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POTENTIAL CHITOSAN OF WASTE SHELL MANTIS SHRIMP (Harpiosquilla raphidea) AS ANTIBACTERIAL Putra, Muhammad Ridho; Karnila, Rahman; Hasan, Bustari
Asian Journal of Aquatic Sciences Vol. 6 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Mantis shrimp carapace waste can be processed into chitin and chitosan as antibacterial. This study aims to determine the characteristics, and heating time of chitosan on the potential of chitosan as an antibacterial, and to obtain the best chitosan in increasing the durability of catfish meatballs. Experimental research method with the manufacture of chitin and continued with deacetylation with a long heating time to obtain chitosan. The analytical parameters consisted of chemical characteristics (moisture content, ash, and degree of deacetylation), the inhibitory power of chitosan against Escherichia coli and Staphylococcus aureus bacteria, and the effectiveness of chitosan on the quality of catfish meatballs. The results showed that the chemical characteristics of chitosan were water content (K1 3.72%, K2 4.65%, K3 5.24%), ash (K1 2.38%, K2 2.53%, K3 3.69%), and the degree of deacetylation (K1 54.31%, K2 78.04%, K3 79.51%). The results of the inhibition test of chitosan against Escherichia coli and Staphylococcus aureus bacteria using the well method showed an inhibition zone with values (K1 10.11 mm and 10.18 mm, K2 11.52 mm and 11.48 mm, K3 12.15 mm and 11. 70 mm, positive control 14.64 mm and 14.02 mm, negative control 9.25 mm and 7.35 mm). This value indicates that the activity of chitosan as an antibacterial is quite strong. The effectiveness of chitosan on the quality of catfish meatballs with 7% chitosan immersion and storage at room temperature showed that the TPC value of catfish meatballs with chitosan could prolong the shelf life of meatballs until day-2.

PEMBERDAYAAN PETANI BUDIDAYA IKAN DI DESA SUNGAI PAKU KABUPATEN KAMPAR MELALUI ALIH TEKNOLOGI PENGOLAHAN MAKANAN JAJANAN BERBASIS SAGU DAN IKAN SEBAGAI USAHA RUMAHAN Dewita, Dewita; Syahrul, Syahrul; Diharmi, Andarini; Karnila, Rahman; Edison, Edison; Sukendi, Sukendi
Journal of Rural and Urban Community Empowerment Vol. 3 No. 1 (2021): Oktober
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

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Salah satu desa potensial sebagai desa pengembangan di kabupaten Kampar Riau adalah Desa Sungai Paku kecamatan Kampar Kiri, merupakan salah satu desa pengembangan di kabupaten Kampar Riau. Di desa Sungai Paku banyak terdapat usaha budidaya ikan di kolam, khususnya ikan patin. Komoditas ikan dan lainnya seperti sagu merupakan komoditas utama, yang termasuk komoditas unggulan daerah berbasis system inovasi daerah (SIDa) Riau. Maka dari itu pengembangan produk makanan jajajan difokuskan pada bahan baku berbasis sagu dan ikan. Kolaborasi produk jajanan berbasis sagu dan ikan dari hasil penelitian sebelumnya menunjukkan bahwa mie sagu yang difortifikasi dengan hasil perikanan mengalami peningkatan nilai gizi protein (dari 0,3% menjadi 5,75%), demikian juga dengan produk jajanan lainnya seperti bakso dan snack ikan. Kegiatan ini bertujuan untuk memberikan alih teknologi pengolahan makanan jajanan dan memberdayakan kaum wanita dari keluarga pembudidaya ikan membuka usaha rumahan makanan jajanan yang banyak diminati masyarakat di daerah tersebut seperti mie sagu ikan, bakso ikan dan snack ikan. Metode penerapan yang digunakan adalah metode survei partisipatif, ceramah, diskusi, praktik langsung, observasi, pendampingan dan evaluasi. Luaran kegiatan adalah : i) Meningkatnya keterampilan peserta dari kelompok mitra tentang pengolahan makanan jajanan berbasis sagu dan ikan (snack , mie dan bako sagu ikan), dan ii) Memberikan solusi pada mitra untuk memulai usaha rumahan produk jajanan berbasis sagu dan ikan.

PELATIHAN PEMANFAATAN ASAM AMINO ESSENSIAL PROTEIN IKAN LELE UNTUK PRODUK OLAHAN ABON IKAN DI DESA MAKMUR KECAMATAN PANGKALAN KERINCI KABUPATEN PELALAWAN RIAU Karnila, Rahman; Sumarto, Sumarto; Sidauruk, Shanty Wisuda
Journal of Rural and Urban Community Empowerment Vol. 3 No. 1 (2021): Oktober
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

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Kelompok masyarakat yang menjadi sasaran pada kegiatan ini adalah kelompok PKK di Desa Makmur Kecamatan Pangkalan Kerinci Kabupaten Pelalawan Riau, memiliki usaha dibidang pengolahan ikan lele menjadi produk “abon” ikan. Permasalahan yang dihadapi oleh kelompok masyarakat ini adalah: 1) Penguasaan teknologi (metode) dalam pengolahan hasil perikanan “abon” ikan dan keterampilam masih sangat rendah dan konvensional dan 2) Kualitas produk “abon” ikan yang dihasilkan masih sangat rendah, meliputi nilai gizi yang belum memadai, terutama kandungan asam amino essensial. Solusi yang ditawarkan untuk pemecahan masalah tersebut adalah: 1) Penerapan ilmu pengetahuan dan teknologi pengolahan bahan baku ikan lele menjadi isolat protein kaya asam amino essensial untuk produk olahan abon ikan dan 2) Penerapan ilmu pengetahuan dan teknologi peningkatan kualitas produk olahan abon ikan, meliputi Pelatihan dan penyuluhan penerapan IPTEK pembuatan tepung dan isolat protein ikan lele serta Pelatihan Penerapan IPTEK pembuatan abon ikan kaya akan asam amino essensial. Metode yang digunakan untuk mencapai solusi dan luaran yang telah ditetapkan di atas, yaitu: 1) Pelatihan dan penyuluhan penerapan IPTEK pembuatan tepung dan isolat protein ikan lele dan 2) Pelatihan Penerapan IPTEK pembuatan “abon” ikan (tongkol) kaya akan asam amino essensial. Hasil pengabdian menunjukkan rendemen daging lumat ikan lele yang dihasilkan adalah 30,54% dengan kandungan protein 19,48%. Sedangkan kadar protein tepung ikan lele adalah 64,20%. Isolat protein yang dihasilkan memiliki kadar protein 80,80% yang didominasi oleh asam amino asam glutamat, asam aspartat, lisin, leusin dan alanin. Secara umum kegiatan pengabdian kepada masyarakat dengan judul “Pelatihan Pemanfaatan Asam Amino Essensial Protein Ikan Lele untuk Produk Olahan Abon Ikan di Desa Makmur Kecamatan Pangkalan Kerinci Kabupaten Pelalawan Riau, Riau” dapat berjalan dengan lancar sesuai dengan yang direncanakan. Peserta mengikuti kegiatan dengan antusias dan terlihat sangat menyadari dan memamahi pentingnya diversifikasi pengolahan hasil perikanan sebagai makanan bernilai gizi tinggi. Peserta dapat menguasai cara pembuatan abon ikan tongkol dengan pengkayaan asam amino essensial dari isolat protein ikan lele yang benar yaitu memahami materi yang disampaikan oleh narasumber.

Identification of Saponins from the Active Fraction Sea Cucumbers (Stichopus vastus sluiter) Bintang, M. Irwan Sapta Purna; Sukmiwati, Mery; Karnila, Rahman
Jurnal Perikanan dan Kelautan Vol. 28 No. 1 (2023): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

This study aims to determine the secondary metabolic content of saponin contained in the active fraction of sea cucumber (Stichopus vastus) by using metabolic test reagents and thin-layer chromatography (TLC) analysis. The active fraction was given the reagent in the form of HCl 2N and strong acid then continued with analysis to determine the Rf value and lieberman-burchad reaction. The results of the metabolic reaction test showed that the active fraction contained steroidal saponin compounds characterized by the presence of a stable foam and the formation of a greenish-green color. TLC analysis produced a single stain with an Rf value of 0.9 and a bluish color reaction after being given Liberman-burchad which confirmed that the saponins contained in the active fraction of sea cucumbers were steroidal saponin.

Potential of Vanname Shrimp Skin Astanxanthin Hydrolyzate as Alpha Glucosidase Enzyme Inhibitor Heriansyah, Ivan; Karnila, Rahman; Buchari, Dewita
Jurnal Perikanan dan Kelautan Vol. 28 No. 3 (2023): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

The purpose of the study was to analyze hydrolysate astaxanthin as an inhibitor of the enzyme alf glucosidase. The method used is an experimental method by applying a Complete Random Design (RAL) The treatment used is a concentration of different papain enzymes, consisting of 3 treatment levels namely t1 (3%), t2 (6%), and t3 (9%). Observations made are 1) proximal analysis; 2) analysis of hydrolysate astaxanthin and its antioxidant activity; 3) analysis of the inhibition test of the enzyme alpha glucosidase and its inhibitory mechanism. The results showed that the proxmat of vanname shrimp skin flour is; water content (9.20% bb), ash (31.46% bk), fat (1.64% bk), protein (37.60% bk), and carbohydrates (29.29% bk) (by different) with a yield of 36.22%. The highest astaxanthin content at the 3% papain enzyme concentration of 3.57 μg/g with the best antioxidant activity has the smallest IC50 value of 88,793 ppm and astaxanthin shows inhibition activity of alpha glucoside enzyme by 49.38% - 58.87% and astaxanthin has a competitive enzyme inhibition mechanism resulting in a decrease in postrandial blood glucose levels.

Edukasi Silvofishery Siput pada Kelompok Pengelola Mangrove di Desa Pangkalan Jambi, Bengkalis Budijono, Budijono; Fauzi, Muhammad; Prianto, Eko; Putra, Ridwan Manda; Karnila, Rahman; Sumiarsih, Eni; Hendrizal, Andri; Efriyeldi, Efriyeldi
Unri Conference Series: Community Engagement Vol 6 (2024): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.6.660-664

The snail silvofishery education in Pangkalan Jambi Village aims to equip mangrove management groups in combining mangrove conservation activities with the utilization of its economic value through the cultivation of Cerithidea obtusa snails, in order to create a balance of mangrove ecosystem services to improve the economy of coastal communities. The target group for this education is the “Harapan Bersama” Mangrove Management Group which is one of the fostered groups of PT. Kilang Pertamina Internasional (PT. KPI). The method used is lectures and tutorials as a result of discussions with the CSR team of PT. KPI. The implementation of education begins with the implementation of a pre-test, presentation of materials accompanied by interactive discussions and post-tests. At the end of the activity, a field review was carried out as a candidate for the silvofishery location. The result is that the implementation of this education is very interactive and gets a positive response from the target group who have long struggled to preserve and utilize the mangrove area through several activities, including planting mangrove seedlings and making abrasion prevention tools and producing mangrove products as well as a mangrove educational tourism village. However, this group does not yet know and understand this silvofishery as a form of environmentally friendly mangrove forest preservation and utilization. Post-activity evaluation has proven that this educational activity has increased the understanding of the target group to diversify their activities in the future. The implementation is to practice and assist the snail silvofishery activities in this group

Identification of Saponins from the Active Fraction Sea Cucumbers (Stichopus vastus sluiter) Bintang, M. Irwan Sapta Purna; Sukmiwati, Mery; Karnila, Rahman
Jurnal Perikanan dan Kelautan Vol. 28 No. 1 (2023): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

This study aims to determine the secondary metabolic content of saponin contained in the active fraction of sea cucumber (Stichopus vastus) by using metabolic test reagents and thin-layer chromatography (TLC) analysis. The active fraction was given the reagent in the form of HCl 2N and strong acid then continued with analysis to determine the Rf value and lieberman-burchad reaction. The results of the metabolic reaction test showed that the active fraction contained steroidal saponin compounds characterized by the presence of a stable foam and the formation of a greenish-green color. TLC analysis produced a single stain with an Rf value of 0.9 and a bluish color reaction after being given Liberman-burchad which confirmed that the saponins contained in the active fraction of sea cucumbers were steroidal saponin.

Potential of Vanname Shrimp Skin Astanxanthin Hydrolyzate as Alpha Glucosidase Enzyme Inhibitor Heriansyah, Ivan; Karnila, Rahman; Buchari, Dewita
Jurnal Perikanan dan Kelautan Vol. 28 No. 3 (2023): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

The purpose of the study was to analyze hydrolysate astaxanthin as an inhibitor of the enzyme alf glucosidase. The method used is an experimental method by applying a Complete Random Design (RAL) The treatment used is a concentration of different papain enzymes, consisting of 3 treatment levels namely t1 (3%), t2 (6%), and t3 (9%). Observations made are 1) proximal analysis; 2) analysis of hydrolysate astaxanthin and its antioxidant activity; 3) analysis of the inhibition test of the enzyme alpha glucosidase and its inhibitory mechanism. The results showed that the proxmat of vanname shrimp skin flour is; water content (9.20% bb), ash (31.46% bk), fat (1.64% bk), protein (37.60% bk), and carbohydrates (29.29% bk) (by different) with a yield of 36.22%. The highest astaxanthin content at the 3% papain enzyme concentration of 3.57 μg/g with the best antioxidant activity has the smallest IC50 value of 88,793 ppm and astaxanthin shows inhibition activity of alpha glucoside enzyme by 49.38% - 58.87% and astaxanthin has a competitive enzyme inhibition mechanism resulting in a decrease in postrandial blood glucose levels.

MINERAL CONTENT PROFILE OF CARAPACE FLOUR, CHITIN, AND CHITOSAN SLIPPER LOBSTER (Thenus orientalis) Putra, Harifa Syah; Hasan, Bustari; Karnila, Rahman; Ghazali, Tengku Muhammad
Asian Journal of Aquatic Sciences Vol. 7 No. 2 (2024): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.7.2.192-199

Slipper lobster live on the bottom of sandy and muddy waters at a depth of 10-200 m and are found throughout Indonesian waters. The carapace is the most significant part of its body, of which approximately 50% is a source of waste, but it can be utilized as functional food in the form of chitin and chitosan. One of the determinations of chitin and chitosan quality depends on the demineralization process. The potential of this shrimp has not been fully utilized due to the lack of information on mineral content after the demineralization process. This study aims to obtain information on flour, chitin, and chitosan mineral content. This research used a descriptive method. The parameters observed were yield, water, ash, and mineral content (K, Mg, Ca, Na, Fe, and P). The results showed that slipper lobster weighed about 80-240 g with a length of 15-25 cm with the yield and mineral content of flour, chitin and chitosan of slipper lobster carapace as follows: yield 29.04%, 41.02%, 33.42%, water 2.36%, 3.96%, 3.25%, ash 52.89%, 8.27%, 3.16%, potassium 55.73 mg/L, 9.09 mg/L, 4.55 mg/L, magnesium 37.23 mg/L, 5.59 mg/L, 5.95 mg/L, calcium 766.87 mg/L, 137.62 mg/L, 126.91 mg/L, sodium 146.62 mg/L, 17.82 mg/L, 10.73 mg/L, iron 12.82 mg/L, 1.89 mg/L, 1.72 mg/L, phosphorus 0.17 mg/L, 0.06 mg/L, 0.05 mg/L.

Potential of Vanname Shrimp Skin Astanxanthin Hydrolyzate as Alpha Glucosidase Enzyme Inhibitor Heriansyah, Ivan; Karnila, Rahman; Buchari, Dewita
Jurnal Perikanan dan Kelautan Vol. 28 No. 3 (2023): October
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

The purpose of the study was to analyze hydrolysate astaxanthin as an inhibitor of the enzyme alf glucosidase. The method used is an experimental method by applying a Complete Random Design (RAL) The treatment used is a concentration of different papain enzymes, consisting of 3 treatment levels namely t1 (3%), t2 (6%), and t3 (9%). Observations made are 1) proximal analysis; 2) analysis of hydrolysate astaxanthin and its antioxidant activity; 3) analysis of the inhibition test of the enzyme alpha glucosidase and its inhibitory mechanism. The results showed that the proxmat of vanname shrimp skin flour is; water content (9.20% bb), ash (31.46% bk), fat (1.64% bk), protein (37.60% bk), and carbohydrates (29.29% bk) (by different) with a yield of 36.22%. The highest astaxanthin content at the 3% papain enzyme concentration of 3.57 μg/g with the best antioxidant activity has the smallest IC50 value of 88,793 ppm and astaxanthin shows inhibition activity of alpha glucoside enzyme by 49.38% - 58.87% and astaxanthin has a competitive enzyme inhibition mechanism resulting in a decrease in postrandial blood glucose levels.

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