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Optimation of Auxin and Cytokinin on Enhanced Quality and Weight of Coffea liberica Somatic Embryos Fitria Ardiyani; Edy Setiti Wida Utami; Hery Purnobasuki
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 37 No 1 (2021)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v37i1.460

Abstract

Coffea liberica is a variety of coffee that tolerant to marginal land, especially peatlands. One of propagation methods in C. liberica is somatic embryogenesis(SE) which producing large number of true-to-type plant seedlings in a short time. This research aimed at studying the effect of application of plant growthregulator (PGR) on quality and weight of somatic embryo of C. liberica. Somatic embryo in development stage was induced by Murashige and Skoog medium containing cytokinin as benzyl amino purin (BAP) and auxin as 2,4-dichlorophe-noxyacetic acid (2,4-D). While cotyledonary embryo in germination stage was induced by Murashige and Skoog medium containing cytokinin (BAP) and auxins as 2,4-D, indole acetic acid (IAA) and naphthaleneacetic acid (NAA). The resultsshowed that the application of auxins and cytokinins on development stage affected the formation of embryos, texture of calli, color of calli and embryos, and weight of somatic embryo. It also influenced the shoot and root formation, color and weight of geminating embryos of C. liberica at the germinating stage. During the development stage, addition of 1 mg/L BAP in the absence of 2,4-D in MS medium produced the highest quality of somatic embryo of C. liberica. This medium also produced heaviest somatic embryos but with lighter callus. While in germination stage, all medium treatments produced a typical germinating embryo. Coffea liberica germinating embryo growth optimally on MS medium containing 0.5 mg/L BAP as a single chemical or 0.5 mg/L BAP in combination with 0.5 mg/L IAA for shooting development. Whereas on rooting development, addition of 0.5 mg/L NAA on MS medium produced an optimal germinating embryo. Moreover, germination embryo of C. liberica recorded the highest in terms of dry weight on MS media with addition of 0.5 mg/L BAP. Application of appropriate concentration of auxin and cytokinin is needed to support the formation of somatic embryo and germinating embryo.
COLOSTRUM-COLLAGEN-HYDROXYAPATITE COMPOSITE, AN EXCELLENT CANDIDATE BIOMATERIAL FOR BONE REPAIR AND BONE INFECTION MANAGEMENT Setiawan, Dio Nurdin; Anwar, Mirzaq Hussein; Putri, Kholifatul Wanda; Fiddarain, Nilna Faizah; Widiyanti, Prihartini; Purnobasuki, Heri
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 2 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.779 KB) | DOI: 10.20473/ijtid.v5i2.221

Abstract

In the case of bone fracture or defect after surgery, which is common in patients with bone cancer (osteosarcoma), it takes a long time for closure and it may cause an infection problem. The use of collagen-hydroxyapatite composite with a blend of colostrum as a scaffold is aimed to accelerate the process of osteoblast growth, inhibite the emergence of infections, and act as bone tissue repair material. The method used was the hydrogel formation process and freeze dry process to remove the solvent and to form pores. The composition of scaffold composite manufactured was 15% collagen, 75% hydroxyapatite and 10% colostrum. Combination of scaffold collagen-hydroxyapatite-colostrum has quite reliable properties because SEM test showed that scaffold could bind to both and could bind to both and could form sufficient pores to provide enough place for bone cells (osteoblats) to grow. The results of MTT assay revealed percentage of above 60%, which indicates that the material is not toxic. In conclusion, collagen-hydroxyapatite-colostrum combination is an excellent biomaterial candidate for bone repair and bone infection management.
Molecular interaction analysis of ferulic acid (4-hydroxy-3-methoxycinnamic acid) as main bioactive compound from palm oil waste against MCF-7 receptors: An in silico study Herdiansyah, Mochammad A.; Rizaldy, Rafli; Alifiansyah, Mochamad RT.; Fetty, Amelia JT.; Anggraini, Dhea; Agustina, Niken; Alfian, Fariz R.; Setianingsih, Primanita NM.; Elfianah, Verah; Aulia, Halimatus S.; Putra, Justitia ERP.; Ansori, Arif NM.; Kharisma, Viol D.; Jakhmola, Vikash; Purnobasuki, Hery; Pratiwi, Intan A.; Rebezov, Maksim; Shmeleva, Svetlana; Bonkalo, Tatyana; Kovalchuk, Dmitriy F.; Zainul, Rahadian
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.775

Abstract

Ferulic acid (4-hydroxy-3-methoxycinnamic acid) is a phytochemical compound that is commonly found in conjugated forms within mono-, di-, polysaccharides and other organic compounds in cell walls of grain, fruits, and vegetables. This compound is highly abundant in the palm oil waste. The aim of the study was to predict the anticancer activity of ferulic acid against the breast cancer cell lines (MCF-7) receptors through a computational analysis. MCF-7 receptors with PDB IDs of 1R5K, 2IOG, 4IV2, 4IW6, 5DUE, 5T92, and 5U2B were selected based on the SMILE similarity of the native ligand. Thereafter, the protein was prepared on Chimera 1.16 and docked with ferulic acid on Autodock Vina 1.2.5. The ligand-protein complex interaction was validated by computing the root mean square fluctuation (RMSF) and radius of gyration (Rg) through molecular dynamic simulation. In addition, an absorption, distribution, metabolism, excretion, and toxicity (ADMET) prediction was performed on ferulic acid using the pkCSM platform. The molecular docking revealed that the ferulic acid could interact with all receptors as indicated by the affinity energy <-5 kcal/mol. The compound had the most optimum interaction with receptor 2IOG (affinity energy=-6.96 kcal/mol), involving hydrophobic interaction (n=12) and polar hydrogen interaction (n=4). The molecular dynamic simulation revealed that the complex had an RMSF of 1.713 Å with a fluctuation of Rg value around 1.000 Å. The ADMET properties of ferulic acid suggested that the compound is an ideal drug candidate. In conclusion, this study suggested that ferulic acid, which can be isolated from palm oil waste, has the potential to interact with MCF-7 receptors.
Comparing Effectiveness of Hand Pollination, Wild Insects and Local Stingless Bees (Tetragonula laeviceps) for Pollination of Exotic Mauritius Raspberry (Rubus rosifolius) Putra, Ramadhani; Dewangga, Rezha Tanu; Hermawan, Endang; Kinasih, Ida; Raffiudin, Rika; Soesilohadi, RC Hidayat; Purnobasuki, Hery
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1050-1060

Abstract

Mauritius raspberry (Rubus rosifolius) is an exotic plant cultivated in Indonesia. Studies showed that the productivity of exotic plants is reducing due to a lack of pollination agent for their origin region. Several methods could be applied to overcome this problem. The study aimed to find the most effective method for pollinating this plant among four pollination regimes: Self-pollination, wild insects, stingless bees (Tetragonula laeviceps), and hand-pollination. The observation was conducted on the insect pollinators' activities (visitation rate, flower constancy, and flower handling time), pollination efficiency, and quality of the fruits produced (fruit volume). The results showed a higher visitation rate (10 to 70 per hour), higher flower constancy (visited from 07.00 to 16.00), and longer flower handling time (13.6 s) of T. laeviceps on raspberry flowers than other insects. High activities related to better raspberry pollination success (96%) and bigger fruits produced. Based on this study, applying stingless bees as pollination agents for exotic plants was the best and potentially applicable to other exotic crops.
PELATIHAN BUDIDAYA ANGGREK SECARA IN VITRO PADA KELOMPOK TANI ANGGREK “SANDERIANA” Wahyuni, Dwi Kusuma; Utami, Edy Setiti Wida; Hamidah, Hamidah; Purnobasuki, Hery; Junairiah, Junairiah
Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Vol 5 No 1 (2025): Januari
Publisher : Sekolah Tinggi Ilmu Ekonomi Indonesia (STIESIA) Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24034/kreanova.v5i1.6924

Abstract

Indonesia sebagai negara megabiodiversitas mempunyai jenis-jenis anggrek yang melimpah. Pengambilan langsung dari alam membuat banyak jenis anggrek yang masuk dalam daftar CITES (Convention On International Trade In Endangered Species Of Wild Fauna and Flora), Apendiks 1. Salah satu upaya pelestarian anggrek adalah perbanyakan melalui metode kultur jaringan. Departemen Biologi, Fakultas Sains dan Teknologi Universitas Airlangga telah melakukan banyak penelitian tentang budidaya anggrek dengan kultur jaringan. Untuk menyebarluaskan hasil penelitian tersebut agar bermanfaat untuk masyarakat luas, telah dilakukan kegiatan pengabdian masyarakat berupa pelatihan budidaya anggrek untuk kelompok tani anggrek “Sanderiana” di Dadaprejo, Junrejo, Batu, Jawa timur yang berkerjasama dengan DD Orchid dan Taman Husada Graha Famili. Pelatihan difokuskan di Malang karena Malang merupakan sentra kegiatan pertanian anggrek nasional. Pelatihan budidaya nggrek juga difokuskan pada teknik kultur jaringan anggrek untuk anggrek langka sebagai upaya pelestarian dan juga untuk meningkatkan perekonomian petani anggrek. Kegiatan pelatihan dilakukan selama 6 bulan, meliputi tahap persiapan, pelatihan dan workshop, evaluasi 1 dan evalusi 2. Teknik budidaya yang dilakukan adalah budidaya kloning anggrek secara in vitro.
Fatty acid profile analysis of Chlorella vulgaris under salinity and cadmium stress: Feedstock characterization for biodiesel suitability Ermavitalini, Dini; Husna, Etika Ziadana Al; Rahayu , Anisa Esti; Rahmahana, Ratna Syifa’a; Solekha, Rofiatun; Purnobasuki, Hery; Ni’matuzahroh, Ni’matuzahroh
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1261

Abstract

Cultivation of microalgae Chlorella vulgaris under salinity and heavy metal stress can affect the type and quantity of fatty acids in its cells. This study aims to analyze the fatty acid profile of microalgae C. vulgaris InaCCM49 cultivated in media containing 0.6 M NaCl and 95 µM CdCl2 based on the suitability of biodiesel raw material standards. The study began with determining the starter time of microalgae C. vulgaris InaCCM49 culture, cultivation in salinity and cadmium stress medium, determining the growth phase of treatment and control, biomass extraction for metabolomics analysis, UPLC-MS/MS, fatty acid classification using LIPID MAPS Structure Database (LMSD), and analysis of biodiesel quality standards based on ASTM D6751 and EN14214 which include cetane number (CN), saponification value (SV), cold filter plugging point (CFFP), degree of unsaturation (DU), iodine value (IV) and long-chain saturated factor (LCSF). The results showed an increase in the degree of monounsaturated fatty acids (MUFA) by 10.7 % and a decrease in polyunsaturated fatty acids (PUFA) by 36.71% which played a role in the stability of biodiesel compared to the control treatment. The results of the analysis of CN, IV, and CFPP were respectively 55.4, 59, and -16.5. This shows that the composition of fatty acids formed in the microalgae extract C. vulgaris InaCCM49 with salinity and cadmium stress treatment (0.6 M NaCl, 95 µM CdCl2) has the potential to be a source of quality biodiesel raw materials.
Molecular Docking Analysis of Flavonoids from Syzygium cumini (L.) Skeels: Proapoptotic Potential as an Anticancer Mechanism Aini, Nur Sofiatul; Ansori, Arif Nur Muhammad; Widyananda, Muhammad Hermawan; Kharisma, Viol Dhea; Murtadlo, Ahmad Affan Ali; Herdiansyah, Mochammad Aqilah; Rebezov, Maksim; Burkov, Pavel; Gudz, Petr; Derkho, Marina; Bezhinar, Tatyana; Maksimiuk, Nikolai; Sazali, Munawir; Purnobasuki, Hery; Rollando, Rollando; Khairullah, Aswin Rafif; Sucipto, Teguh Hari
Borneo Journal of Pharmacy Vol. 8 No. 3 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i3.9843

Abstract

Non-small cell lung cancer (NSCLC) presents a significant global health challenge, with its prevalence and mortality rates rising steadily. In Indonesia, Syzygium cumini (L.) Skeels, known for its flavonoid richness, has a long history in traditional medicine. However, its specific mechanisms of action against cancer, particularly in inducing apoptosis in NSCLC, have not been fully elucidated. This study utilized an in silico approach to evaluate the pro-apoptotic potential of S. cumini flavonoids against NSCLC by targeting key proteins: Bcl-2, Bax, and Caspase-3. We retrieved flavonoid structures from PubChem and protein data from the Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB). The drug-likeness of these compounds was assessed using Swiss ADME, adhering to Lipinski's rule of five, while their anti-NSCLC probability was predicted using PASS Online. Molecular docking and screening were performed with PyRx, and the results were visualized using Discovery Studio. Our findings identified epigallocatechin 3-O-gallate and ellagic acid as the most promising anti-NSCLC candidates. Ellagic acid demonstrated the strongest binding affinity to Caspase-3, suggesting a potent pro-apoptotic effect. Epigallocatechin 3-O-gallate, on the other hand, exhibited the lowest binding energy across multiple target proteins, particularly Bcl-2 and Bax, indicating its broad pro-apoptotic potential. These results collectively suggest that flavonoids from S. cumini may hold significant promise as a source of novel anti-NSCLC agents, warranting further in vitro and in vivo investigations.
SEED GERMINATION OF Avicennia marina (Forsk.) Vierh. BY PERICARP REMOVAL TREATMENT Hery Purnobasuki; Edy Setiti Wida Utami
BIOTROPIA Vol. 23 No. 2 (2016): BIOTROPIA Vol. 23 No. 2 December 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2016.23.2.349

Abstract

Avicennia marina is one species used for building coastal ecosystem stabilization on the Surabaya shores because of its tolerance to high salinity levels and resistance to wave energy. Its existence is threatened by various factors, including land conversion, pollution, and the construction of inter-island bridges. The growth of A. marina is currently limited by seed dormancy. To increase propagation efficiency, seed viability and vigor were compared based on collection dates and pericarp removal methods. The results showed no relationship between the salinity levels of soaking water or seed buoyancy and seed viability or vigor. Collection dates may influence seed viability and vigor, as seeds collected in October and November germinated more quickly than those collected in December, although there were no differences in total germination. Hand-peeled seeds germinated more slowly than soaked seeds for all collection dates, but with no differences in total germination. Seeds collected in October and November grew more vigorously than those collected in December.
FATTY ACID PROFILES OF MICROALGAE ISOLATES FROM THE WATERS OF ITS DORMITORY POND IN SURABAYA, INDONESIA Alfiyyana Nurrahma Mawardani; Dini Ermavitalini; Triono Bagus Saputro; Farid Kamal Muzaki; Adi Setyo Purnomo; Hery Purnobasuki
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2428

Abstract

ARTICLE HIGHLIGHTS- Microalgae from ITS Dormitory Pond were identified using morphological analysis.- The study analyzed the fatty acid composition of four microalgae genera.- Dictyosphaerium had the highest total fatty acid content.- The findings suggest potential applications of these microalgae in biotechnology.- Further research is needed to improve fatty acid production. ABSTRACT Microalgae are a diverse group of autotrophic microorganisms classified into prokaryotic and eukaryotic groups. Their ability to thrive in various environmental conditions enables the identification of species with distinct fatty acid profiles. The absence of a microalgae culture collection at Sepuluh Nopember Institute of Technology (ITS) motivated the selection of the ITS Dormitory Pond as the research site. This study aimed to identify microalgae species present in the pond through morphological characterization and analyze their fatty acid profiles. Morphological identification was conducted using taxonomic references, while fatty acid composition was determined via GC-MS analysis. The results identified four genera: Dictyosphaerium, Vitreochlamys, Desmodesmus, and Chlorella. Their fatty acid profiles varied, comprising saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA). Dictyosphaerium contained 17.57% SFA, 12.28% MUFA, and 0.33% PUFA, while Vitreochlamys had 10.83% SFA and 10.19% MUFA. Desmodesmus exhibited 14.19% SFA, 7.39% MUFA, and 4.14% PUFA, whereas Chlorella had 1.92% SFA and 2.67% PUFA. These findings highlight the potential of microalgae from ITS Dormitory Pond as a natural source of fatty acids. Further research is recommended to optimize culture conditions for enhancing commercially valuable fatty acid production.
A model of relationship between climate and soil factors related to oxalate content in porang (Amorphophallus muelleri Blume) corm SERAFINAH INDRIYANI; ENDANG ARISOESILANINGSIH; TATIK WARDIYATI; HERY PURNOBASUKI
Biodiversitas Journal of Biological Diversity Vol. 12 No. 1 (2011)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d120109

Abstract

Indriyani S, Arisoesilaningsih E, Wardiyati T, Purnobasuki H (2011) A model of relationship between climate and soil factors related to oxalate content in porang (Amorphophallus muelleri Blume) corm. Biodiversitas 12: 45-51. The abiotic environment as well as the biotic environment, involved climate and soil affect directly or indirectly to plant growth as well as plant substance. The objective of the research was to obtain a model of relationship between climate and soil factors related to oxalate content in porang corm. Porang corms were collected from five locations of porang agroforestry in East Java. The locations were (i) Klangon Village, Saradan Subdistrict, Madiun District; (ii) Klino Villlage, Sekar Subdistrict, Bojonegoro District; (iii) Bendoasri Village, Rejoso Subdistrict, Nganjuk District;(iv) Sugihwaras Village, Nggluyu Subdistrict, Nganjuk District and (v) Kalirejo Village, Kalipare Subdistrict, Malang District.Geography variable consist of altitude. Climate variables consist of percentage of radiation, temperature and rainfall. Soil variables consist of electrical conductivity, pH, soil specific gravity, soil organic matter, available of calcium, and cation exchange capacity (CEC). Vegetation variables consist of species of plant tree and percentage of coverage. Porang vegetative growth variables consist of plant height, number of bulbil, canopy diameter, and petiole diameter. Corm variables consist of corm diameter, corm weight, and corm specific gravity. Oxalate variables consist of total oxalate, soluble oxalate, insoluble oxalate, and density of calcium oxalate crystal.Oxalate contents were measured based on AOAC method. All of variables were collected from first to fourth growth period of porang. Data were analyzed by smartPLS (Partial Least Square) software. The results showed that there were significantly direct effect between altitude and temperature, altitude and CEC of soil, temperature and CEC of soil, altitude and percentage of coverage, temperature and percentage of coverage, CEC of soil and percentage of coverage, CEC of soil and petiole diameter, petiole diameter and corm diameter, and petiole diameter and corm oxalate content. There were no significantly direct effect among altitude, temperature, percentage of coverage and petiole diameter; and among corm total oxalate, soluble oxalate, insoluble oxalate, and density of calcium oxalate crystal and corm diameter. The value of Goodness of Fit of the developing model was R²=0.99.Key words: corm, climate, model, oxalate, smartPLS, porang, soil.
Co-Authors ACHMAD DJAZULI Adi Setyo Purnomo Adi Sofyan Ansori, Muhammad Agustina, Niken Ahmad Ridlwan Jamil Aini, Nur Sofiatul AISYAH H. RAMADANI Alfian, Fariz R. Alfiyyana Nurrahma Mawardani Ali Agus ALIFIA HAYA AS-SYIFA Alifiansyah, Mochamad RT. Andi Gita Maulidyah Indraswari Suhri Anggraini, Dhea Anika Sindhya Dewi Ansori, Arif NM. Arya Zulfikar P. Aulia, Halimatus S. B. Saadah Bambang Prajoga Bambang Prajoga Eko Wardoyo Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Bezhinar, Tatyana Bonkalo, Tatyana BRUNI FLARANDA YOKU BRUNY FLARANDA YOKU Burkov, Pavel DANI TRI INDRIATI DANI TRI INDRIATI Derkho, Marina Dewangga, Rezha Tanu DINI ERMAVITALINI Dini Ermavitalini Dini Ermavitalini Dio Nurdin Setiawan, Dio Nurdin DWI KUSUMA WAHYUNI DWI KUSUMA WAHYUNI DWI KUSUMA WAHYUNI Dwi Kusuma Wahyuni Dwi Kusuma Wahyuni Dwika Bramasta Edy Setiti Wida Utami Edy Setiti Wida Utami Edy Setiti Wida Utami Edy SW Utami, Edy SW Eko Prasetyo K. Eko Prasetyo Kuncoro Elfianah, Verah ENDANG ARISOESILANINGSIH F. N. Rachman FADHILATUSTSANI SOFIALANA Farid Kamal Muzaki FEBIANTI D. HAPSARI Fetty, Amelia JT. FITRIA ARDIYANI Fitria Ardiyani GALUH AYU RAKASHIWI GALUH AYU RAKASHIWI GALUH AYU RAKHASHIWI GEBBY AGNESSYA ESA OKTAVIA Gudz, Petr GUNAWAN WIBISANA Hamidah . Herdiansyah, Mochammad A. Herdiansyah, Mochammad Aqilah Hermawan, Endang Husna, Etika Ziadana Al IBNUL QAYIM IDA KINASIH Ida Kinasih Ika Sukma Dewi Indah Purnamasari Jakhmola, Vikash Jamil, Ahmad Ridlwan JUNAIRIAH JUNAIRIAH JUNAIRIAH JUNAIRIAH Junairiah Junairiah Khairullah, Aswin Rafif Kharisma, Viol D. Kharisma, Viol Dhea Kholifatul Wanda Putri, Kholifatul Wanda Kovalchuk, Dmitriy F. Leonardo Pascalis Dimas Cahyo Baskoro M. BADRUT TAMAM Mahbubillah, M. Ainul Maksimiuk, Nikolai Mega Rosita Mirzaq Hussein Anwar, Mirzaq Hussein MOCHAMMAD ILHAM MOCHAMMAD ILHAM Munawir Sazali Murtadlo, Ahmad Affan Ali N. I. Oktavitri NASTITI WIJAYANTI NI N. T. PUSPANINGRUM Ni'matuzahroh Ni'matuzahroh Nilna Faizah Fiddarain, Nilna Faizah Nina Ratna Djuita Ni’matuzahroh Ni’matuzahroh Nur Indradewi O Nur Indradewi Oktavitri Pratiwi, Intan A. Prihartini Widiyanti Purnamasari, Indah Putra, Justitia ERP. Putra, Ramadhani PUTUT RAKHMAD PUTUT RAKHMAD PURNAMA PUTUT RAKHMAD PURNAMA Qonitatun Nisa, Qonitatun R.C. Hidayat Soesilohadi Rahadian Zainul Rahayu , Anisa Esti Rahmahana, Ratna Syifa’a RAJIF IRYADI RAMADHANI EKA PUTRA RAMADHANI EKA PUTRA Ramadhani Eka Putra RC HIDAYAT SOESILOHADI RC HIDAYAT SOESSILOHADI RC Hidayat Soesilohadi Rebezov, Maksim RIKA RAFFIUDIN RIKA RAFFIUDIN RIKA RAFFIUDIN Rizaldy, Rafli Rofiatun Solekha Rollando, Rollando Rosvita Tri Jullyanti Santini Ika Rafsanjani SEHANAT PRASONGSUK SEHANAT PRASONGSUK SENJA APRILIA PARAMITA SERAFINAH INDRIYANI Setianingsih, Primanita NM. SHILFIANA RAHAYU Shmeleva, Svetlana Sih Kahono Siska Yulia Rukhmana Siti Nur Hafida Siti Nur Hafida, Siti Nur SITI RIZQIYATUL MUKARROMAH SITI RIZQIYATUL MUKARROMAH Sucipto Hariyanto SUHARYANTO T. Nurhariyati TATIK WARDIYATI Teguh Hari Sucipto, Teguh Hari Thalita Meidina TRI WARSENO Tri Muji Ermayanti Tri Muji Ermayanti Tri Nurhariyati Triono Bagus Saputro TRIONO BAGUS SAPUTRO Triono Bagus Saputro, Triono Bagus Widyananda, Muhammad Hermawan