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Antibacterial mechanism of Kratom (Mitragyna speciosa) methanol extract on Streptococcus pneumoniae and Eschericia coli bacteria Hotimah Masdan Salim; Marinda Dwi Puspitarini; Yuani Setiwati; Michio Shimabukuro
Biomolecular and Health Science Journal Vol. 4 No. 2 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i2.28933

Abstract

Introduction: Kratom (Mitragyna speciosa) is a tropical herbal plant native to the North Malay Peninsula, Malaysia and Thailand. In Indonesia, kratom us a typical plant of West Kalimantan, especially in Putussibau. Kratom leaf has many pharmacological effects, one of them is antioxidant. However, the antioxidant and antimicrobial activities of Mitragyna speciosa leaf extracts are lacking. This study was to examine the effect of the methanol extract of Mitragyna speciosa (Rubiaceae Family) leaves on microorganisms Escherichia coli and Strepococcus pneumoniae.Methods: Kratom leaves were extracted with methanol as solvent. In this study, S. pneumoniae and E. coli bacteria have been used. Antibacterial activity tests were carried out at concentrations of 0.78%, 1.56%, 3.125%, 6.25%, 12.5%, 25%, 50% and 100%. Insilico was used to search for secondary metabolites and metabolite interactions in Mytragina speciosa. Results: Mitragyna speciosa leaf methanol extract was effective in inhibiting the growth of E. coli and S. pneumoniae significantly (P<0.001). The growth of e.coli was not identified from a concentration of 25% to 100%. However, there was no bacterial growth in S. pneumoniae from a concentration of 6.25% to 100%. In the insilico study it was found that the secondary metabolite of Mitragyna speciosa is quercetin, which interacts with the ATP1 protein as a predictor in the mechanism of Mitragyna speciosa as an antibacterial. Conclusion: In conclusion, there is no correlation between nutritional status and remission outcome of patients with ALL in the induction phase of therapy. However, high percentage of underweight patients shows nutrition needs special attention to improve therapy outcomes.
Pelatihan Quality Control Pengecatan Gram Untuk Meningkatkan Kemampuan Laboratorium Pada SMK Kesehatan Surabaya Endah Prayekti; Yauwan Tobing Lukiyono; Ary Andini; Thomas Sumarsono; Marinda Dwi Puspitarini; Ibrahim Dwi Waluyo Putra; Husein Firdaus; Devita Rahma Putri
PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT UNIVERSITAS NAHDLATUL ULAMA SURABAYA Vol. 1 No. 1 (2022): Prosiding Seminar Nasional Pengabdian Kepada Masyarakat : Perguruan Tinggi Meng
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (786.385 KB) | DOI: 10.33086/snpm.v1i1.805

Abstract

Pengecatan gram merupakan Teknik rutin yang dilaksanakan pada laboratorium mikrobiologi. Penggunaan Teknik ini dilakukan dalam proses isolasi, identifikasi, hingga pemeliharaan kulturbakteri. Pembacaan hasil pengecatan gram dalam proses identifikasi akan sangat mempengaruhi keberlanjutan proses dan hasil akhir bakteri yang diidentifikasi. Seorang pemeriksa di laboratorium mikrobiologi harus memiliki kemampuan operasional dalam pengecatan gram, melakukan pemantapan mutu, dan memberikan penilaian terhadap penyimpangan pada proses teknis operasional. Jajak pendapat kepada lulusan siswa SMK Kesehatan yang melanjutkan jenjang Pendidikan ke tingkat diploma 4 Analis Kesehatan UNUSA, menunjukkan kurangnya pemahaman tentang Quality Control Pengecatan Gram. Selain itu, keterbatasan pemahaman juga disebabkan akses terhadap pembelajaran laboratorium sangat terbatas saat pandemi covid 19. Metode yang digunakan dalam pengebdian kepada masyarakat ini meliputi pemaparan materi mengenai Quality Control Pengecatan Gram, Praktik Pengecatan Gram di Laboratorium, dan melakukan evaluasi di akhir. Hasil pengamatan di lapangan, siswa yang mengikuti pelatihan pengecatan gram belum banyak terlatih untuk prosedur ini. Hal ini terjadi karena kurangnya pengalaman di laboratorium saat pandemi covid-19. 95,85% peserta menilai kegiatan ini sangat membantu mereka terutama pada sesi praktik di laboratorium. Evaluasi setelah magang menunjukkan bahwa sebanyak 73,9% peserta menjawab adanya manfaat yang diperoleh dari pelatihan untuk pelaksanaan magang. Kegiatan Pengabdian kepada Masyarakat sangat membantu mitra dalam menyiapkan para siswanya untuk dapat memiliki kompetensi yang lebih baik lagi sebelum program magang dilakukan oleh mitra. 65,2% Siswa sebagai partisipan kegiatan ini menilai secara mandiri memiliki kemampuan diatas angka 6 dari maksimalangka 10.
Sosialisasi Herbal Medicine Sebagai Pemberdayaan Potensi Kesehatan Berbasis Pondok Pesantren di Pondok Pesantren Zainul Hasan Genggong Diah Retno Kusumawati Kusumawati; Marinda Dwi Puspitarini; Prima Surya Ardiansyah
PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT UNIVERSITAS NAHDLATUL ULAMA SURABAYA Vol. 1 No. 1 (2022): Prosiding Seminar Nasional Pengabdian Kepada Masyarakat : Perguruan Tinggi Meng
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (817.205 KB) | DOI: 10.33086/snpm.v1i1.847

Abstract

Salah satu potensi Pondok Pesantren yang bisa dimanfaatkan terkait dengan adanya sumber daya dan potensi pembelajaran adalah pemberdayaan potensi kesehatan, utamanya tentang herbal medicine. Mengingat saat ini Covid masih ada, maka herbal medicine diharapkan bisa memberi angin segar untuk upaya terapi pendamping atau upaya peningkatan imunitas. Metode pengabdian masyarakat menggunakan webinar secara online interaktif yang dievaluasi lewat pretest dan posttest. Kegiatan ini dilaksanakan secara daring dengan santri Ponpes Zainul Hasan Genggong. Terlaksananya webinar online mengenai potensi kesehatan berbasis pondok yang diselingi pretest dan posttest serta tanya jawab telah memberikan gambaran yang baik kepada para santri Pondok Pesantren Zainul Hasan Genggong. Peningkatan pengetahuan mengenai herbal medicine diharapkan bisa menjadi titik awal pengembangan potensi kesehatan berbasis pondok pesantren yang selanjutnya bisa terus meningkat dan semakin bermanfaat bagi lingkungan sekitar.
PENERAPAN AJARAN ISLAM DALAM RANGKA MENURUNKAN ANGKA KEJADIAN GASTRITIS DAN GERD DI PONDOK PESANTREN AL-HIKAM BANGKALAN Mustika Chasanatusy Syarifah; Marinda Dwi Puspitarini; Siska Nurul Abidah; Diana Diana Arum Lisnawati; Mufidah Sheena Andani
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 4 No. 5 (2023): Volume 4 Nomor 5 Tahun 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v4i5.21843

Abstract

Permasalahan yang kerap kali dialami oleh santri adalah munculnya keluhan maag atau GERD. Hal ini disebabkan karena berbagai macam faktor, salah satunya adalah pola makan yang tidak teratur dan tingkat stress yang tinggi. Kemudian mitra juga mempunyai jenjang pendidikan MTS dan MA, yang tentu saja ada jenjang tahun akhir dimana siswa harus tekun belajar demi menghadapi ujian masuk perguruan tinggi. Hal diatas tentu bisa menjadi pemicu buruknya pola makan dan pengelolaan stress yang berujung pada penyakit maag atau GERD. Sehingga perlu dilakukan upaya edukasi dengan tujuan meningkatkan pengetahuan santri untuk dapat menerapkan ajaran islam guna menurunkan angka kejadian GERD dan gastritis. Tujuan: Meningkatkan pemahaman santri mengenai cara-cara mandiri untuk menerapkan ajaran islam guna mengurangi angka kejadian GERD dan gastritis. Metode: Metode yang digunakan dalam penyuluhan adalah ceramah dan tanya jawab. Sebelum melakukan penyuluhan dilakukan pre-test, kemudian setelah penyuluhan dilakukan post-test. Hasil pre-test dan post-test dinilai kemudian dilakukan pengolahan data dan evaluasi. Hasil: Hasil uji Wilcoxon menunjukkan nilai p-value 0,000 sehingga nilai sig <0,05 yang menandakan bahwa ada perbedaan signifikan antara data pre-test dan post-test. Perbedaan yang signifikan ini menunjukkan adanya perubahan tingkat pengetahuan pada peserta setelah mengikuti sesi penyuluhan. Kesimpulan: Penyuluhan tentang penerapan ajaran islam dalam rangka menurunkan angka kejadian Gastritis dan GERD di Pondok Pesantren Al-Hikam telah memberikan dampak yang signifikan pada peserta, yaitu berupa meningkatnya tingkat pengetahuan.
Sakura "Periksa Kulit Bersama" Sebagai Deteksi Dini Penyakit Kulit di PP. Al Hikam Bangkalan Maria Ulfa; Marinda Dwi Puspitarini; Wieke Sriwulan; Elly Dwi Masita; Yuriske Agnovianto
PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT Vol. 3 No. 1 (2023): Prosiding Seminar Nasional Pengabdian Kepada Masyarakat : MEMAKSIMALKAN POTENSI
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/snpm.v3i1.1256

Abstract

Latar belakang, Pondok pesantren (ponpes) adalah tempat berkumpulnya banyak orang dimana rentan terjadi penularan penyakit terutama penyakit kulit yaitu skabies. Penyakit skabies mengakibatkan kerusakan pada kulit dan gangguan kenyamanan akibat rasa gatal. Penyakit skabies ini kurang diperhatikan oleh santri di pondok pesantren, faktor penyebabnya adalah lingkungan yang kurang baik, personal hygiene yang buruk, pengetahuan yang kurang sehingga terjadinya risiko penularan penyakit skabies. Oleh karena itu, hal ini menjadi prioritas yang perlu diperhatikan dan dibenahi agar dapat mengurangi risiko penularan penyakit skabies di lingkungan Pondok Pesantren. Tujuan pengabdian masyarakat ini adalah untuk meningkatkan pemahaman dengan memberikan edukasi dan pemeriksaan kulit kepada santri husada agar dapat mengurangi risiko penularan penyakit skabies di lingkungan Pondok Pesantren. Metode, Metode yang digunakan dalam penyuluhan adalah ceramah dan tanya jawab. Sebelum penyuluhan peserta diberi pre-test, kemudian setelah penyuluhan peserta diberi post-test. Hasil, Hasil pre-test dan post-test dinilai kemudian dilakukan pengolahan data dan evaluasi. Hasil pre-test dan post-test mengenai pengetahuan personal hygiene santri dan tanda gejala penyakit kulit skabies melalui kuesioner secara langsung terjadi peningkatan nilai oleh santri yang mengikuti kegiatan ini. Persentase kenaikan nilai rata-rata adalah sebesar 12,24%. Kenaikan nilai rata-rata yang signifikan ini menunjukkan adanya perubahan tingkat pengetahuan pada peserta setelah mengikuti sesi penyuluhan. Kesimpulan, SAKURA "Periksa Kulit Bersama" sebagai deteksi dini Penyakit Kulit di PP. Al Hikam Bangkalan telah meningkatkan pengetahuan dan pemahaman terhadap penyakit kulit.
Pemberdayaan Komunita Lansia: Pendidikan Gizi pada Sekolah Lansia SMART Kota Malang Viantry, Paramita; Sargowo, Djanggan; Puspitarini, Marinda Dwi
ABDIMASKU : JURNAL PENGABDIAN MASYARAKAT Vol 8, No 2 (2025): MEI 2025
Publisher : LPPM UNIVERSITAS DIAN NUSWANTORO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62411/ja.v8i2.2945

Abstract

The nutritional issues faced by the elderly significantly affected the occurrence of non- communicable diseases (degenerative diseases), especially among elderly individuals with obesity. Education and nutrition training played an important role in preventing and managing these conditions through the promotion of balanced nutrition for the elderly. Nutritional education significantly reduced the risk of chronic diseases and increased individual awareness, particularly among the elderly, about the importance of implementing balanced nutrition. The main objective of this community service program was to enhance knowledge about balanced nutrition for the elderly to prevent and manage these conditions at the SMART Elderly School under the Abiyoso Gerontology Foundation in Malang City. The implementation of this community service activity included nutritional education using PowerPoint presentation materials, facilitating Focus Group Discussions (FGDs), conducting anthropometric measurements, and practicing the preparation of balanced nutritional menus for the elderly. Before and after the nutritional education, knowledge assessments regarding balanced nutrition for the elderly were conducted, with paired t-test analysis. The results showed significant data that improved the elderly's understanding of the causes, prevention, and management of non- communicable diseases. Therefore, the importance of regular activities in raising awareness among the elderly about the habit of applying balanced nutrition was highlighted.
Relationship between Santri's Personal Hygiene to the Incidences of Scabies in Islamic Boarding School Maria Ulfa; Dwi Puspitarini, Marinda; Gumilang, Retna; Ideham, Bariah; Agnovianto, Yuriske
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 9 No 1 (2025): Medical Technology and Public Health Journal March 2025
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v9i1.5749

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Background: Skin diseases are often found in Indonesia, such as scabies which is caused by parasites. Scabies causes damage to the skin and discomfort due to itching. This scabies disease is not paid enough attention by students in Islamic boarding schools, the causes are poor environment, poor personal hygiene, lack of knowledge so that there is a risk of transmission of scabies disease. Therefore, this is a priority that needs to be considered and addressed in order to reduce the risk of transmitting scabies in the Islamic boarding school environment. Research objective: to analyze the relationship between the level of personal hygiene knowledge and the incidence of scabies in Islamic boarding school. Method: This type of research is analytical observational survey study with a cross sectional approach. Data collection uses questionnaires. Sampling is total sampling. Data processing and analysis uses chi square analysis. Results: The results of the Chi Square analysis show that the value of p=0.009 (p<0.05), which means that there is a significant relationship between the level of personal hygiene knowledge and the incidence of scabies in students at Islamic boarding schools. Conclusion: The level of knowledge of students about personal hygiene is one of the factors that can influence the transmission of scabies in the Islamic boarding school environment. So the level of knowledge of students must be increased so that it can be a way to prevent the transmission of scabies.
Correlation Between Workload and Burnout of Educational Staff Faculty of Medicine Universitas Nahdlatul Ulama Surabaya Azizah AS, Nur; Puspitarini, Marinda Dwi; Gumilang, Retna; Farikhah, Zumrotul; Nafisah, Irma Binti Roudlotul; Bariyah, Khadijah Khairul
Jurnal Medis Islam Internasional Vol 7 No 1 (2025): June
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/iimj.v7i1.6489

Abstract

Introduction: High workload among educational staff may adversely impact mental health, leading to stress, fatigue, and anxiety. In academic settings, particularly in medical faculties, complex task demands often result in psychological pressure. This study is essential to understand whether workload influences the mental health of educational staff at the Faculty of Medicine Universitas Nahdlatul Ulama Surabaya (FK UNUSA). Objective: This study aims to analyze the relationship between workload and mental health (burnout) among educational staff at the FK UNUSA. Methods: This quantitative study employed a survey approach. Data were collected using standardized questionnaires measuring two main variables: workload (NASA-TLX) and burnout (Maslach Burnout Inventory). A total of 21 educational staff members participated. Statistical analysis was conducted using the Spearman Rank correlation test to examine the relationship between the two variables. Results: The findings indicate a significant correlation between workload and mental health (p = 0.016). Educational staff with higher workloads tended to experience burnout. However, at the FK UNUSA, most educational staff reported mild to moderate workloads, while their mental health conditions were generally categorized as low to moderate burnout. Conclusions: A significant relationship exists between workload and burnout among educational staff at the FK UNUSA (p-value = 0.016). Although workload is an important factor, it does not act alone. Other contributing factors, such as social support, emotional stress, and work environment, also play critical roles in determining burnout levels.
Molecular Genotyping of Tsst-1 Gene Staphylococcus Aureus from Clinical Specimen Puspitarini, Marinda Dwi; Setyarini, Wahyu; Hadi, Usman; Kuntaman, Kuntaman
Folia Medica Indonesiana Vol. 57, No. 2
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Staphylococcus aureus (S. aureus) is the most common pathogen in humans that causes various infections. Variations in infections due to S. aureus are related to the presence of virulence factors, one of which is Toxic Shock Syndrome Toxin-1 (TSST-1) which is the main cause of Toxic Shock Syndrome (TSS). This study aimed to explore the genetic pattern of the tsst-1 genes among MSSA and MRSA from clinical isolates of patients at Dr. Soetomo Academic Hospital, Surabaya-Indonesia. PCR examination was performed on 106 clinical samples of S. aureus isolates to determine the presence of the tsst-1 genes; subsequently, phylogenetic analysis was performed. The positive tsst-1 genes were found in 3 (5.7%) MSSA isolates and 2 (3.8%) MRSA isolates from 106 samples, of which were divided into 53 MSSA isolates and 53 MRSA isolates. No significant difference was noticed between the tsst-1 genes on both the MSSA and MRSA bacteria (p = 0.648). The positive tsst-1 genes were found in 1 (4.5%) of pus specimens and 2 (16.7%) of blood specimens from MSSA isolates. However, the positive tsst-1 genes were found in neither sputum nor urine specimens. The genes were found in 2 (16.7%) of blood specimens from MRSA isolates. Based on the phylogenetic tree, Indonesian tsst-1 isolates belonged to the same clade as Japan, Iran, Iraq, India, Egypt and the United Kingdom isolates. The prevalence of tsst-1 genes of both MSSA and MRSA from clinical isolates in Dr. Soetomo Academic Hospital Surabaya are 5.7% and 3.8%, respectively. The tsst-1 genes of Surabaya-Indonesia, Japan, Iran, Iraq, India, Egypt and the United Kingdom belonged to the same clade.
Santri Care for Tuberculosis: Contextual Education and Early Screening in Islamic Boarding Schools Puspitarini, Marinda Dwi; Donastin, Adyan; Dwiyanu, Paul Agus; Syaikhon, Muhammad; Salsabila, Luhqi Farhana; Said, Muhammad Ilham; Ramadhana, Ain Darojah Siddiq
Indonesia Berdaya Vol 7, No 1 (2026)
Publisher : UKInstitute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.20261377

Abstract

Tuberculosis remains a major public health issue in Indonesia, particularly in densely populated environments such as Islamic boarding schools. This community engagement program aimed to improve awareness, early detection, and prevention of tuberculosis through the “Healthy Adolescents Without TB” campaign at Hidayatulloh Al-Muhajirin Islamic Boarding School, Bangkalan. A total of 150 students participated in the activities, which implemented a participatory, faith-based health education model combining contextual learning, tuberculosis screening, and healthy behavior promotion. Interactive education sessions, audiovisual media, and testimonials from TB survivors significantly increased students’ understanding of disease prevention. Screening identified 13 students (8.6%) with chronic cough, 9 (6.0%) with weight loss, and 5 (3.3%) with night sweats, all referred for further evaluation at local health facilities. The integration of Islamic values into health messages enhanced cultural acceptance and motivated sustainable behavioral change. Overall, the program effectively strengthened adolescent health literacy and fostered active community participation, demonstrating that contextual, participatory, and faith-based education can serve as a replicable model to support Indonesia’s tuberculosis elimination strategy.