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Inneke F. M Rumengan
Fakultas Perikanan Dan Ilmu Kelautan Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Nursing Public Health Other

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A REVIEW OF ANTIFOULING BIOCIDES CONTAMINATIONS IN INDONESIA, MALAYSIA, THAILAND AND VIETNAM Harino, Hiroya; Yatsuzuka, Emi; Arifin, Zainal; Rumengan, Inneke F. M.; Ismail, Ahmad; Wattayakorn, Gullaya; Inoue, Koji
Marine Research in Indonesia Vol 38 No 2 (2013)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1702.54 KB) | DOI: 10.14203/mri.v38i2.61

Abstract

The concentrations and the distribution of organotin (OT) compounds and booster biocides in sediment and biological samples from Indonesia, Malaysia, Thailand and Vietnam are reviewed. Basically, the concentrations of tributyltin (TBT) in these countries were within the levels that have been reported in developed countries. However, the concentrations of triphenyltin (TPT) and booster biocides were lower than the reported values. Concerning the spatial distribution of antifouling biocides, higher concentrations of OTs were detected in the industrial area and fishing ports. Among ASEAN countries, the highest concentrations of TBT and TPT were observed in Bitung, Indonesia, where many ships from Southeast Asia visit and is moored in the port. The ratio of detected alternative biocide differed from country to country. Especially, many booster biocides were detected and their concentrations were high in Vietnam.
DISTRIBUTION OF MONOGONONT ROTIFERS, Branchionus spp IN NORTH SULAWESI Rimper, Joice; Kaswadji, Richardus; Widigdo, Bambang; Sugiri, Nawangsari; Rumengan, Inneke
Marine Research in Indonesia Vol 32 No 2 (2007)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7372.399 KB) | DOI: 10.14203/mri.v32i2.443

Abstract

In order to characterize the distribution pattern of rotifers Brachionus spp. in North Sulawesi, sample collections have been conducted at four locations, two at east coast and other two at west coast of North Sulawesi peninsula, which are connected to Maluku and Sulawesi Seas, respectively. At each location, a 40 p.m mesh size plankton net with a 0.3 m opening diameter was horizontally towed at three sites to represent coastal water, estuary and brachkiswater pond. Sampling periods were in January and August 2005 to represent west monsoon and east monsoon, respectively. Each sampling was performed twice a day during high and low tide. Some environmental factors were also measured in situ during the sampling. Three species of Brachionus were found during the survey, Brachionus rotundiformis, B. caudatus and B. quadridentatus. Their abundances were significantly different, both between locations and sampling sites. However, among the similar habitat such as between Manembo-nembo and Minanga brachkishwater ponds, B. rotundiforms abundance was not significantly different. This species was the most abundant compared to the other two species, in average 1984,4 ind/m3, while the other species was as low as 26,1 ind/m3 and 212,9 ind/m3 for B. caudatus and B. quadridentatus, respectively. The abundance of rotifers in northern areas connected to Molluccas Sea is higher compared to those in the southern areas connected to Sulawesi Sea. Distribution of B. caudatus is very similar to those of B. rotundiformis, while B. quadridentatus appears to have its specific pattern. Results of this study suggest that the abundance and species composition of the rotifers reflect trophic status of the aquatic ecosystem in the area. It is still awaiting future studies to elaborate the impacts of the trophic gradients in horizontal distribution of rotifers. Therefore, it is necessary to conduct a long term monitoring with emphasis on the structure of rotifer assemblages in a variety of sites subjected to environmental perturbations, including anthropogenic contaminant inputs.
The effectivity of polysaccharide extracted from marine algae, Euchema cottonii, on the immune response of tilapia, Oreochromis niloticus ., Indriasari; Mangindaan, Remy E.P.; Rumengan, Inneke F.M.
AQUATIC SCIENCE & MANAGEMENT Vol 2, No 1 (2014): April
Publisher : AQUATIC SCIENCE & MANAGEMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.2.1.2014.12387

Abstract

Title (Bahasa Indonesia): Efektifitas polisakarida yang diekstrak dari alga, Euchema cottonii, terhadap respon imun ikan nila (Oreochromis niloticus)This study was aimed to test the effectiveness of polysaccharide extracts of algae, Eucheuma cottonii, in increasing the nonspecific immune response and to get an extract with best efficacy on the immune response of the tilapia, Oreochromis niloticus. The design used completely randomized design (CRD) using 4 treatments, 0 mg/kg of saline material (A), 10 mg/kg of E. cottonii extract (B), 10 mg/kg of Iota (C), and 100 cells/ml of formaline killed vaccine (FKV), Aeromonas hydrophilla, each of which were with 3 (three) replications. The fish were acclimated for 2 weeks, and then treated for 4 weeks. Test animals were vaccinated through intraperitoneal injection 2 times at one week interval as much as 0.2 ml FKV taken with disposable plastic 1 ml syringe. The vaccination was done injecting the bacteria, A. hydrophilla, previously killed in 0.4% formaldehyde FKV at the density of 109 cells / ml. Then the test fish were injected with the test material extract solution. E. cottonii as much as 0.2 ml / fish. Injection point was between the pelvic fins and the lateral line near the anus. The results showed that administration of the polysaccharide extracted from algae in tilapia gave significant effect on nonspecific immune response increment of the fish (total leukocytes and phagocytic activity). Penelitian ini bertujuan untuk menguji keefektifan ekstrak polysakarida alga laut, Eucheuma cottonii, dalam meningkatkan respons kebal non-spesifik dan untuk mendapatkan suatu ekstrak dengan kualitas terbaik terhadap respons kebal ikan nila, Oreochromis niloticus.  Penelitian ini mengguna-kan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan yang digunakan adalah A = 0 mg.kg-1 untuk bahan uji saline; B = 10 mg.kg-1 ekstrak alga E. cottonii; C = 10 mg.kg-1 untuk iota; D = FKV A. hydrophilla 109 sel/ml. Ikan nila pertama-tama dipelihara dalam kolam (2 x 1 m) selama 2 minggu untuk proses aklimatisasi dan kemudian diberi perlakuan selama 4 minggu. Hewan uji divaksinasi dengan injeksi secara intraperitoneal (IP) dilakukan 2 kali dengan selang waktu seminggu sebanyak 0,2 ml FKV yang diambil dengan plastic syringe disposable 1 ml. Vaksinasi dilakukan dengan menginjeksikan bakteri A. hydrophilla yang telah dimatikan dengan formalin 0,4% FKV pada kepadatan 109 sel/ml. Kemudian ikan uji disuntik dengan bahan uji larutan ekstrak E. cottonii dengan dosis penyuntikan 0,2 ml larutan/ikan. Titik suntik diantara sirip perut dan lateral line dekat anus.Hasil penelitian menunjukkan pemberian polisakarida yang diekstrak dari alga pada ikan nila memberi pengaruh yang nyata terhadap peningkatan respon imun nonspesifik ikan (total leukosit dan aktivitas fagositosis).
Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida) Letsoin, Petrus P; Pangkey, Henneke; Sampekalo, Julius; Rumengan, Inneke F.M; Wullur, Stenly; Rimper, Joice R.S.T.L
AQUATIC SCIENCE & MANAGEMENT Vol 1, No 2 (2013): Oktober
Publisher : Graduate Program of Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.1.2.2013.7276

Abstract

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae.  Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.
ACUTE AND CHRONIC EFFECT OF CYANIADE ON SURVIVAL RATE, BEHAVIOR, AND REPRODUCTION OF DAPHNIA SP. Pattiwael, Melky R; Mangindaan, Remy E. P; Prabowo, Rudi; Rumengan, Inneke F. M
AQUATIC SCIENCE & MANAGEMENT Vol 1, No 1 (2013): April
Publisher : Graduate Program of Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.1.1.2013.1969

Abstract

Cyanide (CN) through leaching process in the gold processing could produce tailings that have negative impacts on the aquatic environment. To determine the acute and chronic effects of cyanide on aquatic organisms, toxicity tests have been conducted in the laboratory using Daphnia sp.This zooplankton organism is recommended by APHA as standard test animals. In this study the test concentrations refer to the Ministerial Decree No. 202 of 2004 and Government Regulation No. 82 of 2001. Cyanide was analyzed as free CN and WADS CN. The result of acute toxicity test using cyanide solution showed that Daphnia could survive a maximum concentration of 0.2 ppm. LC50 values for 24 and 72 hours were 0,59 mg/L and 0,10 mg/l, respectively. The LT50 was found at 42 hours. Daphnia sp. produced different numbers of offspring at different CN concentrations, and changed their reproduction pattern from parthenogenesis to sexual reproduction after exposure to a cyanide concentration of 0.1 ppm for 24 hours©  Sianida (CN) melalui proses leaching dalam proses pengolahan emas menghasilkan limbah yang dapat memberi dampak negatif bagi lingkungan perairan. Untuk mengetahui sampai seberapa jauh CN dapat memberi efek akut dan kronis terhadap biota air, telah dilakukan uji toksisitas di laboratorium dengan menggunakan Daphnia sp. Zooplankton ini direkomendasikan oleh APHA sebagai salah satu hewan uji standar. Dalam penelitian ini konsentrasi uji yang dipakai mengacu pada peraturan yang berlaku, yaitu Keputusan Menteri No. 202, Tahun 2004, dan Peraturan Pemerintah No. 82, Tahun 2001. Analisis sianida yang dihitung adalah nilai free CN dan WADS CN. Hasil penelitian uji toksisitas akut dengan menggunakan larutan sianida didapati Daphnia mampu bertahan hidup sampai pada konsentrasi 0,2 ppm dan nilai LC50 berada pada konsentarsi 0,1 ppm serta LT50 pada jam ke 42. Hasil uji efek kronis, Daphnia sp. menghasilkan jumlah anakan yang berbeda pada konsentrasi CN yang berlainan, dan mengalami perubahan pola reproduksi dari partenogenesis menjadi seksual setelah dipaparkan pada kosentrasi sianida 0,1 ppm selama 24 jam©
The effects of stimulant growth hormones on tissue culture of seaweed Kappaphycus alvarezii in vitro Fadel, Ariyati H; Gerung, Grevo S; Suryati, Emma; Rumengan, Inneke F.M
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 1 (2013): Mei
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2013.2282

Abstract

In order to anticipate the qualified and sustainable seed requirement for seaweed culture, it is necessary to conduct tissue culture for vegetative cultivation of isolated leaves, bud, and stemin an artificial medium enriched with nutrient and growth regulator. The purpose of this study is to obtain newly grown plant in a big quantity in relatively short period of time, with physiological and morphological properties similar to the stocks. Culture media used were Grund Medium and PES with an addition of a growth regulator, IAA (Indol acetic acid) and BAP (Benzil amino purin). The buds produced were buds with similar properties as the parent. The longest bud (1,851 mm) was obtained in Grund Medium with IAA treatment, while the length of bud in PES medium was only 0.612 mm. The number of buds was highest (10,6)  in Grund media with IAA+BAP (1:1) treatment, and 6,82 with IAA treatment in PES media. The survival rate of explants was highest in media enriched with 0.5 mg/L IAA (indol acetic acid). The best media for growing seaweed Kappaphycus alvarezii was Grund Medium© Untuk mengantisipasi kebutuhan bibit yang berkualitas dan tersedia  secara kontinyu, diperlukan suatu upaya kultur jaringan untuk perbanyakan tanaman secara vegetatif dengan mengisolasi bagian tanaman seperti daun, mata tunas, serta batang dalam media buatan secara aseptik yang diperkaya dengan nutrien dan zat perangsang tumbuh. Tujuannya untuk mendapatkan tanaman baru dalam jumlah banyak dalam waktu yang relatif singkat, yang mempunyai sifat fisiologi dan morfologis sama dengan tanaman induknya. Media kultur yang digunakan adalah media Grund Medium dan PES dengan penambahan zat perangsang tumbuh yaitu IAA (Indol acetic acid) dan BAP (Benzil amino purin). Tunas yang dihasilkan merupakan anakan yang mempunyai sifat yang sama dengan induknya.  Panjang tunas tertinggi dicapai pada media Grund Medium dengan perlakuan IAA (1,851 mm) dan media PES sebesar 0,612 mm. Sedangkan jumlah tunas tertinggi dicapai perlakuan IAA+BAP (1:1) sebesar 10,6 pada media Grund dan perlakuan IAA sebesar 6,82 pada media PES. Untuk tingkat kelangsungan hidup (sintasan) eksplan yang paling baik pada media yang diberikan pupuk IAA (indol acetic acit) dengan kosentrasi 0,5 mg/L. sedangkan media yang baik untuk pertumbuhan rumput laut Kappaphycus alvarezii adalah media Grund Medium©
The yield, nitrogen content, and dye’s binding capacity of chitin and chitosan of rotifer Brachionus rotundiformis Modaso, Riny; Suryanto, Edi; Tallei, Trina; Rumengan, Inneke F.M
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 1 (2013): Mei
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2013.2286

Abstract

Chitin and chitosan from rotifer have not been previously explored due to the problem of high biomass required for extraction. This study aimed to obtain the chitin yield from rotifer biomass produced in mass culture, and to characterize the basic properties of chitin and chitosan, especially, nitrogen content and dye binding capacity. Methods of extraction and deacetylation of chitin were adopted from Chandumpai et al. (2004) with modification. The nitrogen content was analyzed using the semi-micro Kjeldahl method (AOAC, 1984), and dye binding capacity using the method developed by Cho et al. (1998). Results show that the yield of chitin obtained from the rotifer sample was relatively small (4.64%), and the yield of chitosan was even smaller, only 2,62%. The proportion of chitosan over chitin was 52,4%. The nitrogen content of chitin and chitosan of rotifer were 4.23 to 4.36% and 7.12-7.23%, respectively. The capacity of chitin to maintain the bonded dye was relatively stronger than that of chitosan, but the chitosan had higher capacity to absorb the dye. The characterization of other important properties of chitin and chitosan to be developed as biopolymer for industry is further aspects to be assessed© Eksplorasi kitin dan kitosan dari rotifer belum pernah dilakukan sebelumnya karena dihadapkan pada permasalahan kebutuhkan biomassa yang banyak. Penelitian ini bertujuan untuk memperoleh rendemen kitin dari biomassa rotifer hasil kultur massal, dan menjajaki karakterisasi awal sifat fisika kimia kitin yang mendasar yaitu daya ikat zat warna dan kadar nitrogen. Ekstraksi kitin dan kitosan dilakukan berdasarkan metode Chandupai et al. (2004), kadar nitrogen menggunakan metode semi-micro Kjeldahl (AOAC, 1984), dan daya ikat zar warna ditentukan menurut metode Cho et al. (1998). Dari penelitian ini, rendemen kitin yang diperoleh dari hasil ekstraksi relatif kecil berkisar 4,64 %, dan rendemen kitosan bahkan lebih kecil lagi hanya 2,62%. Proporsi kitosan dari kitin yaitu 52.4 %. Kadar nitrogen pada kitin rotifer berkisar 4,23-4,36 % dan kitosan berkisar dari 7,12-7,23%. Kitin memiliki sifat yang lebih baik dalam ketahanan untuk mengikat zat warna sebaliknya kitosan memperlihatkan sifatnya yang dapat mengikat zat warna dalam tingkat adsorpsi yang tinggi dibanding kitin. Karakterisasi  sifat fisika kimia kitin dan kitosan yang penting lainnya untuk pengembangannya sebagai biopolimer dalam skala industri, merupakan aspek-aspek yang perlu dikaji lebih lanjut©
Identifikasi Mikroba yang Koeksis Dengan Ascidia Lissoclinum patella Menggunakan Sekuens Gen 16S rRNA Untu, Patricia; Rumengan, Inneke F. M.; Ginting, Elvy L.
JURNAL PESISIR DAN LAUT TROPIS Vol 3, No 2 (2015): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.3.2.2015.10110

Abstract

Penelitian ini dilakukan dengan tujuan untuk menentukan jenis mikroba koeksis denganascidia Lissoclinum patella menggunakan sekuens gen 16S rRNA. Sampel yang digunakandalam penelitian ini diambil dari jaringan tissue pada ascidia L. patella yang diambil dariperairan Malalayang, Sulawesi Utara. Sampel mikroba diinokulasi dalam media Hirata dandikultur selama ± 1 minggu. Sampel mikroba tersebut diisolasi DNA, amplifikasi melalui PCR(Polymerase Chain Reaction), elektroforesis gel agarose dan dianalisis data DNAnyamenggunakan BLAST pada NCBI (National Center for Biotechnology Information). Identifikasiyang dilakukan menggunakan BLAST diperoleh hasil 15 mikroba yang memiliki tingkatkemiripan yang tinggi dengan sekuens gen 16S rRNA sampel mikroba yaitu cyanobacteriumenrichment culture CAWBG121 dan CAWBG120 clone, uncultured Symploca sp. clone DRTO-55, Leptolyngbya sp. PCC7376 complete genome, Leptolyngbya sp. PCC7376, unculturedbacterium clone PINFEBB02, uncultured bacterium clone 5M47, Synechococcus elongatusCCMP1630, uncultured bacterium clone reef H09, Synechococcus sp. PCC7002 completegenome, Synechococcus sp. 16S rRNA gene strain PCC7002, Synechococcus sp. DNA untuk16 ribosomal RNA, Synechoccous sp. L21-BG-1, Oscillatoria rosea IAM M-220 danSynechococcus sp. PCC 8807. Tingkat kemiripan mikroba dalam NCBI dengan sampel H1berkisar antara 98-99 %.
karakteristik pertumbuhan populasi rotifer (Brachionus rotundiformis) tanpa pemberian aerasi dan mikroalga sebagai pakan pada media kadar garam berbeda Fembri, Fransiskus; Kaligis, Erly; Rumengan, Inneke
JURNAL PESISIR DAN LAUT TROPIS Vol 5, No 1 (2017): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.5.1.2017.14996

Abstract

Rotifer sangat populer sebagai biokapsul bagi larva fauna laut, karena menjadi pentransfer nutrien, mikromolekul, asam amino dan asam lemak tak jenuh tingkat tinggi, mineral, vitamin dan antibiotik dari lingkungan hidup ke larva tanpa efek polutan. Metode penelitian yang dilakukan adalah percobaan kultur rotifer dalam kondisi laboratorium  dengan menggunakan media kadar garam berbeda (20, 25, 30 dan 35 ppt). Penelitian ini bertujuan untuk mengetahui laju pertumbuhan populasi dan proporsi betina rotifer yang membawa telur pada setiap perlakuan. Hasil penelitian menunjukan laju pertumbuhan populasi tertinggi pada kadar garam 20 ppt menghasilkan peningkatan pertumbuhan populasi tertinggi pada hari ke-3 yaitu 0,66 sedangkan pada kadar garam lainnya yaitu 25 ppt, 30 ppt, dan 35 ppt nilai r yang diperoleh adalah 0,45 ; 0,26 ; 0,22.  Berdasarkan proporsi betina yang membawa telur hasil tertinggi dicapai pada perlakuan kadar garam 20 ppt yaitu 56.7% pada hari pertama, kemudian pada hari selanjutnya terjadi penurunan. Pada perlakuan kadar garam 20 ppt penurunan terjadi karena pertumbuhan populasi yang tinggi tidak disertai dengan jumlah individu yang membawa telur, sehingga pertumbuhan pada kadar garam 35 ppt lebih tinggi mulai pada hari ke-2 hingga hari ke-5.
PRODUKSI DAN FRAKSINASI ASAP CAIR DARI LIMBAH TONGKOL JAGUNG UNTUK PENGHAMBATAN PEROKSIDASI LIPIDA IKAN LAYANG (Decapterus russelli) Mongan, Jemsi; Suryanto, Edi; Rumengan, Inneke
CHEMISTRY PROGRESS Vol 4, No 1 (2011)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.4.1.2011.26503

Abstract

ABSTRACTMongan et al., 2011. Production and fractionation of liquid smoke from corn cob to inhibit lipid peroxidation oflaying fish (Decapterus russelli).Corncob is a cellulose material and can be made to liquid smoke by pyrolysis. Liquid smoke from pyrolysis canbe purified using adsorbent. Chitin is an adsorbent produced by crustacea shell. Liquid smoke purified by chitincan act as antimicroba and antioxidant. Layang fish is very abundantly in Gorontalo Province, so preservativetechniques were needed to prevent lipids peroxidation in fish. The aims of this research was to determineantioxidative activity of liquid smoke from corncop to prevent lipids peroxidation on layang fish.This research using laboratory experiment methods to analyze total phenolic content, free radical scavengingactivity, total antioxidant and lipids peroxidation on layang fish by spectrophotometer UV-vis. This researchusing complete random design (rancangan acak lengkap, RAL), thus obtained data were analyzed usingstatistic. Significant difference between groups were analyzed using ANOVA with p<0,05. If there is significantdifference between groups, data were analyzed using Duncan’s multiple range test (DMRT).The results showed that liquid smoke can be produced using corncop by pyrolysis is 31,7 %. Fractioned liquidsmoke using chitin obtained 4 fractions were fraction I, II, III and IV. Liquid smoke and fractioned liquid smokecontain total phenolic content are 280, 273, 280, 286 and 301 mg/kg gallic acid, respectively. Free radicalscavenging activity from liquid smoke and fractioned liquid smoke are 87,39, 86,08, 89,29, 89,90 and 93,73%,respectively. Although, total antioxidant from liquid smoke and fractioned liquid smoke are 59, 35, 51,32, 51,57,52,74 and 53,58 ppm, respectively. Fraction IV of liquid smoke fractioned with chitin was used in antioxidativeactivity of laying fish test. Fraction IV with concentration 25% can inhibit lipid peroxidation on fresh layang fishby 19 mikromolar for two days. Although layang fish without liquid smoke shows high lipid peroxidation by 24,96mikromolar. Fraction IV with concentration 25% can inhibit lipid peroxidation on layang fish by 18,28 mikromolarfor two days. Antioxidative activity from liquid smoke caused by it phenolics compound that act as antioxidant.As conclusions of this research, liquid smoke can be produced from corncob and fractioned with chitin. Liquidsmoke contain phenolics and act as antioxidant. Liquid smoke can inhibit lipids peroxidation in layang fish. Thisresearch suggested that liquid smoke can be used to inhibit lipid peroxidation on layang fish..Keywords : liquid smoke, corn cob, peroxidation, layang fish
Co-Authors Ahmad Ismail Akerina, J. Alfret Luasunaung Anggraeny, Dyta Anindito Leksono Ariyati H Fadel Budiyanto Budiyanto Calvyn F. A. Sondak, Calvyn F. A. Carolus Paulus Paruntu D. Sumilat Daniel Limbong Deiske Adeliene Sumilat, Deiske Adeliene Didit Dewanto Edi Suryanto Elvy L. Ginting, Elvy L. Emma Suryati Erly Kaligis Erly Kaligis F. Losung, F. Fallen B. Sandana Fembri, Fransiskus Gerung, Pramulya R.A Ginting, Elvy Like Gregoria S. S. Djarkasi Grevo S Gerung Harino, Hiroya Harino, Hiroya Henneke Pangkey Hens Onibala Herson, Nur Afiah Hety B Lahope Indra R.N. Salindeho Indriasari ., Indriasari Inoue, Koji Inoue, Koji Ixchel F Mandagi J. Rimper Jantje Pongoh John Kekenusa Johny Budiman Joice R.T.S.L Rimper Joppy Mudeng Julius Sampekalo K. W.A. Masengi Lahimade, Melisa Lintang, Rosita AJ Lucia Cecilia Mandey Lumuindong, Frans Luntungan, Aldian H. Luntungan, Aldian H. Makapedua, Daisy M. Manoppo, Victoria Manu, Lusia Markus T. Lasut Marseni Sulung Masengi, Akira W. R. Masengi, E. I. K.G. Masengi, K.W.A Melky R Pattiwael Mongan, Jemsi Mopay, Maratade N. D. Rumampuk, N. D. N.D. Rumampuk Nasution, Ali Napiah Nawangsari Sugiri Pangemaman, Trezya Nilam Sari Pangkey, Henneke D. Patricia Untu, Patricia Petrus P Letsoin Pipih Suptijah Rampengan, M. M.F. Rampengan, Royke Remy E. P Mangindaan RICHARDUS KASWADJI Rignolda Djamaluddin Rina Kundre Rinny Modaso Riny Modaso Rompas, Gebriela Roring, Jordan Iglesias Rudi Prabowo Rumampuk, N.D. Salindeho, Netty Salindeho, Netty Sammy N.J. Longdong Songgigilan, Anisha M.G Sri Yuningsih Noor Stenly Wullur Sumampouw, Eliza J Sumilat, Deiske A. A. Sumual, Maria Fransisca Talumepa, Anggun C. N. Trina Tallei Veibe Warouw Wattayakorn, Gullaya Wattayakorn, Gullaya Wilhelmina Patty Wulur, Stenly Yatsuzuka, Emi Yatsuzuka, Emi Zammrud Lantiunga Zebua, Nistiarni