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All Journal HAYATI Journal of Biosciences Jurnal Bios Logos BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan Natural B Planta Tropika Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Purifikasi Narra J Berkala Penelitian Hayati Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Borneo Journal of Pharmacy Biosaintifika: Journal of Biology & Biology Education Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Hery Purnobasuki
Departement Of Biology, Faculty Of Science And Technology, Universitas Airlangga.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Comparing Effectiveness of Hand Pollination, Wild Insects and Local Stingless Bees (Tetragonula laeviceps) for Pollination of Exotic Mauritius Raspberry (Rubus rosifolius) Putra, Ramadhani; Dewangga, Rezha Tanu; Hermawan, Endang; Kinasih, Ida; Raffiudin, Rika; Soesilohadi, RC Hidayat; Purnobasuki, Hery
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1050-1060

Abstract

Mauritius raspberry (Rubus rosifolius) is an exotic plant cultivated in Indonesia. Studies showed that the productivity of exotic plants is reducing due to a lack of pollination agent for their origin region. Several methods could be applied to overcome this problem. The study aimed to find the most effective method for pollinating this plant among four pollination regimes: Self-pollination, wild insects, stingless bees (Tetragonula laeviceps), and hand-pollination. The observation was conducted on the insect pollinators' activities (visitation rate, flower constancy, and flower handling time), pollination efficiency, and quality of the fruits produced (fruit volume). The results showed a higher visitation rate (10 to 70 per hour), higher flower constancy (visited from 07.00 to 16.00), and longer flower handling time (13.6 s) of T. laeviceps on raspberry flowers than other insects. High activities related to better raspberry pollination success (96%) and bigger fruits produced. Based on this study, applying stingless bees as pollination agents for exotic plants was the best and potentially applicable to other exotic crops.
Identification of Microalgae Isolates Using 18 S rRNA Markers and Testing Their Antioxidant Capacity Ermavitalini, Dini; Rahmahana, Ratna Syifa'a; Saputro, Triono Bagus; Rizam, Bilqis Naura Safira; Purnobasuki, Hery; Ni'matuzahroh
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.19919

Abstract

Microalgae are photoautotrophic microorganisms that synthesize bioactive compounds, including antioxidant compounds. Exploratory research on microalgae with strong antioxidant capacity as free radical scavengers is interesting and important to do. This study aims to identify and evaluate the antioxidant capacity of microalgae. Water samples were diluted with multilevel dilution. Microalgae were isolated using the streak plate method, and four microalgae isolates were successfully cultivated in vitro. Identification was carried out by phylogenetic analysis based on 18S rRNA marker gene sequences, namely Chlorella vulgaris, Desmodesmus armatus, Dictyosphaerium ehrenbergianum, and Vitreochlamys incisa. Antioxidant capacity was evaluated using three methods, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate)), and FRAP (Ferric Reducing Antioxidant Power). IC50 as the concentration required to inhibit 50% of free radicals showed that methanol extract of C. vulgaris had a strong antioxidant capacity in the ABTS test with IC50 of 81.693 ppm and the highest in the DPPH test with IC50 of 297.451 ppm. In the FRAP test, the highest antioxidant capacity was in ethanol extract of D. ehrenbergianum isolate of 74.45 mg AAE/g. The D. armatus isolate had the lowest antioxidant capacity in the ABTS, DPPH, and FRAP tests.
PELATIHAN BUDIDAYA ANGGREK SECARA IN VITRO PADA KELOMPOK TANI ANGGREK “SANDERIANA” Wahyuni, Dwi Kusuma; Utami, Edy Setiti Wida; Hamidah, Hamidah; Purnobasuki, Hery; Junairiah, Junairiah
Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Vol 5 No 1 (2025): Januari
Publisher : Sekolah Tinggi Ilmu Ekonomi Indonesia (STIESIA) Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24034/kreanova.v5i1.6924

Abstract

Indonesia sebagai negara megabiodiversitas mempunyai jenis-jenis anggrek yang melimpah. Pengambilan langsung dari alam membuat banyak jenis anggrek yang masuk dalam daftar CITES (Convention On International Trade In Endangered Species Of Wild Fauna and Flora), Apendiks 1. Salah satu upaya pelestarian anggrek adalah perbanyakan melalui metode kultur jaringan. Departemen Biologi, Fakultas Sains dan Teknologi Universitas Airlangga telah melakukan banyak penelitian tentang budidaya anggrek dengan kultur jaringan. Untuk menyebarluaskan hasil penelitian tersebut agar bermanfaat untuk masyarakat luas, telah dilakukan kegiatan pengabdian masyarakat berupa pelatihan budidaya anggrek untuk kelompok tani anggrek “Sanderiana” di Dadaprejo, Junrejo, Batu, Jawa timur yang berkerjasama dengan DD Orchid dan Taman Husada Graha Famili. Pelatihan difokuskan di Malang karena Malang merupakan sentra kegiatan pertanian anggrek nasional. Pelatihan budidaya nggrek juga difokuskan pada teknik kultur jaringan anggrek untuk anggrek langka sebagai upaya pelestarian dan juga untuk meningkatkan perekonomian petani anggrek. Kegiatan pelatihan dilakukan selama 6 bulan, meliputi tahap persiapan, pelatihan dan workshop, evaluasi 1 dan evalusi 2. Teknik budidaya yang dilakukan adalah budidaya kloning anggrek secara in vitro.
FATTY ACID PROFILES OF MICROALGAE ISOLATES FROM THE WATERS OF ITS DORMITORY POND IN SURABAYA, INDONESIA Mawardani, Alfiyyana Nurrahma; Ermavitalini, Dini; Saputro, Triono Bagus; Muzaki, Farid Kamal; Purnomo, Adi Setyo; Purnobasuki, Hery
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2428

Abstract

ARTICLE HIGHLIGHTS- Microalgae from ITS Dormitory Pond were identified using morphological analysis.- The study analyzed the fatty acid composition of four microalgae genera.- Dictyosphaerium had the highest total fatty acid content.- The findings suggest potential applications of these microalgae in biotechnology.- Further research is needed to improve fatty acid production. ABSTRACT Microalgae are a diverse group of autotrophic microorganisms classified into prokaryotic and eukaryotic groups. Their ability to thrive in various environmental conditions enables the identification of species with distinct fatty acid profiles. The absence of a microalgae culture collection at Sepuluh Nopember Institute of Technology (ITS) motivated the selection of the ITS Dormitory Pond as the research site. This study aimed to identify microalgae species present in the pond through morphological characterization and analyze their fatty acid profiles. Morphological identification was conducted using taxonomic references, while fatty acid composition was determined via GC-MS analysis. The results identified four genera: Dictyosphaerium, Vitreochlamys, Desmodesmus, and Chlorella. Their fatty acid profiles varied, comprising saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA). Dictyosphaerium contained 17.57% SFA, 12.28% MUFA, and 0.33% PUFA, while Vitreochlamys had 10.83% SFA and 10.19% MUFA. Desmodesmus exhibited 14.19% SFA, 7.39% MUFA, and 4.14% PUFA, whereas Chlorella had 1.92% SFA and 2.67% PUFA. These findings highlight the potential of microalgae from ITS Dormitory Pond as a natural source of fatty acids. Further research is recommended to optimize culture conditions for enhancing commercially valuable fatty acid production.
Fatty acid profile analysis of Chlorella vulgaris under salinity and cadmium stress: Feedstock characterization for biodiesel suitability Ermavitalini, Dini; Husna, Etika Ziadana Al; Rahayu , Anisa Esti; Rahmahana, Ratna Syifa’a; Solekha, Rofiatun; Purnobasuki, Hery; Ni’matuzahroh, Ni’matuzahroh
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1261

Abstract

Cultivation of microalgae Chlorella vulgaris under salinity and heavy metal stress can affect the type and quantity of fatty acids in its cells. This study aims to analyze the fatty acid profile of microalgae C. vulgaris InaCCM49 cultivated in media containing 0.6 M NaCl and 95 µM CdCl2 based on the suitability of biodiesel raw material standards. The study began with determining the starter time of microalgae C. vulgaris InaCCM49 culture, cultivation in salinity and cadmium stress medium, determining the growth phase of treatment and control, biomass extraction for metabolomics analysis, UPLC-MS/MS, fatty acid classification using LIPID MAPS Structure Database (LMSD), and analysis of biodiesel quality standards based on ASTM D6751 and EN14214 which include cetane number (CN), saponification value (SV), cold filter plugging point (CFFP), degree of unsaturation (DU), iodine value (IV) and long-chain saturated factor (LCSF). The results showed an increase in the degree of monounsaturated fatty acids (MUFA) by 10.7 % and a decrease in polyunsaturated fatty acids (PUFA) by 36.71% which played a role in the stability of biodiesel compared to the control treatment. The results of the analysis of CN, IV, and CFPP were respectively 55.4, 59, and -16.5. This shows that the composition of fatty acids formed in the microalgae extract C. vulgaris InaCCM49 with salinity and cadmium stress treatment (0.6 M NaCl, 95 µM CdCl2) has the potential to be a source of quality biodiesel raw materials.
Molecular Docking Analysis of Flavonoids from Syzygium cumini (L.) Skeels: Proapoptotic Potential as an Anticancer Mechanism Aini, Nur Sofiatul; Ansori, Arif Nur Muhammad; Widyananda, Muhammad Hermawan; Kharisma, Viol Dhea; Murtadlo, Ahmad Affan Ali; Herdiansyah, Mochammad Aqilah; Rebezov, Maksim; Burkov, Pavel; Gudz, Petr; Derkho, Marina; Bezhinar, Tatyana; Maksimiuk, Nikolai; Sazali, Munawir; Purnobasuki, Hery; Rollando, Rollando; Khairullah, Aswin Rafif; Sucipto, Teguh Hari
Borneo Journal of Pharmacy Vol. 8 No. 3 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i3.9843

Abstract

Non-small cell lung cancer (NSCLC) presents a significant global health challenge, with its prevalence and mortality rates rising steadily. In Indonesia, Syzygium cumini (L.) Skeels, known for its flavonoid richness, has a long history in traditional medicine. However, its specific mechanisms of action against cancer, particularly in inducing apoptosis in NSCLC, have not been fully elucidated. This study utilized an in silico approach to evaluate the pro-apoptotic potential of S. cumini flavonoids against NSCLC by targeting key proteins: Bcl-2, Bax, and Caspase-3. We retrieved flavonoid structures from PubChem and protein data from the Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB). The drug-likeness of these compounds was assessed using Swiss ADME, adhering to Lipinski's rule of five, while their anti-NSCLC probability was predicted using PASS Online. Molecular docking and screening were performed with PyRx, and the results were visualized using Discovery Studio. Our findings identified epigallocatechin 3-O-gallate and ellagic acid as the most promising anti-NSCLC candidates. Ellagic acid demonstrated the strongest binding affinity to Caspase-3, suggesting a potent pro-apoptotic effect. Epigallocatechin 3-O-gallate, on the other hand, exhibited the lowest binding energy across multiple target proteins, particularly Bcl-2 and Bax, indicating its broad pro-apoptotic potential. These results collectively suggest that flavonoids from S. cumini may hold significant promise as a source of novel anti-NSCLC agents, warranting further in vitro and in vivo investigations.
SEED GERMINATION OF Avicennia marina (Forsk.) Vierh. BY PERICARP REMOVAL TREATMENT Purnobasuki, Hery; Utami, Edy Setiti Wida
BIOTROPIA Vol. 23 No. 2 (2016): BIOTROPIA Vol. 23 No. 2 December 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2016.23.2.349

Abstract

Avicennia marina is one species used for building coastal ecosystem stabilization on the Surabaya shores because of its tolerance to high salinity levels and resistance to wave energy. Its existence is threatened by various factors, including land conversion, pollution, and the construction of inter-island bridges. The growth of A. marina is currently limited by seed dormancy. To increase propagation efficiency, seed viability and vigor were compared based on collection dates and pericarp removal methods. The results showed no relationship between the salinity levels of soaking water or seed buoyancy and seed viability or vigor. Collection dates may influence seed viability and vigor, as seeds collected in October and November germinated more quickly than those collected in December, although there were no differences in total germination. Hand-peeled seeds germinated more slowly than soaked seeds for all collection dates, but with no differences in total germination. Seeds collected in October and November grew more vigorously than those collected in December.
Co-Authors Adi Setyo Purnomo Adi Sofyan Ansori, Muhammad Agustina, Niken Ahmad Ridlwan Jamil Aini, Nur Sofiatul Alfian, Fariz R. Ali Agus Alifiansyah, Mochamad RT. Andi Gita Maulidyah Indraswari Suhri Anggraini, Dhea Anika Sindhya Dewi Ansori, Arif NM. Arya Zulfikar P. Aulia, Halimatus S. B. Saadah Bambang Prajoga Bambang Prajoga Eko Wardoyo Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Bezhinar, Tatyana Bonkalo, Tatyana Burkov, Pavel Derkho, Marina Dewangga, Rezha Tanu Dini Ermavitalini Dwi Kusuma Wahyuni Dwika Bramasta Edy Setiti Wida Utami Edy SW Utami, Edy SW Eko Prasetyo K. Eko Prasetyo Kuncoro Elfianah, Verah Farid Kamal Muzaki Fetty, Amelia JT. Fitria Ardiyani Gudz, Petr Hamidah . Herdiansyah, Mochammad A. Herdiansyah, Mochammad Aqilah Hermawan, Endang Husna, Etika Ziadana Al IBNUL QAYIM Ida Kinasih Ika Sukma Dewi Indah Purnamasari Jakhmola, Vikash Jamil, Ahmad Ridlwan Junairiah Junairiah Khairullah, Aswin Rafif Kharisma, Viol D. Kharisma, Viol Dhea Kovalchuk, Dmitriy F. Leonardo Pascalis Dimas Cahyo Baskoro Maksimiuk, Nikolai Mawardani, Alfiyyana Nurrahma Mega Rosita Munawir Sazali Murtadlo, Ahmad Affan Ali Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Nina Ratna Djuita Ni’matuzahroh Ni’matuzahroh Nur Indradewi O Nur Indradewi Oktavitri Pratiwi, Intan A. Purnamasari, Indah Putra, Justitia ERP. Putra, Ramadhani Qonitatun Nisa, Qonitatun R.C. Hidayat Soesilohadi Rahadian Zainul Rahayu , Anisa Esti Rahmahana, Ratna Syifa'a Rahmahana, Ratna Syifa’a Ramadhani Eka Putra RC Hidayat Soesilohadi Rebezov, Maksim RIKA RAFFIUDIN Rizaldy, Rafli Rizam, Bilqis Naura Safira Rofiatun Solekha Rollando, Rollando Santini Ika Rafsanjani Setianingsih, Primanita NM. Shmeleva, Svetlana Sih Kahono Siska Yulia Rukhmana Siti Nur Hafida Siti Nur Hafida, Siti Nur Sucipto Hariyanto Teguh Hari Sucipto, Teguh Hari Thalita Meidina Tri Muji Ermayanti Tri Muji Ermayanti Tri Nurhariyati Triono Bagus Saputro, Triono Bagus Widyananda, Muhammad Hermawan