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Pertumbuhan lobster air tawar Cherax quadricarinatus (Von Martens, 1868) yang diberi pakan Keong Tutut Jawa Filopaludina javanica (Von Dem Busch, 1844) Erwin Wiradita Mamonto; Winda Mercedes Mingkid; Revol D. Monijung; Henneke Pangkey; Nego Elvis Bataragoa
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 1 (2023)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.11.1.2023.42566

Abstract

The research was carried out to investigate the best amount of large freshwater snail (Filopaludina javanica) meat as feed for the growth and survivals (SR) of freshwater lobster C. quadricarinatus.  The size of freshwater lobster used in this study was 1.70 – 23.90 gr.  Lobsters were kept in aquariums measuring 30 x 30 x 40 cm for 30 days. The experimental design used was a completely randomized design (CRD) with three treatments and three replications that is A (4%), B (7%) and C (10%) of feed. Data analysis was used linier regression. The results showed the (R2) marks close to 1 means that the time of experiment effects the growth of lobster in all treatments, 4% feed (R2 =0,8919), 7% (R2=0,9942) and 10%(R2=0,9484, where the highest coefficient regression was treatment B (0,5818) followed by treatment A (0,4625) and C (0,42), respectively.  The highest survival rate was found in treatment B (91.7%), treatment A (75%) and C (66.7%), respectively.  Results of analysis showed that the size and the length of time of experiment effects the time of growth and survival.
Ekstrak daun kedondong laut (Polyscias fruticosa) sebagai modulator imun benih ikan nila (Oreochromis niloticus) Dahlia Malau; Reiny A. Tumbol; Sammy N. J. Longdong; Reni L. Kreckhoff; Winda M. Mingkid; Edwin L. A. Ngangi
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.47718

Abstract

Disease control using medicinal plants is an alternative to antibiotics and chemicals in aquaculture. One type of plant with the potential for health management is Polyscias fruticosa or sea kedondong which contains flavonoids, saponins, methanol, and tannins that are efficacious as antihistamines, antioxidants, antivirals, antibacterials, anti-inflammatories, and anti-cancer. The aim of the study was to examine the effect of kedondong laut leaf extract on increasing the phagocytic activity of tilapia (Oreochromis niloticus), and to determine the appropriate concentration of kedondong laut leaf extract to increase the phagocytic activity of tilapia (O. niloticus) seeds. The leaves of kedondong laut are cleaned, washed, and dried for 14 days at room temperature and then ground using a blender to obtain an extract of coarse flour. The flour was extracted using the maceration method with 70% alcohol solvent with a ratio of coarse flour and the solvent 1:5. After 24 hours of soaking, the extract was filtered using Whatman paper No. 42, and the dregs were soaked again with the same volume of solvent for the same soaking time and filtered again. This process is done 3 times until the extract looks clear. The extract was then concentrated using a rotary evaporator to obtain a concentrated extract of about 30% of the initial total extract. The concentrated extract is then sprayed evenly using a sprayer into the feed according to the set treatment dose. The research design used was Completely Randomized with 5 treatments, each with 3 replications. The treatments used were A: 0%, B: 1%, C: 2%, D: 3%, and E: 4% extract/kg feed. Treatment feed was given for 4 weeks at a dose of 5%/body weight/day, and the frequency of feeding was 2 times a day at 08:00 and 16:00. The results showed that the administration of sea kedongong leaf extract had a very significant effect on the phagocytic activity of tilapia (p<0.01). The best results were achieved in fish treated with B (1% extract/kg) for 28 days. Keywords: medicinal plants, maceration, immune modulators, phagocytosis activity, disease control
The Identification Of Morphometric Characteristics And Fattenning Of Mud Crabs (Scylla spp.) In Para Island, Tatoareng District, Sangihe Islands Regency, North Sulawesi Province Aditya Sasamu; Winda M. Mingkid; Revol D. Monijung
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.21491

Abstract

The purpose of this study was to identify the morphometric characters of mangrove crabs Scylla spp. and to grow them on the island of Para Sangihe Islands Regency, North Sulawesi Province. There were 2 types of crabs found during crab’s collection. However, the most abundant one was Scylla serrata while the olivacea is only one tail. The results of this study indicate that mangrove crabs that were kept for 2 weeks feed on trash fish three times a day experience growth. Based on the results of the study, it can be concluded that: the type of crab found on Pulau Para, Tatoareng District, Sangihe Islands Regency, North Sulawesi Province were mostly S. serrata (34 tails) and one tail of S. olivacea. The fattening of mangrove crabs showed results on the body weight and carapace length.Further research on mangrove crabs needs to be done on different aspects such as preferable food and time of the abundancy.Keywords: Crab, Scylla spp., identification, fattening. ABSTRAK       Tujuan dari penelitian ini adalah untuk mengidentifikasi karakter morfometrik kepiting bakau Scylla spp dan cara penggemukannya di Pulau Para Kabupaten Kepulauan Sangihe Provinsi Sulawesi Utara. Dua jenis kepiting bakau ditemukan saat pengumpulan data, namun yang ditemukan paling banyak adalah Scylla serrata, sedangkan S. olivacea hanya 1 ekor saja.Hasil penelitian ini menunjukan bahwa kepiting bakau yang dipelihara selama 2 minggu dengan diberi pakan ikan rucah sebanyak tiga kali sehari mengalami pertumbuhan. Berdasarkan hasil penelitian dapat disimpulkan bahwa: jenis kepiting yang ditemukan di Pulau Para Kecamatan Tatoareng Kabupaten Kepulauan Sangihe Provinsi Sulawesi Utara adalah Scylla serrata (34 ekor) dan S. olivacea (1 ekor). Penggemukan kepiting bakau dengan pakan ikan rucah tiga kali sehari menunjukan adanya pertumbuhan berat badan dan panjang lebar karapas.     Penelitian lanjutan tentang kepiting bakau perlu dilakukan untuk melihat berbagai aspek seperti makanan kesukaan dan waktu kelimpahanya.Kata kunci : Kepiting, Scylla spp, identifikasi, penggemukan.
Substitusi tepung cangkang Kijing Taiwan (Sinanodonta woodiana) dalam pelet komersial sebagai sumber mineral bagi pertumbuhan benih ikan Nila (Oreochromis niloticus) Metlin Boroallo; Cyska Lumenta; Ockstan J. Kalesaran; Jeffrie F. Mokolensang; Hengky J. Sinjal; Winda M. Mingkid
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.48248

Abstract

Penggunaan tepung cangkang kijing Taiwan sebagai sumber mineral bagi pertumbuhan benih ikan nila merupakan upaya untuk mengurangi limbah cangkang kijing Taiwan yang belum dimanfaatkan dengan baik oleh masyarakat. Penelitian ini bertujuan untuk menelaah apakah substitusi tepung cangkang kijing Taiwan dengan dosis berbeda berpengaruh nyata terhadap pertumbuhan mutlak dan pertumbuhan nisbi benih ikan nila, menentukan perlakuan yang memiliki tingkat pertumbuhan mutlak dan pertumbuhan nisbi tertinggi dan menetapkan nilai ratio konvesi pakan (FCR) dan efisiensi pakan terbaik. Ikan uji yang digunakan adalah benih ikan nila berukuran 1-2 gram. Pakan uji yang digunakan adalah pelet komersial yang disubstitusi tepung cangkang kijing Taiwan. Wadah pemeliharaan yang digunakan adalah wadah plastik sebanyak 12 buah. Sebelum penelitian, ikan uji diaklimatisasi selama 7 hari, selanjutnya, ikan ditebar kedalam wadah pemeliharaan sebanyak 5 ekor/wadah yang diisi air sebanyak 10 liter, ikan diberi pakan 3 kali sehari yaitu pukut 08.00, 12.00 dan 14.00 WITA secara ad libitum.  Rancangan penelitian yang digunakan adalah acak lengkap dengan 4 perlakuan dan 3 kali ulangan. Perlakuan yang digunakan adalah substitusi A (0%), B (2%), C (4%) dan D (6%) tepung cangkang kijing Taiwan. Data yang diamati adalah pertumbuhan mutlak, nisbi, kelangsungan hidup, ratio konvesi pakan) dan efisiensi pakan. Hasil penelitian menunjukkan bahwa substitusi tepung cangkang kijing Taiwan berpengaruh nyata terhadap pertumbuhan mutlak, pertumbuhan nisbi, ratio konvesi pakan dan nilai efisiensi pakan, tetapi tidak berpengaruh nyata terhadap kelangsungan hidup benih ikan nila. Sebagai kesimpulan pertumbuhan mutlak dan nisbi terbaik terdapat pada perlakuan C (4%) yaitu 3,38 gram dan 219,76%, nilai ratio konvesi pakan dan efisiensi pakan terbaik juga diperoleh pada perlakuan C (4%) dengan nilai 1,22 dan 82,93%. Kata kunci: pertumbuhan mutlak, pertumbuhan nisbi, kelangsungan hidup, ratio konversi pakan, efisiensi pakan
Pertumbuhan lobster air tawar (Cherax quadricarinatus) (von Martens, 1868) yang diberi pakan eceng gondok Pontederia crassipes dan Keong Mas, Pomacea canaliculata (Lammark,1819) Junaidi Asnawi; Winda M. Mingkid; Henneke Pangkey; Cyska Lumenta; Jeti K. Rangan
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.48265

Abstract

Penelitian ini bertujuan untuk menganalisis pertumbuhan mutlak, laju pertumbuhan harian dan menghitung tingkat kelangsungan hidup (SR) udang karang (Cherax quadricarinatus) melalui aplikasi eceng gondok Pontederia crassipes dengan keong mas dengan dosis berbeda yang dipelihara dalam wadah akuarium. . Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan diulang sebanyak 3 kali. Lobster air tawar diambil dari Danau Tondano dengan kisaran bobot 15 – 29 gram sebanyak 40 ekor. Pakan gabungan yang diberikan pakan eceng gondok dan keong mas sebanyak 20% dari total bobot lobster. Pemberian pakan dilakukan dua kali sehari, yaitu pada pukul 08.00 WIB dan 17.00 WIB, masing-masing sebanyak 10% pada pagi hari dan 10% pada sore hari. Pengukuran yang dilakukan dan data yang diamati dari penelitian ini adalah: pertambahan berat badan mutlak individu yang diukur setiap satu minggu sampai akhir masa pengamatan, laju pertumbuhan harian dan persentase individu yang hidup sampai akhir masa pengamatan (1 bulan). ). Hasil penelitian menunjukkan rata-rata pertambahan berat badan mutlak pada perlakuan A (2,61%), B (3,66%), dan C (4,03%) sedangkan laju pertumbuhan harian pada masing-masing perlakuan adalah perlakuan A (0,36%), B (0,54%). ), dan C (0,59%). Tingkat kelangsungan hidup menunjukkan bahwa perlakuan A dan B memiliki tingkat kelangsungan hidup tertinggi (92%) dan terendah ditemukan pada perlakuan D (75%). Hasil analisis varians pertumbuhan berat mutlak dan laju pertumbuhan harian menunjukkan bahwa perbedaan komposisi eceng gondok dan keong mas sebagai pakan lobster air tawar tidak berpengaruh nyata terhadap perbedaan pertumbuhan lobster air tawar. Kata kunci: budidaya, kelangsungan hidup, Danau Tondano, pakan ikan, kualitas air
Utilization of life feed Alona sp., Boiled Egg Yolk and Commercial Feed Toward Survival Rate of Betta Fish Larvae Mikraim J. Kaseger; Henneke Pangkey; Diane J. Kusen; Henky Manoppo; Winda M. Mingkid; Nego E. Bataragoa
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.23651

Abstract

The purpose of this study was to find out the survival rates of betta fish larvae (cupang) that were given life feed Alona sp., boiled egg yolk and commercial feed for 21 days. Data analysis was performed by one-way ANOVA, and continued with BNJ (Tukey) test. The result of the survival rate is as follow treatment with life feed Alona sp. 56%, treatment with boiled egg yolk 26%, and treatment with commercial feed 0%. The results of statistical tests show there are significant differences.  Significant value p <.0002 (<.05). Water quality parameter measured during the study was temperature of 26 ºC.Keywords: betta fish larvae, life feed, Alona sp., survival rateABSTRAKTujuan penelitian ini adalah untuk mendapatkan laju kelangsungan hidup larva ikan betta (cupang) yang dibeerikan pakan alami Alona sp., rebusan kuning telur dan pakan komersil selama 21 hari.  Analisis data dilakukan dengan ANOVA one way, dan dilanjutkan dengan uji BNJ (Tukey).  Hasil laju kelangsungan hidup adalah sebagai berikut, perlakuan dengan pakan alami Alona sp. 56%, perlakuan dengan rebusan kuning telur 26%, dan perlakuan dengan pakan komersil 0%.  Hasil uji statistik menunjukan adanya perbedaan yang nyata.  Nilai signifikan p <.0002 (<.05).  Parameter kualitas air yang diukur selama penelitian adalah suhu 26ºC.Keywords: larva ikan betta, pakan alami, Alona sp., kelangsungan hidup
The Survival Rate and Growth of Juvenile Crayfish (Cherax quadricarinatus) With Different Types of Shelter Jimmy Mamuaya; Winda M. Mingkid; Ockstan J. Kalesaran; Hengky J. Sinjal; Reiny A. Tumbol; John L. Tombokan
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.24510

Abstract

An experiment was designed to assess the relative performance of three shelter types on the survival and growth of red-claw crayfish (Cherax quadricarinatus).  A 13 days old juvenile crayfish with an average length of 10 mm were cultured in 400 ml water of glass bowl and were provided with one of 3 types of shelter over 19 days.  The various shelter types assessed were dried coconut leaves, bamboo and Hydrilla.  Juveniles in each bowl were fed with Artemia twice a day morning and in the afternoon. There was no significant effect (P>0.05) of shelter type on the survival rate; however, there was a significant effect (P<0.05) on growth with dried coconut leaves (5.33%) performing significantly better than Hydrilla (2.33%) and bamboo (2.00%).  This experiment showed that the shelter types provided had a significant effect on the growth but not in their survival.Keywords: Survival rate, Growth, juvenile Cherax quadricarinatus, ShelterABSTRAKTujuan penelitian ini adalah untuk menganalisis sintasan hidup dan  pertumbuhan  juvenil lobster air tawar (C. quadricarinatus) dengan shelter yang berbeda. Juvenil lobster yang berumur 13 hari dengan panjang rata rata 10 mm dipelihara dalam wadah toples kaca dengan diberikan ketiga perlakuan selama 19 hari.  Ketiga jenis perlakuan adalah daun kelapa kering, bambu dan Hydrilla.  Juvenil diberi pakan Artemia 2 kali sehari yaitu pada pagi dan sore hari.  Hasil penelitian menunjukkan ketiga perlakuan tidak berbeda nyata terhadap sintasan hidup (P>0.05), namun berbeda nyata (P< 0.05 ) untuk pertumbuhan dengan daun kelapa kering (5.33%), Hydrilla (2.33%) dan bambu (2%).     Penelitian ini menunjukkan bahwa jenis shelter memberikan pengaruh nyata  terhadap pertumbuhan namun tidak berbeda nyata untuk sintasan hidup.Kata kunci: Sintasan hidup, Pertumbuhan, Juvenil Cherax quadricarinatus, Shelter
Inventarisasi dan identifikasi ektoparasit yang menginfeksi benih ikan Nila (Oreochromis niloticus) Massora Dudung; Reni L. Kreckhoff; Reiny A. Tumbol; Sammy N.J. Longdong; Winda M. Mingkid
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.49945

Abstract

This study aimed to identify the parasites in tilapia seeds cultivated by the Matelenteng Fish Cultivation Group in Tumaluntung Village, as well as to analyze the prevalence, incidence, dominance index and preference level of parasites in tilapia seeds. This research was conducted from May to July 2023 in Tumaluntung Village, Kauditan District, North Minahasa Regency, North Sulawesi Province. Parasite examination was carried out on 15 samples of tilapia seeds which included the skin, fins, and gills of the fish. The fish samples were taken from the Matelenteng fish cultivation location in Tumaluntung Village, Kauditan District, North Sulawesi Province. The fish were taken from 1 breeding pond and put into plastic bags containing water, then given oxygen, and then brought to the Aquaculture Technology Laboratory of FPIK Unsrat for examination. Before the examination was carried out, the test fish were put into a bucket and given aeration so that the tilapia seeds remained alive until they were examined. Next, the length of the tilapia seeds was measured using a ruler. Examination of the tilapia seed samples was carried out using the smear preparation method where the examined organ was scraped, and the results of the scraping were placed in a Petri dish before being smeared on a prepared glass slide. Parasite examination was conducted using a microscope with a magnification of 100x. The inventory and identification results found 2 types of parasites, namely Epistylis sp. with 13 individuals in 3 samples of tilapia seeds and Dactylogyrus sp. with 2 individuals in 2 samples of tilapia seeds infecting the tilapia seeds. The prevalence rate of Epistylis sp. parasites was obtained at 33.33% or generally, there is a mild infection. Dactylogyrus sp. with a prevalence rate of 13.33% is classified as often infected with an incidence rate at a very mild level. The preference level of parasites obtained a value of X2 hit 3.74 < X2 tab 9.210 indicating a difference in the preference level of parasites in the tilapia seed body organs. Keywords: Ectoparasites, prevalence, incidence, preference level
Kajian kelayakan lokasi budidaya lobster (Panulirus spp.) di Perairan Desa Pulisan Noni Saliwati Sarifudin; Winda M. Mingkid; Hariyani Sambali; Suzanne L. Undap; Sammy N.J. Longdong; Ockstan J. Kalesaran
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 2 (2023): April - Desember
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.v11i2.50610

Abstract

The aim of this study was to determine the feasibility of the location for cultivating spiny lobsters (Panulirus spp.) in Pulisan Village waters, East Likupang District, North Minahasa Regency. This research was an explorative and descriptive research using primary and secondary data. Primary data was collected through direct observation, site surveys, and interviews, while secondary data was collected from various available sources such as reports, research, books guidebooks, citing records in of Pulisan Village, East Likupang District Office, and various literature.  Data were analyzed using descriptive analysis and internal and external factor analysis (SWOT). The results of the analysis showed that the location of spiny lobster cultivation met the SNI 8116 (2015) standard regarding sand spiny lobster (Panulirus pomarus) production. The strength–opportunity strategy is to protect the location and its surroundings where the existing habitat of the spiny lobster occurs, so the production will be gained and therefore the market price will be high.  The weakness–opportunity strategy is how government involvement in supporting the development of spiny lobster aquaculture.  Next, the strength–threat strategy is to maximize the use of high technology to overcome the unpredictable weather and season.  Finally, the weakness–threat strategy is to inspire and encourage local people about the benefits of doing spiny lobster aquaculture and how to overcome any problems.  Keywords: SWOT, water quality, aquaculture
Kelimpahan Vibrio spp. di Perairan Pesisir Kabupaten Halmahera Barat Tamrin Tamrin; Joshian Nicolas William Schaduw; Haryani Sambali; Adnan Sjaltout Wantasen; Desy Maria Helena Mantiri; Rene Charles Kepel; Winda Mercedes Mingkid; Ockstan Jurike Kalesaran; Nurhalis Wahidin; Muhammad Aris; Taufiq Abdullah
Juvenil Vol 5, No 3: Agustus (2024)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v5i3.23996

Abstract

ABSTRAKVibrio spp. adalah bakteri gram negatif yang biasa ditemukan di lingkungan estuari, pesisir, dan laut. Vibrio spp. merupakan patogen utama bagi kesehatan manusia dan organisme akuatik. Memahami kelimpahan Vibrio spp. sangat penting untuk dilakukan mengingat resiko terhadap kesehatan manusia, potensi kerusakan lingkungan serta ekonomi. Penelitian ini bertujuan mengeksplorasi kelimpahan Vibrio spp. di perairan pesisir Kabupaten Halmahera Barat. Terdapat sembilan kawasan pengamatan, yaitu Toniku, Tewe, Dodinga, Tuada, Porniti, Payo, Bobo, Sahu, dan Ibu. Sampel air dari bagian permukaan perairan sebanyak 100 ml dimasukan ke dalam botol steril. Pengamatan kelimpahan Vibrio spp. dilakukan dengan metode total plate count (TPC) pada media agar thiosulfate citrate bile salt sucrose (TCBS) yang diinkubasi selama 24 jam pada suhu 28°C. Hasil penelitian ini mengungkapkan Vibrio spp. pada perairan pesisir Kabupaten Halmahera Barat terdapat dalam 2 kelompok koloni, yaitu koloni hijau dengan kelimpahan 1,80 x 102 - 5,00 x 104 CFU/mL dan koloni kuning 2,20 x 103 - 3,08 x 105 CFU/mL. Kesimpulan penelitian ini adalah kelimpahan Vibrio spp. pada sembilan kawasan berkisar antara 102 - 105 CFU/mL.Kata kunci: Bakteri Vibrio spp., Koloni hijau, Koloni kuning, Total plate count.ABSTRACTVibrio spp. are gram-negative bacteria commonly found in estuary, coastal, and marine environments. Vibrio spp. are major pathogens to human health and aquatic organisms. Understanding the abundance of Vibrio spp. is very important given the risk to human health andpotential environmental and economic damage. This study explores the abundance of Vibrio spp. in the coastal waters of West Halmahera Regency. There were nine observation areas: namely Toniku, Tewe, Dodinga, Tuada, Porniti, Payo, Bobo, Sahu, and Ibu. Water samples from the surface waters, as much as 100 ml were put into sterile bottles. The abundance of Vibrio spp. was determined using the total plate count (TPC) method on thiosulfate citrate bile salt sucrose (TCBS) agar media, which were then incubated for 24 hours at 28°C. The results of this study revealed Vibrio spp. in coastal waters of West Halmahera Regency were found in 2 colony groups, namely green colonies with an abundance of 1.80 x 102 - 5.00 x 104 CFU/mL and yellow colonies 2.20 x 103 - 3.08 x 105 CFU/mL. The conclusion of this study is the abundance of Vibrio spp. in nine areas ranging from 102 - 105 CFU/mL.Keywords: Green colonies, Total plate count, Vibrio spp bacteria, Yellow colonies.
Co-Authors Aditya Sasamu Adnan Sjaltout Wantasen Adnan Wantasen Ali Djamhuri Antonius P Rumengan Bawekes, Frisley Rici Billy Th Wagey Calvyn F. A. Sondak, Calvyn F. A. Carolus P Paruntu Christo V S Aer, Christo V S Christo V.S Aer, Christo V.S Cyska Lumenta Cyska Lumenta Cyska Lumenta Dahlia Malau Diane J. Kusen Edwin D Ngangi Erly Y. Kaligis Erwin Wiradita Mamonto Farnis Boneka Fransine B. Manginsela Gompi, Wiranto Hariyani Sambali Haryani Sambali Hengky J. Sinjal Hengky J. Sinjal Hengky Sinjal Henky Manoppo Henneke Pangkey Henneke Pangkey Henneke Pangkey Janny Kusen Jeffrie F. Mokolensang Jeti K. Rangan Jhonly Solang Jimmy Mamuaya John L. Tombokan Joppy Mudeng Joshian Nicolas William Schaduw Juliaan Ch. Watung Julius Sampekalo Junaidi Asnawi Kreckhoff, Reni L. Kusen, Diane J. Lady D. Khartiono, Lady D. Mamuko, Nelsi Mantiri, Desy M. H Mardiana M Massora Dudung Medy Ompi Metlin Boroallo Mikraim J. Kaseger Mokoginta, Leyda H. Muhammad Aris Nego E. Bataragoa Nego Elvis Bataragoa Nickson J. Kawung Noni Saliwati Sarifudin Ockstan J Kalesaran Ockstan J. Kalesaran Ockstan J. Kalesaran Ockstan Jurike Kalesaran Ockstan Kalesaran Ockstan Kalesaran Paputungan, Fikri Ramses Nainggolan Reinol Jacobs Reiny A. Tumbol Reiny A. Tumbol Rene Charles Kepel, Rene Charles Revol D Monijung Revol D. Monijung Reygina B.S Roring Robert A. Bara Sambali, Hariyani Sammy N. J. Longdong Sammy N. J. Longdong Sammy N.J. Longdong Sammy N.J. Longdong Sarif Hidayat Silalahi, Torang J. P. Suria Darwisito Suzanne L. Undap Tamrin Tamrin Tamrin Taufiq Abdullah Tununu, Riky R. Veibe Warouw Wahidin, Nurhalis