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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia The Journal of Experimental Life Sciences (JELS) Media Penelitian dan Pengembangan Kesehatan Biogenesis: Jurnal Ilmiah Biologi Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Journal of Innovation and Applied Technology Research Journal of Life Science Natural B REKAYASA International Journal of Applied Biology JURNAL ENGGANO Jurnal Sumberdaya Akuatik Indopasifik Jurnal Penelitian Pendidikan IPA (JPPIPA) JPM (Jurnal Pemberdayaan Masyarakat) JURNAL ILMIAH PETERNAKAN TERPADU Indonesia Journal of Halal Samakia: Jurnal Ilmu Perikanan Jurnal Kelautan Nasional Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Aquaculture and Fish Health Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Abdi Insani Jurnal Perikanan Pantura Berkala Penelitian Hayati Journal of Agri-Food Science and Technology (JAFoST) JAPI: Jurnal Akses Pengabdian Indonesia Journal of Fisheries & Marine
Asep Awaludin Prihanto
Brawijaya University
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Journal : Journal of Fisheries

 1 
Identifikasi Bakteri Endofit Mangrove Api-Api Putih (Avicennia marina) Penghasil Enzim L-asparaginase [Identification of Mangrove Endophyte Bacteria of Api-Api Putih (Avicennia marina) as Producing L-asparaginase Enzyme] Asep Awaludin Prihanto; Alifatul Fatchiyah; Hartati Kartikaningsih; Ken Audia Pradarameswari
Jurnal Ilmiah Perikanan dan Kelautan Vol. 10 No. 2 (2018): Jurnal ilmiah perikanan dan kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v10i2.10467

Abstract

Enzim L-asparaginase (E.C 3.5.1.1) adalah protein tetramer yang termasuk dalam kelompok keluarga amidohydrolases homolog. ). L-asparaginase secara selektif menghidrolisis L-aspargin (L-Asn) menjadi amonia (NH3) dan asam aspartat. Penelitian ini bertujuan untuk mendapatkan spesies bakteri penghasil enzim L-asparaginase yang diisolasi dari mangrove Avicennia marina yang berasal dari Pantai Bajul Mati, Malang, Jawa Timur, Indonesia. Penelitian ini dilakukan beberapa tahapan yaitu pengambilan dan preparasi sampel, skrining L-asparaginase, dan identifikasi menggunakan uji mycrobact system. Hasil penelitian menunjukkan bahwa isolat dengan aktivitas enzim L-asparaginase terbesar adalah isolat DAM6 yang berasal dari daun Avicennia marina. Isolat DAM6 adalah bakteri merupakan bakteri gram negatif, berbentuk basil, bentuk koloni oval, tepi koloni tidak rata dan elevansi cembung. Berdasarkan analisis microbact,  isolat DAM6 adalah bakteri spesies Pseudomonas aeruginosa.                                               AbstractThe L-asparaginase enzyme (E.C 3.5.1.1) is a tetramer protein belonging to the family group of amidohydrolases. L-asparaginase selectively hydrolyzes L-aspargin (L-Asn) to ammonia (NH3) and aspartic acid. This study aims to obtain species of L-asparaginase enzyme producing bacteria isolated from Avicennia marina mangroves from Bajul Mati Beach, Malang, East Java, Indonesia. This research carried out several stages such as took and prepared samples, screened L-asparaginase, and identified using the mycrobact system test. The results showed that the isolates with the largest L-asparaginase enzyme activity were DAM6 and it was originated from the leaves of Avicennia marina. DAM6 isolate is a gram-negative with an oval shape, uneven edges, and convex elevation. Based on microbact analysis, DAM6 isolates are Pseudomonas aeruginosa.
Identification of Protease-Producing Halophilic Bacteria Isolated from Salt-Pond Soil Asep Awaludin Prihanto; Rahmi Nurdiani; Hidayatun Muyasharoh; Jauharotul Afifah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 12 No. 1 (2020): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v12i1.14725

Abstract

HighlightsThe halophilic bacteria were isolated from salt-pond soil.Isolate C2 was identified as the best protease producer.Isolate C2 was identified as B. amyloliquefaciens strain UBCAbstractProtease is an important enzyme for various industries, such as pharmaceuticals, leather production, meat processing, protein hydrolyzate, food products, and waste processing industries. This study aimed to isolate and identify protease-producing bacteria isolated from salt-pond soil at the Tuban Regency, East Java, Indonesia. Bacterial communities in the soil samples were firstly isolated from the soil samples by culture-dependent technique on Luria Bertani agar enriched with 5% NaCl. Thereafter, bacterial colonies that grew on the media were purified and screened for their protease production using a skim-milk agar. The bacterial colony which produced protease was further identified using phenotypic (gram staining) and genotypic assays (the 16S rDNA sequence). The result showed that one isolate out of six (isolate C2) obtained from the soil sample was observed to produce a protease enzyme. Based on its 16S rDNA sequence, the isolate was identified as Bacillus amyloliquefaciens strain UBC. These results suggest that B. Amyloliquefaciens strain UBC is a salt-tolerant bacterium (halophilic bacteria) which has the potential to be further developed for protease-producing biological agents.
Effect of Aquaculture Wastewater And Zarrouk in Increasing Biomass, Protein, and Carotenoids levels of Spirulina platensis Anisa Millah Taqiyyah; Yenny Risjani; Asep Awaludin Prihanto; Uun Yanuhar; Mohammad Fadjar
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.40822

Abstract

Highlight Research S. platensis has been analyzed for its productivity. Analysis of the productivity of S. platensis on the use of fish culture wastewater media. S. platensis production can be increased by aquaculture wastewater. Aquaculture wastewater enhances the production of cell density, SGR, biomass, protein, and carotenoid of S. platensis. Abstract Increased productivity of Spirulina sp. in the form of high protein, carotenoids, and biomass content can be achieved by improving its nutrient supply. Inorganic fertilizers are nutrient sources, which are generally used in the culture of this organism on laboratory and industrial scale, but there are several drawbacks, including their high costs and limited availability. Several studies have also reported the use of zarrouk fertilizer as a standard culture medium for Spirulina platensis. Therefore, this study aims to determine the effect and the best concentration of fish culture wastewater treatment in Spirulina platensis culture using biomass, protein content, and carotenoid pigments as indicators. A two-factorial completely randomized designs (CRD) was used in this study, where the factors include the dose of organic waste and Zarrouk fertilizer. The microalgae samples, namely S. platensis were cultured using fresh water. This research consists of two factors. The first factor is the dose of organic waste, and the second factor is the dose of Zarrouk fertilizer. The wastewater treatment consisted of 0, 2, 4, and 6 ml/L, while Zarrouk dosages include 0, 0.5, and 1 ml/L. The best results were obtained from the sample treated with 6 ml/L aquaculture wastewater without the addition of Zarrouk. Furthermore, this treatment gave biomass production of 0.781 g/L, 50.441% protein, and 1.246 mg/L carotenoids. Based on the results, S. platensis culture can be carried out using fish culture wastewater without the addition of Zarrouk fertilizer.
Co-Authors Abdul Aziz Jaziri Abu Bakar Sambah Abu Bakar Sambah Aditia Gustiana Gunawan Affan Gaffar Agus Susilo Akhiroh, Puji Alifatul Fatchiyah Andi Kurniawan Andi Kurniawan Anies Chamidah Anies Chamidah Anisa Millah Taqiyyah Anisa Millah Taqiyyah Ardiansyah Kurniawan Beauty Suestining Diyah Dewanti Chawa, Anif Fatwa Choiriyatun Hanifah Dedes Amertaningtyas Djamaludin, Heder Dodyk Pranowo Dwi Febrianti Eddy Suprayitno Euis Asriani Euis Asriani Euis Asriani faidzil Ikram Farida Isroani Farikh Assafri Firman Jaya Gandhatyasri Rohmah, Wendra Gilang Drajat Maulana Guntur Guntur Hanan Dwi Laksono Timur Happy Nursyam Hardoko Hardoko Hardoko Hardoko Hariati, Anik Martinah Herly Evanuarini Hidayatun Muyasharoh Hidayatun Muyasyaroh Hidayatun Muyasyaroh Hidayatun Muyasyaroh Jauharotul Afifah Karimah Karimah Kartika D. Aninta Kartikaningsih, Hartati Ken Audia Pradarameswari Ken Audia Pradarameswari Ken Audia Pradarameswari Khotibul Umam Al Awwaly Linggar Rucitra, Andan M. Aris Munandar Maftuch Maftuch Marlinda Elvina Susanti Masyithoh, Dewi Melianti, Rika Gian Miftakhul Cahyati MOHAMAD FADJAR Mohammad Fadjar Morita Ikasari, Dhita Muhamad Firdaus Muhammad Firdaus Muyasyaroh, Hidayatun Nada Itorul Umam Nurjannah Nurjannah Perdana, Angga Wira Premy Puspitawati Rahayu Putra, Prily Lovian Rahmi Nurdiani Randy Fahrudin Ardiansyah Ria Dewi Andriani Rina Rifqie Mariana Riska Septifani Soffi Trisnaningrum Suci Puspita Sari Suci Puspita Sari Suci Puspita Sari Suci Puspitasari Sukoso Sukoso Suprayogi Suprayogi Tambunan, Jenny Ernawati Titis Sari Kusuma Tri Widayanti, Vindhya Umam, Nada Itorul Wenny Bekti Sunarharum Yanuhar, Uun Yenny Risjani YOGA DWI JATMIKO Yoga Dwi Jatmiko Yunita Eka Puspitasari