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All Journal VALENSI Atom Indonesia Journal Jurnal Sains Materi Indonesia Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) INDONESIAN JOURNAL OF PHARMACY Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal SOLMA Indonesian Journal of Chemistry JMM (Jurnal Masyarakat Mandiri) JSFK (Jurnal Sains Farmasi & Klinis) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Jamu Indonesia Journal of Halal Science and Research Jurnal Natural Jurnal Pengabdian Masyarakat Bangsa Jurnal Sains dan Kesehatan Jurnal Abdita Naturafarm Indonesian Journal of Jamu
Hariyanti Hariyanti
Fakultas Farmasi Dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Journal : Journal of Halal Science and Research

 1 
The study of pig dna analysis methods in cosmetics: a review Ratu Amada Sofiyani; Hariyanti Hariyanti; Hanifah Rahmi
Journal of Halal Science and Research Vol. 2 No. 1 (2021): February
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (761.76 KB) | DOI: 10.12928/jhsr.v2i1.5177

Abstract

Collagen is one of the components used for cosmetics. Functionally, collagen is derived from pig fat, which functions as a skin conditioning agent (Emollient), emulsion stabilizer (Emulsifier), and as ingredient to increase the viscosity of cosmetic preparations. The method that can detect pig content is the PCR method, which can confirm the presence of DNA content in pigs. In this study, an analysis of cosmetic products containing porcine DNA was carried out using the PCR method. This research was divided into two stages: the initial stage was DNA extraction, and the second stage was PCR analysis. This research is a study through literature study using the narrative review technique. This study aimed to collect information and examine the analysis method of identifying pig DNA contained in cosmetics to obtain the right and accurate way through a literature approach. From the literature study results, it can be concluded that the best extraction method for the isolation of porcine DNA in cosmetic preparations is the extraction method with the Boom Method, while the DNA analysis used to identify porcine DNA in cosmetics is RT-PCR (Real-time Polymerase Chain Reaction). Conventional PCR is because both methods have their respective advantages. The RT-PCR (Real-time Polymerase Chain Reaction) method can amplify and quantify the number of target DNA molecules. The Conventional PCR Method can form DNA bands based on the amplification value. 
Narrative review: a study of pork DNA analysis methods in food gelatin Nisa Aulia Ansor; Hariyanti Hariyanti; Hanifah Rahmi
Journal of Halal Science and Research Vol. 4 No. 2 (2023): September
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v4i2.7798

Abstract

Gelatin is a substance resulting from the partial hydrolysis of collagen consisting of animal cartilage, skin, and bones from water-soluble polypeptides. The problem formulation in this study is what DNA analysis methods can be used to detect pig DNA in food gelatin. This study aimed to collect, review, and conclude information regarding the most widely used DNA analysis method to detect pig DNA in food gelatin. From the results of the literature study, it can be concluded that the most widely used extraction method in the analysis of porcine DNA in food gelatin is the DNeasy® Mericon Food kit extraction method for good purity results, there is the foodproof® III Kit method, the universal Wizard KIT Promega®, the DNeasy® Mericon Food kit, and the Column based FavorPrep™ Food DNA Extraction Kit. The pig DNA analysis method is widely used in conventional PCR. Sensitivity, fast testing time, and gh acporcine gelatin sample was identified by the appearance of a band on the electrophoresis results. Keywords: Food Gelatin, Extraction Method, DNA Analysis, Journal Review.
Determination of gelatin from marshmallows using a combination of fourier transform infrared (ATR-FTIR) spectroscopic and chemometrics for halal authentication Sofia Fatmawati; Hariyanti Hariyanti; Etin Diah Permanasari; Ady Chandra; Johan Dwi Firmanto; Retno Lia Sari; Septiana Tri Pamungkas
Journal of Halal Science and Research Vol. 5 No. 2 (2024): September
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v5i2.10537

Abstract

Marshmallow is a foam that contains aerated sugar, which is stabilized with gelatin or egg albumin. In this research, a Fourier Transform Infra-Red (ATR-FTIR) spectroscopy combined with the Chemometrics methods is developed to distinguish the presence of porcine gelatin in marshmallows, which can then be used to identify halal products. This method provides fast and rapid testing of halal products. Marshmallows were made with varying concentrations of bovine and porcine gelatine as the reference. Commercial marshmallows were collected in the online marketplace by purposive sampling. Isolated gelatin was analyzed using the ATR-FTIR spectrophotometer, and data analysis was continued using PCA (Principal Component Analysis). The results showed that bovine gelatin absorbed at a wavenumber of 1638 cm-1, while porcine gelatin produced a sharp absorption at 1697 and 1654 cm-1. The results of PCA analysis show that 100% of bovine gelatin marshmallows (S100) have areas different from marshmallows containing a mixture of porcine gelatin. The PCA results of four samples (A1, A2, A3, and A4) show they are in the same area as 100% bovine gelatin marshmallows (S100). This shows that it is suspected that the four marshmallow samples tested did not contain porcine gelatin. The multivariate regression curve showed that the pattern of linear absorbance changes along with porcine gelatin concentrations with the highest coefficient equation is from wavenumber 1093.97 cm-1.   Keywords: ATR-FTIR, Bovine gelatin, Chemometric, Marshmallow, Porcine gelatin
Co-Authors Adia Putra Wirman Aditya Sultan Ady Chandra Andzar Fikranus Shofa Anisa Amalia Ari Widayanti Arry Yanuar Aulia, Cahya Aziz Setiawan Azminah Azminah Bariroh, Tahyatul Cahya Aulia Candra, Ricky Crista D. P. Perkasa Devi Wulandari Dewi Susilawati Dewi, Elvira Ratna Dina Pratiwi Ditri Risska Rahmadhani Dwitiyanti Dwitiyanti E Erizal E Erizal E. Mustikarani Erizal Erizal Erizal Erizal Etin Diah Permanasari F. Lukitowati Fadlina Chany Saputri Fajar lukitowati Fatmawati, Sofia Fatmawati, Sofia Firmanto, Johan Dwi Fith Khaira Nursal Fujianti, Fujianti Gantini, Sri Nevi H. Rahmi Hadi Sunaryo Hadi Sunaryo HANIFAH RAHMI Hanifah Rahmi Hanifah Rahmi Hayun Hayun Hayun Hayun, Hayun Hayun, Hayun I. Lestari Indah Lestari Isra, Rafiq Al Johan Dwi Firmanto Khusnun Nafi’ah Kori Yati Kusmadi Kurmardi Lestari, Pramulani Mulya M Yasin Yunus Merina Merina Muhammad Hanafi Ni Putu Ermi Hikmawanti Nining Nisa Aulia Ansor Numlil Khaira Rusdi, Numlil Khaira Nur Azizah Nur Rahmawati Nu’aiman Nu’aiman Oktafiani, Erna Priyanto Priyanto Priyo Wahyudi R. Z. Apriyani Rahmat, Amalia Rahmayanti, Mice Ratu Amada Sofiyani Retno Lia Sari Rina Putri Ariyanti Rini Prastiwi Ririh Zikriah Rizky Arcinthya Rachmania Salahuddin Salahuddin Sari Nurlaily Septiana Tri Pamungkas Sri Nevi Gantini Sri Nevi Gantini Sudirman Sudirman Sulistioso G S Supandi Tafriani Sucianti Nesha Vesya Putri Viransa Viransa, Vesya Putri Yuniarto, Ari Z Juniarti Zulpahmi Zulpahmi