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All Journal Jurnal Ilmu Dasar Jurnal Ilmu Kedokteran (Journal of Medical Science) Majalah Kedokteran Bandung AL KAUNIYAH Journal of Agromedicine and Medical Sciences Biology, Medicine, & Natural Product Chemistry Elkawnie MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Syntax Literate: Jurnal Ilmiah Indonesia Medika Kartika : Jurnal Kedokteran dan Kesehatan JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jember Medical Journal (JMJ) International Journal of Health and Information System (IJHIS)
Sheilla Rachmania, Sheilla
Department Of Histology, Faculty Of Medicine, University Of Jember, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Analysis of Microglia Morphology and Number in Wistar Rats Brain After CIDR1α-PfEMP1 Recombinant Protein Injection Sulistyaningsih, Erma; Putri, Izza Amalia; Rachmania, Sheilla
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.38242

Abstract

AbstractOne malaria vaccine candidate is Cysteine-rich Interdomain Region 1α (CIDR1α) of Plasmodium falciparumErythrocyte Membrane Protein 1 (PfEMP1), an essential protein involved in the pathogenesis of cerebral malaria. Microglia in the brain act as the first line of defense against brain pathological changes. The study aimed to evaluate the response of brain microglia to the CIDR1α-PfEMP1 recombinant protein injection by observing microglia morphology and number in rat’s cerebral cortex. 12 Wistar rats were divided into the control group, which was injected with normal saline solution, and the treatment group, which was injected with 150 µg CIDR1α-PfEMP1 recombinant protein combined with adjuvants. Injection was conducted thrice within three-week intervals (day 1, 21, and 42). Wistar rats were euthanized on day 56, and histological slides were prepared with Hematoxylin-Eosin staining. Examination using a microscope, 400x, and Fiji Image J software showed microglia morphology of ramified and rod cells in both the control and treatment groups. The microglia number in the control group was 93.00 ± 5.77, and the treatment group was 105.75 ± 15.62. Statistical analysis using an independent t-test showed no significant differences between groups (p= 0.15). The result indicated that the injection of CIDR1α-PfEMP1 recombinant protein did not provoke pathological changes in brain tissue, which induced a microglia response. This study strengthens the potential of the CIDR1α-PfEMP1 recombinant protein as a peptide-based malaria vaccine candidate.AbstrakSalah satu kandidat vaksin malaria adalah Cysteine-rich Interdomain Region 1α (CIDR1α) dari Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1), protein penting dalam patogenesis malaria serebral. Mikroglia di otak berperan sebagai pertahanan lini pertama terhadap perubahan di otak. Penelitian ini bertujuan mengevaluasi respon mikroglia otak terhadap pemberian protein rekombinan CIDR1α-PfEMP1 dengan mengamati morfologi dan jumlah mikrolia pada korteks serebri otak tikus. 12 tikus Wistar dibagi dalam kelompok kontrol yang diinjeksi normal saline dan  kelompok perlakuan diinjeksi 150 µg protein rekombinan CIDR1α-PfEMP1 yang dikombinasikan dengan adjuvant. Injeksi dilakukan tiga kali dengan interval tiga minggu (hari 1, 21, dan 42). Tikus dieuthanasia pada hari ke-56 dan preparat histologi otak disiapkan dengan pengecatan Hematoxyline-Eosin. Pengamatan menggunakan mikroskop 400x dan Fiji Image J software menunjukkan morfologi ramified dan rod cell pada kelompok kontrol maupun perlakuan. Jumlah mikroglia pada kelompok kontrol 93,00 ± 5,7 sedangkan kelompok perlakuan 105,75 ± 15,62). Analisis statistik menggunakan independent-t test menunjukkan tidak terdapat perbedaan yang bermakna antara 2 kelompok (p= 0,15). Hasil ini mengindikasikan bahwa pemberian protein rekombinan CIDR1α-PfEMP1 tidak menimbulkan patologi pada jaringan otak yang memicu respon mikroglia. Hal ini menguatkan potensi protein rekombinan CIDR1α-PfEMP1 sebagai kandidat vaksin malaria berbasis peptida.
PROFIL HEMODINAMIK PASIEN PRE OPERATIF, INTRA OPERATIF, DAN PASCA OPERATIF DENGAN HIPOALBUMIN Gemawan, Taufiq; Handoko, Adelia; Rachmania, Sheilla; Hasya, Aghnia; Aisy, Rihadatul; Pratama, Muhamad Rizal Hadi
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 7 No 4 (2024): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Abstract

Albumin serum (SA) dibutuhkan dalam tubuh sebagai protein yang multifungsi. Peran penting albumin adalah untuk menekan respons inflamasi dalam tubuh dan menjaga permeabilitas vaskuler terutama saat proses inflamasi. Hal ini menjadi perhatian penting bagi pasien yang akan melakukan tindakan operatif untuk menjaga hemodinamik vaskuler. Berikut ini adalah serial kasus mengenai dua pasien dengan diagnosis canal stenosis lumbal dan hernia sikatrikalis. Hasil laboratorium albumin kedua pasien tersebut adalah <3,5 g/dL. Kedua pasien tersebut dipantau kondisi hemodinamik praoperatif, intraoperatif, dan pascaoperatif di Rumah Sakit Daerah dr. Soebandi, Jember, Indonesia. Semua pasien dengan hipoalbuminemia pada penelitian ini mengalami kondisi komorbid hipertensi. Kondisi hemodinamik praoperatif dan pascaoperatif relatif sama, sedangkan saat intraoperatif tekanan darah pasien mengalami penurunan. Albumin memiliki peran dalam mempertahankan permeabilitas vaskuler. Pemeriksaan albumin pada pasien praoperatif penting dilakukan untuk menimbang apakah hemodinamik intraoperatif dan pascaoperatif pasien dapat stabil. Kata Kunci: heart rate, hipoalbumin, mean arterial pressure (MAP), tekanan darah DOI : 10.35990/mk.v7n4.p432-441
Potential of shallot peels as a daily antioxidant supplement against cigarette smoke-induced lung damage Helianti, Dina; Dewi, Rosita; Munawaroh, Ayu; Rachmania, Sheilla; Maulana, Aditha Satria; Abrori, Cholis; Sumadi
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 3, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss3.art10

Abstract

Background: Cigarette smoking damages the alveoli through oxidative stress. Shallot peels containing flavonoids, especially quercetin, potentially serve as a daily antioxidant supplement to impede lung tissue damage induced by cigarette smoke. However, the maximum effective dose is yet to be determined. Objective: This research was designed to establish the maximum effective dose of shallot peel infusion (SPI) to prevent oxidative stress and histopathological lung damage induced by cigarette smoke. Methods: This experimental laboratory was a posttest-only control group design. A total of 24 male Rattus norvegicus Wistar strain, were allocated into 6 groups: a control group and 5 SPI-treated groups. All rats were exposed to 2 cigarettes/day and were treated for 28 days with aquabidest and different doses of SPI (0 mg/kgBW, 125 mg/kgBW, 250 mg/kgBW, 500 mg/kgBW, 1,000 mg/kgBW, and 2,000 mg/kgBW). The level of oxidative stress in serum was measured malondialdehyde (MDA) level with ELISA, and histopathological lung damage was estimated using the lung histopathological damage scoring method assessing inflammatory cells, alveolus lumen and inter-alveoli junction.Results: The quadratic regression analysis revealed the maximum effective dose of SPI to prevent oxidative stress and lung damage were 1,435 mg/kgBW and 1,206 mg/kgBW, respectively. In the histopathological examination of the lungs, the administration of SPI up to a dose of 1206 mg/kg BW prevents the inflammatory process caused by cigarette smoke, which is indicated by the number of inflammatory cells, the thickness of the alveolar septum, and the increasingly normal shape of the alveolar lumen.Conclusion: SPI doses of less than 1,206 mg/kgBW are safe and effective daily antioxidant supplements in rats exposed to cigarette smoke and have the potential to be further studied for application in humans.
Liver Histopathological Analysis in The Acute Toxicity Test of Shallot (Allium cepa L.) Peel Extract in Rats (Rattus norvegicus) Aprilya Pratiwi, Nadilla; Dewi, Rosita; Agustina, Dini; Helianti, Dina; Rachmania, Sheilla
Journal of Agromedicine and Medical Sciences Vol. 11 No. 3 (2025)
Publisher : Faculty of Medicine, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v11i3.53736

Abstract

Shallot (Allium cepa L.) contains bioactive compounds with antioxidant effects. In addition to the tuber, the shallot’s peel is a rich source of flavonoids with demonstrated capacity to mitigate oxidative stress. Prior studies have evaluated the antioxidant efficacy of shallot peel in ameliorating organ damage and have established its maximum effective dose. The subsequent step required for its potential therapeutic application is toxicity test. This study aimed to analyze the liver histopathological changes in the acute toxicity test of shallot peel extract (SPE) in rats based on OECD TG 420. This research was an experimental with a post-test-only control group design. Five female rats were used as the control group administered dimethyl sulfoxide (DMSO), while 5 female rats were used in the treatment group administered SPE at 5,000 mg/kg body weight (BW) (one of them had been used for a preliminary test with the same dosage). On day 15, a necropsy was conducted, followed by histopathological observation of the hematoxylin-eosin (HE)-stained liver histopathological slide. The damage to hepatocytes was evaluated using Manja Roenigk criteria. The average histopathological score per liver cell of the control group was 1.226 ± 0.0065 and the treatment group was 1.235±0.0079. The Mann-Whitney test showed that the liver histopathological score of the treatment group was not different from that of the control group (p>0.05). It can be concluded that SPE 5,000 mg/kg does not show acute toxic effects in rats, with LD₅₀ estimated at >5,000 mg/kg according to OECD standards.
Co-Authors Aisy, Rihadatul Alami, Eqiel Navadz Akthar Ancah Caesarina Novi Marchianti Andriani, Made Prasanti Angga Mardro Raharjo, Angga Mardro Aprilya Pratiwi, Nadilla Arsyzilma Hakiim Ayu Munawaroh Aziz, Ayu Munawaroh Bachtiar Hidayat, Aldy Bintang, Muhammad Farrel Ravidinata Masoga Caesarina, Ancah Cholis Abrori, Cholis Cicih Komariah Desie Dwi Wisudanti Dina Helianti Dini Agustina Dwisaraswati, Salma Aulia Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Erfan Efendi Erma Sulistyaningsih Erma Sulistyaningsih Erma Sulistyaningsih Gianevan, Nicholas Jirezra Handoko, Adelia Hasya, Aghnia Hibatullah, Muhammad Naufal Ida Sri Surani Wiji Astuti Ida Srisurani Wiji Astuti Inke Kusumastuti Irawan Fajar Kusuma Irawan Fajar Kusuma Izza Amalia Putri Jauhar Firdaus Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Lusi Padma Sulistianingsih Mata Luthfiyyah, Nur Atikah M. Ali Shodikin Maulana, Aditha Satria Muhammad Afiful Jauhani Muhammad Hasan Mukhammad Arif Hadi Khoiruddin Munawaroh, Ayu Nizar Fiska Bayu Agustian Nofica, Cheila Nurmaida, Eny Pratama, Muhamad Rizal Hadi Putri, Elvia Rahmi Marga Putri, Izza Amalia Rahma, Nafisah Hani Asyifah Rena Normasari Rini Riyanti Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rumastika, Nindya Shinta Sahrir Sillehu Sa’diyah, Nindya Audatus septa surya wahyudi, septa surya Sudarmanto, Yohanes Sulistyaningsih , Erma Sumadi Supangat Supangat Suryono Suryono Taufiq Gemawan Yunita Armiyanti Zahniar, Zahniar