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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia The Journal of Experimental Life Sciences (JELS) Media Penelitian dan Pengembangan Kesehatan Biogenesis: Jurnal Ilmiah Biologi Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Journal of Innovation and Applied Technology Research Journal of Life Science Natural B REKAYASA International Journal of Applied Biology JURNAL ENGGANO Jurnal Sumberdaya Akuatik Indopasifik Jurnal Penelitian Pendidikan IPA (JPPIPA) JPM (Jurnal Pemberdayaan Masyarakat) JURNAL ILMIAH PETERNAKAN TERPADU Indonesia Journal of Halal Samakia: Jurnal Ilmu Perikanan Jurnal Kelautan Nasional Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Aquaculture and Fish Health Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Abdi Insani Jurnal Perikanan Pantura Berkala Penelitian Hayati Journal of Agri-Food Science and Technology (JAFoST) JAPI: Jurnal Akses Pengabdian Indonesia Journal of Fisheries & Marine
Asep Awaludin Prihanto
Brawijaya University
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Journal : REKAYASA

 1 
PENGGUNAAN SOFTWARE IMAGE-J UNTUK PENGHITUNGAN DAN VISUALISASI 3D TUTUPAN BIOFILM VIBRIO CHOLERAE EL TOR PADA KONDISI TUMBUH BERBEDA Prihanto, Asep Awaludin
Rekayasa Vol 4, No 2: Oktober 2011
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1488.895 KB) | DOI: 10.21107/rekayasa.v4i2.2335

Abstract

Biofilm adalah sekumpulan mikroorganisme yang menempel dalam suatu permukaan dengan perantara matrik eksoplisakarida. Mikroorganisme dalam bentuk biofilm ternyata menjadi sumber kontaminasi sekunder di dalam produk pangan. Kenyataan ini menjadi latar belakang utama bagi peneliti mikrobiologi pangan untuk mempelajari biofilm. Penelitian biofilm bagi sebagian peneliti sangat identik dengan kerumitan proses penghitungan dan visualisasi penutupan permukaan substrat penempelan bakteri. Penelitian ini ditujukan untuk menghitung dan memvisualisasikan biofilm dengan cara sederhana dan mudah melalui software image-J. Pada penelitian ini beberapa faktor lingkungan seperti pH, suhu, dan kondisi kultur telah diujicobakan untuk mengetahui pengaruhnya terhadap pembentukan biofilm Vibrio Cholerae El Tor. Pembentukan biofilm dihitung berdasarkan angka tutupan (Biofilm Coverage Rate) yang kemudian divisualisasikan. Hasil penelitian menunjukkan bahwa pH, suhu, salinitas dan kondisi kultur mampu memberikan pengaruh yang signifikan terhadap pembentukan biofilm Vibrio Cholerae El Tor. Image-J mampu menghitung dan menggambarkan angka tutupan dengan baik dan dapat dijadikan alternatif software analisis biofilm bakteri. Kata kunci: image-J, biofilm, Vibrio Cholera El Tor AbstractBiofilms are microorganisms that attached to a surface with eksoplisakarida matrix. Microorganisms in a biofilm was known to be a secondary source of contamination in food products. This fact trigger many scientist and food microbiologist conducting research biofilms. Research in biofilm for many researchers are identical with the complexity of the assaying and visualizing surface-covered area of biofilm.. This study was aimed to calculate and visualize biofilms in a simple and easy way through software image-J. In this study several environmental factors such as pH, temperature, and culture conditions have been tested to determine its influence on biofilm formation of Vibrio Cholerae El Tor. Biofilm formation was calculated based on Biofilm Coverage Rate (BCR) and then was visualized. The results showed that pH, temperature, salinity and culture conditions had a significant influence on the formation of Vibrio Cholerae El Tor biofilm. This result illustrated that Image-J can be able to calculate and to precisely describe the BCR visualization, therefore it can be used as alternatives software for bacterial biofilms analysis. Key words: image-J, biofilm, Vibrio Cholera El Tor
Co-Authors Abdul Aziz Jaziri Abu Bakar Sambah Abu Bakar Sambah Aditia Gustiana Gunawan Affan Gaffar Agus Susilo Akhiroh, Puji Alifatul Fatchiyah Andi Kurniawan Andi Kurniawan Angga Wira Perdana Anies Chamidah Anies Chamidah Anif Fatwa Chawa Anisa Millah Taqiyyah Anisa Millah Taqiyyah Ardiansyah Kurniawan Beauty Suestining Diyah Dewanti Choiriyatun Hanifah Dedes Amertaningtyas Djamaludin, Heder Dodyk Pranowo Dwi Febrianti Eddy Suprayitno Euis Asriani Euis Asriani Euis Asriani faidzil Ikram Farida Isroani Farikh Assafri Firman Jaya Gandhatyasri Rohmah, Wendra Gilang Drajat Maulana Guntur Guntur Hanan Dwi Laksono Timur Happy Nursyam Hardoko Hardoko Hardoko Hardoko Herly Evanuarini Hidayatun Muyasharoh Hidayatun Muyasyaroh Hidayatun Muyasyaroh Hidayatun Muyasyaroh Jauharotul Afifah Karimah Karimah Kartika D. Aninta Kartikaningsih, Hartati Ken Audia Pradarameswari Ken Audia Pradarameswari Ken Audia Pradarameswari Khotibul Umam Al Awwaly Linggar Rucitra, Andan M. Aris Munandar Maftuch Maftuch Marlinda Elvina Susanti Masyithoh, Dewi Melianti, Rika Gian Miftakhul Cahyati MOHAMAD FADJAR Mohammad Fadjar Morita Ikasari, Dhita Muhamad Firdaus Muhammad Firdaus Muyasyaroh, Hidayatun Nada Itorul Umam Nurjannah Nurjannah Premy Puspitawati Rahayu Rahmi Nurdiani Randy Fahrudin Ardiansyah Ria Dewi Andriani Rina Rifqie Mariana Riska Septifani Soffi Trisnaningrum Suci Puspita Sari Suci Puspita Sari Suci Puspita Sari Suci Puspitasari Sukoso Sukoso Suprayogi Suprayogi Tambunan, Jenny Ernawati Titis Sari Kusuma Tri Widayanti, Vindhya Umam, Nada Itorul Wenny Bekti Sunarharum Yanuhar, Uun Yenny Risjani Yoga Dwi Jatmiko YOGA DWI JATMIKO Yunita Eka Puspitasari