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All Journal Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Disease Prevention and Public Health Journal Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Agroekoteknologi dan Agribisnis Berita Kedokteran Masyarakat The International Journal of Tropical Veterinary and Biomedical Research
Widagdo Sri Nugroho
Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary

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Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) Mazdani Ulfah Daulay; Mirnawati Sudarwanto; Widagdo Sri Nugroho; Etih Sudarnika
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.001 KB)

Abstract

Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Löwenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research.
PREVALENSI SEROPOSITIF DAN CAKUPAN VAKSINASI ANTRAKS PADA SAPI DI KABUPATEN SRAGEN DAN BOYOLALI Heris Kustiningsih; Widagdo Sri Nugroho
Jurnal Agroekoteknologi dan Agribisnis Vol. 1 No. 1 (2017)
Publisher : Politeknik Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5794.758 KB) | DOI: 10.51852/jaa.v1i1.399

Abstract

Anthrax is a zoonotic disease caused by Bacillus anthracis, and this disease caused economic loss. Prevention and control of anthrax in Central Java, particularly in Sragen and Boyolali has been done, however, the incidence of anthrax still continue to be reported. This study aims determined the prevalence of anthrax seropositive postvaccination, coverage vaccination and evaluation of efectivity anthrax vaccinations in cattle in Sragen and Boyolali dictrict. Population target were cattle in high risk area in sub-districts in Sragen and Boyolali namely Miri, Tanon, Klego, and Andong. Samples of farmers were selected by simple random sampling and the cattles were taken by cluster sampling, respetively. With the assumption of two cattle ownership per farmer, 210 farmers with 358 cattle were selected. Serology testing was performed by ELISA method. Data was analyzed using 7th version of statistix (analytical software Inc.). Analysis of data includes statistic description. Coverage anthrax vaccination in both districts were 68,2% from 358 cows. Comulative seropositive antibody against anthrax pastvaccination reached 4,9% but in Sragen it self reached 13.2% and in Boyolali only 1.2%. Prevalence of anthrax seropositive postvaccination and coverage vaccination in cattle in Sragen and Boyolali were relatively low. Anthrax vaccinations in both the district did not effective, so that vaccination program should to be improved. Anthrax vaccinations in sragen district more effective than in boyolali Antraks adalah penyakit zoonotik yang disebabkan oleh Bacillus anthracis.Kerugian ekonomi yang diakibatkan penyakit ini sangat besar. Upaya pencegahan dan pengendalian antraks di Jawa Tengah khususnya di Kabupaten Sragen dan Boyolali telah dilakukan, namun demikian kejadian antraks masih terus dilaporkan. Penelitian ini bertujuan untuk mengetahui prevalensi seropositif pascavaksinasi, cakupan vaksinasinya serta mengevaluasi efektivitas vaksinasi antraks pada ternak sapi di Kabupaten Sragen dan Kabupaten Boyolali. Populasi target adalah sapi-sapi yang berada di kecamatan-kecamatan tertular antraks, baik di Kabupaten Sragen maupun Kabupaten Boyolali yaitu Kecamatan Miri, Tanon, Klego dan Andong. Sampel peternak dipilih secara random sederhana dan pemilihan sampel ternak secara kluster, dengan asumsi kepemilikan per peternak dua ekor, dipilih 210 peternak dengan total ternak sebanyak 358 ekor.Pengujian serum dilakukan dengan ELISA. Data disimpan dan diolah menggunakan statistix versi 7th(analytical software /nc.).Hasil penelitian ini, cakupan vaksinasi antraks di Kabupaten Sragen dan Boyolali mencapai 68,2% dari 358 ekor sapi. Prevalensi seropositif terhadap antraks di Kabupaten Sragen dan Boyolali adalah 4,8%. Total seropositif terhadap antraks pada sapi yang divaksin mencapai 4,9%, di Kabupaten Sragen sendiri mencapai 13,2% dan Kabupaten Boyolali hanya 1,2%.Prevalensi seropositif dan cakupan vaksinasi antraks di Kabupaten Sragen dan Boyolali masih cukup rendah. Vaksinasi antraks di kedua kabupaten tersebut belum efektif, sehingga peningkatkan efektivitas vaksinasi perlu terus ditingkatkan.Vaksinasi antraks di Kabupaten Sragen lebih efektif bila dibandingkan di Kabupaten Boyolali. 
Pengetahuan, sikap, dan faktor risiko lingkungan pada rumah tangga dengan riwayat leptospirosis di kota Yogyakarta dan kabupaten Bantul Defryana Rakebsa; Citra Indriani; Widagdo Sri Nugroho
Berita Kedokteran Masyarakat (BKM) Vol 34, No 4 (2018)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.309 KB) | DOI: 10.22146/bkm.28562

Abstract

Epidemiology of leptospirosis in Yogyakarta and BantulPurposeThe purpose of this study was to determine the relationship knowledge, attitude, and environmental factors with the incidence of leptospirosis.MethodsA case-control study was conducted in November 2016 in Yogyakarta city and Bantul district on 242 respondents. The case was leptospirosis patients that recorded in the Yogyakarta and Bantul District health offices within the period of June 2014 to June 2016. Controls were households in the same neighborhood as the case and had no history of leptospirosis, with case and control comparisons 1:1.  Rat-catching was done inside the house and in the rice fields. ResultsKnowledge (OR: 1, 95% CI: 0.58-1.71, p-value: 1) and attitude (OR: 0.8, 95% CI: 0.47-1.40, p-value: 0 .44) had no significant association with leptospirosis incidence. The presence of rats in the home has no significant relationship with leptospirosis incidence (OR: 0.7, 95% CI: 0.15-3.78, p-value: 0.73). The multivariate analysis found that the distance of house to open sewer (OR: 2.96, 95% CI: 1.22-7.14) and the presence of in-house waste (OR: 2.03, 95% CI: 1.14-3.62) had a significant association with leptospirosis incidence.ConclusionThere was no statistically significant relationship between knowledge and attitude with leptospirosis incidence. Environmental factors such as the distance of house to open sewer and the presence of in-house waste have a statistically significant relationship with the incidence of leptospirosis. Two of the 4 serum mice examined with a positive MAT method contained Leptospira sp. with Benjamin serovar Benjamini strain.
PATH ANALYSIS TINGKAT PENGETAHUAN, SIKAP DAN PRAKTIK PETERNAK BABI TERHADAP PENGENDALIAN HOG CHOLERA DI KECAMATAN KOTA RAJA KOTA KUPANG Toha, Larry Richard Wellem; Susetya, Heru; Nugroho, Widagdo Sri
JURNAL KAJIAN VETERINER Vol 8 No 2 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i2.3003

Abstract

Pig population in East Nusa Tenggara Province in 2019 was recorded at around 2 million pigs. In east Nusa Tenggara around 85% of the households raise at least 1 (one) pig per household. Pig farming industry has its own challenges and resistances, one of the challenge is the threat of infectious diseases such as Hog cholera disease. Hog cholera is a disease caused by virus and has become endemic in most of the area in East Nusa Tenggara with relatively high prevalence. In Kupang City the prevalence of HC in 2018 was recorded at around 20,5%. The aim of this study is to analyse the relationship of pig farmer characteristics with knowledge level, attitude and practice regarding HC controlling and eradication in Kota Raja Sub-distric Kupang City. In this study, data was obtained by questionnaire and interview of pig farmers which was done in Kota Raja Sub-distric from August until October 2018. Data was analyzed with descriptive statistic and KAP studi data was analyzed with path analysis to measure the relationship between observed characteristics and HC controlling practice. The result of this study shows that variable that has the stongest relationship to practice is level of education with path coefficient (r) of -0,438 (P 0,027), followed by attitude with path coefficient (r) of 0,233 (P 0,000), and then followed by knowledge with path coefficient (r) of 0,224 (P 0,008) and the weakest realationship to practice is farmers age with path coefficient (r) of -0,049 (P 0,016).
Prevalence and Risk Factors of Lumpy Skin Disease in Pakem District, Yogyakarta Rahmi, Siwi Meylina; Salim, Devi Andreani; Primatika, Roza Azizah; Nugroho, Widagdo Sri; Susetya, Heru; Widiasih, Dyah Ayu; Bawono, Gigih
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.30-36

Abstract

Lumpy Skin Disease (LSD) is an infectious dermatological condition affecting ruminant animals, particularly cattle, and is caused by the Lumpy Skin Disease Virus (LSDV). This virus possesses DNA genetic material derived from the Capripoxvirus genus within the Poxviridae family. The World Organization for Animal Health (WOAH) states that LSD has a mortality of 1-5% and a morbidity of 10-20% and can spread quickly if not handled properly. This study aimed to determine the prevalence of LSD and to identify associated risk factors in Pakem District, Yogyakarta. This research used a cross-sectional study. Samples were taken using a double-stage sampling technique with a total sample of 262 cattle. The study employed primary data collected through interviews with farmers. Data was analyzed descriptively and analytically. Data in the form of frequencies and percentages were analyzed using univariate methods. The relationship between risk factors and the incidence of LSD was analyzed using bivariate Chi-Square methods. The results showed that the prevalence of LSD in the Pakem District was 7.6%. The presence of ticks was identified as a risk factor for LSD disease, with a probability value of p<0.05. The association’s strength was indicated by an odds ratio (OR) of 2.64, suggesting that ticks may elevate the risk of contracting LSD by a factor of 2.64. The prevalence of LSD in Pakem District was 7.6%, and the presence of ticks 2.64 times increases the risk of LSD incidents. Control measures can be improved by carrying out livestock management in handling vectors.
Antibiotic resistance in Escherichia coli from livestock-related sources: A cross-sectional study of feces, soil, and water in a non-urban region Silalahi, Gustaf Eifel; Widiasih, Dyah Ayu; Susetya, Heru; Nugroho, Widagdo Sri; Drastini, Yatri; Putri, Khrisdiana; Gallantiswara, Putu Cri Devischa; Yudhanto, Setyo; Primatika, Roza Azizah
The International Journal of Tropical Veterinary and Biomedical Research Vol 10, No 1 (2025): Vol 10 (1) May 2025
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v10i1.46238

Abstract

Background and Aim: Antibiotics are one of the most used treatments by farmers, both in commercial and non-commercial livestock operations. The use of antibiotics in animals directly impacts the environment, which can become a significant factor in the spread of antibiotic resistance. The environment plays an essential role for both animals and humans, and contamination caused by uncontrolled environmental factors can lead to health impacts on surrounding populations. One of the contaminants that can threaten the health of animals and humans is the presence of Escherichia coli. The aim of this study was to determine the antibiotic resistance profile of E. coli in environmental samples collected from a non-urban livestock region in Playen, Gunung Kidul Regency, Yogyakarta.Materials and Methods: The data in this study consisted of fifty samples, including fishpond water, wastewater, drinking water, water source, water tank, feces, and soil. The methodology involved bacterial isolation using biochemical tests and antimicrobial resistance testing conducted through the KirbyBauer disk diffusion method.Results: The antibiotics used in this study were streptomycin, penicillin, enrofloxacin, oxytetracycline, and sulfamethoxazole. The results showed that 5% of the samples were resistant to streptomycin, 92% were resistant to penicillin, 0% were resistant to enrofloxacin, 12.8% were resistant to oxytetracycline, and 2.5% were resistant to sulfamethoxazole.Conclusion: The findings indicate a high prevalence of Penicillin-resistant E. coli in environmental samples from non-urban livestock areas, highlighting the potential risk of environmental antibiotic resistance transmission.
Co-Authors Abdulwahed Ahmed Hassan Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bawono, Gigih D.W Lukman Defryana Rakebsa Denny Widaya Lukman Dian Bekti Hadi Masithoh Doddi Yudhabuntara Doddy Yudhabuntara Dyah Ayu Widiasih Dyah Ayu Widiasih E. Setiyaningsih E. Usleber Erwan Agus Purwanto Etih Sudarnika Ewald Usleber F. Lintang R. Daru Maya Fitriana, Ida Gallantiswara, Putu Cri Devischa Hari Kusnanto Heris Kustiningsih Heru Susetya Hilda Susiyanti Debora Berek Ismadi, Joko Khrisdiana Putri Khrisdiana Putri, Khrisdiana Kusnanto, Hari M. Haryadi Wibowo M. Sudarwanto Maftuhah Nurbeti, Maftuhah Marla Anggita Mazdani Ulfah Daulay Minarwati Sudarwanto Mirnawati Sudarwanto Nurbeti, Maftuhah Prisha Lini Sivalingham Puspa Wikan Sari Raden Gagak Donny Satria Rahmat Setya Adji Rahmat Setya Adji Rahmi, Siwi Meylina Rochman Naim Roza Azizah Primatika Roza Azizah Primatika, Roza Azizah Salim, Devi Andreani Setiawan, Dwi Cahyo Budi Setyawan Budiharta Silalahi, Gustaf Eifel Surachmi Setyaningsih Teguh Budipitojo Toha, Larry Richard Wellem Widodo Suwito Widodo Suwito Widya Asmara Yatri Drastini Yuda Heru Fibrianto Yudhanto, Setyo