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All Journal AGRIKA Cropsaver : Journal of Plant Protection Agridevina : Berkala Ilmiah Agribisnis Plumula : Berkala Ilmiah Agroteknologi Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Ilmu-Ilmu Pertanian Indonesia Pelita Perkebunan JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA International Journal of Applied Biology Agriprima : Journal of Applied Agricultural Sciences BIOEDUSCIENCE Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Abadimas Adi Buana Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Agro Bali: Agricultural Journal Jurnal Layanan Masyarakat (Journal of Public Service) Biofarm : Jurnal Ilmiah Pertanian Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Nusantara Science and Technology Proceedings Multidiciplinary Output Research for Actual and International Issue (Morfai Journal) Journal of Applied Agricultural Science and Technology Jurnal HPT (Hama Penyakit Tumbuhan) Eduvest - Journal of Universal Studies Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Sri Wiyatiningsih
Program Studi Agroteknologi, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya
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Effect of Fobio on Intensity of Moler Disease (Fusarium oxysporum) on Various Shallot Cultivars Elvira, Nolla Dwi; Wiyatiningsih, Sri; Suryaminarsih, Penta
CROPSAVER Vol 6, No 2 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i2.45747

Abstract

Fusarium oxysporum is the pathogen that causes moler disease which can reduce the productivity of shallot plants. The use of cultivars greatly affects the production. Control method that is more environmentally friendly is currently being pursued towards organic farming. Fobio is a biopesticide containing plant root microorganisms as biological control agents. This study aims to determine the effect of Fobio biopesticide on Fusarium wilt (moler) disease incidence and production of three shallot cultivars. The experiment was conducted in the shallot planting area of Sukorejo Village, Rejoso District, Nganjuk Regency, East Java. This experiment was arranged in the split plot design with two factors. The main plot of the concentration of the biopesticide formula (Fobio) consisted of 3 levels namely control (chemical fungicide), 5 ml/liter, and 10 ml/liter, and the subplot of shallot cultivar consisted of 3 types namely Tajuk, Biru Lanchor, and Super Philip. The observation in this study were the incubation period, disease symptoms, disease intensity, and shallot bulb yield. The results showed that Fobio biopesticide at the concentration of 10 ml/liter caused the same disease suppression as chemical fungicide (control) in the three shallot cultivars with the disease incidence of 18.78%. Fobio biopesticide concentration of 10 ml/liter had also the same effect as chemical fungicide on the production of those shallot cultivars, with the average wet weight of 2.25 kg/treatment and dry weight of 1.49 kg/treatment.
Biopestisida (FOBIO) Sebagai Penghambat Perkembangan Penyakit Layu Fusarium Pada Tanaman Pisang Di Provinsi Bali: Biopesticide (FOBIO) as an Inhibitor of Fusarium Wilt Disease Development in Banana Plants in Bali Province Rosyiidah Sholihin, Alyaa Arwaa; Wiyatiningsih, Sri; Suryaminarsih, Penta
Biofarm : Jurnal Ilmiah Pertanian Vol. 21 No. 1 (2025): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pisang (Musa paradisiaca L.) merupakan salah satu kebutuhan pokok bagi masyarakat Bali, terutama umat Hindu, yang mendorong pengembangan budidaya secara intensif di berbagai jenis lahan. Namun, pengembangan ini dihadapkan pada kendala penyakit layu fusarium yang disebabkan oleh Fusarium oxysporum f.sp. cubense. Penelitian ini mengevaluasi efektivitas biopestisida FOBIO dalam menekan intensitas serangan fusarium serta meningkatkan pertumbuhan tunas pisang. Penelitian dilakukan menggunakan Rancangan Acak Kelompok Faktorial (RAKF) dengan dua faktor: ketinggian tempat (650-950 mdpl dan 25-40 mdpl) serta konsentrasi biopestisida (kontrol dan FOBIO 7,5 ml/liter). Hasil penelitian menunjukkan bahwa aplikasi FOBIO 7,5 ml/liter mampu menurunkan persentase serangan dari kategori berat menjadi sedang sebesar 18,9%. Serangan tertinggi terjadi di dataran tinggi (650-950 mdpl) dengan intensitas 34,1% (kategori berat), di mana FOBIO tidak efektif dalam menurunkan intensitas serangan. Meski tidak signifikan, aplikasi FOBIO meningkatkan jumlah tunas baru sebesar 8,4 tunas di dataran tinggi.
PENGARUH BEBERAPA KONSENTRASI Streptomyces sp. TERHADAP LAJU PERTUMBUHAN Sclerotium rolfsii PENYEBAB PENYAKIT BUSUK BATANG TANAMAN KACANG TANAH (Arachis hypogaea L.) Ersyaf, Ihza Muhammad; Mujoko, Tri; Wiyatiningsih, Sri
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.6844

Abstract

ABSTRAK Sclerotium rolfsii adalah salah satu patogen yang tergolong penting pada tanaman kacang tanah. Upaya pengendalian penyakit ini dapat dilakukan dengan memanfaatkan kandungan senyawa antibiotik Streptomyces sp. Tujuan penelitian ini adalah mengetahui pengaruh penghambatan Streptomyces sp. terhadap S. rolfsii. Penelitian menggunakan rancangan acak lengkap (RAL) skala in-vitro dan in-vivo dengan perlakuan konsentrasi 5%, 10%, 15%, 20%, dan 25%. Hasil penelitian menunjukkan adanya pengaruh perlakuan konsentrasi Streptomyces sp. terhadap pertumbuhan S. rolfsii pada skala in-vitro tetapi pada skala in-vivo aplikasi perlakuan belum sepenuhnya optimal. Aplikasi 25% menunjukkan hasil terbaik pada seluruh parameter dibanding konsentrasi lainnya pada skala in-vitro dan in-vivo.
POTENSI BIOPESTISIDA FOBIO DALAM MENEKAN PENYAKIT BERCAK DAUN (Helminthosporium oryzae) PADA TANAMAN PADI GOGO ORGANIK DI MOJOKERTO Wiyatiningsih, Sri; Eben, Philipus Wahyu Baskoro; Mujoko, Tri
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.7358

Abstract

Penyakit bercak daun yang disebabkan oleh Helminthosporium oryzae merupakan salah satu penyakit yang sering menyerang tanaman padi. Terdapat strategi pengendalian dalam pertanian berkelanjutan yang dapat diterapkan, salah satunya menggunakan biopestisida ramah lingkungan yang dapat menekan perkembangan penyakit tanaman. Penggunaan biopestisida Fobio diharapkan mampu mengandung berbagai mikroorganisme, baik yang sengaja diisolasi dari perakaran tanaman maupun yang terkandung didalam medium pembawanya. Tujuan penelitan ini adalah mengetahui potensi berbagai konsentrasi biopestisida Fobio dalam menekan perkembangan patogen  H. oryzae penyebab penyakit bercak daun pada tanaman padi gogo organik. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan 4 perlakuan dan 3 ulangan. Perlakuan dalam penelitan ini adalah KT sebagai kontrol (konsentrasi 0 ml/liter), FA (konsentrasi 5 ml/liter), FB (konsentrasi 10 ml/liter), FC (konsentrasi 15 ml/liter) dengan dosis 8 liter/plot. Hasil penelitian menunjukkan bahwa formula biopestisida Fobio pada konsentrasi 10 ml/liter paling efektif dalam menghambat infeksi patogen penyakit bercak coklat yang disebabkan oleh H. oryzae dibanding perlakuan lainnya. Pada perlakuan ini intensitas serangan penyakit akibat H. oryzae adalah yang paling rendah, yaitu 53.47% pada 10 MST, menghasilkan bobot basah gabah 979.0 gram dan bobot kering gabah 887.33 gram.
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
THE ROLE OF AgNPs AND AB Mix IN THE GROWTH OF Aglaonema 'LADY VALENTINE' IN VITRO & EX VITRO Larrisa, Damasa Ines; Nugrahani, Pangesti; Wiyatiningsih, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11917

Abstract

Aglaonema ‘Lady Valentine’ is a popular ornamental plant with significant economic value. Conventional propagation methods have limitations, making tissue culture and acclimatization viable alternatives. However, low shoot multiplication and slow acclimatization remain major challenges. This study investigated the effects of silver nanoparticles (AgNPs) and AB Mix nutrient concentrations on in vitro and ex vitro plantlet growth using a two-factorial completely randomized design with four levels and three replications. The results showed varied growth responses, with the best performance observed in the 2 ppm AgNPs and 3 ml/L AB Mix treatment, which increased plantlet height, volume, and visual health. In ex vitro conditions, both 2 ppm and 3 ppm AgNPs combined with 3 ml/L AB Mix supported better plantlet adaptation, as seen in increased height, number of leaves and shoots, and seedling viability. Overall, the combination of 2 ppm AgNPs and 3 ml/L AB Mix proved most effective for enhancing multiplication and acclimatization
Bioencapsulation of Streptomyces sp. with Sodium Alginate for Control Fusarium sp. in Shallot Plants Widyananda, Kanaya Syafina; Purnawati, Arika; Wiyatiningsih, Sri
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.67882

Abstract

This study investigated the effects of Streptomyces sp. encapsulated in sodium alginate beads on the growth and health of shallot (Allium cepa L.) plants. Different sodium alginate concentrations (2%, 2.5%, and 3%) and application timings (7 days before transplanting and at transplanting) were tested. The mass and diameter of the beads showed slight decreases over storage time, indicating some shrinkage but maintaining structural integrity to protect the bacteria. Encapsulation efficiency was highest at 3% sodium alginate, which also correlated    with    increased    bead     stability.     Viability     tests     revealed that Streptomyces maintained high colony-forming units (CFU) across the treatments, confirming the protective effect of the encapsulation. Application of beads significantly reduced Fusarium wilt disease intensity and improved bulb weight. Treatments with higher sodium alginate concentrations and earlier application times showed the most pronounced benefits. These findings demonstrate that beads using optimal sodium alginate concentration and timing enhance microbial viability and biological control efficacy, contributing to healthier and more productive shallot cultivation.
Biodegradation Potential of Streptomyces sp. Against propineb-Based 70% Fungicide Under In Vitro Conditions Sari, Nadya Naurah; Mujoko, Tri; Wiyatiningsih, Sri
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.66871

Abstract

The intensive use of propineb-based fungicides in agriculture can negatively affect the environment due to toxic and persistent residues in soil. An environmentally friendly approach to reduce these impacts is bioremediation using microorganisms such as Streptomyces sp. This study aimed to examine the tolerance and biodegradation potential of Streptomyces sp. toward 70% propineb fungicide under in vitro conditions. The research was conducted at the Plant Health 1 Laboratory, Universitas Pembangunan Nasional “Veteran” East Java, from June to August 2025. The experiments were carried out in two stages: (1) a tolerance assay on Glucose Nutrient Agar containing propineb at 0; 0,50; 1,00; 1,50; and 2,00 g/L, and (2) a degradation assay using a dual-culture method with Colletotrichum sp. as a bioindicator. Observation variables included Total Plate Count (TPC) of Streptomyces sp., colony diameter of Colletotrichum sp., and the relative inhibition level (THR). Results showed that Streptomyces sp. was able to grow at all fungicide concentrations. The highest population was found in P0 (0 g/L) with 1,42×1013 CFU/mL with growth decreasing as propineb concentration increased, reaching the lowest level of 3,22×10¹⁰ CFU/mL in P4 (2.00 g/L). In the degradation assay, Colletotrichum sp. colony diameter decreased from 3,38 cm in P0 (0 g/L)  to 2,24 cm in P4 (2,00 g/L). Meanwhile, THR values increased consistently with fungicide concentration, rising from 3,17% in P0 (0 g/L)  to 35,48% in P4 (2,00 g/L).
ISOLASI, IDENTIFIKASI MORFOMETRIK, DAN POTENSI NEMATODA ENTOMOPATOGEN Heterorhabditis americana DARI LAHAN TEBU DI KEDIRI, INDONESIA SEBAGAI KANDIDAT BIOPESTISIDA RAMAH LINGKUNGAN: ISOLATION, MORPHOMETRIC IDENTIFICATION, AND POTENTIAL OF THE ENTOMOPATHOGENIC NEMATODE Heterorhabditis americana FROM SUGARCANE FIELDS IN KEDIRI, INDONESIA AS AN ENVIRONMENTALLY FRIENDLY BIOPESTICIDE CANDIDATE Simanjuntak, William Fernando; Kusuma, Ramadhani Mahendra; Wiyatiningsih, Sri
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 14 No. 1 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2026.014.1.4

Abstract

Nematoda entomopatogen (NEP) adalah organisme mikroskopis berbentuk mirip cacing yang memiliki potensi besar sebagai agens pengendali hayati. Kediri merupakan salah satu daerah penghasil tebu utama di Indonesia. Nematoda Heterorhabditis mampu membunuh uret (Lepidiota stigma) sebagai hama utama tebu, sehingga menunjukkan potensi yang besar bagi Heterorhabditis sp. sebagai musuh alami hama tanaman tebu. Penelitian ini bertujuan untuk mengeksplorasi keberadaan Heterorhabditis sp. di lahan tebu Kediri melalui pengambilan sampel tanah dan identifikasi morfometrik. Eksplorasi Heterorhabditis diawali dengan pengambilan sampel tanah pada tanaman tebu sehat kemudian dilakukan baiting NEP. Nematoda entomopatogen yang diperoleh diisolasi menggunakan metode white trap dan difiksasi sebelum diidentifikasi. Hasil penelitian menunjukkan bahwa pada tanah lahan tebu di Kediri terdapat nematoda Heterorhabditis sp. Nematoda Heterorhabditis sp. yang ditemukan memiliki panjang tubuh 467,6 µm, Lebar tubuh 26,9 µm, panjang bulbus basal 13,7 µm, dan panjang ekor 36,2 µm. Nematoda entomopatogen yang diperoleh berasal dari genus Heterorhabditis dengan dugaan spesies Heterorhabditis americana. Spesies ini berpotensi untuk dikembangkan dalam berbagai formulasi biopestisida guna mendukung pengembangan Pengelolaan Hama Terpadu (PHT) di Indonesia.
Severity Level Classification of Bacterial Leaf Blight Disease (Xanthomonas oryzae) in Rice Plants (Oryza sativa L.) Based on VARI Image Processing Rafi Dwi Nugraha; Hery Nirwanto; Sri Wiyatiningsih
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 2 (2026): April 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i2.652-662

Abstract

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae is one of the main diseases threatening rice productivity in Indonesia. This study aims to classify the severity of BLB in rice plants more effectively using drone-based image processing technology with the VARI (Visible Atmospherically Resistant Index) vegetation index approach. The study was conducted in Wonoayu Subdistrict, Sidoarjo Regency, covering an area of 17 hectares using a DJI Phantom 4 Pro drone equipped with an RGB camera for image acquisition. The data was then analysed through pre-processing, segmentation using the K-Means Clustering method and edge detection, and classification using the Convolutional Neural Network (CNN) algorithm. The results showed that the CNN classification model was able to identify five levels of attack (healthy, mild, moderate, severe, and dead) with an accuracy of 82.25%, and the classification map was able to distinguish the spatial distribution of the disease in agricultural land. This model also showed better performance than conventional monitoring methods. The use of VARI-based image processing from drones has proven effective as an early disease detection method, providing precise solutions for rice plant health management.
Co-Authors Agustin, Indah Sari Dwi Alfia Rofika Sari Arika Purnawati Armadianty, Fany Risma Boleng, Maya Stolastika Deviyanti, Nur Qomariyah Dewanti, Felicitas Deru Diasi, Ananta Rizki Didik Utomo Pribadi Dinda Rizka Rahayu Dita Megasari Dwi Afrian Dwi Afrian Dwi Suci Rahayu Dykha Maretha Setyawan Eben, Philipus Wahyu Baskoro Eko Setiyo Wawan Elvira, Nolla Dwi Endang Tri Wahyu Prasetyawati Endang Triwahyu Prasetya Endang Triwahyu Prasetyawati Ersyaf, Ihza Muhammad F. Deru Dewanti Fajri Rizky Saptono Faradila Amir Farisa Fitri Wijayanti Fitriya Pebriana Gusdino Hasyidan Gusdino Hasyidan Harijani, Wiwik Sri Haykal, Arkan Hendra Wahyudi Ariyono Hendra Wahyudi Ariyono Herry Nirwanto Hery Nirwanto Ika Nur Fitriana Indah Anita Sari Krisna Bayu Prasetiyo Laila, Nita Nur Larrisa, Damasa Ines Lia Iswindari Mukaromah Maroeto ., Maroeto Maroeto Maroeto Moch Nur Yudha Moch. Arifin Mohamad Hipti Mutiara Alyzza Bilqis Nabila Alysia Multazam Nabila Naylofar Nauval Zakqy Nia Rulinggar Putri M Pangesti Nugrahani Penta Suryaminarsih Penta SURYAMINARSIH Pratama Wirya Atmaja Pribadi, Didik Utomo Rafi Dwi Nugraha Ramadhani Mahendra Kusuma Rani, Sasiska Ratna Arifiani Sukmaningrum Retno Sulistiyowati Riko Setya Wijaya Robithotul Ummah Rosyiidah Sholihin, Alyaa Arwaa Santoso, Wahyu Sarah Hikmah Marieska Sarah Hikmah Marieska Sari, Nadya Naurah Sentoso, Wahyu Septikahady, Hani Shafa, Yola Salsabila Shavira Dwi Livita Reza Sigit Dwi Nugroho Simanjuntak, William Fernando Sisko Budianto Siti Fatimatus Syahrok Surya Ari Widya Suryandika, Fadila Suwandi Tri Mujoko Tri MUJOKO Tri Wahyu Prasetyawati, Endang Ustman Buchori Vikayanti Vikayanti Wahyu Santoso Wahyu Santoso Wahyu Santoso Wanti Mindari Widyananda, Kanaya Syafina Wijaya, Riko Setya Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Winadya, Archamiyanis Putri Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Yenny WURYANDARI