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All Journal Jurnal Ilmu Dasar Jurnal Ilmu Kedokteran (Journal of Medical Science) Majalah Kedokteran Bandung AL KAUNIYAH Journal of Agromedicine and Medical Sciences Biology, Medicine, & Natural Product Chemistry Elkawnie MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Syntax Literate: Jurnal Ilmiah Indonesia JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jember Medical Journal (JMJ) International Journal of Health and Information System (IJHIS)
Sheilla Rachmania, Sheilla
Department Of Histology, Faculty Of Medicine, University Of Jember, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Molecular Method Optimization to Identify Plasmodium falciparum Multidrug Resistance 1 (pfmdr1) gene as a Predictor of Antimalarial Resistance Sulistyaningsih, Erma; Dewi, Rosita; Rachmania, Sheilla; Kusuma, Irawan Fajar; Kholifaturrohmy, Muhammad Rizqi; Armiyanti, Yunita; Kholifaturrohmah, Sakinatus Sariroh; Andriani, Made Prasanti
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.673-678

Abstract

Several approaches have been designed to control malaria, a disease with high morbidity and mortality, but they face some hurdles. Antimalarial resistance is one of the major challenges for malaria elimination, so the detection of antimalarial resistance is essential. Several molecular markers for antimalarial resistance have been identified, including Plasmodium falciparum multidrug resistance 1 (pfmdr1) gene. This study determined the optimization of molecular techniques to identify the pfmdr1 gene as an antimalarial resistance predictor in Indonesia. The study included patients diagnosed with uncomplicated or severe malaria originating from the health district of Kerom Regency, Papua Province, and Dr. Soebandi Hospital, Jember, East Java Province. Blood samples were collected in the Whatmann filer paper after informed consent. DNA was isolated from dried blood filter paper, and nested PCR was performed using a specific primer, the pfmdr1-A and pfmdr1-B genes. The PCR cycle was optimized based on previous studies. The pfmdr1-A has a similar setting to the earlier study, but the pfmdr1-B had a different optimum setting from the previous study with the annealing temperature of 57oC for nested-1 and 62oC for nested-2. This PCR setting could be used for further examination. The positive results of the amplification indicated the potential for antimalarial resistance in the parasite population. A study on the gene copy number and polymorphism is essential to determine the definitive conclusion on antimalarial resistance.
Co-Authors Aditha Satria Maulana Alami, Eqiel Navadz Akthar Ancah Caesarina Novi Marchianti Andriani, Made Prasanti Angga Mardro Raharjo, Angga Mardro Arsyzilma Hakiim Ayu Munawaroh Ayu Munawaroh Aziz, Ayu Munawaroh Bachtiar Hidayat, Aldy Bintang, Muhammad Farrel Ravidinata Masoga Caesarina, Ancah Cholis Abrori Dina Helianti Dwita Aryadina Rachmawati Erfan Efendi Erma Sulistyaningsih Erma Sulistyaningsih Erma Sulistyaningsih Gianevan, Nicholas Jirezra Hibatullah, Muhammad Naufal Ida Sri Surani Wiji Astuti Ida Srisurani Wiji Astuti Inke Kusumastuti Irawan Fajar Kusuma Irawan Fajar Kusuma Izza Amalia Putri Jauhar Firdaus Kholifaturrohmah, Sakinatus Sariroh Kholifaturrohmy, Muhammad Rizqi Lusi Padma Sulistianingsih Mata Luthfiyyah, Nur Atikah M. Ali Shodikin Muhammad Afiful Jauhani Muhammad Hasan Mukhammad Arif Hadi Khoiruddin Nizar Fiska Bayu Agustian Nofica, Cheila Nurmaida, Eny Putri, Elvia Rahmi Marga Rahma, Nafisah Hani Asyifah Rena Normasari Rini Riyanti Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rumastika, Nindya Shinta Sahrir Sillehu Sa’diyah, Nindya Audatus Sudarmanto, Yohanes Sulistyaningsih , Erma Sumadi Suryono Suryono Yunita Armiyanti Zahniar, Zahniar