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Kismiyati ,
Departemen Akuakultur, Fakultas Perikanan Dan Kelautan, Universitas Airlangga
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Pengaruh Pemberian Garam (NaCl) terhadap Kerusakan Telur Argulus Japonicus [Effect of Salt (NaCl) Against Damage of Argulus japonicus Egg] Kismiyati Kismiyati; Rakhmarani Nur Fatiza; Rahayu Kusdarwati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11633

Abstract

Abstract A. japonicus is a freshwater parasite which belonging as obligate parasite. Prevention and control of A. japonicus attacks still used an insecticides, such as organophosphates and other chemicals which are used as a control. The chemicals can damage environment and around the ecosystems. Salt (NaCl) can be used as control of A. japonicus infestation. NaCl is often used as A. japonicus control. The residues effect of using NaCl in fish culture is not known clearly. Therefore, NaCl can still be known as safety chemicals for aquaculture activities. This study aimed to inhibit hatching rate of A. japonicus. Definition the damage of egg is difference the egg condition from early condition and not whole return. In addition to, the damage process is refer to osmose dehydration. The study method was done by experiment with the experimental design was used Completely Randomized Design (CRD) with six treatments and five replicates. NaCl which used are 60 g/l, 70 g/l, 80 g/l, 90 g/l, 100 g/l and control. The main parameter is the percentage of hatching rate of A. japonicus. The support parameter on the research are otemperature (C), pH, DO (mg/l). Data analysis is using Analysis of Variance (ANOVA). The results showed that dipping with NaCl the influence of  damage of  A. japonicus eggs, but the analysis statistic did not provide the real difference treatment. 
Obyek Kesukaan untuk Penempelan Telur (Oviposisi) Ektoparasit Argulus japonicus [ Preference Of Oviposition Object Of Ectoparasite Argulus japonicus] Kismiyati Kismiyati; Nur Maulana Iskhaq; Juni Triastuti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11646

Abstract

Abstract A. japonicus is ectoparasite that attack only freshwater fish. The fish that was attacked by A. japonicus look skinny, always rub their body and appear some red spot that will manifest for second infection. Prevent and controll of A. japonicus, were used drug or chemical materials like insecticide. This compound can damage the environment and destroy surround ecosystem. Precisely metode to controll A. japonicus was focused for egg stage. The female habits of A. japonicus always lay her eggs (oviposition) on the surface object that surround on they habitat. The research using completely randomized design (CRD). The treatment on this research use 4 oviposition object like rock, wood, plants and PVC pipe. A rock that representative a hard and rough surface. Wood that representative rather solid state and irregular surface, plants with hard surface and representative a life organism (using Echinodorus palaefolius with wide and hard surface leaf), also PVC object that representative a hard and smooth surface. For comparator treatment, all oviposition object was collected in one treatment. All treatment were replicated at four times. Object was placed on bottom aquarium for use as oviposition object, to know preference of oviposition object by oviposition periode and totally eggs produced. ANOVA (analysis of variance) and Tukey-HSD range test were used to analyze data. Results showed that significant effect (p <0,05) on oviposition time and total eggs. The longest oviposition time and the higest eggs total was made by rock and pipe. The fastest oviposition time and the lowest eggs total was made by plants. Water quality during the study in optimum condition for A. japonicus and goldfish , temperature ranges from 26-28 0C, pH 7 and dissolved oxygen (DO) ranged at 5 mg/L.
Perubahan Histopatologi Kulit Ikan Koi (Cyprinus carpio) yang Terinfestasi Ichthyophthirius multifiliis Secara Kohabitasi [Skin Histopathology Alteration of Koi (Cyprinus carpio) With Ichthyophthirius multifiliis Infested Accordance Cohabitation] Gunanti Mahasri; Lyla Wulandari; Kismiyati Kismiyati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11663

Abstract

AbstractKoi (Cyprinus carpio) is one of the most famous freshwater ornamental fish which offers financial advantage. There are a lot of factors which have to be face when we're trying to cultivate or culture Koi, one of those factors is the disease. Parasite which always in freshwater fishes is Ichthyophthirius multifiliis. I. multifiliis penetrated through skin causing destruction ephitelium cells by cilia and by consequent causing a histopathological changing. The objectives of this research was to determine alteration of Koi (Cyprinus carpio) skin histopathology which infested by I. multifiliis through cohabotation. Cohabitation was done during 4 days with mixing 7 fishes infected with white spot disease to 100 healthy fishes. Koi that had been infested observing of pathology changes. And than slicing the skin organ to prepare histopatology slide from 3 sample in every level infestation. To detect the level infestation, parasite was divide into 3 level respectivelly are low level (1-5 parasite/slide), the medium level (6-10 parasites/slide) and the heavy level (more than 11 parasites/slide). Furthermore, damage level determination by histopatology was decided by scoring. Data analyzed with descriptive based on clinical simptom, and than, the skin histopatology which had been attacked was done by scoring. The results would be analyzed statistical with Kruskal Wallis test. If it is show the significant defference, the test would be continued with Z test 5% (Siegel, 1986).   The research showed that the fish skin Koi infestation by I. multifiliis there was histopatology changed shaped respectivelly epidermis proliferation, infiltration of inflammation cell and ephithel erosion. Based on histopatological changed skoring caused by I. multifiliis infestation from various infestation grade, there were 62.2% of Koi fish skin was epidermis proliferasi, 73.3% of one was infiltration of inflammation cell and 28.9% ephithel erosion. Water quality measurement showed that temperature and acidity in normal condition, respectively otemperature between 24–26 C dan pH 8. 
Isolasi dan Identifikasi Bakteri Gram Negatif Pada Luka Ikan Maskoki (Carassius auratus) Akibat Infestasi Ektoparasit Argulus sp. [Isolation and Identification Gram Negative Bacteria At Lessions Of Gold Fish (Carassius auratus) By Infestation Ectoparasite Argulus sp. Kismiyati Kismiyati; Sri Subekti; R. Wahid Nur Yusuf; Rahayu Kusdarwati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 2 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i2.11678

Abstract

AbstractOne of them is the problem of disease Argulus sp. is ektoparasite which often attacked in activity of good conducting of sea water and also freshwater. Argulus sp. attacking at part of fin, husk, gill and entire all its body exterior of him. attacked by fish is Argulus sp. will seen is thin, often rub body and arise red pock which later can generate secondary infection. Utilize to prove the existence of bacterium which emerge effect of lession (secondary infection) which because of bite of Argulus sp. that is by identifying bacterium and biochemical test. Media which used in identifying bacterium for example that is TSA (Tryptic Soy Agar), TSIA (Triple Soy Iron Agar), MIO (Motility Indol Ornithin), O/F medium (Oksidative/Fermentative), sugar test medium (maltosa, laktosa, arabinosa, inositol, manitol, sukrosa). Target of this research is to know the existence of gram negative bacterium and gram negative bacterium any kind of which is found at fish hurt of gold fish (Carassius auratus) effect of infestation by ectoparasite Argulus sp. Research which is use descriptive method. That is with infestation of Argulus sp. into containing aquarium of gold fish counted 1 tail and Argulus sp. counted 5, 10, 15, 20 tail. After Argulus sp. patch all at gold fish is later then perceived until gold fish arise lession effect of bite of Argulus sp. is afterwards identifyed by bacterium to know the bacterium that were formed on gold fish lesions. The result showed that the lesions of gold fish, isolation at TSA medium and identified by biochemical test to diagnose bacteriums found on gold fish lession. Identifications of the three gram negative bacterium as follow : Aeromonas hydrophilla, Pseudomonas flourescens, Flexibacter columnaris while for the Edwardsiella tarda was not be found in this study
The Toxic Effect of Ametryn on Behaviour and Histopathological Changes of Freshwater Eel (Monopterus Albus) [Pengaruh Toksik Ametryn terhadap Perilaku dan Perubahan Histopatologis Belut AirTawar (Monopterus Albus)] Marina Hasan; Nor Azri Shah Norhan; Wahidah Wahab; Anur Abdalah Nagi Melad; Kismiyati Kismiyati; Mohd Fazrul Hisam Abd Aziz; Norainy Mohd Husin; Mohd Ihwan Zakariah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 11 No. 2 (2019): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v11i2.15713

Abstract

AbstractA study on the toxicity of herbicides was investigated with emphasis on behaviour and histopathological effects. Ametryn commonly used to control weeds in the paddy field. The effect of ametryn on fish especially Monopterus albus was unknown. This study aims to determine the acute toxicity of ametryn and its effects on the behaviour of the eels. The adult M. albus were exposed to 0, 0.75, 1.5 and 3.0mg/L of ametryn for 96 hours under laboratory conditions. The eels showed symptoms of abnormal behaviour responses during the exposure which are agitated behaviour, respiratory distress, and abnormal nervous behaviour. Mortality was increased by increasing the concentration of ametryn. This present study showed the effect of ametryn on kidney tissue changes. Hemorrhage and distribution of hemosiderin, hydropic degeneration, granular degeneration, tubular necrosis, narrowing tubular lumen and necrosis were major histopathological effects that occurred during the exposure to ametryn. In conclusion, ametryn is very sensitive to Monopterus albus. In lower concentration, they showed changes in behaviour and effect their organs. AbstrakStudi tentang toksisitas herbisida yang diamati dengan penekanan pada perilaku dan dampak histopatologis. Ametryn biasanya digunakan untuk mengawal rumpai di sawah padi. Kesan ametryn pada ikan terutama belut tidak diketahui. Penelitian ini bertujuan untuk menentukan toksisitas akut ametryn dan dampaknya terhadap perilaku belut. M. albus dewasa terpapar pada 0, 0.75, 1,5 dan 3,0mg / L ametryn selama 96 jam pada kondisi laboratorium. Belut menunjukkan gejala respons perilaku abnormal selama paparan yaitu gelisah, gangguan pernapasan, dan perilaku saraf abnormal. Kematian meningkat seiring meningkatnya konsentrasi ametryn. Penelitian ini menunjukkan dampak ametryn pada perubahan jaringan ginjal. Perdarahan dan distribusi hemosiderin, degenerasi hidropik, degenerasi granular, nekrosis tubular, penyempitan lumen tubular dan nekrosis adalah dampak histopatologis utama yang terjadi selama paparan ametryn. Kesimpulannya, ametryn sangat sensitif terhadap Monopterus albus. Dalam konsentrasi rendah, belut menunjukkan perubahan perilaku dan kesan pada organ.
MORPHOLOGICAL COMPARISON OF Octolasmis sp. ECTOPARASITES INFESTING THE GILLS OF SCALLOPED SPINY LOBSTER (Panulirus homarus) WITH DIFFERENT FIXATIVE SOLUTIONS Yusuf Taufik Hidayat; Kismiyati; Muhamad Amin
Jurnal Biosains Pascasarjana Vol. 25 No. 2 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i2.2023.156-162

Abstract

Octolasmis is a type of ectoparasite which is often found on the gills of lobsters. Octolasmis can also be a threat to lobster populations and can even cause high mortality rates. This study aims to detect the effect of different fixative solutions on the morphological characters of Octolasmis infesting the gills of scalloped spiny lobsters (Panulirus homarus). Samples of Octolasmis were collected from the gills of wildly-caught scalloped spiny lobsters in East Lombok, West Nusa Tenggara. The samples were immersed separately in two different fixative solution which are either absolute ethanol or formalin solution. After 7 days, morphological analysis of Octolasmis was analysed using an integrated optilab stereo binocular microscope and a lucida camera. The result showed that there were significant differences in the morphological condition of Octolasmis sp. including skin colour, organ appearance and size of the Octolasmis. In general, the result showed that formalin is better fixative solution.
IDENTIFICATION OF Anisakis simplex IN PURPLE SPOTTED BIG EYE FISH (Priacanthus sp.) FROM SOUTH BALI WATER AREA Amayliana Ajeng Nastiti; Sri Subekti; Kismiyati Kismiyati; Endang Wulandari Suryaningtyas
Jurnal Perikanan Unram Vol 14 No 4 (2024): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v14i4.1300

Abstract

Anisakis simplex infection in fishery products can be dangerous because it has zoonotic can transmitted infection from fish to humans. Purple spotted big eye fish (Priacanthus sp.) is one of the reef fish species produced in the waters of South Bali and is not immune to the zoonotic threat Anisakis simplex poses. This study was conducted to identify Anisakis simplex which is known to infect Purple Spotted Big Eye Fish (Priacanthus sp.) in the South Bali Water Area. The parameters observed in this study were related to the prevalence and intensity of Anisakis simplex in Purple Spotted Big Eye Fish (Priacanthus sp.). The research method used was descriptive quantitative with purposive sampling of fish samples. The samples used were 30 fish obtained from 15-20% of the catch of purple spotted big eye fish in the South Bali Water Area. The results of the study showed that the morphological identification that had been carried out showed that Anisakis simplex found to infect Purple Spotted Big Eye Fish (Priacanthus sp.) was an Anisakis simplex type III larva with a prevalence rate of 26.3% classified as a frequent infection. The intensity value of Anisakis simplex in this study was 4.92 ind/fish and was classified as a low category. The predilection from this study showed that the most infections were in the intestinal organs.
Co-Authors A. Shofy Mubarak Ade Fitri Noor Inaya Akhmad Taufiq Mukti Amayliana Ajeng Nastiti Annisa Novia Damayanti Anur Abdalah Nagi Melad Ardilas Heryamin Arika Juniarsih Aulia Ikhsan Syamsuri Auriga Putri Faradilla Benedikta Tania Diklauia Berliana A Bernideta Dewi Kriswijayanti DARMAWAN SETIA BUDI Devy Agustia Pratiwi Dewi, Nina Nurmalia Diah Ayu Puspitarini dika dika Endang Wulandari Suryaningtyas Fahdi Putra Utama Faisol Mas&#039;ud Fatih Riantono Febri Setyawati Ferry Dwi Firmansyah Liananda Gita Zahrani Aryandini Gunanti Mahasri Hari Suprapto Ikmalia A Iqbal Ghazali Kusnoto Kusnoto Laksmi Sulmartiwi Linda Megawati Yanuaris Luthfiana Aprilianita Sari Lyla Wulandari M Wahyu Alfian Marina Hasan Marina hasan Mirni Lamid Mohammad Faizal Ulkhaq Mohd Fazrul Hisam Abd Aziz Mohd Fazrul Hisam Abd Aziz Mohd Ihwan Zakariah Mohd Ihwan Zakariah Muhamad Amin Muhammad Aiman Yudarana Yudarana Muhammad Arief Muhammad Yusuf Nadia Ayu Fadila Asshaufi Naufal Falatehan Nor Azri Shah Norhan Norainy Mohd Husin Norma Isnawati Novy Prasetya Nur Maulana Iskhaq P Renita Efa Ratna Sari Puput Puspitasari R. Wahid Nur Yusuf Rahayu Kusdarwati Rakhmarani Nur Fatiza Rengga Eko Pradipta Renita Efa Ratna Sari Riza Aryani Rozi Rozi Rozi Rozi Rr. Juni Triastuti Santanumurti, Muhammad Browijoyo Sari, Putri Desi Wulan Selvi Oktora Mahanani Setiawan Koesdarto Setyawati, Febri Sri Subekti Sri Subekti Sudarno, Sudarno Sulastri Arsad Tjahjaningsih, Wahju Vivaldy Phaza Muharta Wahidah Wahab Woro Hastuti Setyantini Yogita Tirta Noviyanti Yudha Teguh Prayogi Yudin Ardha Pradipta Yustika Rahmawati Yusuf Taufik Hidayat Zurica Melati Fitri