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Characteristics of chitosan from Penaeus monodon on chitosan-gelatin suspension viscosity Prananingrum, Widyasri; Setiawan, Grace Caroline; Putri, Vindya Meinda; Wahyuning, Baiq Nadia Risna; Rahmitasari, Fitria; Sularsih, Sularsih; Ramadhania, Nurina Rizka; Fatmawati, Sri
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 2 (2025): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i2.p135-143

Background: Chitosan synthesized from Penaeus monodon shells was developed into a chitosan-gelatin suspension as an injectable bone substitute for socket preservation. Purpose: To investigate the characteristics of chitosan from P. monodon shells and their influence on the viscosity of a chitosan-gelatin suspension. Methods: P. monodon shells from Tarakan Waters were prepared using three methods: Group 1) deproteinization-depigmentation-deacetylation, Group 2) demineralization-depigmentation-deacetylation, and Group 3) deproteinization-demineralization-depigmentation-deacetylation. The chitosan was characterized by morphology, moisture and ash content, molecular weight (MW), deacetylation degree (DD), and viscosity. This chitosan was made into a chitosan-gelatin suspension with a ratio of 45:55 (w/w%) (95 ml:110 ml). The differences in viscosity of the chitosan-gelatin suspension were determined using Kruskal–Wallis and Mann–Whitney tests. The effects of the chitosan’s MW and DD on the viscosity of the chitosan-gelatin suspension were analyzed using Spearman’s correlation. Results: Group 2 had the highest moisture content (10.63%), MW (159.68 kDa), viscosity of the chitosan powder (5.53 dPa.s), and viscosity of the chitosan-gelatin suspension (40.20 cps). Group 1 had the highest ash content (27.83%) and DD (93.72%). Group 3 showed the lowest ash content (1.06%), MW (37.12 kDa), and DD (86.22%), but it had good viscosity of the chitosan-gelatin suspension (37.25 cps). A significant difference in viscosity was found between the chitosan-gelatin suspension groups. Spearman’s correlation coefficients between the viscosity of the chitosan-gelatin suspension and MW and between the viscosity of the chitosan-gelatin suspension and DD were 0.389 and −0.195, respectively. Conclusion: The viscosity of a chitosan-gelatin suspension is influenced by the MW and DD of the chitosan powder. Chitosan from P. monodon can potentially be an injectable bone substitute in socket preservation.

Cocos nucifera l. (coir) fiber application as a filler and its effect on the volumetric shrinkage of flowable composite resin: an in vitro study cevanti, Twi agnita; Tameno, Yolanda Agustina; Mawarani, Lizda Johar; Akwan, Yulie Emilda; Prananingrum, Widyasri; Son, Meekyoung
Padjadjaran Journal of Dentistry Vol 37, No 1 (2025): March 2025
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol37no1.58864

Introduction: The use of composite resin as a restorative material has the disadvantage of experiencing volumetric shrinkage during polymerization, which can lead to restoration failure. Fiber-reinforced composites can reduce volumetric shrinkage in restorative materials. Coir fiber has the potential to replace synthetic fillers because its hollow fiber structure resembles foam, supporting a compact yet lightweight structure. This characteristic is expected to reduce the volumetric shrinkage of composite resin. This study aims to analyze the effect of coir fiber application as a filler on flowable composite resin on shrinkage, comparing it with flowable composite resin containing synthetic filler. Methods: The cellulose fiber used as a filler was synthesized from coir, while the matrix consisted of BisGMA and TEGDMA resins in a ratio 5:1. The composite was prepared with a coir fiber-to-matrix resin composition of 70:30, and polymerization was carried out by irradiation for 40 seconds. Volumetric shrinkage was tested 90 minutes after irradiation. The dimensions of the composite sample were measured from images captured using a digital microscope and analyzed with ImageJ software. The volume shrinkage was then calculated based on these dimensions. Shrinkage was further confirmed through SEM analysis of marginal adaptation tests. Results: The application of coir fiber as a filler effectively reduced the volumetric shrinkage of the composite compared to the composites with synthetic filler. Statistical analyses using the Mann-Whitney test results indicated that the type of filler in flowable composite resin had a significant effect, with a p-value of 0.047 (p<0.05). Conclusion: Application of coconut fiber as filler in flowable composite resin significantly reduce volumetric shrinkage, making it three times smaller than that of flowable composite resin with a synthetic filler.

The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups

Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition Prananingrum, Widyasri; Caroline, Grace; Rizal, Mohammad Basroni; Prabowo, Puguh Bayu; Pratama, Afif Fahwi; Resaldi, Muhammad Firdan; Annisa, Nindya Yuanita; Fadhilah, Yusti; Sari, Rima Parwati
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.247-256

Background: Biphasic calcium phosphate (BCP) is graft material contained HA and TCP. Tegilarca granosa shell is a natural source that may converted into BCP. This study aims to determine the composition and cytotoxicity of BCP synthesized from Tegilarca granosa shell used various hydrothermal hours and to evaluate the mechanical properties of BCP scaffold. Methods: Tegilarca granosa shell was converted into BCP using hydrothermal method at 200ËšC for 6h (Group 1); 9h (Group 2); and 12h (Group 3). The composition was determined by XRD and the cell viability were evaluated using MTT Assays. Each group was added with 20% gelatin ratio 50:50 (w/v) and freeze-dried to form scaffold. Scaffolds (Ã˜6mm x 4mm) were prepared for diametral tensile strength (DTS) test (n=6) and scaffolds (Ã˜7mm x 11mm) were used for compressive strength (CS) test (n=6). All data were analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The composition of BCP (HA/ TCP) at Group 1, Group 2, and Group 3 were 81.80%/14,10%; 87%/6%; and 72%/21%. The cell viabilities were good for all groups. The DTS and CS test showed there was a significant difference between Group 1 and Group 3 scaffold, meanwhile there was no significant differences between Group 2 and Group 3 scaffold. Group 3 scaffold showed the highest DTS and CS, 6.921 MPa and 1,233 MPa. Conclusion: The BCP composition were depent on hydrothermal hours. Although all scaffold groups were non-toxic, but BCP scaffold synthesized from Tegilarca granosa shell using hydrothermal for 12 hours showed the highest mechanical properties.

Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.

SCHOOL HEALTH PROGRAM AS AN EDUCATIONAL FACILITY IN INCREASING KNOWLEDGE ABOUT DENTAL AND ORAL HEALTH AT BUNGA BANGSA ELEMENTARY SCHOOL Elizabeth Pranoto, Amelia; Fauziah, Yessy Andriani; Rahardjo, Pambudi; Agustia, Lita; Budi Teguh, Paulus; Santoso, Budi; Sularsih, Sularsih; Prananingrum, Widyasri; Ayu R, Dyah; Tsurayya, Nur; Widowati, Kharinna; Hadinata, Yongki
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 7 No. 3 (2023): SEPTEMBER
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v7i3.1700

Dental and oral health in Indonesia is still a health problem that requires attention. To address dental and oral health problems in Indonesia, particularly in school-age and adult groups, it is necessary to carry out promotive-preventive activities by inviting local universities and business entities that focus on dental and oral health. The most common dental and oral diseases are caries and periodontal disease. One of the strategies of the Ministry of Health of the Republic of Indonesia in reducing the number of dental caries sufferers is by declaring the "Carry Free Indonesia 2030" program. The lack of knowledge and awareness of the Indonesian population regarding dental hygiene and the difficulty of reaching information due to the geographical conditions in several regions in Indonesia which make it impossible to hamper the distribution of education. The Training of Trainer Program targets teachers and parents for 21 days of morning and evening toothbrushes, teaching children and adults about the benefits of good oral hygiene and showing them how to brush their teeth twice a day with fluoride toothpaste. This program aims to provide comprehensive, applicable and sustainable education and knowledge about dental and oral health to school-age children in Indonesia.

COMMUNITY EMPOWERMENT IN EFFORTS TO IMPROVE DENTAL AND ORAL HEALTH THROUGH TRAINING OF TRAINER ACTIVITIES IN “ANAK BANGSA ELEMENTARY SCHOOL” SURABAYA Widowati, Kharinna; Indarti, Caecilia; Sari, Rima Parwati; Prananingrum, Widyasri; Hollanda, Ghita Hadi; Emilda, Yulie; Parasihni, Kristanti; Karsini, Isidora; Elidasari, Monica; Agustia, Lita; Santoso, Budi; Lestari, IGAM Oka; Rizka, Yoifah; Hadinata, Yongki; Rochyani, Linda
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 8 No. 1 (2024): MARET
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v8i1.2221

One way to develop health is community empowerment in the field of dental and oral health. Based on the 2018 Basic Health Research, it was reported that the prevalence of dental and oral problems in Indonesia is still very high. As many as 93% of school-aged children experience dental and oral problems. This is due to a lack of knowledge and lack of awareness of the Indonesian population regarding dental hygiene. In a series of activities for the 2023 National Dental Health Month, dental and oral health education programs are carried out in schools/School Health Programs. Method: The method is carried out using a cadre training program / Training of Trainers through zoom meetings for teachers and parents of students by providing dental and oral health material. Next, ask them to provide assistance with morning and evening toothbrushing activities for 21 days, teaching children about the benefits of good oral hygiene and showing them how to brush their teeth twice a day. It is preceded by a pretest and at the end of the delivery of the material a post test is given. The results of the evaluation of understanding of the material presented show that there is a significant difference in the pre-test and post-test of prospective cadres, which means that the cadres understand the material well. The new habit of brushing teeth has been quite successful from the uploaded photo documentation and students have recorded well for 21 days. This shows a change in behavior in maintaining dental and oral health.

Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.

The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups

Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition Prananingrum, Widyasri; Caroline, Grace; Rizal, Mohammad Basroni; Prabowo, Puguh Bayu; Pratama, Afif Fahwi; Resaldi, Muhammad Firdan; Annisa, Nindya Yuanita; Fadhilah, Yusti; Sari, Rima Parwati
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.247-256

Background: Biphasic calcium phosphate (BCP) is graft material contained HA and TCP. Tegilarca granosa shell is a natural source that may converted into BCP. This study aims to determine the composition and cytotoxicity of BCP synthesized from Tegilarca granosa shell used various hydrothermal hours and to evaluate the mechanical properties of BCP scaffold. Methods: Tegilarca granosa shell was converted into BCP using hydrothermal method at 200ËšC for 6h (Group 1); 9h (Group 2); and 12h (Group 3). The composition was determined by XRD and the cell viability were evaluated using MTT Assays. Each group was added with 20% gelatin ratio 50:50 (w/v) and freeze-dried to form scaffold. Scaffolds (Ã˜6mm x 4mm) were prepared for diametral tensile strength (DTS) test (n=6) and scaffolds (Ã˜7mm x 11mm) were used for compressive strength (CS) test (n=6). All data were analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The composition of BCP (HA/ TCP) at Group 1, Group 2, and Group 3 were 81.80%/14,10%; 87%/6%; and 72%/21%. The cell viabilities were good for all groups. The DTS and CS test showed there was a significant difference between Group 1 and Group 3 scaffold, meanwhile there was no significant differences between Group 2 and Group 3 scaffold. Group 3 scaffold showed the highest DTS and CS, 6.921 MPa and 1,233 MPa. Conclusion: The BCP composition were depent on hydrothermal hours. Although all scaffold groups were non-toxic, but BCP scaffold synthesized from Tegilarca granosa shell using hydrothermal for 12 hours showed the highest mechanical properties.

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