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Integrated Metabolomic and Functional Assessment of Sexed Frozen Semen in Holstein Friesian Bulls Yusuf, M.; Diansyah, A. M.; Sahiruddin, S.; Masturi, M.; Maulana, T.; Said, S.; Rahmat, R.; Alfian, A. M.; Adam, A. A. S.; Yusri, A. N. H. S.; Nurlatifah, A.; Amrullah, M. F.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.19

Abstract

This study examined the differences in sperm quality and metabolite profiles among X and Y sperm in Friesian Holstein bulls. It also tried to find specific metabolites that can help improve the accuracy of sexed semen use in humid tropical dairy farms. Semen samples from five bulls were separated by sex and tested for movement, live sperm count, shape, and membrane and acrosome health. The tests used Computer-Assisted Sperm Analysis (CASA) and regular microscope checks. Metabolite analysis was performed using LC-HRMS, followed by various statistical tests, pathway checks, and ROC curve analysis. The outcomes showed that X sperm had better movement and acrosome health, while Y sperm had stronger membranes, fewer shape problems, and moved faster. The untargeted metabolite study showed clear differences between the two groups. X sperm had more identified metabolites and greater average levels. The crucial metabolites found more in X sperm were D-(−)-fructose, L-(+)-lactic acid, and sn-glycero-3-phosphocholine. Meanwhile, Y sperm had greater levels of acetyl-L-carnitine. The pathway analysis showed that X sperm primarily utilized carbohydrate and fat processing through glycolysis and phosphocholine pathways to maintain their movement and membrane integrity. On the other hand, Y sperm mainly use a carnitine-based energy pathway to support their faster and straighter movement. The identified metabolites can serve as reliable biomarkers to aid in laboratory quality control checks and enhance the quality of semen extenders. This outcome gives a good way to make sexed semen more stable and reliable in tropical dairy farming conditions.
Co-Authors - Sucipto - Sutarti, - A. Cristina A. Setiawan A.A. Ketut Agung Cahyawan W Adam, A. A. S. Adelina Ryan Candra Dewi Agus Ulin Nuha Ahmad Khoiri Alfian, A. M. Amrullah, M. F. Andhy Setiawan Andhy Setiawan Anton Winarto Arghob Khofya Haqiqi Arif Hermawan Arista Rahayu Bambang Subali Budi Astuti Dewi Risma Winari Dian Novita Sari Dian Wahid Hermawan Dian Wahid Hermawan Diansyah, A. M. Ema Chintya Permatasari Emi Sulistri Emi Sulistri, Emi Frilisa Dliyaul Haya Galuh Ayu Riyanti H. Aliah, H. Hamdan Hamdan Harra Hau, Rambu Ririnsia Hartono Hartono Hartono Hartono Hasniah Aliah Hasniah Aliah Heru Damayanti Husna Noor Mufida Husna Noor Mufida Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Ian Yulianti Indra Rahmatul 'Ula Irina Mei Risca Pratama Ivan Setia Arianto Landiana Etni Laos, Landiana Etni Lisa Puspitasari M. Abdullah M. Yusuf Mamik Indaryani Maya Puspita Sari Mikrajuddin Abdullah Mikrajuddin Abdullah Moh. Nashir Tsalatsin Moh. Nashir Tsalatsin, Moh. Nashir Muchsin Muh Syukri Ahsani Muh Syukri Ahsani Musa Dimyati N. Hindarto N. Istiana Nur Khoiri Nurlatifah, A. Nurulia Fajriani Obimita Ika Permatasari, Obimita Ika P. Dwijananti P. Marwoto Rahmat, R. Rizka Silviana Hartanti Rizki Riyansyah Rosyidatul Munawaroh Rosyidatul Munawaroh S. Rustad, S. S. Said S. Susilawati, S. Sahiruddin, S. Salvo Kahumbu Hau Shodiq Eko Ariyanto Siswanto Siswanto Siswanto Siswanto Soleman Dappa Talu Suharto Linuwih Sujarwata Sujarwata SUKOWATI, DWI Sumarli Sumarli Sumarli Sumarli Sunarno - Sunarno Sunarno Supriyadi Supriyadi Sutikno Madnasri T. Maulana, T. Ulya Santa Anugrahaini Vivi E. Roshanty Husin Wijayanto, W. Xaphakdy, S. Yeni Purwiyantini Yunita Putri Kusrinaningrum Yusri, A. N. H. S.