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All Journal HAYATI Journal of Biosciences Jurnal Bios Logos BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan Natural B Planta Tropika Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Purifikasi Narra J Berkala Penelitian Hayati Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Borneo Journal of Pharmacy Biosaintifika: Journal of Biology & Biology Education Edubiotik : Jurnal Pendidikan, Biologi dan Terapan

Hery Purnobasuki

Departement Of Biology, Faculty Of Science And Technology, Universitas Airlangga.

Author-ID : 349386

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia Dini Ermavitalini; Siska Yulia Rukhmana; Thalita Meidina; Leonardo Pascalis Dimas Cahyo Baskoro; Triono Bagus Saputro; Ni’matuzahroh Ni’matuzahroh; Hery Purnobasuki
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.64882

Microalgae are a group of micro-sized photosynthetic organisms that range from prokaryotic cyanobacteria to eukaryotic algae. Microalgae are widely used as a source of natural food, cosmetic ingredients, food ingredients, and a source of pigments. This study aims to identify species of four microalgae isolates named B1, B2, B3, and S2 from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, and to determine their astaxanthin pigment concentration under 1 M NaCl. Species identification was carried out through a molecular approach by utilization of an 18S rRNA gene marker. A quantitative test of astaxanthin concentration was carried out by spectrophotometric analysis. Molecular identification results show that isolates B1 and B3 are closely related to Chlorella sp., while isolates B2 and S2 are closely related to Picochlorum maculatum. Moreover, under salinity stress condition of 1 M NaCl shown a significant decrement of astaxanthin production compared to the control treatment. At 1 M NaCl, the astaxanthin content of isolate B1 was 4x10-5 mgL-1, isolate B2 was 2x10-5 mgL-1, isolate B3 was 1x10-5 mgL-1, and isolate S2 was 6x10-6 mgL-1. All in all, isolate S2 has the highest astaxanthin among the other isolates at normal conditions, while under salt stress regime, isolate B1 shown to be the best source for astaxanthin.

The Alternative Use Of Water Hyacinth and Reed As Attached Growth Of Microbial In Waste Water Treatment Hery Purnobasuki; Nur Indradewi Oktavitri; Tri Nurhariyati; B. Saadah; Santini Ika Rafsanjani
IPTEK Journal of Proceedings Series Vol 1, No 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2014i1.227

Aquatic plants have ability to decrease of waste water contaminant. The Water Hyacinth (Eichornia crassipes) and Reed (Imperata cylindrical) are aquatic plants which have ability to reduce concentration of BOD, TSS, COD, Total Nitrate and Total Phosphate in wetland. The other side, it can be used for media support in anaerobic treatment. Because of their abilities, there is some possibilities that Water Hyacinth and Reed can be used as attached growth of microorganisms. The aims of this study was to investigate the potential of water hyacinth and reed as attached media of microbe growth. Part of the plants are used is the trunk part. A slices of trunk is 1 cm. The analysis were conducted for 14 days with ratio 60% waste water and 40% air of reactor. Results of this study for water hyacinth that TSS decrease up to 71%. COD and Total Phosphate decrease 60%, Total Nitrate decrease 85%. While, for reed, TSS decrease up to 41%. COD decrease 56%, Total Phosphate decrease 41%. Total Nitrate decrease 10%. In conclusion, both of them had a potential as attached media of microbe growth. But, Water Hyacinth was better as media support than reed.

Kajian Hubungan Konsentrasi Ammonia Dan Chemical Oxygen Demand (COD) Dalam Pengolahan Air Limbah Secara Anaerob Nur Indradewi O; Hery Purnobasuki; Eko Prasetyo K.; Arya Zulfikar P.; Indah Purnamasari; Mega Rosita
Purifikasi Vol 15 No 2 (2015): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25983806.v15.i2.30

Amonia adalah inhibitor dari proses anaerobik. Penelitian ini bertujuan untuk menentukan pengaruh konsentrasi ammonia terhadap penyisihan Chemical Oxygen Demand (COD). Penelitian ini mengkaji air limbah sintetik dengan konsentrasi ammonia 2500 mg/l dan 5000 mg/l. Volume air limbah sintetik dalam reaktor anaerobic adalah 10 liter. Waktu operas ional reaktor adalah 16 hari. Pada air limbah dengan konsentrasi ammonia 2500 mg/l, di hari ke-0 nilai COD sebesar 3816,96 mg/l dan setelah hari ke-16 nilai COD turun menjadi 1023,97 mg/l. Pada air limbah dengan konsentrasi 5000 mg/l, di hari ke-0 nilai COD sebesar 3453,44 mg/l dan setelah hari ke-16 nilai COD turun menjadi 833,467 mg/l. Berdasarkan data tersebut, efisiensi penyisihkan COD pada konsentrasi 2500 mg/l adalah 73,17% dan pada kosentrasi 5000 mg/l adalah 75,86%

PERKEMBANGAN KULTUR DAUN Aglaonema sp. var Siam Pearl, Aglaonema sp. var. Lady Valentin dan Aglaonema sp. var. Lipstik DENGAN PERLAKUAN ZAT PENGATUR TUMBUH IAA DAN BAP Ika Sukma Dewi; Dwi Kusuma Wahyuni; Hery Purnobasuki
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

The aim of this research was to know the effect of 3-indole-acetic acid (IAA) and 6-bensilaminopurine (BAP) and optimum concentration of IAA+BAP toward growth and development of the leaf culture of Aglaonema sp. var. Siam Pearl, Aglaonema sp. var. Lady Valentine, and Aglaonema sp. var. Lipstik. The explants were taken from the leaf. The explants were cultured in solid MS medium with addition various concentration of IAA 0,5 mg/l, 2 mg/l, 3 mg/l, 5 mg/l, and 8 mg/l and BAP 5 mg/l. Observation had been conducted for 16 weeks to know the response the development of explants leaves such as the periphery of explants coloured of brown, leaves explants of arch shaped, the colour of explants to change for pale, and to form callus. The data were include of the number of explants which able to form callus, time of callus formation, the colour and texture callus, and stage of development explants were analyzed descriptively. The result of 16 weeks observation showed that the addition of IAA and BAP on the leaves explants to give the different effects. Aglaonema sp. var. Lipstik can form callus by treatment of 5 mg/l IAA:5mg/l BAP, and 8 mg/l IAA:5 mg BAP. The colour of callus are clear, the shape of globular, with friable textures.

STUDI PENGARUH UMUR DAN TINGGI BATANG TERHADAP STRUKTUR SERAT SIFAT FISIK MORFOLOGI DAN KIMIA BATANG BAMBU APUS Gigantochloa apus Kurz Hery Purnobasuki
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 2 (2001): June 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/491

Characteristic and anatomical study of Gigantochloa apus was carried out to know the fiber structure, morphological, physical, and chemical properties on various age and part of culm height. The research was done by ex post facto design and the data was analyzed by observational description. For anatomical observation, materials were processed using paraffin, PEG, and wood method and maceration for fiber structure. The result showed that inter-nodus amount, diameter, and wall thickness was increase in older culm. Fiber length was 3.8 mm in average, and older culms have longer fiber than the young ones and within the same culm, the lower part had longer fibers than the upper part. The fresh density (0.97-1.17 g/ml) and the oven dry density (0.57-0.79 g/ml) of this bamboo, it was higher for the older culm than younger culm. Liquor sorption (in NaOH 5% solution) into bamboo chips was fairly good. The sorption into the young bamboo was faster and better than into older bamboo. Young bamboo culms contain more water than older culms and the top part within the same culm contain litte water than lower part. The total cellulose in young bamboo culm was higher than the older (64%-54%), but the lignin on the contrary in lower (14%-28%). At the bottom part, the lignin was higher than the upper prt. The ash content was high (1.6%-4.5%) and its fluctuated based on age and the height part of culm.

The Effectiveness of Stingless Bees on Pollination of Bitter Melon Plants Momordica charantia L. (Cucurbitaceae) Andi Gita Maulidyah Indraswari Suhri; RC Hidayat Soesilohadi; Ramadhani Eka Putra; Rika Raffiudin; Hery Purnobasuki; Ali Agus; Sih Kahono
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69124

This study aimed to measure the effectiveness of stingless bee Tetragonula cf. biroi pollination on the fruit formation of bitter melon Momordica charantia plants. We used hoods on the observed bitter melon plants. In the first hood, stingless bees are inserted to help pollinate 100 bitter melon plants, while in the other hoods, stingless bees are not inserted so that there is no assistance in pollinating the other 100 bitter melon plants. The method used is the focal sampling method for 25 days of observation. Based on the results of the study, stingless bee pollination assistance increased the percentage of the number of flowers that became fruit by 390%, the weight of seeds/fruit by 64%, number of seeds/fruit by 260%, fruit weight by 163%, fruit diameter by 91%, and fruit length by 86%. In addition to the size of the fruit, the shape of the bitter melon pollinated by bees is standard (long and straight). In contrast, the bitter melon that does not get pollination assistance grows with a bent shape resembling the letter "C." Bitter melon is an agricultural commodity that needs pollinating agents such as stingless bees because of its monoecy.

Melissopalynology and Vegetation Analysis Surrounding Sunggau of Giant Honey Bee Apis dorsata in Belitung Regency Dwika Bramasta; Ibnul Qayim; Nina Ratna Djuita; Rika Raffiudin; Ramadhani Eka Putra; RC Hidayat Soesilohadi; Hery Purnobasuki
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1167-1174

The forest conversion into oil palm plantations in Belitung impacts the plant source for pollen and nectar to support the honey bee Apis dorsata. This study aimed to identify the plants used by A. dorsata as pollen sources in honey and bee bread in honey bee nests and to analyze the vegetation composition and structure surrounding the sunggau (artificial nesting site) in Belitung Regency. Honey from A. dorsata was collected from bee nests in sunggau on Kampak Island and Tanjung Rusa. The pollen grains from 20 ml honey were acetolysed and counted until 1,200 grains for each honey sample. In Kampak Island, we found eight pollen types in honey dominated by Rhizophora mucronate mangrove pollen and eleven pollen types in bee bread dominated by Melaleuca cajuputi. The pollen type in honey in Tanjung Rusa was similar to those in Kampak Island, and nine pollen types were found in the bee bread dominated by Elaeis guineensis. The vegetation analysis revealed that mangrove and heath forests in Kampak Island were dominated by Lumnitzera littorea and Melaleuca cajuputi, respectively. The results of this study confirm the bees' notable use of the mangrove ecosystem, which adds conservation value, especially in supporting bee management efforts in Belitung.

Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510 Dwi Kusuma Wahyuni; Siti Nur Hafida; Tri Muji Ermayanti; Bambang Prajoga Eko Wardoyo; Hery Purnobasuki; Edy Setiti Wida Utami
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8223

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction.

Optimation of Auxin and Cytokinin on Enhanced Quality and Weight of Coffea liberica Somatic Embryos Fitria Ardiyani; Edy Setiti Wida Utami; Hery Purnobasuki
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 37 No 1 (2021)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v37i1.460

Coffea liberica is a variety of coffee that tolerant to marginal land, especially peatlands. One of propagation methods in C. liberica is somatic embryogenesis(SE) which producing large number of true-to-type plant seedlings in a short time. This research aimed at studying the effect of application of plant growthregulator (PGR) on quality and weight of somatic embryo of C. liberica. Somatic embryo in development stage was induced by Murashige and Skoog medium containing cytokinin as benzyl amino purin (BAP) and auxin as 2,4-dichlorophe-noxyacetic acid (2,4-D). While cotyledonary embryo in germination stage was induced by Murashige and Skoog medium containing cytokinin (BAP) and auxins as 2,4-D, indole acetic acid (IAA) and naphthaleneacetic acid (NAA). The resultsshowed that the application of auxins and cytokinins on development stage affected the formation of embryos, texture of calli, color of calli and embryos, and weight of somatic embryo. It also influenced the shoot and root formation, color and weight of geminating embryos of C. liberica at the germinating stage. During the development stage, addition of 1 mg/L BAP in the absence of 2,4-D in MS medium produced the highest quality of somatic embryo of C. liberica. This medium also produced heaviest somatic embryos but with lighter callus. While in germination stage, all medium treatments produced a typical germinating embryo. Coffea liberica germinating embryo growth optimally on MS medium containing 0.5 mg/L BAP as a single chemical or 0.5 mg/L BAP in combination with 0.5 mg/L IAA for shooting development. Whereas on rooting development, addition of 0.5 mg/L NAA on MS medium produced an optimal germinating embryo. Moreover, germination embryo of C. liberica recorded the highest in terms of dry weight on MS media with addition of 0.5 mg/L BAP. Application of appropriate concentration of auxin and cytokinin is needed to support the formation of somatic embryo and germinating embryo.

Molecular interaction analysis of ferulic acid (4-hydroxy-3-methoxycinnamic acid) as main bioactive compound from palm oil waste against MCF-7 receptors: An in silico study Herdiansyah, Mochammad A.; Rizaldy, Rafli; Alifiansyah, Mochamad RT.; Fetty, Amelia JT.; Anggraini, Dhea; Agustina, Niken; Alfian, Fariz R.; Setianingsih, Primanita NM.; Elfianah, Verah; Aulia, Halimatus S.; Putra, Justitia ERP.; Ansori, Arif NM.; Kharisma, Viol D.; Jakhmola, Vikash; Purnobasuki, Hery; Pratiwi, Intan A.; Rebezov, Maksim; Shmeleva, Svetlana; Bonkalo, Tatyana; Kovalchuk, Dmitriy F.; Zainul, Rahadian
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.775

Ferulic acid (4-hydroxy-3-methoxycinnamic acid) is a phytochemical compound that is commonly found in conjugated forms within mono-, di-, polysaccharides and other organic compounds in cell walls of grain, fruits, and vegetables. This compound is highly abundant in the palm oil waste. The aim of the study was to predict the anticancer activity of ferulic acid against the breast cancer cell lines (MCF-7) receptors through a computational analysis. MCF-7 receptors with PDB IDs of 1R5K, 2IOG, 4IV2, 4IW6, 5DUE, 5T92, and 5U2B were selected based on the SMILE similarity of the native ligand. Thereafter, the protein was prepared on Chimera 1.16 and docked with ferulic acid on Autodock Vina 1.2.5. The ligand-protein complex interaction was validated by computing the root mean square fluctuation (RMSF) and radius of gyration (Rg) through molecular dynamic simulation. In addition, an absorption, distribution, metabolism, excretion, and toxicity (ADMET) prediction was performed on ferulic acid using the pkCSM platform. The molecular docking revealed that the ferulic acid could interact with all receptors as indicated by the affinity energy <-5 kcal/mol. The compound had the most optimum interaction with receptor 2IOG (affinity energy=-6.96 kcal/mol), involving hydrophobic interaction (n=12) and polar hydrogen interaction (n=4). The molecular dynamic simulation revealed that the complex had an RMSF of 1.713 Å with a fluctuation of Rg value around 1.000 Å. The ADMET properties of ferulic acid suggested that the compound is an ideal drug candidate. In conclusion, this study suggested that ferulic acid, which can be isolated from palm oil waste, has the potential to interact with MCF-7 receptors.

Co-Authors Adi Setyo Purnomo Adi Sofyan Ansori, Muhammad Agustina, Niken Ahmad Ridlwan Jamil Aini, Nur Sofiatul Alfian, Fariz R. Ali Agus Alifiansyah, Mochamad RT. Andi Gita Maulidyah Indraswari Suhri Anggraini, Dhea Anika Sindhya Dewi Ansori, Arif NM. Arya Zulfikar P. Aulia, Halimatus S. B. Saadah Bambang Prajoga Bambang Prajoga Eko Wardoyo Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Bezhinar, Tatyana Bonkalo, Tatyana Burkov, Pavel Derkho, Marina Dewangga, Rezha Tanu Dini Ermavitalini Dwi Kusuma Wahyuni Dwika Bramasta Edy Setiti Wida Utami Edy SW Utami, Edy SW Eko Prasetyo K. Eko Prasetyo Kuncoro Elfianah, Verah Farid Kamal Muzaki Fetty, Amelia JT. Fitria Ardiyani Gudz, Petr Hamidah . Herdiansyah, Mochammad A. Herdiansyah, Mochammad Aqilah Hermawan, Endang Husna, Etika Ziadana Al IBNUL QAYIM Ida Kinasih Ika Sukma Dewi Indah Purnamasari Jakhmola, Vikash Jamil, Ahmad Ridlwan Junairiah Junairiah Khairullah, Aswin Rafif Kharisma, Viol D. Kharisma, Viol Dhea Kovalchuk, Dmitriy F. Leonardo Pascalis Dimas Cahyo Baskoro Maksimiuk, Nikolai Mawardani, Alfiyyana Nurrahma Mega Rosita Munawir Sazali Murtadlo, Ahmad Affan Ali Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Nina Ratna Djuita Ni’matuzahroh Ni’matuzahroh Nur Indradewi O Nur Indradewi Oktavitri Pratiwi, Intan A. Purnamasari, Indah Putra, Justitia ERP. Putra, Ramadhani Qonitatun Nisa, Qonitatun R.C. Hidayat Soesilohadi Rahadian Zainul Rahayu , Anisa Esti Rahmahana, Ratna Syifa'a Rahmahana, Ratna Syifa’a Ramadhani Eka Putra RC Hidayat Soesilohadi Rebezov, Maksim RIKA RAFFIUDIN Rizaldy, Rafli Rizam, Bilqis Naura Safira Rofiatun Solekha Rollando, Rollando Santini Ika Rafsanjani Setianingsih, Primanita NM. Shmeleva, Svetlana Sih Kahono Siska Yulia Rukhmana Siti Nur Hafida Siti Nur Hafida, Siti Nur Sucipto Hariyanto Teguh Hari Sucipto, Teguh Hari Thalita Meidina Tri Muji Ermayanti Tri Muji Ermayanti Tri Nurhariyati Triono Bagus Saputro, Triono Bagus Widyananda, Muhammad Hermawan

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