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All Journal International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Global Medical and Health Communication Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry

Mieke Hemiawati Satari

Faculty of Dentistry, Universitas Padjadjaran

Author-ID : 374309

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Antibacterial effect of pineapple (Ananas comosus) extract towards Staphylococcus aureus Loon, Yong Ker; Satari, Mieke Hemiawati; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Staphylococcus aureus is one of the important medical pathogens which have been recognised for many years as a remedyfor a wide case of oral infections. Nowadays, the use of herbal remedy for reducing bacteria in the oral cavity has been implemented widely due to thefewer side effects. Therefore, researchershave been findingwaysto use pineapple in dentistry to prevent many cases of oral diseases. The purpose of this study was to prove that pineapple extract indifferent concentration had the potential as an antibacterial agent towards Staphylococcus aureus. Methods: The study was an experimental laboratory conducted by determining the minimum inhibitory concentration of pineapple (Ananas comosus) with thetwo-fold serial dilution methods. Results:The The Minimum Inhibitory Concentration (MIC) of the pineapple extract was 1.56%-0.78%. Conclusion: The pineapple extract had anantibacterial effect towards Staphylococcus aureus due to the bromelain compound and its phytochemical factor such as Vitamin C and flavonoid.

Effect of cassava (Manihot esculenta cranzt), rice (Oryza sativa L.), and potato (Solanum tuberosum) water extract to decrease pH phase fermentation of Streptococcus mutans ATCC 25175 Imelda, Reza Afri; Mariam, Marry Siti; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Dental caries is the localised damage of the hard tooth tissue caused by acid production results of carbohydrate fermentation by acid-producing bacteria. Streptococcus mutans are one of the aetiological bacteria which fermented carbohydrate causes a decrease in the oral pH. Carbohydrate is generally consumed, included cassava, rice, and potato. The purpose of this study was to determine the mechanism of the water extract of rice, cassava, and potato in decreasing the pH of Streptococcus mutans culture to know their potential in causing caries and also determined decreasing differences between three water extracts. Methods: The experimental method was used in cultured Streptococcus mutans ATCC 25175 and tested against the treated rice, cassava, and potato water extract. Cultured bacteria were incubated for 48 hours in facultative anaerobes then measured the pH with a digital pH meter. The measurement result was statistically tested with the paired t-test and ANOVA. Results: The paired t-test (α = 0.05) showed that the p-value of rice, cassava, and potato water extract were 0.001, 0.001, and 0.018 respectively. ANOVA test with an α value of 0.05 showed the p-value of 0.000. Conclusion: There was a decrease in pH of cultured Streptococcus mutans after administration of the water extract of cassava, rice, and potato, and there was a difference between the pH decrease of the Streptococcus mutans between administration of water extract of rice, cassava, and potato. Keywords: Streptococcus mutans, Manihot esculentacrantz, Oryza sativa L., Solanum tuberosum, pH phase fermentation

In vivo histomorphological evaluation of geopolymer-carbonated apatite nanocomposites implanted on rabbit tibia at early bone healing Sutanto, Dahlia; Satari, Mieke Hemiawati; Hernowo, Bethy Suryawathy; Priosoeryanto, Bambang Pontjo; Septawendar, Rifki; Asri, Lia Amelia Tresna Wulan; Purwasasmita, Bambang Sunendar
Padjadjaran Journal of Dentistry Vol 33, No 1 (2021): March 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no1.28899

Introduction: Dental implants have become a more desirable treatment for replacing missing teeth. The mechanical properties and biocompatibility of titanium and zirconia are excellent, but they are less bioactive. The chemical composition of the carbonate apatite is similar to enamel and dentin. Geopolymers are inorganic polymers, and they are similar to ceramics. They have excellent mechanical properties, bioactivity, biocompatibility. The purpose of this study was to assess histomorphological evaluation of geopolymer-carbonated apatite nanocomposites implanted on rabbit tibia at early bone healing in vivo. Methods: Geopolymer-CHA nanocomposites with a diameter of 3 mm and length of 6 mm was placed in the tibia of eight male New Zealand White rabbit whose body weight is 3 to 3.5 kg and six-month ages. Experimental subjects were randomly assigned to 2 groups for assessing the bone healing capability around samples to 14 and 28 days histomorphologically. Wilcoxon test was performed, and p<0.05 was considered significant, using Minitab software version 13. Results: Granulation tissue, woven, and lamellar bone was analysed. A reactive bone formation was revealed in the 14th day. Osteoblasts, osteoids, and osteocytes showed more mature and woven bone became denser on the 28th day. Conclusion: Geopolymer-CHA nanocomposites could be considered a candidate for dental implant material from this histomorphological evaluation.

Inhibitory concentrations of gambier (Uncaria gambir Roxb.) catechins extract against Streptococcus mutans Inmawaty, Jesieca; Sudjarwo, Indrati; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 24, No 3 (2012): November 2012
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol24no3.26832

Introduction: Catechin which extracted from gambir (Uncaria gambir Roxb) is a major component of polyphenol compounds. The catechins compound acts as an antibacterial. The study was to analyze the inhibitory concentration gambir catechin extract against Streptococcus mutans as the bacteria that play a role in the formation of dental caries. Methods: The study was conducted in a laboratory experiment by testing inhibitory concentration gambir catechin extract, through Kirby Bauer disk diffusion method on plates TYCSB for 1 x 24 hours. Test bacteria Streptococcus mutans isolated from saliva. The used suspension of bacteria was made according to the standard turbidity of Mc Farland 0.5 are each 1 ml suspension containing 1.5 x 108 bacteria. The powder obtained from catechin gambir extract through freezing method. Results: Catechins concentrations of 20% produces the lowest inhibition, with an inhibitory diameter of 0,615 cm. The largest inhibition produces by the concentration of catechins with 80% inhibition at 1,085 cm inhibitory diameter. Conclusion: The higher concentration of catechins, the greater inhibition produces. Conversely, the lower concentration of catechins will be lower inhibition produces. the resulting zone of inhibition will be smaller.

Antimicrobial properties of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis ATCC 29212 Sovira, Gita Dwi Jiwanda; Satari, Mieke Hemiawati; Mariam, Marry Siti
Padjadjaran Journal of Dentistry Vol 33, No 1 (2021): March 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no1.25206

Introduction: Enterococcus faecalis is often found in root canal infections and can cause failure in root canal treatment. Uncaria gambier is an herbal medicine that is known to be rich in polyphenol compounds that have antibacterial properties. Because of the polyphenol content and antibacterial properties of Uncaria gambier, it can be an alternative as an antibacterial solution for root canal treatment. The purpose of this study is to analyze the antimicrobial of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis (ATCC 29212). Methods: This study is descriptive explorative research Uncaria gambier was extracted using a combination of three different fractions, ethyl acetate-water, hexane-water and hexane-ethyl acetate. We determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against Enterococcus faecalis (ATCC 29212) using the serial microdilution method and by add test samples to the Mueller Hinton agar medium from each fraction Results: Combination Ethyl Acetate-water has MIC value at 0.195 mg/ml, combination Hexane-water at 0.049 mg/ml and combination Hexane- Ethyl Acetate have the MIC value at 1.563 mg/ml. The MBC value in combination Ethyl Acetate-water at 25 mg/ml, combination Hexane-Ethyl Acetat and Hexane-water MBC value at 50 mg/ml and 100 mg/ml. Based on optical density measurement using ELISA Reader and bacterial growth on the media used, the fraction combinations increase the antibacterial effect of Uncaria gambier against E. faecalis ATCC 29212. Conclusion: The combination of various Uncaria gambier solvents has been shown to inhibit and kill E. faecalis (ATCC 29212). The combination of Ethyl Acetate-water fraction is the best combination to against E. faecalis (ATCC 29212). It means that the combination of various Uncaria gambier solvents can be developed as alternative root canal irrigation.

Antibacterial effect of cinnamon essential oil (Cinnamon cassia) in different concentration towards Streptococcus sanguis Hajanajumudin, Hanizul; Satari, Mieke Hemiawati; Setiawan, Ame Suciati
Padjadjaran Journal of Dentistry Vol 22, No 2 (2010): July 2010
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol22no2.26845

Introduction: Streptococcus sanguis is a type of normal bacteria found in the oral cavity, particularly in plaque on tooth surfaces and can also be found in saliva, buccal mucosa, tongue and gingival sulcus. At present, the usage of herbal remedy for reducing plaque in oral cavity has been implemented widely and one of the herbal remedy used is cinnamon essential oil. The purpose of this study is to prove that there are differences in the antibacterial activity of cinnamon essential oil with different concentrations against Streptococcus sanguis. Methods: The study was performed using laboratory experiments, in which the sensitivity of the Streptococcus sanguis bacteria from the saliva of 10 KPBI batch 2006 dental students were tested in vitro against cinnamon essential oil by using Kirby-Bauer method. Results: The study shows that the cinnamon essential oil has different antibacterial abilities in inhibiting the growth of Streptococcus sanguis in different concentrations. This is due to the element cinnamaldehyde and eugenol that they posses as their main constituent. There is a significant difference between the antibacterial effects of Cinnamon cassia essential oil in different concentrations towards Streptococcus sanguis. Conclusion: Cinnamaldehyde and eugenol has been proved to have antibacterial effect towards wide variety of bacteria including Streptococcus sanguis.

The effect of ethanol extract of mangostene (Garcinia Mangostana Linn.) peel on tongue cancer cells Supri's Clone-1 apoptosis, in vitro Titiana, Maria Aurora; Oewen, Roosje Rosita; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November 2011
Publisher : Universitas Padjadjaran

Apoptosis or programmed cell death serves to regulate physiological and pathological conditions. Garcinia mangostana Linn. is one of the medical herbs that is widely used to suppress human tongue cancer growth by inducing apoptosis. The research aimed to analyze the effect of ethanol extract of Garcinia mangostana Linn. on tongue cancer cells apoptosis Supri's Clone-1 (SP-C1). The type of the research was an experimental laboratory. Tongue cancer cells SP-C1 were treated by various ethanol extract concentrations (0, 300, 400, 500, 600, 700 pg/ml) of Garcinia mangostana Linn. to detect the apoptosis, which was done by acridine orange and ethidium bromide coloring tests. The number of tongue cancer cells SP-C1 2x104 cells/dish. Observation on cells apoptosis was observed by a fluorescent microscope with 40 x magnification. The data were analyzed using one-way ANOVA and was followed by Post Hoc test (Tukey-test) with 95% significance level. The result showed that tongue cancer cells SP-C1 apoptosis treated by various ethanol extracts of Garcinia mangostana Linn. increase significantly. The highest effect of apoptosis was detected at the 700 pg/ml concentration that has an effect on the percentage of tongue cancer cells apoptosis by 65%.

Role of Streptococcus Anginosus on the formation of dental caries Nonong, Yetty Herdiyati; Satari, Mieke Hemiawati; Sasmita, Inne Suherna
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November 2011
Publisher : Universitas Padjadjaran

Generally, the etiology of dental caries is the cariogenic properties of bacteria, these are always associated with Streptococcus mutans. Glucosyltransferase fragment (Gtf) are also in other strains of Streptococcus such as Streptococcus anginosus, Streptococcus milleri which includes beta hemolysis. Genotypically B Streptococcus anginosus has genetic characteristics that are similar to Streptococcus mutans. The research objective was to determine the existence of Gtf B/C gene as a cause of caries in Streptococcus anginosus. The study was conducted in experimental laboratories with PCR technique by taking a sample of 20 children who had caries. The results showed there was the amplification of Streptococcus anginosus with a level of homology 96%, 97%, and 99%. The results of the Gtf genes amplification fragment B/C provided 600 pb ribbon. The conclusion was Streptococcus anginosus classified as cariogenic bacteria because they had Gtf B/C genes.

Co-Authors Ame Suciati Setiawan Apriyanti, Eria Ariningtyas Asri, Lia Amelia Tresna Wulan Balafif, Felisha Febriane Bambang Pontjo Priosoeryanto Bambang Sunendar Purwasasmita, Bambang Sunendar Bethy Suryawathy Hernowo bin Jumali, Ahmad Alif Wazir Bremmy Laksono Budiani, Yona Ayumi Dahlia Sutanto Diah Dhianawaty Dian Handayani Dikdik Kurnia Edeh Rolleta Fajar Fatriadi Firman Fuad Wirakusumah Fitri Anggraini, Fitri Gaga Irawan Nugraha Gita Dwi Jiwanda Sovira Hajanajumudin, Hanizul Harun Achmad, Harun Husin, Farid Imelda, Reza Afri Inmawaty, Jesieca Inne Suherna Sasmita Jasrin, Tadeus Arufan Johanes C. Mose Jumali, Ahmad Alif Wazir Leri Septiani Loon, Yong Ker Loon, Yong Ker Made Kornia Karkata Maria Aurora Titiana, Maria Aurora Marry Siti Mariam Martasari, Bellia Loranthifolia Melani, Intan Mintareja Teguh Moch . Rodian Mohammad Subri, Liyana Rifki Septawendar Roosje Rosita Oewen, Roosje Rosita Sudjarwo, Indrati Supriatno Susiarno, Hadi Susilo, Ivhatry Rizky octavia putri Syafruddin Ilyas Utmi Arma, Utmi Warta Dewi, Warta Yetty Herdiyati Nonong, Yetty Herdiyati Yudi Mulyana Hidayat Yuti Malinda

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