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Identifikasi Keragaman Gen FSH Bagian Ekson 2 Menggunakan Enzim Restriksi TasI pada Sapi Pesisir Tinda Afriani; Endang Purwati; James Hellyward; Jaswandi Jaswandi; Yurnalis Yurnalis; Mangku Mundana; Anna Farhana; Adisti Rastosari
Jurnal Peternakan Vol 19, No 2 (2022): September 2022
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v19i2.17228

ABSTRAK. Studi ini bertujuan untuk mengidentifikasi keragaman gen FSH bagian ekson 2 menggunakan enzim restriksi TasI pada sapi Pesisir. Sampel darah yang digunakan yaitu 78 sampel darah dengan dua macam yaitu 13 jantan serta 65 betina yang berumur 1-5 tahun dari sapi pesisir yang didapat dari BPTUHPT Padang Mengatas. Protocol genomic DNA Purification system Kit dari Promega digunakan untuk mengisolasi DNA sampel darah. Amplifikasi gen FSH dilakukan dengan menggunakan teknik PCR atau Polymerase Chain Reaction. Enzim TasI digunakan untuk meretriksi produk amplifikasi. Hasil penelitian gen FSH bagian exon 2 pada sapi pesisir yaitu diperoleh terdapat dua macam genotip. Dua macam genotip tersebut yaitu heterozigot (+/-) serta homozigot tidak terpotong (-/-), untuk jantan berturut turut yaitu 0,38 dan 0,62 beserta frekuensi alel (+) dan alel (-) yaitu 0,19 dan 0,81, sedangkan untuk betina yaitu berturut-turut 0,43 dan 0,57 beserta frekuensi alel (+) dan alel (-) yaitu 0,21 dan 0,79. Sebaran genotip gen FSH|TasI exon 2 pada populasi Sapi Pesisir sifatnya polimorfik (beragam) dengan frekuensi alel yaitu kurang dari 0,99.Identification of Exon 2 FSH Gene Diversity Using TasI Restriction Enzymes in Coastal CattleABSTRACT. This study aims to identify the diversity of the exon 2 FSH gene using the restriction enzyme TasI in Coastal cattle. The blood samples used were 78 blood samples of two types, namely 13 males and 65 females aged 1-5 years, from coastal cattle obtained from BPTUHPT Padang Atas. The protocol genomic DNA Purification System Kit from Promega was used to isolate DNA from blood samples. The amplification of the FSH gene was carried out using the PCR or Polymerase Chain Reaction technique. The TasI enzyme was used to restrict the amplification product. The study results on the exon two portion of the FSH gene in coastal cattle showed two types of genotypes. The two types of genotypes are heterozygous (+/-) and untruncated homozygous (-/-); for males, 0.38 and 0.62, respectively, and the frequencies of allele (+) and allele (-) are 0.19 and 0. .81, while for females, it was 0.43 and 0.57, respectively, and the frequencies (+) and allele (-) were 0.21 and 0.79. The FSH|TasI exon two gene genotype distribution in the Coastal Cattle population is polymorphic (various) with an allele frequency of less than 0.99.Keywords: FSH Gene, Pesisir Cattle, TasI Enzyme

Identification of Single Nucleotide Polymorphism (SNP) in Early Exon 10 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle Tinda Afriani; Endang Purwati; James Hellyward; Jaswandi Jaswandi; Mangku Mundana; Anna Farhana; Adisti Rastosari
JURNAL ILMIAH PETERNAKAN TERPADU Vol 10, No 3 (2022)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v10i3.p264-276

The purpose of this study was to identify genetic diversity in the early exon 10 of the FSHR gene or follicle stimulating hormone receptor in Pesisir cattle. The blood samples used were 70 blood samples from female Pesisir cattle aged 2-5 years which were obtained from BPTU-HPT Padang Mengatas, West Sumatera. The isolated DNA was then amplified using a pair of primers L and R with PCR technique which produced a fragment with a length of 847 bp. The services of 1st Base Singapore are used for sequencing the amplified product. The results of the research on the identification of the early exon 10 FSHR gene in Pesisir cattle were found that there were 5 SNPs at positions --53T>C, +17A>G, +650C>T, +706A>C and +707 ins>A in some introns 9 to exon 10 first part. The results showed that the genotypic frequency of the Pesisir cattle population was in Hardy-Weinberg imbalance and polymorphisms were found in the early exon 10 FSHR gene diversity.

ANALISIS HUBUNGAN FAKTOR PERSONAL KONSUMEN TERHADAP KEPUTUSAN PEMBELIAN AYAM KAMPUNG DI KOTA PADANG Tevina Edwin; Ida Indrayani; Yolani Utami; Adisti Rastosari
Wahana Peternakan Vol. 7 No. 1 (2023): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v7i1.779

This study aims to look at the relationship between personal factors and purchasing decisions for Kampung chicken meat. Because the market is a place for the implementation of the demand and supply processes, it is able to provide products that are in accordance with buyer requests. This research was conducted in Padang city, West Sumatra, to be precise, at the Padang City Market, which is the central market of this area. The research was carried out in September 2022. The sampling technique used was the convenience sampling technique, and the number of samples was 100 people. The research instrument employed closed questionnaires with research variables such as age (X1), education (X2), family size (X3), income (X4), and purchasing decisions (Y). The Spearman rank test was used to analyze data using non-parametric statistical tests. Through the results of the study, it was found that only education had a close relationship with purchasing decisions. Other factors such as age, the number of family members, and income do not have a close relationship with purchasing decisions. The absence of a significant relationship can be influenced by factors outside the factors studied. Keyword : Personality factor, Meat, Kampung Chicken, Padang

Kombinasi Media Tanam Berbeda Terhadap Pertumbuhan Indigofera zollingeriana Yolani Utami; Suyitman Suyitman; Adisti Rastosari; Tevina Edwin; El Latifa Sri Suharto
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 25, No 2 (2023): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.25.2.240-245.2023

Indigofera zollingeriana salah satu tanaman pakan leguminosa yang memiliki kandungan nutrisi dan produksi tinggi yang berpotensi sebagai sumber hijauan pakan ternak. Penelitian ini bertujuan untuk mengetahui jenis media tanam terbaik bagi pertumbuhan Indigofera zollingeriana. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan dan 5 ulangan, yaitu tanah (A0), tanah + pasir (A1), tanah + arang sekam (A2), tanah + pupuk kandang (A3), tanah + pasir + pupuk kandang (A4), tanah + arang sekam + pupuk kandang (A5), dan tanah + pasir + arang sekam + pupuk kandang (A6). Data pertumbuhan diantaranya tinggi tanaman, jumlah daun, jumlah cabang dan diameter batang, dianalisis menggunakan analisis ragam (ANOVA) dan jika berpengaruh nyata maka dilakukan uji jarak Duncan Multiple Range Test. Hasil penelitian menunjukkan bahwa tidak ada pengaruh nyata terhadap jenis media tanam terhadap tinggi tanaman, jumlah daun, jumlah cabang, namun berpengaruh nyata terhadap diameter batang Indigofera zollingeriana. Media tanam terbaik terdapat pada perlakuan A5 (tanah + arang sekam + pupuk kandang) terhadap pertumbuhan Indigofera zollingeriana.

Pemberdayaan Masyarakat Nagari Batu Kalang Indah terkait Urgensi Hijauan Pakan Ternak Tinda Afriani; Mangku Mundana; Adisti Rastosari; Moch. Evan Setiawan
Warta Pengabdian Andalas Vol 30 No 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.30.2.321-326.2023

Forage is all food ingredients derived from plants, in the form of leaves, sometimes including stems, twigs, and flowers. Included are grasses, legumes and other plants. All can be fed for ruminants in two forms, i.e. fresh and dry forage. Forages contain almost all the substances animals need. The urgency of forage becomes a crucial point in increasing livestock productivity. The success of a livestock business depends on its feed management. Feed requirements of each animal vary according to the type, age, body weight, environmental conditions and physiological conditions of livestock. Community engagement was carried out with the Batu Kalang Indah cattle group. As many as 20 farmers attended the counselling activity. In addition to socialization activities, 4,000 stalks of grass were also planted, under the name King Grass, using a demonstration method for the groups. The community service activities carried out were responded to positively by the Batu Kalang Indah cattle group.

Karakteristik Kualitatif dan Kuantitatif Induk Sapi Peranakan Onggole (PO) dan Persilangannya di Pacitan, Jawa Timur Adisti Rastosari
Wahana Peternakan Vol. 1 No. 2 (2017): Volume 1 Nomor 2 September 2017
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v1i2.40

ABSTRACT This study aimed to determine the qualitative and quantitative characteristics of Ongole Grade Cow and its crossbreeding in Pacitan, East Java. This study used 105 cattles, which consist of 35 Ongole Grade Cattle, 35 LimPO and 35 SimPO. Ongole Gradeâ€™s coat dominated by white color (100%), coat of LimPO and SimPO dominated by light brown (5.7%) and (17.1%), brown (68.6%) and (57.1%), dark brown (25.7%) both. The muzzle colorâ€™s of Ongole Grade was (100%) black, black (48.6%) and brown (51.4%) for LimPO, black (51.4%) and brown (48.6%) for SimPO. SimPO had a white color on its head. The tail and nail colorâ€™s of Ongole Grade was (100%) black, black (48.6%) and brown (51.4%) for LimPO, black (54.3%) and brown (45.7%) for SimPO. Body sizes consisted of the heart girth, withers height, body length, hip height and head index for Ongole Grade were 160.36Â±9.57 cm; 122.13Â±5.29 cm;113.81Â±8.52 cm;126.31Â±4.77 cm; and 0.41Â±0.05; for LimPO were 167.27Â±14.33 cm;128.59Â±8.02 cm;124.44Â±8.83 cm;131.43Â±8.29 cm; and 0.47Â±0.06; for SimPO were 164.67Â±8.59 cm; 123.97Â±6.91 cm; 126.44Â±9.64 cm; 126.04Â±7.56 cm; and 0.50Â±0.07. From this results, it could be concluded that Ongole Grade, LimPO, and SimPO had different qualitative and quantitative characteristics which SimPO had a white color on its head. Heart girth, withers height, and hip height of LimPO were larger than SimPO and Ongole Grade whereas body length and head index of SimPO were larger than LimPO and Ongole Grade. Key Words: Cow, LimPO, Ongole grade cattle, qualitative, quantitative, SimPO.

Pengaruh Penambahan Bungkil Kedelai terhadap Konsumsi Pakan, Pertambahan Bobot Badan Harian, dan Konversi Pakan pada Sapi Peranakan Simmental Rico; Novi Eka Wati; Adisti Rastosari
Wahana Peternakan Vol. 3 No. 2 (2019): Volume 3 Nomor 2 September 2019
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v3i2.159

The objective of the researh was to study the effect of additions soybean meal in ration on the feed consumption, daily weight gain and feed convertion in Simmental cattle. The experiment used eight male Simmental cattle around 2 years old with an average weight of 198,65 Â± 14,57 kilograms. The cattle kept for 35 days and given rations P0 (rations ranchers) and P1 (rations plus soybean meal). Data was obtained, then analyzed using independent sample t-test assisted with SPSS version 17.00 software. The result showed that the addition of soybean meal did not affect on dry matter consumption, daily weight gain, and feed convertion but, the crude protein consumption was increased. Keywords: dry matter consumption, daily weight gain, feed convertion

Body Condition Score (BCS) Sapi Pesisir Di Kecamatan Lengayang, Kabupaten Pesisir Selatan, Sumatera Barat Adisti Rastosari; Tinda Afriani; James Hellyward; Syahrial Syahrial
Wahana Peternakan Vol. 6 No. 2 (2022): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v6i2.614

Abstract This study aimed to determine the Body Condition Score (BCS) in Pesisir cattle. The Pesisir cattle used were 200 heads, which were located in Lengayang District, Pesisir Selatan Regency, Sumatera Barat Province. Research data was collected using a survey method by direct observations in the field and interviews with farmers. The research variable that was observed was the Body Condition Score (BCS). BCS value was measured using a scale of 1-3 (thin/medium/fat). The proportion of Pesisir cattle with BCS 1, 2, 3 was 14.5%, 70.5% and 15.0%, respectively. The BCS value of Pesisir cattle owned by farmers in the research location was in the normal range which refers to the standard BCS value of 1-3. Â Keyword: Body Condition Score, Pesisir cattle

ANALISIS HUBUNGAN FAKTOR PERSONAL KONSUMEN TERHADAP KEPUTUSAN PEMBELIAN AYAM KAMPUNG DI KOTA PADANG Tevina Edwin; Ida Indrayani; Yolani Utami; Adisti Rastosari
Wahana Peternakan Vol. 7 No. 1 (2023): Wahana Peternakan
Publisher : Fakultas Peternakan Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v7i1.779

This study aims to look at the relationship between personal factors and purchasing decisions for Kampung chicken meat. Because the market is a place for the implementation of the demand and supply processes, it is able to provide products that are in accordance with buyer requests. This research was conducted in Padang city, West Sumatra, to be precise, at the Padang City Market, which is the central market of this area. The research was carried out in September 2022. The sampling technique used was the convenience sampling technique, and the number of samples was 100 people. The research instrument employed closed questionnaires with research variables such as age (X1), education (X2), family size (X3), income (X4), and purchasing decisions (Y). The Spearman rank test was used to analyze data using non-parametric statistical tests. Through the results of the study, it was found that only education had a close relationship with purchasing decisions. Other factors such as age, the number of family members, and income do not have a close relationship with purchasing decisions. The absence of a significant relationship can be influenced by factors outside the factors studied. Keyword : Personality factor, Meat, Kampung Chicken, Padang

Level of Consumer Trust in Online Store in Purchase of Animal Products during The Covid-19 Pandemic in Padang City Aditya Alqamal Alianta; Tevina Edwin; Adisti Rastosari; James Hellyward
JURNAL ILMIAH PETERNAKAN TERPADU Vol 9, No 2 (2021)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v9i2.p194-206

Early in 2020, the world was shocked by the spreading of the Coronavirus from Wuhan China. To mitigate the spread of Covid-19, the Indonesian Government has implemented Large-Scale Social Restrictions (PSBB) throughout Indonesia. PSBB limits the people's movement in Indonesia, including the citizen in Padang city to fulfil their animal protein consumption. The limited movement of the Padang city people has made the citizen begin to purchase animal protein products through online shops. Based on those circumstances, this study aims to measure the level of trust of costumers who fulfil their animal proteins consumption through online stores. The research conducted using a survey method. The respondents were 150 Padang city housewives who were determined by convenience sampling. The research instrument used a questionnaire with a Likert scale. The scores calculated and categorized using a range formula. The results showed that the respondents' trust in the online store was in the high category, with 82.00%. The level of respondents' trust in the urgency of shopping at online stores was in the high category, with a percentage of 73.33%. Level of trust in the reputation and quality of online shop sites was in high category with a percentage of 62.00%. Level of trust in the quality of service provided by online shop sellers was in the high category with a percentage of 70.67%. Level of respondents' trust in making payment transactions is at a high category with a percentage of 84.00%. Level of respondents' in promotions provided by online sellers was in the high category with a percentage of 72.67%. It concluded that a high level of customer trust in the online store will provide satisfaction in purchasing the product, making costumers want to make purchases continuously and turn to be loyal consumers of the online store.

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