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All Journal International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Global Medical and Health Communication Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry

Mieke Hemiawati Satari

Faculty of Dentistry, Universitas Padjadjaran

Author-ID : 374309

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Correlation between Protein-with-Molecular-Weight-53 (p53), Burkit Cell Lymphoma 2 (Bcl2), and Fas Ligand (FasL) and Vascular-Cell-Adhesion-Molecule-1 (VCAM-1) mRNA Expression Levels in a Pathogenesis Study of Preeclampsia Teguh, Mintareja; Karkata, Made Kornia; Wirakusumah, Firman Fuad; Mose, Johanes Cornelius; Satari, Mieke Hemiawati; Septiani, Leri
International Journal of Integrated Health Sciences Vol 2, No 1 (2014)
Publisher : International Journal of Integrated Health Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1346.304 KB)

Objective: To determine the role of protein-with-molecular-weight-53 (p53), burkit cell lymphoma 2 (Bcl2),Â Fas ligand (FasL) mRNA, and vascular cell adhesion molecule 1 (VCAM-1), known as the apoptosis-related molecular pathway, in preeclamptic patients. Â Â Â Methods: Observation on the correlation between the mRNA levels of p53, Bcl2 and FasL and VCAM-1 in 31 subjects at 28-42 weeks gestational age was performed in this study using the real time reverse transcriptase-polymerase chain reaction (RT-PCR).Â Â Â Results: The results showed that p53 mRNA increased (>1.2350 ng/Î¼L) in the preeclampsia group compared to the normal pregnancy group (p=0.010), Bcl2 mRNA was lower (â‰¤0.9271 ng/Î¼L) in the preeclampsia group than the control group (p=0.041). There was also a tendency of increased FasL mRNA expression (>0.5509 ng/Î¼L) in the preeclampsia group compared to the normal pregnancy group (p=0.300). The level of VCAM-1 elevated (>890.08 ng/mL) in the preeclampsia group compared to the normal pregnancy group (p=0.001). In preeclampsia, the correlation between the Bcl2/p53 ratio and VCAM-1 was r=0.541 (p=0.002), whereas the correlation in normal pregnancy was r=0.099 (p=0.595). Â Â Â Conclusions: There are correlations between the mRNA expression levels of p53 and Bcl2 as an intrinsic pathway of apoptosis along with the VCAM-1 levels in the incidence of preeclampsia. However, no correlation is found between FasL mRNA expression and the incidence of preeclampsia.Keywords: Bcl2, FasL, p53, Preeclampsia, VCAM-1DOI: 10.15850/ijihs.v2n1.273

AKTIVITAS ANTIJAMUR FRAKSI AIR SARANG SEMUT MYRMECODIA PENDENS PADA CANDIDA ALBICANS ATCC 10231 Balafif, Felisha Febriane; Satari, Mieke Hemiawati; Dhianawaty, Diah
Majalah Kedokteran Bandung Vol 49, No 1 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Penggunaan tanaman herbal untuk pengobatan dan pencegahan penyakit semakin populer sehingga penelitian mengenai senyawa aktif dari tumbuhan yang berkhasiat semakin menjadi perhatian. Umbi sarang semut (Myrmecodia pendens) mengandung senyawa aktif berupa terpenoid, tanin, fenol, flavonoid, dan saponin yang memiliki efek antijamur pada Candida albicans. Penelitian ini bertujuan mengukur konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) fraksi air M. pendens dan menguji perbedaan efek antijamur antara fraksi air M. pendens dan nistatin pada C. albicans ATCC 10231. Penelitian dilaksanakan pada bulan Juni?Juli 2015 di Laboratorium Penelitian Jurusan Kimia Fakultas MIPA Universitas Padjadjaran. Penelitian ini menggunakan metode mikrodilusi dan nilai KHM diukur dengan enzyme linked immunosorbent assay reader dan nilai KBM diukur dengan pengujian pada media agar. Data dianalisis menggunakan uji-t dengan level signifikan p<0,05 untuk menentukkan perbedaan efek antijamur fraksi air M. pendens dengan nistatin pada C. albicans. Hasil penelitian menunjukkan bahwa nilai KHM ialah 1.250 ?g/mL dan nilai KBM ialah 2.500 ?g/mL. Simpulan penelitian ini ialah nilai KHM serta KBM fraksi air M. pendens ialah 1.250 dan 2.500 ?g/mL dan terdapat perbedaan efek antijamur antara fraksi air dan nistatin terhadap C. albicans. [MKB. 2016;49(1):28?34]Kata kunci: Candida albicans, fraksi air, konsentrasi bunuh minimum, konsentrasi hambat minimum, Myrmecodia pendensAntifungal Activity of Ant Hill Myrmecodia Pendens Water Fraction against Candida Albicans ATCC 10231The use of herbal plant for treatment and prevention of diseases is getting more popular, emphasizing the need for studies on active compounds from plants. Ant hill (Myrmecodia pendens) contains active compounds such as terpenoid, tannin, phenol, flavonoid, and saponin which have antifungal effects on Candida albicans. The objectives of the study were to measure the value of minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of water fraction of M. pendens and antifungal effect of water fractions of M. pendens against C. albicans compared to nystatin. This study used microdilution and the effects were measured with enzyme linked immunosorbent assay reader to determine MIC value, followed by agar media assay to determine MFC. Data were analyzed using T test with significant level p < 0.05 to determine antifungal effect of water fractions of M. pendens against C. albicans compared to nystatin. The result showed that MIC value was 1.250 ?g/ml and MFC value was 2.500 ?g/ml. T test showed significant difference of % inhibition cells growth effect between M. pendens water fraction and nystatin (p=0.014 < 0.05). It is concluded that the M. pendens water fraction has an antifungal effect against C. albicans with MIC and MFC values of 1.250 and 2.500 ?g/ml.There are differences in antifungal effects between water fraction of M. pendens and nystatin against C. albicans. [MKB. 2016;49(1):28?34] Key words: Candida albicans, minimum fungicidal concentration, minimum inhibitory concentration, Myrmecodia pendens, water fraction

The Effect of Mixed-Fruit Juice on Uterine Contractions and Cervical Dilatation During the First Stage of Delivery Martasari, Bellia Loranthifolia; Cahyadi, Wisnu; Nugraha, Gaga Irawan; Husin, Farid; Susiarno, Hadi; Hidayat, Yudi Mulyana; Satari, Mieke Hemiawati
Global Medical & Health Communication (GMHC) Vol 7, No 1 (2019)
Publisher : Universitas Islam Bandung

Energy imbalance in delivery can inhibit the action of glycolytic enzymes and interfere with chemical reactions in muscle cells. These nuisances may interfere with uterine contractions that obstruct cervical dilatation. Therefore, mothers require a nutritional alternative which is practical, generates energy quickly and supplies glucose needed for uterine contractions. These can be fulfilled with a mixed-fruit juice beverage. Mixed-fruit juice consists of fruits, Tunisian dates, honey, and red beans. This study aims to analyze the effect of the mixed-fruit extract on uterine contraction and cervical dilatation during the first stage of delivery. This study used a randomized controlled trial design. The target population was all the mothers who would give birth in Bandung city in March−April 2017. The samples of this study were the gravida <4 who would give birth at the Pelayanan Obstetri Neonatal Emergensi Dasar/PONED (Basic Emergency Obstetric and Neonatal Care/BEONC) Puskesmas (Public Health Center) Ibrahim Ajie, Puter, Garuda, Pagarsih, and Padasuka, consisting of 30 subjects as the treatment group and other 30 subjects as the control group. Uterine contractions and cervical dilatation were measured clinically and recorded on partograph. The analysis of data was done using the chi-square test, independent t test, and Mann-Whitney test. The results showed that there was the effect of mix-juice on the frequency, the duration and the intensity of uterine contractions and cervical dilatation with p value<0.05 and relative risk (RR) values respectively of 1.3, 3.3, 2.6, 1.7. In conclusion, consuming mixed-fruit juice during the first stage of delivery give a significant impact on the progress of uterine contractions and cervical dilatation.PENGARUH PEMBERIAN MINUMAN MIX JUICE TERHADAP KONTRAKSI UTERUS DAN PEMBUKAAN SERVIKS SELAMA KALA I PERSALINANKetidakseimbangan energi saat persalinan dapat menghambat kerja enzim glikolitik dan mengganggu reaksi kimia dalam sel otot sehingga dapat menghambat kontraksi otot dan pembukaan serviks. Perlu alternatif nutrisi ibu bersalin yang praktis, cepat menghasilkan energi, dan memberikan asupan glukosa yang dibutuhkan untuk kontraksi uterus dalam bentuk minuman mix juice. Mix juice ini mengandung buah-buahan, kurma tunisia, madu, dan kacang merah. Penelitian ini bertujuan menganalisis pengaruh pemberian minuman mix juice terhadap kontraksi uterus dan pembukaan serviks selama kala I persalinan. Penelitian ini menggunakan desain randomized controlled trial. Populasi target adalah semua ibu yang akan melahirkan di Kota Bandung pada bulan Maret−April 2017. Sampel dalam penelitian ini adalah gravida <4 yang akan melahirkan di Puskesmas Pelayanan Obstetri Neonatus Emergensi Dasar (PONED) Ibrahim Ajie, Puter, Garuda, Pagarsih, dan Padasuka, yaitu 30 subjek pada kelompok perlakuan dan 30 subjek pada kelompok kontrol. Kontraksi uterus dan pembukaan serviks diukur secara klinis dan dicatat pada partograf. Analisis data menggunakan uji chi-kuadrat, uji t independen, dan Uji Mann-Whitney. Hasil penelitian didapatkan pengaruh pemberian minuman mix juice terhadap frekuensi, lama dan intensitas kontraksi uterus, serta pembukaan serviks dengan nilai p<0,05 dan nilai RR masing-masing sebesar 1,3; 3,3; 2,6; 1,7. Simpulan, pemberian minuman mix juice selama kala I persalinan berpengaruh terhadap kemajuan kontraksi uterus dan pembukaan serviks.

Antifungal effect of ethyl acetate fraction of Sarang semut (Myrmecodia pendens Merr.& Perry) against Candida albicans Balafif, Felisha Febriane; Satari, Mieke Hemiawati; Dhianawaty, Diah
Padjadjaran Journal of Dentistry Vol 34, No 3 (2022): November 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no3.36703

ABSTRACTIntroduction: Oral candidiasis is a fungal infection of the oral cavity that is caused by Candida albicans (C. albicans). Treatment of oral candidiasis usually uses topical agents such as nystatin, but it comes with side effects. Research on medicinal materials from plants can be an alternative to chemical drugs. It has increasingly become a concern such as the tuber of sarang semut (Myrmecodia pendens Merr. & L.M. Perry). The aim of the study is to determine the effect of ethyl acetate and hexane fractions of M. pendens against C. albicans. Methods: M. pendens methanol extract used maceration method. As much as 33 g concentrated M. pendens methanol extract was partitioned with a separating funnel based on the polarity of the substances to obtain ethyl acetate fraction. The minimum inhibitory concentration (MIC) procedure was carried out by the microdilution method and measured by an ELISA reader. The minimum fungicidal concentration (MFC) was obtained conclusively on Mueller Hinton agar media at concentrations above MIC of ethyl acetate fraction of M. pendens. Results: Ethyl acetate fraction had antifungal effect against C. albicans. The MIC and MFC of ethyl acetate fraction were 625 μg/ml and 1.250 μg/ml. Conclusion: Ethyl acetate fraction of M. pendens can inhibit the growth of C. albicans. M. pendens can be developed as the prevention agent of oral candidiasis.Keywords: antifungal; candida albicans; ethyl acetate fraction; Myrmecodia pendens

The antimicrobial effect of piper betel leaves extract against Streptococcus mutans Mohammad Subri, Liyana; Dewi, Warta; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 24, No 3 (2012): November 2012
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol24no3.26835

Introduction: Betel leaves are herbal plant that is believed to have phenolic compound as an antimicrobial agent. One of the medical plants (herbal) which has an-timicrobial properties is Piper betel.The aim of this research is to analyzed the antimicrobial effect of Piper Betel Leaves extract against Streptococcus mutans. Methods: Type of this research is laboratory experiment. Population of this study are Piper betle Linn plant in West Java. The MIC test were conducted based on serial dilution method in 64%, 32%, 16%, 8%, 4%, 2%, 1%, 0.5%, 0.25%, 0.125% concentration of Piper betel leaves extracts against 5 sample of Streptococcus mutans within three repetitions. Results: The result shows that 0.25% concentration of Piper betel leaves extract can inhibit Streptococcus mutans. Conclusion: Piper betel leaves extract can inhibit the growth and had antimicrobial effect against Streptococcus mutans.

Bacterial inhibition test of methanolic extracts of strawberry (Fragraia x ananassa Duchesne), lime (Citrus aurantifolia), and radish (Raphanus sativus L.), towards Streptococcus Sanguis ATCC 10556 Anggraini, Fitri; Satari, Mieke Hemiawati; Mariam, Marry Siti
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July 2018
Publisher : Universitas Padjadjaran

Introduction: Caries are initiated by the plaque formation on the tooth surface, due to the interaction between food debris and bacteria in the mouth. The pioneer bacterium of plaque formation is Streptococcus sanguis. The Strawberry fruit, lime fruit, and radish root are part of the plant that contains antibacterial substances. Flavonoid, tannin, saponin, alkaloid, polyphenol, terpenoid and quinon as antibacterial substances. This study aimed to evaluate strawberry fruit, lime fruit, and radish root methanol extract can inhibit Streptococcus sanguis ATCC 10556. Therefore, strawberry, lime, and radish can be made into mouthwash, bubble gum, or toothpaste to prevent plaque formation. Methods: This study used a Kirby-Bauer diffusion test as the inhibition test. Results: The result of this study that the biggest inhibition zone was lime methanol extract 20,000 ppm, and the smallest one was radish methanol extract 5,000 ppm. Conclusion: Strawberry fruit, lime fruit peel, and radish root methanol extract and their combination had the effect of inhibiting bacterial growth synergistically against Streptococcus sanguis ATCC 10556.

Antibacterial effect of clove (Eugenia aromaticum) oil extracted from clove cigarettes towards Streptococcus mutans Jumali, Ahmad Alif Wazir; Satari, Mieke Hemiawati; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 25, No 1 (2013): March 2013
Publisher : Universitas Padjadjaran

Streptococcus mutans is a commensal bacteria of the oral cavity, particularly found in dental plaque attached to the tooth surface, and can also found in the saliva, buccal mucosa, tongue, and the gingival sulcus. Clove cigarette contains the clove oil used worldwide as a herbal remedy for a variety of health disorders due to its antibacterial, antifungal, and antiviral properties. The purpose of this study was to determined the antibacterial effect of the clove oil contained in the clove cigarette towards Streptococcus mutans as a cariogenic bacteria. The research was an experimental laboratory, which tested the sensitivity of the Streptococcus mutans bacteria taken from the saliva of 10 clove cigarette smokers towards the clove oil extract of the clove cigarette in-vitro by using the Kirby-Bauer method. The study showed that the clove oil extract of the clove cigarette which contained eugenol has antibacterial towards the growth of Streptococcus mutans, and there was a difference of the antibacterial activity between clove oil extracted from minced and combusted clove cigarette. The conclusion of this study was the clove oil extracted from minced clove cigarette had a better antibacterial effect than the combusted clove cigarette.

Antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers towards Streptococcus sanguis ATCC 10566 Fatriadi, Fajar; Kurnia, Dikdik; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Universitas Padjadjaran

Introduction: Streptococcus sanguis is an initial cause of dental plaque formation which is the initial cause of caries. One of the preventive treatments can be done by using the mouthwash containing antibacterial substances. Along time, natural remedies are proven to be having more antibacterial properties. Ant-plant (Myrmecodia pendens Merr. & Perry) tubers are types of epiphytic plant that grows in many parts of Papua, with many health benefits, and are known to contain flavonoids, tannins, and tocopherols. This study was aimed to determine the antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers on the growth of Streptococcus sanguis ATCC 10566. Methods: The ant-plant tubers were extracted with soxhletation method using the methanol solvent. The fractionation was then performed using ethyl acetate to obtain the ethyl acetate fraction. Result: The phytochemical test showed that the ethyl acetate fraction of the ant-plant contained phenolics, tannins, flavonoids, and terpenoids. The bacterial test in this study was using the microdilution method using the ELISA Reader by measuring the Minimum Inhibitory Concentration (MIC) of ethyl acetate fraction of ant-plant tubers towards Streptococcus sanguis with positive control was using the chlorhexidine gluconate as the golden standard medication. The ethyl acetate fraction of ant-plant tuber had an antibacterial effect towards Streptococcus sanguis in the MIC of 31.25 ppm, while chlorhexidine gluconate was 0.49 ppm. Conclusion: The ethyl acetate fraction of ant-plant had a lower antibacterial activity compared to chlorhexidine gluconate on the growth of Streptococcus sanguis ATCC 10566.Keywords: Ant-plant tubers, Myrmecodia pendens Merr. & Perry, antibacterial activity, Streptococcus sanguis ATCC 10566.

The inhibitory effectiveness of ethanol extract of selasih leaves towards Streptococcus sanguis growth Susilo, Ivhatry Rizky Octavia Putri; Satari, Mieke Hemiawati; Setiawan, Ame Suciati
Padjadjaran Journal of Dentistry Vol 27, No 1 (2015): March 2015
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol27no1.26694

Introduction: Selasih is one of tropical and subtropical plant used as traditional medicine. It has antibacterial activity especially toward Gram-positive bacteria because it contains volatile oils, tannin, flavonoid and tertepenoid. Method: The study was performed as an experimental laboratory study used Kirby Bauer agar diffusion applied to eight samples of Streptococcus sanguis which was done by twice repetition for each sample. The concentrations of ethanol extract were 16%, 8%, 4%, 2% and 1%.The result was analysed using ANOVA method for single factor of experimental design. The result of the study showed that there was evidence of the antibacterial effect contained in the ethanol extract of selasih leaves inhibiting the growth of Streptococcus sanguis . The average of inhibitory zone for each concentration in every sample, were: 5,25 mm for 16%, 3,22 mm for 8%, 1,84 mm for 4%, 1,53 mm for 2% and 0,19 mm for 1%. The conclusion of the study indicates that there is an antibacterial effects in ethanol extract of selasih leaves (Ocimum basilicum Linn) which inhibits the growth of Streptococcus sanguis. This antibacterial strength is caused by the active content of selasih leaves extract.Conclusion: The extract of basilicum ethanol (Ocimum basilicum L) effectively inhibits the growth of Streptococcus sanguis.

Anticancer potency of black sea cucumber (Holothuria atra) from Mentawai Islands, Indonesia Satari, Mieke Hemiawati; Arma, Utmi; Ilyas, Syafruddin; Handayani, Dian
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March 2017
Publisher : Universitas Padjadjaran

Introduction: The source of bioactive compounds believed to have strong anticancer potency is derived from sea cucumber. Black sea cucumber (Holothuria atra) is a dominant species in Mentawai Islands, West Sumatera, Indonesia. Key factor compound that acts as anticancer in sea cucumber extract is tritepenoid also known as Frondoside A. The purpose of this study was to determine the effectiveness of the active compound taken from black sea cucumber as anticancer. Methods: Methods used was experimental laboratory. Anticancer activity of black sea cucumber was determined by using the MTT assay cytotoxic test to measure the IC50. Cytotoxic test was conducted with Supris clone-1 (SP-C1) derived from cloned lymphadenopathy of patients with oral squamous cell carcinoma on lingual part. Results: It was found that the compounds contained in black sea cucumber has strong anticancer activity (IC50 = 5.25 μL/mL) against SP-C1. Conclusion: Black sea cucumber extract has strong cytotoxic effects against cancer cells SP-C1, therefore black sea cucumber extract potentially developed as a new source of cancer medicine.

Co-Authors Ame Suciati Setiawan Apriyanti, Eria Ariningtyas Asri, Lia Amelia Tresna Wulan Balafif, Felisha Febriane Bambang Pontjo Priosoeryanto Bambang Sunendar Purwasasmita, Bambang Sunendar Bethy Suryawathy Hernowo bin Jumali, Ahmad Alif Wazir Bremmy Laksono Budiani, Yona Ayumi Dahlia Sutanto Diah Dhianawaty Dian Handayani Dikdik Kurnia Edeh Rolleta Fajar Fatriadi Firman Fuad Wirakusumah Fitri Anggraini, Fitri Gaga Irawan Nugraha Gita Dwi Jiwanda Sovira Hajanajumudin, Hanizul Harun Achmad, Harun Husin, Farid Imelda, Reza Afri Inmawaty, Jesieca Inne Suherna Sasmita Jasrin, Tadeus Arufan Johanes C. Mose Jumali, Ahmad Alif Wazir Leri Septiani Loon, Yong Ker Loon, Yong Ker Made Kornia Karkata Maria Aurora Titiana, Maria Aurora Marry Siti Mariam Martasari, Bellia Loranthifolia Melani, Intan Mintareja Teguh Moch . Rodian Mohammad Subri, Liyana Rifki Septawendar Roosje Rosita Oewen, Roosje Rosita Sudjarwo, Indrati Supriatno Susiarno, Hadi Susilo, Ivhatry Rizky octavia putri Syafruddin Ilyas Utmi Arma, Utmi Warta Dewi, Warta Yetty Herdiyati Nonong, Yetty Herdiyati Yudi Mulyana Hidayat Yuti Malinda

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