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All Journal ACTA VETERINARIA INDONESIANA Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary
Hana Eliyani
Department Of Veterinary Anatomy, Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : journal of Basic Medical Veterinary

 1 
PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL SERTOLI MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Berlina, Cyrcilia Relita; Eliyani, Hana; Sami, Abdul; Widjiati, Widjiati; Mulyati, Sri; Anwar, Chairul
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19991

Abstract

This study  was aimed  to know  the effect of Kebar grass  extract  on amount of mice  Sertoli  cell exposed  by TCDD.  This study was  experimental   study  with completely   randomized  design.  Thirty adult  male  mice  of Mus  musculus strain Balb/C,  age 11 weeks  and  weight   25 - 30 ~were    used.  Mice were divided   into five groups,   that  were:  K- was  control  (aquadest   0)   mL); K+ was  injected  with TCDD  at  dose  of 7 µg/kg   BW;  Pl  was  injected  with TCCD  and  administrated with   Kebar   extract   (0,045 mg/  g  BW/day);   P2 was   injected   with   TCCD  and administrated  with  Kebar  extract  (0,080 mg/ g BW/day);   P3 was  injected  with TCDD and  administrated with  Kebar extract  (0,135 mg/  g BW/day).   Kebar grass extract  was  administrated in 53 days.  Mice were sacrificed  and  right  testis organs were  taken.  Then, histology   preparat  with  HE  staining   were  made  and  Sertoli cells  were  counted.    Data were   analyzed  by  One  Way  ANOV A  followed   by Duncan test  (a  =  0,05).   The result  of this  study   showed that  administration  of Kebar  grass  extract  affected  amount  of mice sertoli  cells.  K+ (2,92a±0,109) showed significance difference  (p<0,05) compared   to Pl (5,00b±0,374), P2 (7,64c±0,409)  and P3 (9,68ct±0,363).  Meanwhile, amount   of Sertoli cells of P3 at the highest  dose  did not  show  significance difference   (p>0,05)  with  K-  (10,16<l±0,829).  The conclusion of this study was  administration of Kebar  grass  extract  per  oral in 53 days  could maintain amount  of mice Sertoli cell exposed  by TCDD. 
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST THE HISTOPATHOLOGICAL CHANGES OF KIDNEY ON MALE RATS (Rattus norvegicus) Cahyono, Shahani Azpriyanne; Eliyani, Hana; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani; Hidajati, Nove; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20415

Abstract

This research was conducted to observe the effect of polypropylene plastic residue on heated palm oil against the histopathological changes on rat's kidney. There were 18 male rats (Rattus norvegicus) that used as the examination animal and were divided into three groups and were given the mixture of heated palm oil and polypropylene plastic per oral with intubation needle. The treatment was done for five weeks. The treatment for P0 group was 84 g of palm oil, for P1 was 84 g of palm oil with 2.3 g of plastic, and for the P2 was 84 g of palm oil with 6.8 g of plastic. The histopathological changes of the kidney were observed using microscope with 400x magnification and the data was analysed using Kruskal-Wallis and continued with Mann Whitney U test. The result showed that there were significant differences between three groups of treatment (P<0.05). There were effects of polypropylene plastic residue on heated palm oil against the histopathological changes of kidney in this research such as cast, epithelial sloughing and necrosis in the mild scale.
Pengaruh Aflatoksin 9,58 ppb dalam Pakan Terhadap Biometrik Saluran Cerna dan Berat Badan Ayam Petelur Periode Awal Pertumbuhan Pratama, Dio Putra; Tehupuring, Benjamin Chr.; Poetranto, Emmanuel Djoko; Eliyani, Hana; Soeharsono, Soeharsono; Tyasningsih, Wiwiek; Hendarti, Gracia Angelina
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28585

Abstract

The purpose of this study was to determine the effect of aflatoxin content of 9.58 ppb in feed on gastrointestinal biometrics and weight gain of laying hens in the early growth period. Thirty-six day old chicks (DOC) were used in this study and were divided into two groups P0 and P1. P0 (control group) was fed with 100% basal diet and P1 (treatment group) was fed a diet with a composition of 80% basal feed + 20% aflatoxin-containing feed. After the DOC was adapted, the first feeding containing aflatoxin was given from the 20th day to the 60th day. Data collection was carried out three times, namely the 20th, 40th, and 60th days. At the end of each period, the chickens would be euthanized to collect data on the weight and length of the digestive tract and body weight. Weight gain with time increased, but had no significant effect (p>0.05) between the control and treatment groups. Body weight continues to increase because the body's homeresis function is still running well. Observations on gastrointestinal biometrics showed significant differences (p<0.05) in oesophagus weight, proventriculus weight, ventriculus weight, jejunum weight, ileum weight and length, and cecum weight because aflatoxins have a direct effect on the digestive tract they pass. The conclusion of this study was that the provision of 20% aflatoxin-containing feed did not affect weight gain but had shown an effect on the biometrics of several gastrointestinal tracts.
Effect of Aflatoxin Contaminated Feed for 40 Days on Anatomy and Histopathology of the Thymus of Laying Hens in the Starter Phase Christy, Rachella; Soeharsono, Soeharsono; Ismudiono, Ismudiono; Eliyani, Hana; Sarmanu, Sarmanu; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28679

Abstract

The purpose of this study was to determine the effect of aflatoxin contaminated feed given for 40 days on the anatomy and histopathology of the thymus of laying hens in the starter phase. A total of 36 DOC laying hens were divided into two groups (P0 and P1). P0 (control group) was given 100% basal starter feed CP 521 and P1 (treatment group) was given 80% basal feed CP 524 plus 20% aflatoxin contaminated feed. Feeding contaminated with aflatoxin was carried out for 40 days, starting on the 20th to the 60th day after the DOC of laying hens was adapted for 20 days. The time of surgery in this study was carried out on the 20th, 40th, and 60th days, where at each surgery time the thymus organ was taken to collect weight data and histopathological preparations were made. The thymus weights based on time showed a significant decrease, but the intergroup thymus weights did not show a significant decrease. The decrease in thymus weight is caused by the involution process. Microscopic observations based on increased cell debris and congestion showed that P0 did not show a significant difference, but P1 showed a significant difference (p>0.05) on the 40th and 60th days. The conclusion in this study was that as much as 20% of feed contaminated with aflatoxins had not affected changes in thymus weight but had shown damage to the histopathological structure of the thymus.
Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4) Anugrah, Alvyan Lantang; Eliyani, Hana; Utomo, Budi; Susilowati, Suherni; Mafruchati, Maslichah; Kurnijasanti, Rochmah
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31812

Abstract

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.
Co-Authors Adikara, Tatang Santanu Anton Sulistiono Anugrah, Alvyan Lantang Benjamin C Tehupuring Berlina, Cyrcilia Relita Budi Utomo Cahyono, Shahani Azpriyanne Chairul Anwar Chrismanto, Dony Christy, Rachella Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri Fadila Zikri Amanda Hani Plumeriastuti Hendarti, Gracia Angelina Hermin Ratnani Hidajati, Nove IMAM MUSTOFA Indah Norma Triana, Indah Norma Ismudiono Ismudiono, Ismudiono Jihan Pangestuningrum Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Machmudia, Ainun Mafruchati, Maslichah Mayola, Dilla Aranda Mufasirin Muhammad Ahdi Kurniawan Mujahid Mujahid, Mujahid Nunuk Dyah Retno Lastuti Poedji Hastutiek Poetranto, Emmanuel Djoko Prastyaningtyas, Kukuh Pratama, Dio Putra Rahmi Sugihartuti, Rahmi Ratna Damayanti Rimayanti Rimayanti Rochmah Kurnijasanti Rochmi, Siti Eliana Sami, Abdul Santoso, Kuncoro Puguh Sarmanu, Sarmanu Soeharsono Soeharsono Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati Suherni Susilowati Suzanita Utama Talita Yuanda Reksa Tehupuring, Benjamin Chr Tehupuring, Benjamin Chr. Tjuk Imam Restiadi Tri Wahyu Suprayogi Tyasningsih, Wiwiek Wahidan Qodiip Maulana Warsito, Sunaryo Hadi Wenny Nur Azizah Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Wurlina, W