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All Journal HAYATI Journal of Biosciences Jurnal Bios Logos BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan Natural B Indonesian Journal of Tropical and Infectious Disease Planta Tropika Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Purifikasi Narra J Berkala Penelitian Hayati Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Borneo Journal of Pharmacy Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Biodiversitas Journal of Biological Diversity
Hery Purnobasuki
Departement Of Biology, Faculty Of Science And Technology, Universitas Airlangga.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Effects of Plant Media And Fertilization on The Growth of Orchid Plant (dendrobium sylvanum rchb. F.) in Acclimatization Phase Sucipto Hariyanto; Ahmad Ridlwan Jamil; Hery Purnobasuki
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 7, No 1 (2019)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2019.095.66-72

Abstract

This study was to evaluate the effect of plant media variation, time of fertilization, and combination of both on the growth of orchid plant Dendrobium sylvanum Rchb. f. in acclimatization phase. This study was designed using 6x4 factorial experimentation. The first factor was the variation of plant media: charcoals, coconut husk, bagasse, mixing of charcoal and coconut husk, combination of charcoal and bagasse, combination of coconut husk and bagasse. The second factor was fertilization time: no fertilization, 7 day-fertilization, 14 day-fertilization, and 20 day-fertilization for three months. The data were analysed by using Two Way MANOVA (α = 5%) and Duncan test. Based on the results, there were differences between media variation and fertilization time on the increase of leaf length and width, stem diameter, and root length of Dendrobium orchid in acclimatization phase. Combination of bagasse and coconut husk media showed the best result on media treatment. The best of fertilization time was 14 days. Moreover, the best of interaction between media and fertilization time showed by the combination of bagasse and coconut husk with 14 days-fertilization.
Astaxanthin-Producing Microalgae Identification Using 18S rRNA : Isolates from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, East Java, Indonesia Dini Ermavitalini; Siska Yulia Rukhmana; Thalita Meidina; Leonardo Pascalis Dimas Cahyo Baskoro; Triono Bagus Saputro; Ni’matuzahroh Ni’matuzahroh; Hery Purnobasuki
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.64882

Abstract

Microalgae are a group of micro-sized photosynthetic organisms that range from prokaryotic cyanobacteria to eukaryotic algae. Microalgae are widely used as a source  of natural food, cosmetic ingredients, food ingredients, and a source of pigments. This study aims to identify species of four microalgae isolates named B1, B2, B3, and S2 from Bangkalan Mangrove Waters and Sowan Tuban Northern Waters, and to determine their astaxanthin pigment concentration under 1 M NaCl. Species identification was carried out through a molecular approach by utilization of an 18S rRNA gene marker. A quantitative test of astaxanthin concentration was carried out by spectrophotometric analysis. Molecular identification results show that isolates B1 and B3 are closely related to Chlorella sp., while isolates B2 and S2  are closely related to Picochlorum maculatum. Moreover, under salinity stress condition of 1 M NaCl shown a significant decrement of astaxanthin production compared to the control treatment. At 1 M NaCl, the astaxanthin content of isolate B1 was 4x10-5 mgL-1, isolate B2 was 2x10-5 mgL-1, isolate B3 was 1x10-5 mgL-1, and isolate S2 was 6x10-6 mgL-1. All in all, isolate S2 has the highest astaxanthin among the other isolates at normal conditions, while under salt stress regime, isolate B1 shown to be the best source for astaxanthin. 
Performance of Anaerobic Reactor in Attached and Suspended Growth Mechanisms for the Removal of Tofu Wastewater Rosvita Tri Jullyanti; H. Purnobasuki; T. Nurhariyati; F. N. Rachman; N. I. Oktavitri
IPTEK Journal of Proceedings Series Vol 1, No 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2014i1.220

Abstract

This study assessed the removal efficiency of organic compounds in attached and suspended growth mechanisms when an anaerobic reactor was used to treat tofu wastewater. Parameters such as BOD (BiologicalOxygen Demand), TSS (Total Suspended Solid) and VSS (Volatile Suspended Solid) were measured. The assessments of removal efficiency were measuredby the amount of degradation of organic compounds caused by the microorganisms. The analyses were conducted for 14 days with a ratio of 40% air and 60% wastewater for the suspended growth mechanism. In attached growth, gravel served as the media at 30% from 60% of wastewater with 40% air. The removal efficiency of organic compounds measured as the decrease of BOD, TSS, and VSS – in suspended and attached growth. BOD in suspended growth decrease 5,55% while 76,46% in attached growth, TSS in suspended growth decrease 64,59% while increased 19,40% in attached growth, similar results occurred for VSS parameter decrease 2,04% while increased 31,53%. The increased result of TSS and VSS in attached growth caused of biofilm growth. In conclusion, BOD, TSS, and VSS decreased greater when the anaerobic treatment in attached growth was applied.
The Alternative Use Of Water Hyacinth and Reed As Attached Growth Of Microbial In Waste Water Treatment Hery Purnobasuki; Nur Indradewi Oktavitri; Tri Nurhariyati; B. Saadah; Santini Ika Rafsanjani
IPTEK Journal of Proceedings Series Vol 1, No 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2014i1.227

Abstract

Aquatic plants have ability to decrease of waste water contaminant. The Water Hyacinth (Eichornia crassipes) and Reed (Imperata cylindrical) are aquatic plants which have ability to reduce concentration of BOD, TSS, COD, Total Nitrate and Total Phosphate in wetland. The other side, it can be used for media support in anaerobic treatment. Because of their abilities, there is some possibilities that Water Hyacinth and Reed can be used as attached growth of microorganisms. The aims of this study was to investigate the potential of water hyacinth and reed as attached media of microbe growth. Part of the plants are used is the trunk part. A slices of trunk is 1 cm. The analysis were conducted for 14 days with ratio 60% waste water and 40% air of reactor. Results of this study for water hyacinth that TSS decrease up to 71%. COD and Total Phosphate decrease 60%, Total Nitrate decrease 85%. While, for reed, TSS decrease up to 41%. COD decrease 56%, Total Phosphate decrease 41%. Total Nitrate decrease 10%. In conclusion, both of them had a potential as attached media of microbe growth. But, Water Hyacinth was better as media support than reed.
Kajian Hubungan Konsentrasi Ammonia Dan Chemical Oxygen Demand (COD) Dalam Pengolahan Air Limbah Secara Anaerob Nur Indradewi O; Hery Purnobasuki; Eko Prasetyo K.; Arya Zulfikar P.; Indah Purnamasari; Mega Rosita
Purifikasi Vol 15 No 2 (2015): Jurnal Purifikasi
Publisher : Department of Environmental Engineering-Faculty of Civil, Environmental and Geo Engineering. Institut Teknologi Sepuluh Nopember, Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25983806.v15.i2.30

Abstract

Amonia adalah inhibitor dari proses anaerobik. Penelitian ini bertujuan untuk menentukan pengaruh konsentrasi ammonia terhadap penyisihan Chemical Oxygen Demand (COD). Penelitian ini mengkaji air limbah sintetik dengan konsentrasi ammonia 2500 mg/l dan 5000 mg/l. Volume air limbah sintetik dalam reaktor anaerobic adalah 10 liter. Waktu operas ional reaktor adalah 16 hari. Pada air limbah dengan konsentrasi ammonia 2500 mg/l, di hari ke-0 nilai COD sebesar 3816,96 mg/l dan setelah hari ke-16 nilai COD turun menjadi 1023,97 mg/l. Pada air limbah dengan konsentrasi 5000 mg/l, di hari ke-0 nilai COD sebesar 3453,44 mg/l dan setelah hari ke-16 nilai COD turun menjadi 833,467 mg/l. Berdasarkan data tersebut, efisiensi penyisihkan COD pada konsentrasi 2500 mg/l adalah 73,17% dan pada kosentrasi 5000 mg/l adalah 75,86%
PERKEMBANGAN KULTUR DAUN Aglaonema sp. var Siam Pearl, Aglaonema sp. var. Lady Valentin dan Aglaonema sp. var. Lipstik DENGAN PERLAKUAN ZAT PENGATUR TUMBUH IAA DAN BAP Ika Sukma Dewi; Dwi Kusuma Wahyuni; Hery Purnobasuki
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (793.433 KB) | DOI: 10.23869/217

Abstract

The aim of this research was to know the effect of 3-indole-acetic acid (IAA) and 6-bensilaminopurine (BAP) and optimum concentration of IAA+BAP toward growth and development of the leaf culture of Aglaonema sp. var. Siam Pearl, Aglaonema sp. var. Lady Valentine, and Aglaonema sp. var. Lipstik. The explants were taken from the leaf. The explants were cultured in solid MS medium with addition various concentration of IAA 0,5 mg/l, 2 mg/l, 3 mg/l, 5 mg/l, and 8 mg/l and BAP 5 mg/l. Observation had been conducted for 16 weeks to know the response the development of explants leaves such as the periphery of explants coloured of brown, leaves explants of arch shaped, the colour of explants to change for pale, and to form callus. The data were include of the number of explants which able to form callus, time of callus formation, the colour and texture callus, and stage of development explants were analyzed descriptively. The result of 16 weeks observation showed that the addition of IAA and BAP on the leaves explants to give the different effects. Aglaonema sp. var. Lipstik can form callus by treatment of 5 mg/l IAA:5mg/l BAP, and 8 mg/l IAA:5 mg BAP. The colour of callus are clear, the shape of globular, with friable textures.
STUDI PENGARUH UMUR DAN TINGGI BATANG TERHADAP STRUKTUR SERAT SIFAT FISIK MORFOLOGI DAN KIMIA BATANG BAMBU APUS Gigantochloa apus Kurz Hery Purnobasuki
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 2 (2001): June 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/491

Abstract

Characteristic and anatomical study of Gigantochloa apus was carried out to know the fiber structure, morphological, physical, and chemical properties on various age and part of culm height. The research was done by ex post facto design and the data was analyzed by observational description. For anatomical observation, materials were processed using paraffin, PEG, and wood method and maceration for fiber structure. The result showed that inter-nodus amount, diameter, and wall thickness was increase in older culm. Fiber length was 3.8 mm in average, and older culms have longer fiber than the young ones and within the same culm, the lower part had longer fibers than the upper part. The fresh density (0.97-1.17 g/ml) and the oven dry density (0.57-0.79 g/ml) of this bamboo, it was higher for the older culm than younger culm. Liquor sorption (in NaOH 5% solution) into bamboo chips was fairly good. The sorption into the young bamboo was faster and better than into older bamboo. Young bamboo culms contain more water than older culms and the top part within the same culm contain litte water than lower part. The total cellulose in young bamboo culm was higher than the older (64%-54%), but the lignin on the contrary in lower (14%-28%). At the bottom part, the lignin was higher than the upper prt. The ash content was high (1.6%-4.5%) and its fluctuated based on age and the height part of culm.
The Effectiveness of Stingless Bees on Pollination of Bitter Melon Plants Momordica charantia L. (Cucurbitaceae) Andi Gita Maulidyah Indraswari Suhri; RC Hidayat Soesilohadi; Ramadhani Eka Putra; Rika Raffiudin; Hery Purnobasuki; Ali Agus; Sih Kahono
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69124

Abstract

This study aimed to measure the effectiveness of stingless bee Tetragonula cf. biroi pollination on the fruit formation of bitter melon Momordica charantia plants. We used hoods on the observed bitter melon plants. In the first hood, stingless bees are inserted to help pollinate 100 bitter melon plants, while in the other hoods, stingless bees are not inserted so that there is no assistance in pollinating the other 100 bitter melon plants. The method used is the focal sampling method for 25 days of observation. Based on the results of the study, stingless bee pollination assistance increased the percentage of the number of flowers that became fruit by 390%, the weight of seeds/fruit by 64%, number of seeds/fruit by 260%, fruit weight by 163%, fruit diameter by 91%, and fruit length by 86%. In addition to the size of the fruit, the shape of the bitter melon pollinated by bees is standard (long and straight). In contrast, the bitter melon that does not get pollination assistance grows with a bent shape resembling the letter "C." Bitter melon is an agricultural commodity that needs pollinating agents such as stingless bees because of its monoecy. 
Melissopalynology and Vegetation Analysis Surrounding Sunggau of Giant Honey Bee Apis dorsata in Belitung Regency Dwika Bramasta; Ibnul Qayim; Nina Ratna Djuita; Rika Raffiudin; Ramadhani Eka Putra; RC Hidayat Soesilohadi; Hery Purnobasuki
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1167-1174

Abstract

The forest conversion into oil palm plantations in Belitung impacts the plant source for pollen and nectar to support the honey bee Apis dorsata. This study aimed to identify the plants used by A. dorsata as pollen sources in honey and bee bread in honey bee nests and to analyze the vegetation composition and structure surrounding the sunggau (artificial nesting site) in Belitung Regency. Honey from A. dorsata was collected from bee nests in sunggau on Kampak Island and Tanjung Rusa. The pollen grains from 20 ml honey were acetolysed and counted until 1,200 grains for each honey sample. In Kampak Island, we found eight pollen types in honey dominated by Rhizophora mucronate mangrove pollen and eleven pollen types in bee bread dominated by Melaleuca cajuputi. The pollen type in honey in Tanjung Rusa was similar to those in Kampak Island, and nine pollen types were found in the bee bread dominated by Elaeis guineensis. The vegetation analysis revealed that mangrove and heath forests in Kampak Island were dominated by Lumnitzera littorea and Melaleuca cajuputi, respectively. The results of this study confirm the bees' notable use of the mangrove ecosystem, which adds conservation value, especially in supporting bee management efforts in Belitung.
Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510 Dwi Kusuma Wahyuni; Siti Nur Hafida; Tri Muji Ermayanti; Bambang Prajoga Eko Wardoyo; Hery Purnobasuki; Edy Setiti Wida Utami
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8223

Abstract

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be  available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction. 
Co-Authors ACHMAD DJAZULI Adi Setyo Purnomo Adi Sofyan Ansori, Muhammad Agustina, Niken Ahmad Ridlwan Jamil Aini, Nur Sofiatul AISYAH H. RAMADANI Alfian, Fariz R. Alfiyyana Nurrahma Mawardani Ali Agus ALIFIA HAYA AS-SYIFA Alifiansyah, Mochamad RT. Andi Gita Maulidyah Indraswari Suhri Anggraini, Dhea Anika Sindhya Dewi Ansori, Arif NM. Arya Zulfikar P. Aulia, Halimatus S. B. Saadah Bambang Prajoga Bambang Prajoga Eko Wardoyo Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Bezhinar, Tatyana Bonkalo, Tatyana BRUNI FLARANDA YOKU BRUNY FLARANDA YOKU Burkov, Pavel DANI TRI INDRIATI DANI TRI INDRIATI Derkho, Marina Dewangga, Rezha Tanu DINI ERMAVITALINI Dini Ermavitalini Dini Ermavitalini Dio Nurdin Setiawan, Dio Nurdin DWI KUSUMA WAHYUNI DWI KUSUMA WAHYUNI DWI KUSUMA WAHYUNI Dwi Kusuma Wahyuni Dwi Kusuma Wahyuni Dwika Bramasta Edy Setiti Wida Utami Edy Setiti Wida Utami Edy Setiti Wida Utami Edy SW Utami, Edy SW Eko Prasetyo K. Eko Prasetyo Kuncoro Elfianah, Verah ENDANG ARISOESILANINGSIH F. N. Rachman FADHILATUSTSANI SOFIALANA Farid Kamal Muzaki FEBIANTI D. HAPSARI Fetty, Amelia JT. FITRIA ARDIYANI Fitria Ardiyani GALUH AYU RAKASHIWI GALUH AYU RAKASHIWI GALUH AYU RAKHASHIWI GEBBY AGNESSYA ESA OKTAVIA Gudz, Petr GUNAWAN WIBISANA Hamidah . Herdiansyah, Mochammad A. Herdiansyah, Mochammad Aqilah Hermawan, Endang Husna, Etika Ziadana Al IBNUL QAYIM IDA KINASIH Ida Kinasih Ika Sukma Dewi Indah Purnamasari Jakhmola, Vikash Jamil, Ahmad Ridlwan JUNAIRIAH JUNAIRIAH Junairiah Junairiah JUNAIRIAH JUNAIRIAH Khairullah, Aswin Rafif Kharisma, Viol D. Kharisma, Viol Dhea Kholifatul Wanda Putri, Kholifatul Wanda Kovalchuk, Dmitriy F. Leonardo Pascalis Dimas Cahyo Baskoro M. BADRUT TAMAM Mahbubillah, M. Ainul Maksimiuk, Nikolai Mega Rosita Mirzaq Hussein Anwar, Mirzaq Hussein MOCHAMMAD ILHAM MOCHAMMAD ILHAM Munawir Sazali Murtadlo, Ahmad Affan Ali N. I. Oktavitri NASTITI WIJAYANTI NI N. T. PUSPANINGRUM Ni'matuzahroh Ni'matuzahroh Nilna Faizah Fiddarain, Nilna Faizah Nina Ratna Djuita Ni’matuzahroh Ni’matuzahroh Nur Indradewi O Nur Indradewi Oktavitri Pratiwi, Intan A. Prihartini Widiyanti Purnamasari, Indah Putra, Justitia ERP. Putra, Ramadhani PUTUT RAKHMAD PUTUT RAKHMAD PURNAMA PUTUT RAKHMAD PURNAMA Qonitatun Nisa, Qonitatun R.C. Hidayat Soesilohadi Rahadian Zainul Rahayu , Anisa Esti Rahmahana, Ratna Syifa’a RAJIF IRYADI RAMADHANI EKA PUTRA RAMADHANI EKA PUTRA Ramadhani Eka Putra RC HIDAYAT SOESILOHADI RC HIDAYAT SOESSILOHADI RC Hidayat Soesilohadi Rebezov, Maksim RIKA RAFFIUDIN RIKA RAFFIUDIN RIKA RAFFIUDIN Rizaldy, Rafli Rofiatun Solekha Rollando, Rollando Rosvita Tri Jullyanti Santini Ika Rafsanjani SEHANAT PRASONGSUK SEHANAT PRASONGSUK SENJA APRILIA PARAMITA SERAFINAH INDRIYANI Setianingsih, Primanita NM. SHILFIANA RAHAYU Shmeleva, Svetlana Sih Kahono Siska Yulia Rukhmana Siti Nur Hafida Siti Nur Hafida, Siti Nur SITI RIZQIYATUL MUKARROMAH SITI RIZQIYATUL MUKARROMAH Sucipto Hariyanto SUHARYANTO T. Nurhariyati TATIK WARDIYATI Teguh Hari Sucipto, Teguh Hari Thalita Meidina TRI WARSENO Tri Muji Ermayanti Tri Muji Ermayanti Tri Nurhariyati Triono Bagus Saputro TRIONO BAGUS SAPUTRO Triono Bagus Saputro, Triono Bagus Widyananda, Muhammad Hermawan