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All Journal Jurnal Ilmu Dasar Jurnal Ilmu Kedokteran (Journal of Medical Science) Majalah Kedokteran Bandung AL KAUNIYAH Journal of Agromedicine and Medical Sciences Elkawnie MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Syntax Literate: Jurnal Ilmiah Indonesia Medika Kartika : Jurnal Kedokteran dan Kesehatan JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jember Medical Journal (JMJ) International Journal of Health and Information System (IJHIS)
Sheilla Rachmania, Sheilla
Department Of Histology, Faculty Of Medicine, University Of Jember, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Liver Histopathological Analysis in The Acute Toxicity Test of Shallot (Allium cepa L.) Peel Extract in Rats (Rattus norvegicus) Aprilya Pratiwi, Nadilla; Dewi, Rosita; Agustina, Dini; Helianti, Dina; Rachmania, Sheilla
Journal of Agromedicine and Medical Sciences Vol. 11 No. 3 (2025)
Publisher : Faculty of Medicine, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v11i3.53736

Abstract

Shallot (Allium cepa L.) contains bioactive compounds with antioxidant effects. In addition to the tuber, the shallot’s peel is a rich source of flavonoids with demonstrated capacity to mitigate oxidative stress. Prior studies have evaluated the antioxidant efficacy of shallot peel in ameliorating organ damage and have established its maximum effective dose. The subsequent step required for its potential therapeutic application is toxicity test. This study aimed to analyze the liver histopathological changes in the acute toxicity test of shallot peel extract (SPE) in rats based on OECD TG 420. This research was an experimental with a post-test-only control group design. Five female rats were used as the control group administered dimethyl sulfoxide (DMSO), while 5 female rats were used in the treatment group administered SPE at 5,000 mg/kg body weight (BW) (one of them had been used for a preliminary test with the same dosage). On day 15, a necropsy was conducted, followed by histopathological observation of the hematoxylin-eosin (HE)-stained liver histopathological slide. The damage to hepatocytes was evaluated using Manja Roenigk criteria. The average histopathological score per liver cell of the control group was 1.226 ± 0.0065 and the treatment group was 1.235±0.0079. The Mann-Whitney test showed that the liver histopathological score of the treatment group was not different from that of the control group (p>0.05). It can be concluded that SPE 5,000 mg/kg does not show acute toxic effects in rats, with LD₅₀ estimated at >5,000 mg/kg according to OECD standards.
Specific Sequence Motif of Var Gene as Predictor of Malaria Outcome Erma Sulistyaningsih; Rosita Dewi; Sheilla Rachmania; Irawan Fajar Kusuma; Sahrir Sillehu
Elkawnie Vol. 10 No. 1 (2024)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v10i1.14808

Abstract

Abstract: The Var gene family encodes for Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP1), a protein responsible for malaria pathogenesis. One of the variants, the var D gene, is hypothesized as a predictor of clinical malaria outcomes. The study aimed to investigate the association between the expression of the var D gene and clinical malaria outcomes. Blood spots on filter paper from uncomplicated and severe malaria patients were collected for DNA and RNA extraction. The RNA was reverse-transcribed into cDNA. DNA and cDNA were amplified by Polymerase Chain Reaction (PCR) technique using specific var D primer, and PCR products were electrophorized in 1% agarose. DNA amplification resulted in double bands of approximately 230 bp and 250 bp in uncomplicated and severe malaria samples. However, the cDNA amplification generated a single band of 230 bp from four out of five severe malaria samples. The existence of band solely in severe malaria transcript suggested its involvement in the pathogenesis of severe malaria. In conclusion, the expression of var D gene-specific sequence can be a potential predictor of severe malaria outcomes.Abstrak: Famili gen var mengkode Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP1), suatu protein yang berperan penting dalam proses patogenesis malaria. Salah satu variannya, gen var D, diduga merupakan prediktor gambaran klinis malaria. Penelitian ini bertujuan untuk mengetahui hubungan antara ekspresi gen var D  dan gambaran klinis malaria. Sampel berupa tetesan darah pada kertas filter dari pasien malaria tanpa komplikasi dan malaria berat dikumpulkan untuk diekstraksi DNA dan RNAnya. RNA selanjutnya di trankripsi reverse menjadi cDNA. DNA dan cDNA diamplifikasi dengan teknik PCR menggunakan primer spesifik var D dan produk PCR dielektroforesis menggunakan agarosa 1 %. Amplifikasi DNA menghasilkan beragam pita berukuran sekitar 230 bp dan 250 bp pada sampel malaria tanpa komplikasi dan malaria berat, tetapi amplifikasi cDNA hanya menunjukkan satu pita berukuran 230 bp pada 4 dari 5 sampel malaria berat. Keberadaan pita hanya pada sampel malaria berat mengindikasikan peran gen ini dalam patogenesis malaria berat. Disimpulkan bahwa gen var D dapat ditemukan pada sampel malaria tanpa komplikasi dan berat, namun ekpresi gen tersebut dapat menjadi prediktor yang potensial  timbulnya manifestasi klinis malaria berat.
Increased of Spleen White Pulp Diameter Post Dbl2β-Pfemp1 Recombinant Protein Injection in Wistar Rats: Pre-Clinical Study for Malaria Vaccine Development Erma Sulistyaningsih; Nindya Audatus Sa’diyah; Irawan Fajar Kusuma; Rosita Dewi; Sheilla Rachmania
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 18 No 1 (2024): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v18i1.7451

Abstract

Malaria is a major infectious disease worldwide, and vaccination is essential for disease control. The Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP1) is a potential malaria vaccine candidate due to its involvement in pathogenesis. Injection of DBL2β-PfEMP1 recombinant protein in animal models induces IgG and CD4+ production and inhibits binding with host endothelial receptors. This study aimed to analyze the spleen immune response by measuring the white pulp diameter. The experimental study used Wistar rats (Rattus norvegicus) that were divided into four groups, a control group and three treatment groups, which were injected with 100, 150, and 200 μg of DBL2β-PfEMP1 protein. Injection was done thrice with three-week intervals (days 0, 21, and 42). On day 56, rats were euthanized, and spleens were prepared for histology examination. The white pulp diameter increased along with increasing the dose of protein. The ANOVA test showed a significant difference between groups (p=0.001). The posthoc Bonferroni test showed a significant difference between the control and the 150 and 200 μg groups and the 100 μg and 200 μg groups. In conclusion, the DBL2β-PfEMP1 recombinant protein injection increased the spleen white pulp diameter in Wistar rats, and the 200 μg dose resulted in the highest increase.
Co-Authors Aisy, Rihadatul Alami, Eqiel Navadz Akthar Ancah Caesarina Novi Marchianti Angga Mardro Raharjo, Angga Mardro Aprilya Pratiwi, Nadilla Arsyzilma Hakiim Ayu Munawaroh Aziz, Ayu Munawaroh Bachtiar Hidayat, Aldy Caesarina, Ancah Cholis Abrori, Cholis Cicih Komariah Desie Dwi Wisudanti Dina Helianti Dini Agustina Dwisaraswati, Salma Aulia Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Erma Sulistyaningsih Erma Sulistyaningsih Erma Sulistyaningsih Erma Sulistyaningsih Gianevan, Nicholas Jirezra Handoko, Adelia Hasya, Aghnia Hibatullah, Muhammad Naufal Ida Sri Surani Wiji Astuti Ida Srisurani Wiji Astuti Inke Kusumastuti Irawan Fajar Kusuma Irawan Fajar Kusuma Izza Amalia Putri Jauhar Firdaus Lusi Padma Sulistianingsih Mata Luthfiyyah, Nur Atikah M. Ali Shodikin Maulana, Aditha Satria Muhammad Afiful Jauhani Muhammad Hasan Mukhammad Arif Hadi Khoiruddin Nindya Audatus Sa’diyah Nizar Fiska Bayu Agustian Nofica, Cheila Nurmaida, Eny Pratama, Muhamad Rizal Hadi Putri, Elvia Rahmi Marga Putri, Izza Amalia Rahma, Nafisah Hani Asyifah Rena Normasari Rini Riyanti Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rumastika, Nindya Shinta Sahrir Sillehu septa surya wahyudi, septa surya Sudarmanto, Yohanes Sulistyaningsih , Erma Sumadi Supangat Supangat Suryono Suryono Taufiq Gemawan Yunita Armiyanti Zahniar, Zahniar