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All Journal Jurnal Kedokteran Brawijaya Alchemy : Journal of Chemistry Jurnal Ilmu Kimia Universitas Brawijaya Indonesian Green Technology Journal Natural B The Journal of Pure and Applied Chemistry Research EduChemia: Jurnal Kimia dan Pendidikan Science and Technology Indonesia ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Jurnal Fish Protech JKPK (Jurnal Kimia dan Pendidikan Kimia) Rengganis Jurnal Pengabdian Masyarakat Jurnal Sains dan Teknologi Makara Journal of Science International Journal of Renewable Energy Development al Kimiya : Jurnal Ilmu Kimia dan Terapan
Akhmad Sabarudin
Laboratory of Analytical Chemistry, Chemistry Department, Brawijaya University
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Other

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Non-Enzymatic Detection of Glucose and Ketones in Urine using Paper-Based Analytical Devices 'Aisy, Kamila Rohadatul; Fahmi, Ahmad Luthfi; Sulistyarti, Hermin; Wulandari, Ika Oktavia; Sabarudin, Akhmad
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 9, No 2 (2024): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v9i2.87294

Abstract

Diabetes, driven by unbalanced diets and unhealthy lifestyles, is highly prevalent. In Indonesia, its prevalence is projected to reach 28.6 million by 2045. Microfluidic paper-based analytical devices (μPADs) are paper-based analytical tools that use hydrophilic paper for measurement and hydrophobic barriers to control fluid flow. This research aims to develop a non-enzymatic method for detecting glucose and ketones in artificial urine using S2Z-μPADs. The fabrication of S2Z-μPADs involves printing the design on Whatman No. 1 paper using wax printing and applying silver nanoparticles for glucose detection and the Schiff base reaction for ketone detection. The results show that the optimum condition for glucose detection is achieved with an AgNO3 concentration of 500 mM. A NaOH concentration of 10 M. Acetoacetate detection is optimized with a glycine concentration of 1 M, sodium nitroprusside concentration of 15%, NaOH concentration of 1 M, a drying time of 8 minutes, and a reaction time of 10 minutes. Validation results demonstrate good linearity for glucose (R² = 0.9821) and ketones (R² = 0.995). High precision was achieved with relative standard deviation (RSD) values of 3.792% for glucose and 1.482% for ketones. The obtained limits of detection (LOD) and limits of quantification (LOQ) indicate that the developed S2Z-μPADs can differentiate between each category of diabetes. The accuracy of glucose and ketone detection ranges from 87.463% to 97.374%. The high accuracy of the μPADs highlights their potential for reliable diabetes management and effective disease monitoring.
Co-Authors 'Aisy, Kamila Rohadatul Abdan, Ahmad Multazam Ani Mulyasuryani Arie Srihardyastutie Athiyah, Izzatul Audina Hardiyanti, Shova Aulanni’am Baiq Octaviana Dwi Anggraeny Barlah Rumhayati Darjito Darjito Diah Mardiana Edi Widjajanto Eka Ratri Noor Wulandari Elok Kamilah Hayati Elvina Dhiaul Iftitah Erwanto Erwin Sulistyo Fadhila, Salma Iza Fahmi, Ahmad Luthfi Fiddaroini, Saidun Hani Susanti, Hani Haresmawati, Unsania Haryanto, Aura Ananda Hermin Sulistyarti Hidayaty, Alfianita Nuril Hikmanita Lisan Nashukha Ibrahim, Moh. Nuh Iftitah, Elvina D. Ika Oktavia Wulandari Ikhsan, Aqshal Nur Kasanah, Izza Lailatul Khabibi, Bhisma Wildan Khanifah, Nurul Layta Dinira Layta Dinira Lukman Hakim M. Rafi Madaniyah, Luailik Moh. Farid Rahman Mohamad Nur Ibrahim Mohamed Abousa Gaza Mufidah Sari, Puspita Nalle, Ferry Chrismiadi Nugraha, Hedhi Setya Nur Permatasari Nur Samsu Okky Anggraito Pratikno, Mochammad Yosi Putra, Boyfannie Ivan Putri, Krista Firdaus Suwarno Putri, Lani Artana Qonitah Fardiyah Raeni, Septi F. Raeni, Septi Fajar Rafif, Sullahudin Ahmad Rafika, Nilna Assasiatur Rahayu, Lale B Rahayu, Lale Budi Hutami Riva’i, Imam Roja’i, Abdul Munir Rurini Retnowati Salsabila, Isadora Evani Santjojo, Djoko Harry Setyawan Purnomo Sakti Silvi Avianti Indriana Suci Amalia Ulayya, Salwa Utama, Muhammad Mashuri Vita Tria Mardila Warsito Warsito Yudha Ikoma Istanti