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Tegillarca granosa shell combination with Vitis vinifera and fluoride in decreasing enamel microporosity Grace Caroline Setiawan; Adelia Tinisia; Muhammad Galang Adhinata Abdul Rahim; Fitria Rahmitasari; Widyasri Prananingrum
Dental Journal Vol. 57 No. 2 (2024): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i2.p131-138

Background: White spot lesion is a demineralization process indicated by the increased of enamel microporosity. A tegillarca granosa shell contains 98.7% calcium and Vitis vinifera contains phytochemical compounds with fluoride, which has a potential to stimulate remineralization. Purpose: To analyze the Tegillarca granosa shell combination with Vitis vinifera and fluoride in decreasing enamel microporosity. Methods: The cream was prepared by combining 10% and 20% Tegillarca granosa shell with 10 grams of Vitis vinifera extract and 100 mg of fluoride. The cream was tested beforehand for viscocity and pH. Furthermore, 16 premolars were etched and divided into four groups. Group 1 was smeared with placebo (negative control) and Group 2 was smeared with casein phosphopeptide-amorphous calcium phosphate (positive control). The other groups were smeared with cream 10% (Group 3) and 20% (Group 4) Tegillarca granosa shell combination with Vitis vinifera and fluoride. Teeth were treated three times a day for 30 minutes and soaked in artificial saliva. After 14 days, the enamel microporosity was carried out using a scanning electron microscope. The data was analyzed with one-way analysis of variance (ANOVA) test followed by post-hoc least significant difference (LSD). Results: The enamel microporosity showed significant difference between Group 1 and the other groups. There was no significant difference between Groups 2, 3, and 4 (p<0.05). Although there was no significant difference between Group 3 and 4, the lowest one was in Group 4 (p>0.05). Conclusion: The cream, prepared by combining Tegillarca granosa shell with Vitis vinifera and fluoride, is effective in decreasing the enamel microporosity.

The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Fitria Rahmitasari; Twi Agnita Cevanti; Puguh Bayu Prabowo; Sularsih Sularsih; Widyasri Prananingrum
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups

Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition Widyasri Prananingrum; Grace Caroline; Mohammad Basroni Rizal; Puguh Bayu Prabowo; Afif Fahwi Pratama; Muhammad Firdan Resaldi; Nindya Yuanita Annisa; Yusti Fadhilah; Rima Parwati Sari
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.247-256

Background: Biphasic calcium phosphate (BCP) is graft material contained HA and TCP. Tegilarca granosa shell is a natural source that may converted into BCP. This study aims to determine the composition and cytotoxicity of BCP synthesized from Tegilarca granosa shell used various hydrothermal hours and to evaluate the mechanical properties of BCP scaffold. Methods: Tegilarca granosa shell was converted into BCP using hydrothermal method at 200ËšC for 6h (Group 1); 9h (Group 2); and 12h (Group 3). The composition was determined by XRD and the cell viability were evaluated using MTT Assays. Each group was added with 20% gelatin ratio 50:50 (w/v) and freeze-dried to form scaffold. Scaffolds (Ã˜6mm x 4mm) were prepared for diametral tensile strength (DTS) test (n=6) and scaffolds (Ã˜7mm x 11mm) were used for compressive strength (CS) test (n=6). All data were analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The composition of BCP (HA/ TCP) at Group 1, Group 2, and Group 3 were 81.80%/14,10%; 87%/6%; and 72%/21%. The cell viabilities were good for all groups. The DTS and CS test showed there was a significant difference between Group 1 and Group 3 scaffold, meanwhile there was no significant differences between Group 2 and Group 3 scaffold. Group 3 scaffold showed the highest DTS and CS, 6.921 MPa and 1,233 MPa. Conclusion: The BCP composition were depent on hydrothermal hours. Although all scaffold groups were non-toxic, but BCP scaffold synthesized from Tegilarca granosa shell using hydrothermal for 12 hours showed the highest mechanical properties.

Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Kartika Zefanya; Fitria Rahmitasari; Widaningsih Widaningsih; Widyasri Prananingrum
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.

COMMUNITY EMPOWERMENT IN EFFORTS TO IMPROVE DENTAL AND ORAL HEALTH THROUGH TRAINING OF TRAINER ACTIVITIES IN “ANAK BANGSA ELEMENTARY SCHOOL” SURABAYA Kharinna Widowati; Caecilia Indarti; Rima Parwati Sari; Widyasri Prananingrum; Ghita Hadi Hollanda; Yulie Emilda; Kristanti Parasihni; Isidora Karsini; Monica Elidasari; Lita Agustia; Budi Santoso; IGAM Oka Lestari; Yoifah Rizka; Yongki Hadinata; Linda Rochyani
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 8 No. 1 (2024): MARET
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v8i1.2221

One way to develop health is community empowerment in the field of dental and oral health. Based on the 2018 Basic Health Research, it was reported that the prevalence of dental and oral problems in Indonesia is still very high. As many as 93% of school-aged children experience dental and oral problems. This is due to a lack of knowledge and lack of awareness of the Indonesian population regarding dental hygiene. In a series of activities for the 2023 National Dental Health Month, dental and oral health education programs are carried out in schools/School Health Programs. Method: The method is carried out using a cadre training program / Training of Trainers through zoom meetings for teachers and parents of students by providing dental and oral health material. Next, ask them to provide assistance with morning and evening toothbrushing activities for 21 days, teaching children about the benefits of good oral hygiene and showing them how to brush their teeth twice a day. It is preceded by a pretest and at the end of the delivery of the material a post test is given. The results of the evaluation of understanding of the material presented show that there is a significant difference in the pre-test and post-test of prospective cadres, which means that the cadres understand the material well. The new habit of brushing teeth has been quite successful from the uploaded photo documentation and students have recorded well for 21 days. This shows a change in behavior in maintaining dental and oral health.

Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Kartika Zefanya; Fitria Rahmitasari; Widaningsih Widaningsih; Widyasri Prananingrum
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.

The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Fitria Rahmitasari; Twi Agnita Cevanti; Puguh Bayu Prabowo; Sularsih Sularsih; Widyasri Prananingrum
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups

Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition Widyasri Prananingrum; Grace Caroline; Mohammad Basroni Rizal; Puguh Bayu Prabowo; Afif Fahwi Pratama; Muhammad Firdan Resaldi; Nindya Yuanita Annisa; Yusti Fadhilah; Rima Parwati Sari
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.247-256

Background: Biphasic calcium phosphate (BCP) is graft material contained HA and TCP. Tegilarca granosa shell is a natural source that may converted into BCP. This study aims to determine the composition and cytotoxicity of BCP synthesized from Tegilarca granosa shell used various hydrothermal hours and to evaluate the mechanical properties of BCP scaffold. Methods: Tegilarca granosa shell was converted into BCP using hydrothermal method at 200ËšC for 6h (Group 1); 9h (Group 2); and 12h (Group 3). The composition was determined by XRD and the cell viability were evaluated using MTT Assays. Each group was added with 20% gelatin ratio 50:50 (w/v) and freeze-dried to form scaffold. Scaffolds (Ã˜6mm x 4mm) were prepared for diametral tensile strength (DTS) test (n=6) and scaffolds (Ã˜7mm x 11mm) were used for compressive strength (CS) test (n=6). All data were analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The composition of BCP (HA/ TCP) at Group 1, Group 2, and Group 3 were 81.80%/14,10%; 87%/6%; and 72%/21%. The cell viabilities were good for all groups. The DTS and CS test showed there was a significant difference between Group 1 and Group 3 scaffold, meanwhile there was no significant differences between Group 2 and Group 3 scaffold. Group 3 scaffold showed the highest DTS and CS, 6.921 MPa and 1,233 MPa. Conclusion: The BCP composition were depent on hydrothermal hours. Although all scaffold groups were non-toxic, but BCP scaffold synthesized from Tegilarca granosa shell using hydrothermal for 12 hours showed the highest mechanical properties.

Pengaruh Metode Pencampuran Coupling Agent Terhadap Sitotoksisitas Komposit Serat Selulosa Sabut Kelapa / Coir Cevanti, Twi Agnita; Prananingrum, Widyasri; Soesilo, Diana
Jurnal Material Kedokteran Gigi Vol 11 No 1 (2022): JMKG Vol 11 No 1 Maret 2022
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v11i1.1032

Research about cellulose fiber from coconut fibers (Cocos nucifera L/coir) was currently done as an organic fillers for dental composite materials. DGEBA was chosen as a coupling agent to obtain covalent chemical bonds between BisGMA, TEDGMA matrices and coir cellulose fibers. The mixing method of DGEBA played an important role in the biocompatibility of the material. The ideal requirement for dental materials must be non-toxic through cytotoxicity tests. Objective to modify the synthesis method of coir-based cellulose as a natural fiber to optimize chemical bonding through polymerization reaction between fiber, coupling agent and matrix. Methods: There were 2 kinds of cellulose synthesis methods, which were (1) synthesis of cellulose solution using DGEBA and ethanol, (2) synthesis of cellulose solution using ethanol without DGEBA. Cytotoxicity test on pulp fibroblast cells with MTT Assay method on coir cellulose fiber composite samples from the two methods. Method 1 cell viability rate for 7 days immersion was 93.30855%, 14 days was 99.07063%, 21 days was 74.53532%. Method 2 obtained 64.68401% for 7 days immersion, 73.79182% for 14 days and 53.15985% for 21 days. Conclusion, the cellulose solution synthesis method using DGEBA and ethanol produces composites with better chemical bonds seen from the results of the cytotoxicity test

PENGARUH TRAINING OF TRAINER KESEHATAN GIGI DAN MULUT PADA GURU DAN ORANG TUA TERHADAP PERUBAHAN PERILAKU MENGGOSOK GIGI SISWA SISWI SDIT AL-USWAH Pargaputri, Agni Febrina; Andriani, Dwi; Parisihni, Kristanti; Priambodo, Nur Tsurayya; Saptaswari, Dianty; Elidasari, Monika; Fauzia, Bunga; Mulawarmanti, Dian; Sari, Rima Parwati; Kusumawardani, Chaterina Diyah Nanik; Setianingtyas, Dwi; Prananingrum, Widyasri; Nafi'ah; Laihad, Fanny M.; Teguh, Paulus Budi
MAJU : Indonesian Journal of Community Empowerment Vol. 1 No. 6 (2024): MAJU : Indonesian Journal of Community Empowerment, November 2024
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/ss5x9x45

Oral and dental health is an essential aspect of general health that must be maintained from an early age. This study aims to evaluate the impact of the Training of Trainer (ToT) program on dental and oral health for teachers and parents on changing tooth brushing behavior among students at SDIT Al-Uswah Surabaya. The methods used include surveys, training, evaluations, and observations of a tooth-brushing calendar filled out over 21 days. The results showed an increase in knowledge among teachers and parents, as well as positive changes in students' habits of brushing their teeth twice daily. These results highlight the importance of the role of teachers and parents in supporting dental and oral health promotion for children, which is expected to reduce the risk of dental caries in the future.

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