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Isolasi dan Identifikasi Bakteri Gram Negatif Pada Luka Ikan Maskoki (Carassius auratus) Akibat Infestasi Ektoparasit Argulus sp. [Isolation and Identification Gram Negative Bacteria At Lessions Of Gold Fish (Carassius auratus) By Infestation Ectoparasite Argulus sp. Kismiyati Kismiyati; Sri Subekti; R. Wahid Nur Yusuf; Rahayu Kusdarwati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 2 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i2.11678

Abstract

AbstractOne of them is the problem of disease Argulus sp. is ektoparasite which often attacked in activity of good conducting of sea water and also freshwater. Argulus sp. attacking at part of fin, husk, gill and entire all its body exterior of him. attacked by fish is Argulus sp. will seen is thin, often rub body and arise red pock which later can generate secondary infection. Utilize to prove the existence of bacterium which emerge effect of lession (secondary infection) which because of bite of Argulus sp. that is by identifying bacterium and biochemical test. Media which used in identifying bacterium for example that is TSA (Tryptic Soy Agar), TSIA (Triple Soy Iron Agar), MIO (Motility Indol Ornithin), O/F medium (Oksidative/Fermentative), sugar test medium (maltosa, laktosa, arabinosa, inositol, manitol, sukrosa). Target of this research is to know the existence of gram negative bacterium and gram negative bacterium any kind of which is found at fish hurt of gold fish (Carassius auratus) effect of infestation by ectoparasite Argulus sp. Research which is use descriptive method. That is with infestation of Argulus sp. into containing aquarium of gold fish counted 1 tail and Argulus sp. counted 5, 10, 15, 20 tail. After Argulus sp. patch all at gold fish is later then perceived until gold fish arise lession effect of bite of Argulus sp. is afterwards identifyed by bacterium to know the bacterium that were formed on gold fish lesions. The result showed that the lesions of gold fish, isolation at TSA medium and identified by biochemical test to diagnose bacteriums found on gold fish lession. Identifications of the three gram negative bacterium as follow : Aeromonas hydrophilla, Pseudomonas flourescens, Flexibacter columnaris while for the Edwardsiella tarda was not be found in this study
The Toxic Effect of Ametryn on Behaviour and Histopathological Changes of Freshwater Eel (Monopterus Albus) [Pengaruh Toksik Ametryn terhadap Perilaku dan Perubahan Histopatologis Belut AirTawar (Monopterus Albus)] Marina Hasan; Nor Azri Shah Norhan; Wahidah Wahab; Anur Abdalah Nagi Melad; Kismiyati Kismiyati; Mohd Fazrul Hisam Abd Aziz; Norainy Mohd Husin; Mohd Ihwan Zakariah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 11 No. 2 (2019): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v11i2.15713

Abstract

AbstractA study on the toxicity of herbicides was investigated with emphasis on behaviour and histopathological effects. Ametryn commonly used to control weeds in the paddy field. The effect of ametryn on fish especially Monopterus albus was unknown. This study aims to determine the acute toxicity of ametryn and its effects on the behaviour of the eels. The adult M. albus were exposed to 0, 0.75, 1.5 and 3.0mg/L of ametryn for 96 hours under laboratory conditions. The eels showed symptoms of abnormal behaviour responses during the exposure which are agitated behaviour, respiratory distress, and abnormal nervous behaviour. Mortality was increased by increasing the concentration of ametryn. This present study showed the effect of ametryn on kidney tissue changes. Hemorrhage and distribution of hemosiderin, hydropic degeneration, granular degeneration, tubular necrosis, narrowing tubular lumen and necrosis were major histopathological effects that occurred during the exposure to ametryn. In conclusion, ametryn is very sensitive to Monopterus albus. In lower concentration, they showed changes in behaviour and effect their organs. AbstrakStudi tentang toksisitas herbisida yang diamati dengan penekanan pada perilaku dan dampak histopatologis. Ametryn biasanya digunakan untuk mengawal rumpai di sawah padi. Kesan ametryn pada ikan terutama belut tidak diketahui. Penelitian ini bertujuan untuk menentukan toksisitas akut ametryn dan dampaknya terhadap perilaku belut. M. albus dewasa terpapar pada 0, 0.75, 1,5 dan 3,0mg / L ametryn selama 96 jam pada kondisi laboratorium. Belut menunjukkan gejala respons perilaku abnormal selama paparan yaitu gelisah, gangguan pernapasan, dan perilaku saraf abnormal. Kematian meningkat seiring meningkatnya konsentrasi ametryn. Penelitian ini menunjukkan dampak ametryn pada perubahan jaringan ginjal. Perdarahan dan distribusi hemosiderin, degenerasi hidropik, degenerasi granular, nekrosis tubular, penyempitan lumen tubular dan nekrosis adalah dampak histopatologis utama yang terjadi selama paparan ametryn. Kesimpulannya, ametryn sangat sensitif terhadap Monopterus albus. Dalam konsentrasi rendah, belut menunjukkan perubahan perilaku dan kesan pada organ.
MORPHOLOGICAL COMPARISON OF Octolasmis sp. ECTOPARASITES INFESTING THE GILLS OF SCALLOPED SPINY LOBSTER (Panulirus homarus) WITH DIFFERENT FIXATIVE SOLUTIONS Yusuf Taufik Hidayat; Kismiyati; Muhamad Amin
Jurnal Biosains Pascasarjana Vol. 25 No. 2 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i2.2023.156-162

Abstract

Octolasmis is a type of ectoparasite which is often found on the gills of lobsters. Octolasmis can also be a threat to lobster populations and can even cause high mortality rates. This study aims to detect the effect of different fixative solutions on the morphological characters of Octolasmis infesting the gills of scalloped spiny lobsters (Panulirus homarus). Samples of Octolasmis were collected from the gills of wildly-caught scalloped spiny lobsters in East Lombok, West Nusa Tenggara. The samples were immersed separately in two different fixative solution which are either absolute ethanol or formalin solution. After 7 days, morphological analysis of Octolasmis was analysed using an integrated optilab stereo binocular microscope and a lucida camera. The result showed that there were significant differences in the morphological condition of Octolasmis sp. including skin colour, organ appearance and size of the Octolasmis. In general, the result showed that formalin is better fixative solution.
IDENTIFICATION OF Anisakis simplex IN PURPLE SPOTTED BIG EYE FISH (Priacanthus sp.) FROM SOUTH BALI WATER AREA Amayliana Ajeng Nastiti; Sri Subekti; Kismiyati Kismiyati; Endang Wulandari Suryaningtyas
Jurnal Perikanan Unram Vol 14 No 4 (2024): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v14i4.1300

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Anisakis simplex infection in fishery products can be dangerous because it has zoonotic can transmitted infection from fish to humans. Purple spotted big eye fish (Priacanthus sp.) is one of the reef fish species produced in the waters of South Bali and is not immune to the zoonotic threat Anisakis simplex poses. This study was conducted to identify Anisakis simplex which is known to infect Purple Spotted Big Eye Fish (Priacanthus sp.) in the South Bali Water Area. The parameters observed in this study were related to the prevalence and intensity of Anisakis simplex in Purple Spotted Big Eye Fish (Priacanthus sp.). The research method used was descriptive quantitative with purposive sampling of fish samples. The samples used were 30 fish obtained from 15-20% of the catch of purple spotted big eye fish in the South Bali Water Area. The results of the study showed that the morphological identification that had been carried out showed that Anisakis simplex found to infect Purple Spotted Big Eye Fish (Priacanthus sp.) was an Anisakis simplex type III larva with a prevalence rate of 26.3% classified as a frequent infection. The intensity value of Anisakis simplex in this study was 4.92 ind/fish and was classified as a low category. The predilection from this study showed that the most infections were in the intestinal organs.
Microbial Profile of Frozen Fisheries Products at UPT PMP2KP Surabaya Fadiya Furuujihim Rohsarifuddin; Yolandha Sephiani Nurhafifah; Puspa Maharani; Kismiyati Kismiyati; Rahmah Hendriyati; Woro Hastuti Satyantini
Juvenil Vol 6, No 1: Februari (2025)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v6i1.26337

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ABSTRACTMicrobial contamination of food products is one of the significant hazards in food safety because the nature of food-polluting bacteria is generally pathogenic.This research was conducted at the Microbiology Laboratorium in UPT PMP2KP Surabaya. Samples tested in this research including frozen fish, frozen shrimp, and frozen value added products with  microbiological parameters tested are Total Plate Count, Salmonella, and Escherichia coli. The results obtained in this research showed that 100% of 21 frozen fish and shrimp samples met SNI safety standards, namely 1 x 105  CFU/g for TPC, 3 MPN / g  for E. coli, and negative for Salmonella.Keywords: E. coli, Food Safety, Frozen Fisheries Product, Salmonella
Periodic Fasting Treatment on Growth Performance and Feed Efficiency of Nile Tilapia (Oreochromis niloticus, GIFT) Jacob Da Costa Pereira; Salustiano Zaret; Mateus Salvador; Kismiyati; Sapto Adriyono; Ahmad Shofy Mubarak; Mohammad Anam Al Arif
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.81125

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Fasting is a feed management strategy that reduces feeding frequency without inhibiting fish growth. This strategy involves specific intervals of feed deprivation, allowing fish to exhibit increased appetite once feeding resumes. This study aimed to evaluate the effects of periodic fasting on the growth performance and feed efficiency of GIFT Nile tilapia, as well as to identify the most effective fasting pattern for optimal results. The research used a completely randomized design (CRD) with four treatments, each replicated three times: continuous feeding without fasting (K, control); two days of feeding followed by one day of fasting (2E1F); three days of feeding followed by one day of fasting (3E1F); and four days of feeding followed by one day of fasting (4E1F). The primary parameters observed were absolute weight gain (g), absolute length growth (cm), and feed efficiency. Data were analyzed using the Kruskal–Wallis test (p < 0.05), followed by post hoc comparisons using independent samples tests to determine significant differences among treatments. The results showed that periodic fasting had a significant effect (p < 0.05) on absolute weight gain, absolute length growth, and feed efficiency of GIFT Nile tilapia. The best performance was observed in treatment 4E1F (four days of feeding followed by one day of fasting), while the lowest performance occurred in treatment 2E1F (two days of feeding followed by one day of fasting).
Inventarisation of Protozoan and Arthropoda Ectoparasitic Infestation in Freshwater Fish in Banyuwangi City, East Java Mohammad Faizal Ulkhaq; Darmawan Setia Budi; Kismiyati ,
Journal of Aquaculture Science Vol 3 No 1 (2018): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v3i1.36

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Detriment caused by ectoparasitic infestation was not as big detriment caused by infestation of other organisms such as viruses and bacteria, but it could be a predisposing factor for the infection of more dangerous pathogenic in fish. The aims of this study was to inventory ectoparasites that infecting freshwater fish cultivated in several districts in the Banyuwangi, in which the results were expected to be used as a reference in an effort to prevent the spread of disease in fish culture. The sample of research consisted of tilapia fish, carp and catfish which were obtained from six sub districts in Banyuwangi, those were Kota, Kabat, Licin, Kalipuro, Giri, and Glagah. The ectoparasite examination method was performed naturally by scraping the surface of the body, fins and gills of the fish, then observed under a microscope with magnification ranging from 40 to 400x. The results of further observations were documented and identified by key identification. Ectoparasites which were found infesting freshwater fish in the city of Banyuwangi consisted of 4 genuses: namely Argulus, Lernaea, Chonopeltis and Trichodina. Further research is needed to determine the prevalence and intensity of ectoparasites in order to prevent the spread of ectoparasites in other regions.   Keywords: Inventarisation, ectoparasites, freshwater fish, Banyuwangi
Analisis Prevalensi dan Intensitas Neobenedenia girellae Pada Ikan Kakap Putih (Lates calcarifer) di Perairan Lampung dan Situbondo Muhammad Aiman Yudarana Yudarana; Sri Subekti; Kismiyati
Journal of Aquaculture Science Vol 7 No 2 (2022): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v7i2.240

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Barramundi (Lates calcarifer) is a fish that has high economic value and has the potential to be developed in Indonesia. One of the species reported to barramundi is infest Neobenedenia girellae. This study aims to determine the prevalence and intensity of the parasitic worm Neobenedenia girellae in barramundi in the waters of Lampung and Situbondo. Sampling of barramundi with a size of 21 - 30 cm as many as 40 fish from Lampung waters and 50 barramundi from Situbondo waters. The results showed that there was worm infestation Neobenedenia girellae on barramundi in the waters of Lampung and Situbondo. The prevalence value of Neobenedenia girellae in barramundi in Lampung waters is 92% with almost always category and intensity value of 1.37 with low category when in Situbondo waters the prevalence value is 92% with almost always category and the intensity value is 1.67 with low category. Keywords: Lates calcarifer, Intensity, Prevalence, Neobenedenia girellae
Sex-Based Morphological Affecting Post-Detachment Survival of Argulus ja-ponicus from Common Carp (Cyprinus carpio) Gilang Septian Gilang; Kismiyati; Marina Hasan
Journal of Aquaculture Science Vol 11 No 1 (2026): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joas.v11i1.77216

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This study looks at how differences in body shape and behavior between male and female Argulus japonicus influence their survival after separating from their host. A total of 200 specimens (100 males and 100 females) were collected from infected goldfish (Cyprinus carpio) in Sidoarjo, East Java. Each parasite was then observed individually in aerated petri dishes at a consistent room temperature of 28 °C. Microscopic observations showed that males tend to have a more elongated and slender body, while females are generally shorter and rounder. This difference is likely related to reproductive needs, especially egg production in females. The results also indicate a clear difference in survival between the sexes. Females frequently detach from the host to lay eggs, a process that appears to cause stress and may reduce their lifespan. On the other hand, males are more likely to remain attached to the host, which may help them maintain a more stable condition and live longer. Overall, these findings suggest that reproductive behavior plays an important role in determining how long these parasites can survive outside their host. This information could be useful for developing more effective control strategies in aquaculture
Co-Authors A. Shofy Mubarak Ade Fitri Noor Inaya Akhmad Taufiq Mukti Al arif, Mohammad Anam Amayliana Ajeng Nastiti Annisa Novia Damayanti Anur Abdalah Nagi Melad Ardilas Heryamin Arika Juniarsih Aulia Ikhsan Syamsuri Auriga Putri Faradilla Benedikta Tania Diklauia Berliana A Bernideta Dewi Kriswijayanti DARMAWAN SETIA BUDI Devy Agustia Pratiwi Diah Ayu Puspitarini dika dika Endang Wulandari Suryaningtyas Fadiya Furuujihim Rohsarifuddin Fahdi Putra Utama Faisol Mas&#039;ud Fatih Riantono Febri Setyawati Ferry Dwi Firmansyah Liananda Gilang Septian Gilang Gita Zahrani Aryandini Gunanti Mahasri Hari Suprapto Ikmalia A Iqbal Ghazali Jacob Da Costa Pereira Kusnoto Kusnoto Laksmi Sulmartiwi Linda Megawati Yanuaris Luthfiana Aprilianita Sari Lyla Wulandari M Wahyu Alfian Marina Hasan Marina hasan Marina Hasan Mateus Salvador Mirni Lamid Mohammad Faizal Ulkhaq Mohd Fazrul Hisam Abd Aziz Mohd Fazrul Hisam Abd Aziz Mohd Ihwan Zakariah Mohd Ihwan Zakariah Muhamad Amin Muhammad Aiman Yudarana Yudarana Muhammad Arief Muhammad Yusuf Nadia Ayu Fadila Asshaufi Naufal Falatehan Nina Nurmalia Dewi Nor Azri Shah Norhan Norainy Mohd Husin Norma Isnawati Novy Prasetya Nur Maulana Iskhaq P Renita Efa Ratna Sari Puput Puspitasari Puspa Maharani R. Wahid Nur Yusuf Rahayu Kusdarwati Rahmah Hendriyati Rakhmarani Nur Fatiza Rengga Eko Pradipta Renita Efa Ratna Sari Riza Aryani Rozi Rozi Rr. Juni Triastuti Salustiano Zaret Santanumurti, Muhammad Browijoyo Sapto Adriyono Sari, Putri Desi Wulan Selvi Oktora Mahanani Setiawan Koesdarto Setyantini, Woro Hastuti Setyawati, Febri Sri Subekti Sri Subekti Sudarno, Sudarno Sulastri Arsad Tjahjaningsih, Wahju Vivaldy Phaza Muharta Wahidah Wahab Woro Hastuti Satyantini Yogita Tirta Noviyanti Yolandha Sephiani Nurhafifah Yudha Teguh Prayogi Yudin Ardha Pradipta Yustika Rahmawati Yusuf Taufik Hidayat Zurica Melati Fitri