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Journal : Gunung Djati Conference Series

Multiplikasi Anggrek (Dendrobium sp var Kumala) Menggunakan Kombinasi Benzyl Amino Purin (BAP) dan Air Kelapa (Cocos nucifera L) Secara In Vitro Nenden Nur Amalia; Ayuni Adawiyah; Ateng Supriyatna
Gunung Djati Conference Series Vol. 6 (2021): Seminar Nasional Biologi (SEMABIO) 6 Tahun 2021
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

Dendrobium sp. is one of the natural orchids in Indonesia. Optimization of media composition for in vitro orchid propagation is needed the multiplication ability and seed quality. The role of PGR in in vitro propagation of orchid plays role in triggering cell division, initiation, shoot growth, and the development of photomorphogenesis. The purpose of this study was to determine the effect of BAP and coconut water on the multiplication of Dendrobium sp. var kumalaorchids, and to determine the optimum concentration of BAP and coconut water on the multiplication of Dendrobium sp. var Kumala. This research was conducted with a single factor Completely Randomized Design (CRD). The treatment tested was the addition of BAP into MS0 media with 3 concentration levels (0,5; 1; 1,5 ppm), combined with 3 concentration levelsof coconut water (100; 150; 200 ml/l), and MS0 medium without the addition of BAP and coconut water (control). Based on the results of the study, it can be seen that there is an effect of BAP concentration and coconut water on the multiplication of Dendrobium sp. var kumalaorchids. The composition of the media with a concentration of 1 ppm BAP combined with 100-150 ml/l coconut water showed optimal results in increasing the number and length of leaves, namely 1,467 ± 0,416 leaves and 0,142 ± 0,036 cm. In contrast to the emergence of orchid leaves, the highest yield was found in 0,5 ppm BAP treatment media combine with 200 ml/l coconut water, with the beginning of the emergence of new leaves occuring at 4 ± 1 DAI. The BAP treatment of 1,5 ppm combined with 200 ml/l coconut water resulted in a higher number of roots, namely 1,22 ± 1,349 pieces.
INDUKSI TUNAS ANGGREK (Dendrobium sp) Var. Kumala MENGGUNAKAN BAP (6-Benzyl Amino Purine) DAN AIR KELAPA SECARA IN-VITRO Reni Annisa Nurhanifah; Ateng Supriyatna; Ayuni Adawiyah
Gunung Djati Conference Series Vol. 6 (2021): Seminar Nasional Biologi (SEMABIO) 6 Tahun 2021
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

Orchid plants are one type of ornamental plant that has a unique flower beauty and a long flower life compared to other flower plants. The beauty and attractiveness of orchids lies in the various shapes and colors of their flowers. because this Dendrobium orchid has the potential as an ornamental plant so that it is widely developed and propagated in Indonesia because the climate is very suitable for growing orchids. This study aimed to determine the effect of BAP (6-Benzyl amino purine) and coconut water on the growth of orchid explants in vitro and to determine the optimum concentration of the combination of BAP and coconut water on the growth of orchid explants in vitro. The method used is the Completely Randomized Design (CRD) method using MS media, there are 10 concentration variations where 1 control concentration and 9 concentrations of BAP and coconut water combination. Each treatment consisted of 3 repetitions. Thus, 30 experimental units were obtained. The results showed that MS media using a combination of 1.5 ppm BAP and 200 ml of coconut water could trigger the growth of leaves, 0.5 ppm BAP treatment plus 150 ml of coconut water could trigger stem elongation and shoot number, while the control treatment can trigger the growth of the number of roots. The survival percentage is 100% where none of the 30 ripening bottles is contaminated.
PENGARUH KONSENTRASI AIR KELAPA (Cocos nucifera L.) TERHADAP EKSPLAN KRISAN (Chrysanthemum morifolium) KULTIVAR ‘XANNE AGRIHORTI’ SECARA IN VITRO Sri Fitri Solihah; Ateng Supriyatna; Ayuni Adawiyah
Gunung Djati Conference Series Vol. 6 (2021): Seminar Nasional Biologi (SEMABIO) 6 Tahun 2021
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

Krisan (Chrysanthemum morifolium) Cultivars ‘Xanne Agrihorti’ is one of the most popular ornamental plants in Indonesia. One of the problems that arise in the cultivation of chrysanthemums is the high cost of growth regulators. Coconut water contains growth regulators in the form of high cytokinins. The purpose of this study was to determine the effect of coconut water on the growth of krisan (Chrysanthemum morifolium) Cultivars ‘Xanne Agrihorti’ in vitro and determine the optimal concentration of coconut water in the growth of chrysanthemum plants in vitro. This study used a Completely Randomized Design (CDR), with concentration of coconut water which consisted of 4 treatment levels : 0 ml/liter, 100 ml/liter, 150 ml/liter, 200ml/liter. The experiment was carried out 3 times, so the experimental units were 12. Parameters measured were number of shoots, number of roots, stem height, root length and survival rate. Data analysis used the Kruskal-Wallis test. The data obtained after being analyzed statistically through the Kruskal-Wallis test at a confidence level of p<0,05. The results showed that the addition of coconut water with a coconut water had an effect on the growth and development of tissue culture with a concentration of 150 ml/L gave optimal results for the formation of the number of leaves as many as 11 leaves, concentration of 100 ml/L gave the best results for the formation of the number of roots as much as 10 roots, and the percentage of survival was 100%.
MOLECULAR DOCKING SENYAWA GOLONGAN FLAVONOID BUAH TIN (Ficus carica L.) TERHADAP ENZIM HIV-1 REVERSE TRANSCRIPTASE Raysha Tryfhatya Nurhaidha; Musa’adah; Ayuni Adawiyah
Gunung Djati Conference Series Vol. 59 (2025): Prosiding Seminar Nasional Biologi (SEMABIO) ke-10 Tahun 2025
Publisher : UIN Sunan Gunung Djati Bandung

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Abstract

HIV (Human Immunodeficiency Virus) merupakan virus yang menyerang sel darah putih terutama sel CD4 (cluster of differentiation 4). Infeksi HIV menyebabkan penurunan kekebalan tubuh, sehingga penderita menjadi rentan terhadap berbagai infeksi oportunistik. Penggunaan obat antiretroviral saat ini dinilai belum sepenuhnya efektif, karena dapat menimbulkan resistensi virus terhadap obat. Kandungan senyawa aktif pada buah tin dilaporkan dapat berfungsi sebagai antivirus. Penelitian ini bertujuan untuk mengidentifikasi senyawa metabolit dari buah tin, berupa senyawa golongan flavonoid yang berpotensi sebagai kandidat antivirus HIV. Penelitian ini dilakukan secara in silico melalui metode molecular docking, dengan menambatkan enam senyawa golongan flavonoid terhadap enzim HIV-1 reverse transcriptase. Penambatan menggunakan sofware PyRx dengan analisis berdasarkan nilai energi afinitas, konstanta inhibisi, dan interaksi molekuler. Hasil menunjukan bahwa keenam senyawa golongan flavonoid pada buah tin berpotensi sebagai inhibitor enzim HIV-1 reverse transcriptase. Dari keenam senyawa tersebut, luteolin merupakan kandidat senyawa paling potensial dalam menghambat enzim HIV-1 reverse transcriptase dengan niai energi afinitas dan konstanta inhibisi terendah (-8,8 kcal/mol dan 0,369 µM) serta memiliki interaksi yang kuat dengan 7 residu asam amino yang berikatan pada sisi aktif enzim HIV-1 reverse transcriptase.