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Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Tanor, Meity N; Abadi, Abdul Latief; Rahardjo, Bambang Tri; Pelealu, Jantje
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

Abstract

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).
Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Meity N Tanor; Abdul Latief Abadi; Bambang Tri Rahardjo; Jantje Pelealu
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

Abstract

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).
Co-Authors A. Nurfitriani Abdul Latief Abadi Alfrina Mewengkang Anatje Lihiang Arifin Arifin Arrijani - Aser Yalindua Assa, Evelyn P. A. Balansa, Feibi Rasti Bambang Tri Rahardjo Bambang Tri Rahardjo Bertje R.A. Sumayku Carolin Manuahe Caroline Manuahe Crescentia Mishi Juniorita Ngangi Danny Ch. Posumah Danny Ch. Posumah Dany Posumah Dea Debora Losoh Debby Jacqualine Jochebed Rayer Decky D. W. Kamagi Delfa Oktaverina Desi Rinnawati Sinaga Dino Rahardiyan Emma M Moko Fanny Nella Nanlohy Fauzan Sugeha Fricilia Leony Mey Jacob Gea, Aguslina Hasmiati Hasmiati Helen Joan Lawalata Herry Maurits Sumampouw Ika Okhtora Angelia Indah Tumelap Iren Natalia Simanjuntak Jantje Ngangi Jantje Pelealu Jantje Pelealu Jefry Jack Mamangkey Jelita Nainggolan Jenita, Elva Kasim, Yuriko Livana Dethris Rawung Lumapow, Kerol Marentek, Tifani Windie Mariana Rengkuan Masye Wurarah Meheda, Mariana Mokosuli Yermia Samuel Montela Waney Nangoy, Wiesye Nonny Manampiring Nova L.I.M Ogi Nuriyati Hadia Orbanus Naharia P. Gedoan, Sukmarayu Paputungan, Mifta Aulia Parura, Krisnawati Helmawati R. Kawuwung, Femmy Regina R. Butarbutar Rengkung, Prisilia Marlin Revolson Mege Rudi Alexander Repi Salawangi, Novia Semuel, Mokosuli Yeremia Simbar, Jessika Sri Dewinta Kurais Sukmarayu P. Gedoan Sumayku, Bertje R. A. Suprianto Sasea Suprianto Sasea Taulu, Marthy L. S. Taulu, Marthy S. Telew, Evanglita Tesalonika Yohana Wowat Tuegeh, Reinny Silvana Tumbel, Ferny M. Verawaty I.Y. Roring Vilia Emor Worotitjan, Regina Enjelina Yohanes Bery Mokalu Yubellian Yoru