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All Journal Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Disease Prevention and Public Health Journal Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Agroekoteknologi dan Agribisnis Berita Kedokteran Masyarakat Journal of Biodiversity and Biotechnology Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Widagdo Sri Nugroho
Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary

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Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy he Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Rochman Naim; Ewald Usleber
Forum Pasca Sarjana Vol. 32 No. 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy herd and human health.  The aim of this study is to detect MAP in the growing up milk formula.  Fifty samples from five established distributors were taken in Bogor.  Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer.  Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57.  Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia.   Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR
Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Widagdo Sri Nugroho; Rahmat Setya Adji; Aeth Wahyuni
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.46 KB) | DOI: 10.14334/jitv.v13i2.605

Abstract

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herrold’s egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57
Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Widagdo Sri Nugroho; M. Sudarwanto; D.W Lukman; E. Setiyaningsih; E. Usleber
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 4 (2009): DECEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.452 KB) | DOI: 10.14334/jitv.v14i4.335

Abstract

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces
Uji Sensitivitas Bakteri Escherichia Coli Isolat Asal Ayam Yang Bereaksi Widagdo Sri Nugroho; M. Haryadi Wibowo
Jurnal Sain Veteriner Vol 23, No 1 (2005): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2825.683 KB) | DOI: 10.22146/jsv.364

Abstract

Penelitian ini bertujuan untuk mengetahui resistensi bakteri E.coli isolat ayam yang bereaksi positif pada media congo red terhadap preparat ampisilin, streptomisin, dan enrofloksasin. Delapan biak murni bakteri E.coli yang diisolasi dari kasus kolibasilosis ayam diuji afinitasnya terhadap zat warna merah kongo. Bakteri yang bereaksi positif pada media congo red tersebut, kemudian diuji sensitivitasnya menggunakan media agar Mueller-Hinton, metode disk difusi menurut Kirby-Bouer. Disk antibiotika ampisilin, streptomisin, dan enrofloksasin diposisikan menggunakan dropper pada permukaan kultur bakteri tersebut, kemudian diinkubasi selama 24 jam pada suhu 37°C. Diameter zona terang disekililing disk antibiotik yang terbentuk, kemudian diukur dalam satuan milimeter. Hasil pengukuran tersebut dibandingkan dengan referensi dan atau menurut standar interpretasi Kirby-Bouer. Hasil penelitian menunjukkan bahwa, seluruh bakteri E. coli isolat asal ayam, mempunyai penampilan penotif mengilcat zat warna merah kongo , ternyata 100 % resisten terhadap preparat ampisilin, streptomisin dan enrofloksasin.
PATOGENISITAS ISOLAT Escherichia con POSITIF CONGO RED PADA TELUR AYAM BEREMBRIO UMUR 12 HARI = PATOGENICITY OF CONGO RED POSITIVE ISOLATE OF Escherichia coil IN THE 12-DAYS OLD CHICKEN EMBRYOS Widagdo Sri Nugroho; M. Haryadi Wibowo; Widya Asmara
Jurnal Sain Veteriner Vol 20, No 1 (2002): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5076.058 KB) | DOI: 10.22146/jsv.393

Abstract

Penelitian ini bertujuan untuk mengetahui patogenisitas isolat Escherichia coif positif congo red. Escherichia coil dari kasus kolibasilosis ayam diisolasi menggunakan media TSA dan EMB kemudian kemampuan pengikatan warna congo red diuji dengan agar congo red (TSA+0,003% congo red). Tingkat patogenisitas isolat yang mengikat warns congo red dilihat dari uji kematian embrio. Empat isolat E.coli positif conga red (CR+) dan 1 isolat negatif terhadap congo red (CR -) diinokulasikan pada telur berembrio umur 12 hari. Tingkat kematian embrio selama arum bad pascainokulasi masing-masing isolat E.coli positif congo red (500 colony form unit / CM) pada kantung alantois telur berembrio umur 12 hari berbeda antar kelompok. Angka kematian yang diperoleh dari isolat CR + 1, 2, 3, dan 4 masing-masing adalah 10%, 20%, 60% dan 100%. Perubahan anatomi yang tampak yaitu terjadinya perdarahan kulit pada embrio yang mati dan secara mikroskopik lesi-lesi pada hati, jantung, dan limpa menujukkan adanya septisemi. Isolasi dan uji ulang congo red terhadap inokulat positif conga red memperlihatkan bahwa beberapa isolat kehilangan kemampuan mengikat warna conga red. Isolat-isolat tersebut memiliki angka kematian yang rendah (10-20%). Variasi kemampuan isolat mengikat warm conga red memiliki keterkaitan dengan patogenisitasnya.
Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Abdulwahed Ahmed Hassan; Ewald Usleber
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.413

Abstract

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Kajian Lintas Seksional Infeksi Salmonela pada ayam Ras Petelur Berdasarkan Uji Ulas Kloaka di Kabupaten Sleman Yogyakarta Widagdo Sri Nugroho; Setyawan Budiharta; Doddy Yudhabuntara
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2022.529 KB) | DOI: 10.22146/jsv.474

Abstract

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PREVALENSI SEROPOSITIF DAN CAKUPAN VAKSINASI ANTRAKS PADA SAPI DI KABUPATEN SRAGEN DAN BOYOLALI Heris Kustiningsih; Widagdo Sri Nugroho
Jurnal Agroekoteknologi dan Agribisnis Vol. 1 No. 1 (2017)
Publisher : Politeknik Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5794.758 KB) | DOI: 10.51852/jaa.v1i1.399

Abstract

Anthrax is a zoonotic disease caused by Bacillus anthracis, and this disease caused economic loss. Prevention and control of anthrax in Central Java, particularly in Sragen and Boyolali has been done, however, the incidence of anthrax still continue to be reported. This study aims determined the prevalence of anthrax seropositive postvaccination, coverage vaccination and evaluation of efectivity anthrax vaccinations in cattle in Sragen and Boyolali dictrict. Population target were cattle in high risk area in sub-districts in Sragen and Boyolali namely Miri, Tanon, Klego, and Andong. Samples of farmers were selected by simple random sampling and the cattles were taken by cluster sampling, respetively. With the assumption of two cattle ownership per farmer, 210 farmers with 358 cattle were selected. Serology testing was performed by ELISA method. Data was analyzed using 7th version of statistix (analytical software Inc.). Analysis of data includes statistic description. Coverage anthrax vaccination in both districts were 68,2% from 358 cows. Comulative seropositive antibody against anthrax pastvaccination reached 4,9% but in Sragen it self reached 13.2% and in Boyolali only 1.2%. Prevalence of anthrax seropositive postvaccination and coverage vaccination in cattle in Sragen and Boyolali were relatively low. Anthrax vaccinations in both the district did not effective, so that vaccination program should to be improved. Anthrax vaccinations in sragen district more effective than in boyolali Antraks adalah penyakit zoonotik yang disebabkan oleh Bacillus anthracis.Kerugian ekonomi yang diakibatkan penyakit ini sangat besar. Upaya pencegahan dan pengendalian antraks di Jawa Tengah khususnya di Kabupaten Sragen dan Boyolali telah dilakukan, namun demikian kejadian antraks masih terus dilaporkan. Penelitian ini bertujuan untuk mengetahui prevalensi seropositif pascavaksinasi, cakupan vaksinasinya serta mengevaluasi efektivitas vaksinasi antraks pada ternak sapi di Kabupaten Sragen dan Kabupaten Boyolali. Populasi target adalah sapi-sapi yang berada di kecamatan-kecamatan tertular antraks, baik di Kabupaten Sragen maupun Kabupaten Boyolali yaitu Kecamatan Miri, Tanon, Klego dan Andong. Sampel peternak dipilih secara random sederhana dan pemilihan sampel ternak secara kluster, dengan asumsi kepemilikan per peternak dua ekor, dipilih 210 peternak dengan total ternak sebanyak 358 ekor.Pengujian serum dilakukan dengan ELISA. Data disimpan dan diolah menggunakan statistix versi 7th(analytical software /nc.).Hasil penelitian ini, cakupan vaksinasi antraks di Kabupaten Sragen dan Boyolali mencapai 68,2% dari 358 ekor sapi. Prevalensi seropositif terhadap antraks di Kabupaten Sragen dan Boyolali adalah 4,8%. Total seropositif terhadap antraks pada sapi yang divaksin mencapai 4,9%, di Kabupaten Sragen sendiri mencapai 13,2% dan Kabupaten Boyolali hanya 1,2%.Prevalensi seropositif dan cakupan vaksinasi antraks di Kabupaten Sragen dan Boyolali masih cukup rendah. Vaksinasi antraks di kedua kabupaten tersebut belum efektif, sehingga peningkatkan efektivitas vaksinasi perlu terus ditingkatkan.Vaksinasi antraks di Kabupaten Sragen lebih efektif bila dibandingkan di Kabupaten Boyolali. 
Pengetahuan, sikap, dan faktor risiko lingkungan pada rumah tangga dengan riwayat leptospirosis di kota Yogyakarta dan kabupaten Bantul Defryana Rakebsa; Citra Indriani; Widagdo Sri Nugroho
Berita Kedokteran Masyarakat (BKM) Vol 34, No 4 (2018)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.309 KB) | DOI: 10.22146/bkm.28562

Abstract

Epidemiology of leptospirosis in Yogyakarta and BantulPurposeThe purpose of this study was to determine the relationship knowledge, attitude, and environmental factors with the incidence of leptospirosis.MethodsA case-control study was conducted in November 2016 in Yogyakarta city and Bantul district on 242 respondents. The case was leptospirosis patients that recorded in the Yogyakarta and Bantul District health offices within the period of June 2014 to June 2016. Controls were households in the same neighborhood as the case and had no history of leptospirosis, with case and control comparisons 1:1.  Rat-catching was done inside the house and in the rice fields. ResultsKnowledge (OR: 1, 95% CI: 0.58-1.71, p-value: 1) and attitude (OR: 0.8, 95% CI: 0.47-1.40, p-value: 0 .44) had no significant association with leptospirosis incidence. The presence of rats in the home has no significant relationship with leptospirosis incidence (OR: 0.7, 95% CI: 0.15-3.78, p-value: 0.73). The multivariate analysis found that the distance of house to open sewer (OR: 2.96, 95% CI: 1.22-7.14) and the presence of in-house waste (OR: 2.03, 95% CI: 1.14-3.62) had a significant association with leptospirosis incidence.ConclusionThere was no statistically significant relationship between knowledge and attitude with leptospirosis incidence. Environmental factors such as the distance of house to open sewer and the presence of in-house waste have a statistically significant relationship with the incidence of leptospirosis. Two of the 4 serum mice examined with a positive MAT method contained Leptospira sp. with Benjamin serovar Benjamini strain.
PATH ANALYSIS TINGKAT PENGETAHUAN, SIKAP DAN PRAKTIK PETERNAK BABI TERHADAP PENGENDALIAN HOG CHOLERA DI KECAMATAN KOTA RAJA KOTA KUPANG Toha, Larry Richard Wellem; Susetya, Heru; Nugroho, Widagdo Sri
JURNAL KAJIAN VETERINER Vol 8 No 2 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i2.3003

Abstract

Pig population in East Nusa Tenggara Province in 2019 was recorded at around 2 million pigs. In east Nusa Tenggara around 85% of the households raise at least 1 (one) pig per household. Pig farming industry has its own challenges and resistances, one of the challenge is the threat of infectious diseases such as Hog cholera disease. Hog cholera is a disease caused by virus and has become endemic in most of the area in East Nusa Tenggara with relatively high prevalence. In Kupang City the prevalence of HC in 2018 was recorded at around 20,5%. The aim of this study is to analyse the relationship of pig farmer characteristics with knowledge level, attitude and practice regarding HC controlling and eradication in Kota Raja Sub-distric Kupang City. In this study, data was obtained by questionnaire and interview of pig farmers which was done in Kota Raja Sub-distric from August until October 2018. Data was analyzed with descriptive statistic and KAP studi data was analyzed with path analysis to measure the relationship between observed characteristics and HC controlling practice. The result of this study shows that variable that has the stongest relationship to practice is level of education with path coefficient (r) of -0,438 (P 0,027), followed by attitude with path coefficient (r) of 0,233 (P 0,000), and then followed by knowledge with path coefficient (r) of 0,224 (P 0,008) and the weakest realationship to practice is farmers age with path coefficient (r) of -0,049 (P 0,016).
Co-Authors Abdulwahed Ahmed Hassan AETH Wahyuni Wahyuni, AETH Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agustia, Agustia Agustina Dwi Wijayanti Aisah, Rahma Hani Amaliah, Fitri Aprizal Panus, Aprizal Aris Haryanto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bawono, Gigih D.W Lukman Defryana Rakebsa Denny Widaya Lukman Dian Bekti Hadi Masithoh Doddi Yudhabuntara Doddy Yudhabuntara Dyah Ayu Widiasih Dyah Ayu Widiasih E. Setiyaningsih E. Usleber Erwan Agus Purwanto Etih Sudarnika Ewald Usleber F. Lintang R. Daru Maya Fitriana, Ida Gallantiswara, Putu Cri Devischa Hari Kusnanto Hartawan, Dinar Hadi Wahyu Heris Kustiningsih Heru Susetya Hilda Susiyanti Debora Berek Ismadi, Joko Kajang, Elphan Augusta Khrisdiana Putri Khrisdiana Putri, Khrisdiana Kusnanto, Hari M. Haryadi Wibowo M. Sudarwanto Maftuhah Nurbeti, Maftuhah Marla Anggita Mazdani Ulfah Daulay Minarwati Sudarwanto Mirnawati Sudarwanto Nurbeti, Maftuhah Primasito, Anggit Prisha Lini Sivalingham Puspa Wikan Sari Raden Gagak Donny Satria Rahmat Setya Adji Rahmat Setya Adji Rahmi, Siwi Meylina Rochman Naim Roza Azizah Primatika Roza Azizah Primatika, Roza Azizah Safitri, Ivana Dewi Salim, Devi Andreani Setiawan, Dwi Cahyo Budi Setyawan Budiharta Silalahi, Gustaf Eifel Sodirun, Sodirun Surachmi Setyaningsih Teguh Budipitojo Toha, Larry Richard Wellem Tri Untari Wibawa, Hendra Widodo Suwito Widodo Suwito Widodo Suwito Widya Asmara Yatri Drastini Yuda Heru Fibrianto Yudhanto, Setyo