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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia VALENSI ILMU KELAUTAN: Indonesian Journal of Marine Sciences JURNAL KIMIA SAINS DAN APLIKASI Current Biochemistry Indonesian Journal of Human Nutrition Journal of Mathematical and Fundamental Sciences Indonesian Journal of Pharmaceutical Science and Technology Jurnal Sumberdaya Hayati Warta IHP (Warta Industri Hasil Pertanian) ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Chemistry Indonesian Journal of Chemical Research QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Farmamedika (Pharmamedica Journal) Tunas Medika Jurnal Kedokteran & Kesehatan Indonesian Journal of Applied Research (IJAR) Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Journal of Food Technology and Health Journal of Fisheries & Marine Halal Studies and Society
Mega Safithri
Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor Jl. Raya Dramaga Kampus IPB Dramaga Bogor, Jawa Barat 16680 Indonesia
Religion Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Journal : Current Biochemistry

 1   2 
Antibacterial activity of Stichopus hermanii and Stichopus variegatus methanol extract Susanto, Hendra; Safithri, Mega; Tarman, Kustiariyah
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

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Abstract

Since 500 years ago sea cucumber has been used as wound healing medicine. Sea cucumbers are thought to function as a cell growth factor and have the potential as an antibacterial agent. This study aims to determine whether the methanol extract of sea cucumber has antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa so that it can be used to help diabetic wound healing of people infected with the same bacteria. The extraction method used was maceration with methanol to dried frozen simplicia. The simplicia was obtained by freeze drying to liquid preparation of heated fresh sea cucumber. The antibacterial activity test was performed using well diffusion method. There were no any antibacterial activity found in the two methanol extracts of sea cucumber. Keywords: antibacterial agent, Stichopus hermanii, Stichopus variegatus
Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Dwitasari, Oczhinvia; Hami Seno, Djarot Sasongko; Safithri, Mega
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

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Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment
Antimicrobial activity and identification of bioactive compounds of Söfö-söfö (Acmella cf) leaf extract using GC-MS Maria bintang; Faoziduhu Bawamenewi; Mega Safithri; Fachriyan H Pasaribu
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Söfö-söfö is a traditional medicinal plant from Nias Island that can cure fever, cough, diarrhea and fungal infections on the skin. However, the scientific basis of these plants is unknown. The aim of this research was to extract the Söfö-söfö leaf by maceration method using two solvents that were 70 % ethanol and ethyl acetate, to test the antimicrobial activity of the extract by Agar diffusion method against Staphylococcus aureus, Eschercia coli, and Candida albican, and analyze secondary metabolite compounds by fitochemistry test and to determine the components of bioactive compounds by GC-MS. The results showed that the best solvent for making Söfö-söfö extract as antimicrobial is ethyl acetate with a minimum inhibitory concentration of 4000 ppm to S. aureus (1.25 ± 0.35 mm), E. coli (1 mm) and C. albican 6000 ppm (1.5 mm). The secondary metabolite compounds of ethyl acetate extract were alkaloids, flavonoids and steroids. Bioactive compounds found in the Söfö-söfö ethyl acetate extract with potential antimicrobial activity were hexadecanoic acid, stigmasterol, neophytadiene, methyl ester, squalene and phytol.
Antihyperglicemic Activity of Curcuma Xanthorrizha Roxb. Nanocurcuminoid Emulsion on Streptozotocin Induced Sprague-Dawley Rat Irma Rahmayani; Laksmi Ambarsari; Mega Safithri
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Curcuminoid, a natural compound isolated from Curcuma xanthorrhiza RoxB (Temulawak) has been reported to possess antioxidant, anti-inflammatory, anticancer, antimicrobial and anti-hyperglicemic properties. The curcuminoid is weakly soluble in water that restricts its bioavailability. This problem could be overcome by incorporating curcuminoid into solid lipid nanoparticles (SLN). Nanocurcuminoid was prepare by using homogenization-ultra sonication methods. Nanocurcuminoid obtained in this study was 523.5 nm in size and polydispersity index of 0.218 with entrapment efficiency of 24.2 %. Rats were made diabetic by induction of 50 mg / kg BW STZ, treated with the chosen formula (doses 5, 10, 20 mg/kg BW) orally for 15 days. Body weight and blood glucose levels were measured from day 0, 4th,7th,11th and 15th. The results showed that 15 days of daily treatment of 10 mg/kg BW nanocurcuminoid emulsion led to a reduction of blood glucose level by 30.93±14.90 % and body weight by 15.5±13.92 %. Curcuminoid formulated in solid lipid nanoparticles could suppress a decrease in body weight and lower blood glucose levels of rats.
Antibacterial Activities of Sirih Merah (Piper crocatum) Leaf Extracts Puspa Julistia Puspita; Mega Safithri; Nirmala Peni Sugiharti
Current Biochemistry Vol. 5 No. 3 (2018)
Publisher : IPB University

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Abstract

Piper crocatum is one of medicinal herbal plants with a large number of benefits. Usually herbal plants have activity as antibacterial agent. Therefore, the objectives of this research were to obtain information on antibacterial activities of the leaf extracts of Piper crocatum againts four types of bacteria, in that Staphylococcus, Bacillus substilis, Escherichia coli, and Pseudomonas aeruginosa and then to analyze the phytochemistry of the leaf extracts of Piper crocatum. The leaves of Piper crocatum were extracted by maceration and reflux using ethanol 30%. The assays of the antibacterial activities and phytochemistry on the extracts were carried out using the method of Maria Bintang. Results showed that the yield of the extraction using ethanol by maceration method was 20.8%. Meanwhile, using the reflux method, the yield was obtained about 26.25%. The phytochemistry analysis showed that the leaf extracts of Piper crocatum contained alkaloid, steroid and tanin. According to this study, it was found that the leaf extract of Piper crocatum can be used to inhibit the growth of B. subtilis and P. aeuruginosa, but can not inhibit the growth of E.coli and S. aureus.
Toxicity Extract and Faction of Surian Toona sinensis Leaf and Bark against Shrimp Larvae Artemia salina L. Rori Theresia; Syamsul Falah; Mega Safithri; Muhammad Assyar
Current Biochemistry Vol. 3 No. 3 (2016)
Publisher : IPB University

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Abstract

Surian (Toona sinensis) is one of the herbs that contain many bioactive compounds. Part of surian plants that can be utilized as therapy is leaf and bark. This study aims to determine the toxicity of leaf and bark extract of Surian through Bhrine Shrimp Lethality Test (BSLT), then compare toxic concentration of water extract, and ethanol 70 %, and n-hexane, diethyl ether and ethyl acetate fractions to shrimp larvae (Artemia salina L). The results showed that water content of leaves simplicia was 8.08 % and bark was 6.26 %. The highest extract rendement value by ethanol solvent was 23 %. The result of BSLT test probit analysis showed that the extract of ethyl acetate bark fraction had the highest toxicity with LC50 value 405.641 ppm, while leaf water extract had the lowest toxicity with LC50 value 707.787 ppm.
Antibacterial activity of Stichopus hermanii and Stichopus variegatus methanol extract Hendra Susanto; Mega Safithri; Kustiariyah Tarman
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

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Abstract

Since 500 years ago sea cucumber has been used as wound healing medicine. Sea cucumbers are thought to function as a cell growth factor and have the potential as an antibacterial agent. This study aims to determine whether the methanol extract of sea cucumber has antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa so that it can be used to help diabetic wound healing of people infected with the same bacteria. The extraction method used was maceration with methanol to dried frozen simplicia. The simplicia was obtained by freeze drying to liquid preparation of heated fresh sea cucumber. The antibacterial activity test was performed using well diffusion method. There were no any antibacterial activity found in the two methanol extracts of sea cucumber. Keywords: antibacterial agent, Stichopus hermanii, Stichopus variegatus
Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Oczhinvia Dwitasari; Djarot Sasongko Hami Seno; Mega Safithri
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment
Antibacterial Activity of Ethanol Extract of Spirulina platensis Prayoga Pannindrya; Mega Safithri; Kustiariyah Tarman
Current Biochemistry Vol. 7 No. 2 (2020)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.7.1.1

Abstract

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.
Antibacterial Activity of Ethanol Extract of Red Betel Leaves (Piper crocatum) and Its Fractions against Escherichia coli pBR322 Fernanda Chairunisa; Mega Safithri; Maria Bintang
Current Biochemistry Vol. 9 No. 1 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.1.1

Abstract

Red betel leaf (Piper crocatum) is one of the typical Indonesian herbal plants that has an antibacterial function. This study aimed to test and measure the activity of 70% ethanol extract of red betel leaf and its fractions (n-hexane, ethyl acetate, and water fractions) as antibacterial against specific bacteria Escherichia coli pBR322 based on activity and minimum inhibitory concentration. Extraction was carried out using the maceration method and continued with fractionation based on the level of solvent polarity. Antibacterial activity was indicated by the presence of inhibition zones. The minimum inhibitory concentration was indicated by the appearance of the inhibition zone at the minimum concentration. Results showed that extraction process resulted in crude extract yield of 19.49%. The best antibacterial activity was shown by 1000 ppm n-hexane fraction with an inhibition zone of 2.40 mm ± 0.14. The best minimum inhibitory concentration was 100 ppm n-hexane fraction with an inhibition zone of 0.60 mm ± 0.56.
Co-Authors . SURYANI Abdul Aziz Abdullah, Wais Achyar, Catellia Auliany Agus Setiyono Akhmad Endang Zainal Hasan Alauddin, Daffa Rizal Dzulfaqaar Anisa Gianti Zhafira ANNA SETIADI RANTI Anna Setiadi Ranti Athaya, Ardelia Ayu Tri Nursyarah Aziz Syamsul Huda Aziz Syamsul Huda Bella Fatima Dora Zaelani C Hanny Wijaya Cika Hilda Fransiski Destiandani, Khansa Dewi Purnamasari Dimas Andrianto DIMAS ANDRIANTO Dinar Mutiara Hikmah Djarot Sasongko Hami Seno Dwicesaria, Maheswari Alfira Dwitasari, Oczhinvia Dwitasari, Oczhinvia Eka Wahyu Purnama Etik Mardliyati, Etik Eva Harlina Fachriyan H Pasaribu Faoziduhu Bawamenewi FARAH FAHMA Febrina, Adella Fernanda Chairunisa Fernanda Chairunisa Fibri Yuniasih, Tiara Fitria Tika Pradita Fitriana S Monisa Fitrilia, Tiana Gholam, Gusnia Meilin Hanif Alamudin Manshur Hasim Hasim Hasim Hendra Susanto Huda, Aziz Syamsul Hudayanti, Martini Husnawati Husnawati Husnawati, . Ilham Gilang Cahya Ahmada Imanniar Yuta Ellana Dittama Indariani, Susi Iriani Setyaningsih Irma Rahmayani Irsal, Riyan Alifbi Putera Kartika, Yayuk Kayla Faza Gustafri Mandalika Kustiariyah Tarman LAKSMI AMBARSARI Madyastuti, Rini Mala Nurilmala MARIA BINTANG Maria Bintang Marwanto, Yoga Dwi Maulana, Farhan Mere, Janrigo Klaumegio Meydia Meydia Miantika, Shafillah Mita Gebriella Inthe Monita Rekasih Muhammad Assyar Mustika Weni Mustika Weni Mustika Weni Mustopa, Syahrul Mutmainnah Agustiawan Umar Mutmainnah Umar Nabilla, Rahmah Neni Widowati Nirmala Peni Sugiharti Nur Azizah Nusyarah, Ayu Tri Oczhinvia Dwitasari Paramitha Wirdani Ningsih Marlina Pipih Suptijah Pipih Suptijah Prayoga Pannindriya Prayoga Pannindrya Purwanto, Ukhradiya Magharaniq Safira Puspa Julistia Puspita Putri, Azka Adzkya Emalia Putu Kristiani Kalontong Rahadian Pratama Rahmadi Ganesha Putri Rahmania, Fitriani Jati Raisyadikara, Fadila Rini Kurniasih, Rini Riska Ariyanti, Riska Riska Susila Putri Ritonga, Aprilita Putri Defan Riyan Alifbi Putera Irsal Riyan Alifbi Putera Irsal Rizsa Mustika Pertiwi Rori Theresia Rosalina Yuliani Rosyidah, Rara Annisaur Safira P, Ukhradiya Magharaniq Saputra, Rahadita Maura Amanda Sari, Sintia Permata SEDARNAWATI YASNI Septaningsih, Dewi Anggraini Septiyani, Dinie Silitonga, Ria Gusharani Sri Novita Sagita Sulistiyani Susanto, Hendra Susi Indariani Syaefudiin, Syaefudin Syaefudin Syaefudin Syaefudin Syamsul Falah Takeshi Katayama Theresia, Rori Tien Lastini Tjahja Muhandri Toshisada Suzuki Umar, Mutmainnah Agustiawan Uswatun Hasanah Vitriyanna Mutiara Yuhendri Wong, Chen Wai Yanti Fajarwati Yundari, Yundari Zaelani, Bella Fatima Dora Zatta Yumni Ihdhar Syarafina Zuraida Zuraida