publisher : Universitas Muria Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24176/jpi.v3i1.7512

Abstract

Tujuan pada penelitian ini yaitu untuk mengetahui pola kecerdasan emosional pada anak pasca pembelajaran daring. Penelitian ini merupakan jenis penelitian kualitatif naratif dengan pendekatan studi kasus yang dilaksanakan di Desa Sambung Kecamatan Gajah Kabupaten Demak. Teknik pengumpulan data dalam penelitian ini meliputi observasi, wawancara, dokumentasi, dan pencatatan. Teknik analisis data pada penelitian ini yaitu reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian menunjukkan terdapat lima pola kecerdasan emosional siswa yakni, kemampuan mengenali emosi diri, kemampuan mengelola emosi diri, kemampuan memotivasi diri, kemampuan mengenali emosi orang lain, dan kemampuan membina hubungan. 
Feasibility Analysis of Scientific Reasoning Skills Instruments for Prospective Science Teachers Using The Rasch Model Yuni Arfiani; Dwi Yulianti; Masturi Masturi; Sunyoto Eko Nugroho
Unnes Science Education Journal Vol. 14 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/usej.v14i2.25493

Abstract

This study aims to analyze the feasibility of a scientific reasoning ability test instrument for prospective science teachers using the Rasch model. The instrument developed refers to Lawson's Classroom Test of Scientific Reasoning (LCTSR) indicators, focusing on scientific reasoning abilities, including variable control, combinatorial, probability, relational, and proportional thinking. The analysis was carried out using the Rasch Model with the WINSTEPS Version 5.7.3.0 application to test the validity, reliability, and characteristics of the test items. The feasibility test was carried out on 40 multiple-choice questions, tested on 110 test samples. The analysis results showed that 25 of the 40 questions were valid and covered all indicators of problem-solving skills. The test instrument also has a high level of internal consistency with a person reliability value of 0.84 and an item reliability of 0.95. Analysis of the level of difficulty of the test items showed a balanced distribution between very difficult, difficult, moderate, and easy questions. No items were detected as biased, indicating that this instrument is fair and can be used without favoring one group of individuals. Overall, the developed problem-solving skills test instrument is valid and reliable for use on prospective science teachers, assisting educators in evaluating students' problem-solving skills, providing constructive feedback, and improving the quality of learning in science.  
Visual Literacy Research Trends in Science Learning 2014-2024 Puteri Indah Qoiru Nisa; Masturi Masturi
Educia Journal Vol. 4 No. 2 (2026): Educia Journal
Publisher : Educia Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71435/741705

Abstract

Visual literacy is a crucial competency in science learning in the digital era because it helps students understand abstract scientific concepts through various visual representations. However, the development of research on visual literacy in science learning has not been comprehensively mapped. This study aims to analyze trends in visual literacy research in science learning during the 2014–2024 period based on publication distribution, research methods, subject characteristics, subject context, and geographic distribution. The study used the Systematic Literature Review (SLR) method with reference to the PRISMA guidelines. Data were collected through Publish or Perish software connected to the Google Scholar database using the keywords visual literacy AND science education and visual skills AND science learning. Based on a selection process according to inclusion and exclusion criteria, 37 articles were obtained and analyzed descriptively and thematically. The results of the study indicate that visual literacy research publications have increased in the past five years, indicating increasing attention to this topic. Mixed-method and quasi-experimental approaches are the most commonly used methods. Research subjects are dominated by undergraduate and high school students, while the research context is mostly conducted in biology, followed by integrated science and chemistry. Geographically, research is concentrated in Asia and the Americas, with Indonesia and the United States as the primary contributors. These findings indicate that visual literacy is increasingly recognized as a critical component of 21st-century science learning. However, further research is needed across educational levels, disciplines, and geographic regions to support the development of more effective, inclusive, and visually-based learning strategies.
Co-Authors - Sutarti, - A. Cristina A. Setiawan A.A. Ketut Agung Cahyawan W Adam, A. A. S. Adelina Ryan Candra Dewi Agus Ulin Nuha Ahmad Khoiri Alfian, A. M. Amara Qonita Nur zati Amrullah, M. F. Andhy Setiawan Andhy Setiawan Anton Winarto Arif Hermawan Arista Rahayu Bambang Subali Budi Astuti Dewi Risma Winari Dian Novita Sari Dian Wahid Hermawan Dian Wahid Hermawan Diansyah, A. M. Diansyah, A.M. Dwi Yulianti Ema Chintya Permatasari Emi Sulistri Emi Sulistri, Emi Frilisa Dliyaul Haya Galuh Ayu Riyanti H. Aliah, H. Hamdan Hamdan Haqiqi, Arghob Khofya Harra Hau, Rambu Ririnsia Hartono Hartono Hartono Hartono Hasniah Aliah Hasniah Aliah Heru Damayanti Hesti Wahyu Anggraeni Husna Noor Mufida Husna Noor Mufida Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Indra Rahmatul 'Ula Irina Mei Risca Pratama Ivan Setia Arianto Landiana Etni Laos, Landiana Etni Lisa Puspitasari M. Abdullah M. Yusuf Mamik Indaryani Maya Puspita Sari Mikrajuddin Abdullah Mikrajuddin Abdullah Moh. Nashir Tsalatsin Moh. Nashir Tsalatsin, Moh. Nashir Muchsin Muh Syukri Ahsani Muh Syukri Ahsani Musa Dimyati N. Hindarto N. Istiana Nur Khoiri Nurgina, A.T. Nurlatifah, A. Nurulia Fajriani Obimita Ika Permatasari, Obimita Ika P. Dwijananti P. Marwoto Puteri Indah Qoiru Nisa Rahmat, R. Rizka Silviana Hartanti Rizki Riyansyah Rosyidatul Munawaroh Rosyidatul Munawaroh S. Rustad, S. S. Said S. Susilawati, S. Sahiruddin, S. Salvo Kahumbu Hau Shodiq Eko Ariyanto Siswanto Siswanto Siswanto Siswanto Soleman Dappa Talu Sucipto Suharto Linuwih Sujarwata Sujarwata SUKOWATI, DWI Sumarli Sumarli Sumarli Sumarli Sunarno Sunyoto Eko Nugroho, Sunyoto Eko Supriyadi Supriyadi Sutikno Madnasri T. Maulana, T. Tamiyadi, A. Ulya Santa Anugrahaini Vivi E. R. Husin Wijayanto, W. Xaphakdy, S. Yeni Purwiyantini Yuni Arfiani Yunita Putri Kusrinaningrum Yusri, A. N. H. S.