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All Journal Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Disease Prevention and Public Health Journal Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Agroekoteknologi dan Agribisnis Berita Kedokteran Masyarakat The International Journal of Tropical Veterinary and Biomedical Research

Widagdo Sri Nugroho

Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Author-ID : 333537

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary

Published : 26 Documents Claim Missing Document

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Show Abstract | Download Original | Original Source | Check in Google Scholar

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants. Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover national demand. In the future it will be a potential problem to national dairy herd and human health. The aim of this study is to detect MAP in the growing up milk formula. Fifty samples from five established distributors were taken in Bogor. Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer. Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57. Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia. Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR

Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Widagdo Sri Nugroho; Rahmat Setya Adji; Aeth Wahyuni
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herrold’s egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57

Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Widagdo Sri Nugroho; M. Sudarwanto; D.W Lukman; E. Setiyaningsih; E. Usleber
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 4 (2009): DECEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces

Uji Sensitivitas Bakteri Escherichia Coli Isolat Asal Ayam Yang Bereaksi Widagdo Sri Nugroho; M. Haryadi Wibowo
Jurnal Sain Veteriner Vol 23, No 1 (2005): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan untuk mengetahui resistensi bakteri E.coli isolat ayam yang bereaksi positif pada media congo red terhadap preparat ampisilin, streptomisin, dan enrofloksasin. Delapan biak murni bakteri E.coli yang diisolasi dari kasus kolibasilosis ayam diuji afinitasnya terhadap zat warna merah kongo. Bakteri yang bereaksi positif pada media congo red tersebut, kemudian diuji sensitivitasnya menggunakan media agar Mueller-Hinton, metode disk difusi menurut Kirby-Bouer. Disk antibiotika ampisilin, streptomisin, dan enrofloksasin diposisikan menggunakan dropper pada permukaan kultur bakteri tersebut, kemudian diinkubasi selama 24 jam pada suhu 37Â°C. Diameter zona terang disekililing disk antibiotik yang terbentuk, kemudian diukur dalam satuan milimeter. Hasil pengukuran tersebut dibandingkan dengan referensi dan atau menurut standar interpretasi Kirby-Bouer. Hasil penelitian menunjukkan bahwa, seluruh bakteri E. coli isolat asal ayam, mempunyai penampilan penotif mengilcat zat warna merah kongo , ternyata 100 % resisten terhadap preparat ampisilin, streptomisin dan enrofloksasin.

PATOGENISITAS ISOLAT Escherichia con POSITIF CONGO RED PADA TELUR AYAM BEREMBRIO UMUR 12 HARI = PATOGENICITY OF CONGO RED POSITIVE ISOLATE OF Escherichia coil IN THE 12-DAYS OLD CHICKEN EMBRYOS Widagdo Sri Nugroho; M. Haryadi Wibowo; Widya Asmara
Jurnal Sain Veteriner Vol 20, No 1 (2002): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan untuk mengetahui patogenisitas isolat Escherichia coif positif congo red. Escherichia coil dari kasus kolibasilosis ayam diisolasi menggunakan media TSA dan EMB kemudian kemampuan pengikatan warna congo red diuji dengan agar congo red (TSA+0,003% congo red). Tingkat patogenisitas isolat yang mengikat warns congo red dilihat dari uji kematian embrio. Empat isolat E.coli positif conga red (CR+) dan 1 isolat negatif terhadap congo red (CR -) diinokulasikan pada telur berembrio umur 12 hari. Tingkat kematian embrio selama arum bad pascainokulasi masing-masing isolat E.coli positif congo red (500 colony form unit / CM) pada kantung alantois telur berembrio umur 12 hari berbeda antar kelompok. Angka kematian yang diperoleh dari isolat CR + 1, 2, 3, dan 4 masing-masing adalah 10%, 20%, 60% dan 100%. Perubahan anatomi yang tampak yaitu terjadinya perdarahan kulit pada embrio yang mati dan secara mikroskopik lesi-lesi pada hati, jantung, dan limpa menujukkan adanya septisemi. Isolasi dan uji ulang congo red terhadap inokulat positif conga red memperlihatkan bahwa beberapa isolat kehilangan kemampuan mengikat warna conga red. Isolat-isolat tersebut memiliki angka kematian yang rendah (10-20%). Variasi kemampuan isolat mengikat warm conga red memiliki keterkaitan dengan patogenisitasnya.

Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Abdulwahed Ahmed Hassan; Ewald Usleber
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.413

Kajian Lintas Seksional Infeksi Salmonela pada ayam Ras Petelur Berdasarkan Uji Ulas Kloaka di Kabupaten Sleman Yogyakarta Widagdo Sri Nugroho; Setyawan Budiharta; Doddy Yudhabuntara
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Protektivitas Sapi di Kabupaten Kupang Terhadap Penyakit Ngorok (Septicaemia Epizootica) (PROTECTIVITY AGAINST SEPTICAEMIA EPIZOOTICA OF COWS IN KUPANG DISTRICT) Hilda Susiyanti Debora Berek; Widagdo Sri Nugroho; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.774 KB)

Controlling SepticemiaEpizooticae (SE) through vaccination program has been undertaken in KupangDistrict. However, numbers of fatal cases is still being reported. A cross sectional study have been undertakenin order to determine the prevalence and factors affecting herd immunity at farm level in Kupang District.A total of 434 cow’s sera from 87 farmers were collected and further analyzed using Enzyme-linkedImmunosorbent Assay (ELISA). An antibody titer of e”200 ELISA Unit was used as the indicator ofprotective immunity. The prevalence of herd immunity was 73.7% and vaccination coverage was 90.8%.Based on unweighted logistic regression analysis it was found that factors affecting the animals protectiveimmunity were: animal >2 years of age (r = +1.45601; OR = 4.3); housing system ( r = +1.03958; OR = 2.8);reservoir animals (r = +0.090147; OR = 2.5), the sex of the animal (female) (r = + 0.080138; OR =2.2); oncefrequency of vaccination (r = +0.61015; OR = 1.8); and 6-12 months post vaccination period (r = + 0.58968;OR = 1.8). Based on linier regression analysis, the prevalence of herd immunity was increased during rainyseason, whereas it was decreased when cows werebought from animal market or from other district.

Faktor-Faktor Risiko Mastitis Subklinis pada Kambing Peranakan Etawah di Kabupaten Sleman, Yogyakarta (RISK FACTORS OF SUBCLINICAL MASTITIS ON ETTAWA CROSSBRED GOAT IN SLEMAN REGENCY, YOGYAKARTA) Widodo Suwito; Widagdo Sri Nugroho; Bambang Sumiarto; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.338 KB)

In Sleman, a regency in Yogyakarta special region, Etawah crossbred goats are excessively bred for thedairy produce called the goat’s milk. Subclinical mastitis is one of diseases which reduce the yield of goat’smilk. The aim of this study was to determine the risk factors that contribute to the subclinical mastitis onthe Etawah crossbred goats in Sleman. The 200 samples one of which contains 10 mL of goat’s milk weretaken from the udders of the Etawah crossbred goats from the eight goat farms in Sleman. The 200samples were analyzed for the subclinical mastitis using California Mastitis Test (CMT). The data of riskfactors were gathered through a questionnaire. The risk factors on Etawah crossbred goats in Sleman weredetermine with the use of bivariate analysis chi square (X)2, odds ratio (OR) and relative risk (RR). Thegoat’s milk which subclinical mastitis was isolation and identification of bacteria based on biochemicaltests. The risk factors that cause the subclinicall mastitis on Etawah crossbred goats in Sleman were (1)milk yield (X2=14.23; OR=6.52; RR=4.42), (2) age status of lactation (X2=1.60; OR=59.09; RR=17.94), (3)age of weaning (X2=26.06; OR=2.22; RR=1.91), and (4) Body Condition Score (BCS) (X2=13.89; OR=1.29;RR=1.22). Goat’s milk which subclinicall mastitis were isolated Bacillus sp (70%), Staphylococcus sp(33%), Pseudomonas sp (29%), Streptococcus sp (25%), Corynebacterium sp (12%), and E. coli (4%).

Deteksi Mycobacterium Avium Subspesies Paratuberculosis pada Susu Pasturisasi yang Dijual di Bogor Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Ewald Usleber
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.675 KB)

Mycobacterium avium subspesies paratuberculosis (MAP) is a thermal tolerant bacteria. The presenceof these bacteria in pasteurized dairy milk is associated with infectious bowel disease in human known asCrohn’s disease. The aim of this study was to detect MAP in pasteurized dairy milk sold in Bogor. Fourtytwo samples of plain flavoured milk (180–250 ml) from 7 producers were bought from supermarkets inBogor. The presence of MAP was detected by isolation and conventional polymerase chain reaction (PCR)using IS 900 and F57. Bacterial isolation were done by Herrold’s egg yolk medium with mycobactine J(HEYMj) and without mycobactin J (HEYM) and incubated at 37°C for 20 weeks. The DNA extraction ofall pasteurized dairy milk samples were conducted by DNeasy® Tissue Kit. Amplification conditionsfor PCR were: 1 cycle at 94°C for 10 minutes, 40 cycles at 94°C for 1 minute, 58°C for 1 minute, and72°C for 3 minutes, and 1 cycle at 72°C for 7 minutes. After 20 weeks of incubation, there were no sign ofMAP which grew in all isolation mediums. The PCR IS 900 and F57 did not detect the DNA band of thetarget. In the conclusion, there was no MAP detected in pasteurized dairy milk sold in Bogor.

Co-Authors Abdulwahed Ahmed Hassan Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bawono, Gigih D.W Lukman Defryana Rakebsa Denny Widaya Lukman Dian Bekti Hadi Masithoh Doddi Yudhabuntara Doddy Yudhabuntara Dyah Ayu Widiasih Dyah Ayu Widiasih E. Setiyaningsih E. Usleber Erwan Agus Purwanto Etih Sudarnika Ewald Usleber F. Lintang R. Daru Maya Fitriana, Ida Gallantiswara, Putu Cri Devischa Hari Kusnanto Heris Kustiningsih Heru Susetya Hilda Susiyanti Debora Berek Ismadi, Joko Khrisdiana Putri Khrisdiana Putri, Khrisdiana Kusnanto, Hari M. Haryadi Wibowo M. Sudarwanto Maftuhah Nurbeti, Maftuhah Marla Anggita Mazdani Ulfah Daulay Minarwati Sudarwanto Mirnawati Sudarwanto Nurbeti, Maftuhah Prisha Lini Sivalingham Puspa Wikan Sari Raden Gagak Donny Satria Rahmat Setya Adji Rahmat Setya Adji Rahmi, Siwi Meylina Rochman Naim Roza Azizah Primatika Roza Azizah Primatika, Roza Azizah Salim, Devi Andreani Setiawan, Dwi Cahyo Budi Setyawan Budiharta Silalahi, Gustaf Eifel Surachmi Setyaningsih Teguh Budipitojo Toha, Larry Richard Wellem Widodo Suwito Widodo Suwito Widya Asmara Yatri Drastini Yuda Heru Fibrianto Yudhanto, Setyo

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