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All Journal Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Media Kedokteran Hewan

Ida Bagus Kade Suardana

Laboratorium Virologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana. JL. Sudirman, Sanglah, Denpasar, Bali, Indonesia 80234.

Author-ID : 386557

Education Medicine & Pharmacology Veterinary

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Immunological Detection of Rabies Virus in Brain Tissues of Infected Dogs by Monoclonal Antibodies Nyoman Mantik Astawa; Ida Bagus Kade Suardana; Luh Putu Agustini; Faiziah -
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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In order to establish an immunological detection of rabies virus in tissues of infected dogs, monoclonalantibodies (mAbs) against rabies virus (RV) were produced. The mAbs were produced by fusion of mielomacells with the lymphocytes of mice immunized with RV. The mAbs produced were then characterized andused for the detection of rabies virus in brain tissues of infected dogs. Six mAbs designated CC6, EG4,DG10, BB12, CA9 dan EB5 were used in this study. In Western blotting test, some mAbs reacted with 66KDa which is the glycoprotein of the virus. In immunoperoxidase, 2 mAbs (CC6 and DG10) detected RVin the brain of infected dogs. By direct immunoflourescence, flourescence isotyocyanate (FITC) labelledDG10 mAbs detected RV in fresh and formaldehyde fixed brain tissues. RV was detected in 12 infecteddogs but not in normal uninfected dogs. In this study it was confirmed that rabies virus can be detected inthe brain tissues of infected dogs by monoclonal antibodies.

Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR I Wayan Wirata; Ida Ayu Sri Chandra Dewi; I Gusti Ngurah Narendra Putra1,; Ida Bagus Oka Winaya; Ida Bagus Kade Suardana3,; Tri Komala Sari; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Classical Swine Fever (CSF) virus has been confirmed for the first time in pig in Bali. The object of thisstudy was suspected CSF cases diagnosed at the diagnostic laboratory assistantship of the Faculty ofVeterinary Medicine, Udayana University, in 2007-2008. Total number of cases was 12. Case recordsincluded the signalment of case (breed, age, body weight, and the origin of respective case), clinical signs,post-mortem lesions, and histological pictures. CSF virus was confirmed using the standardized reversetranscriptase-polymerase chain reaction (RT-PCR) for CSF from European Union. One RT-PCR productwas sequenced. CSF virus was confirmed in seven out of 12 cases (58%). The cDNA sequence wasconfirmed to be specific of CSF E2 protein coding region with 98% homology to one isolate from China thatwas available in GeneBank. Further works are recommended to elucidate the sensitivity of RT-PCR, toclarify some differential diagnose, and to find out the genetic variation of CSF virus in Bali.Key words: classical swine fever virus, Bali, RT-PCR

Seroprevalensi Virus Avian Influenza H9N2 pada Ayam Kampung (Gallus domesticus) di Pasar Beringkit, Kabupaten Badung, Bali Brigita Galilea Adu; Messy Saputri Boru Sembiring; Oktryna Hodesi Sibarani; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana; I Gusti Ayu Agung Suartini; Tjokorda Sari Nindhia
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Virus Avian Influenza (Avian Influenza Virus/AIV) subtipe H9N2 (AIV-H9N2) telah menjadi perhatian bagi kesehatan unggas. Virus ini telah dilaporkan di beberapa provinsi di Indonesia. Pasar Beringkit merupakan pasar unggas yang menerima suplai unggas dari berbagai daerah di Bali. Pasar ini menjual berbagai jenis unggas seperti: ayam, itik dan ayam kampung. Penelitian ini bertujuan untuk mengetahui seroprevalensi virus Avian Influenza subtipe H9N2 pada unggas domestik di pasar Beringkit, Kabupaten Badung, Bali. Sebanyak 187 sampel darah dikumpulkan dari tiga kali pengambilan yang berbeda. Serum diambil dari ayam broiler, ayam kampung dan itik yang belum divaksin dan diuji menggunakan Hambatan Hemaglutinasi (Haemagglutination Inhibition/HI). Serum diencerkan lima kali dengan NaCl dan dipanaskan 55oC selama 30 menit sebelum dilakukan pengujian. Hasil pemeriksaan uji HI dianalisis dengan uji statistik Non-parametrik Chi-Square. Hasil penelitian ini menunjukkan bahwa dari 187 sampel serum,43 sampel positif mengandung antibodi AIV-H9N2. Seroprevalensi AIV-H9N2 pada ayam broiler sebesar 15,9% (dari total 63), ayam kampung 35,5% (dari total 62) dan itik sebesar 17,7% (dari total 62). Hasil analisis statistika menunjukkan bahwa sampel yang diambil dari antar spesies dengan tiga kali pengambilan berbeda menunjukkan hasil tidak berbeda nyata. Penelitian ini menunjukkan bahwa unggas domestik di Bali telah terinfeksi AIV-H9N2. Biosekuriti, pengawasan pasar dan vaksinasi efektif dalam mencegah infeksi perlu ditingkatkan. Dampak ekonomi yang disebabkan AIV-H9N2 pada unggas domestik perlu dikaji lebih lanjut.

Respon Imun Itik Bali terhadap Berbagai Dosis Vaksin Avian Influenza H5N1 Ida Bagus Kade Suardana; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was carried out to investigate the immune response of Bali ducks against various doses ofAvian Influenza H5N1 vaccine. The study was carried out using a complete Random-Split in Time researchdesign as many as 40 of Bali ducks of 3 months age were kept separately in 4 groups. The ducks werevaccinated twice in two week interval with AI H5N1 vaccine of 0 (as negative control), 1/2, 1, and 2 doses.Sera were collected one day before first vaccination, then every week until three weeks after the secondvaccination. All sera were tested by hemaglutination inhibition (HI) test. The result shows that antibodylevel with double dose was significantly higher than single dose, half dose, and negative control (P<0.01).However antibody level in ducks vaccinated with single and half dose did not show any significant difference(P > 0.05).

Perbandingan Sekuens Konsensus Gen Hemaglutinin Virus Avian Influenza Subtipe H5N1 Asal Unggas di Indonesia dengan Subtipe H5N2 dan H5N9 I Gusti Ngurah Kade Mahardika; I Nyoman Suartha; Ida Bagus Kade Suardana; I Gusti Ayu Yuniati Kencana; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Consensus sequence of hemagglutinin (HA) gene of avian influenza viruses of H5N1 subtype isolatedfrom fowl in Indonesia – hereafter named as H5N1_Indonesia – is compared with that of H5N2 and H5N9viruses. Sequence information were downloaded from the public database GeneBank. The genetic distancesand nucleotide as well as its deduced amino-acid sequence alignment were analysed. At nucleotide level,genetic distances of HA between H5N1_Indonesia to H5N2 and H5N9 are 16.2% and 9.6%, respectively.At amino-acid level, the distances are 9.7% and 6.8%. The genetic distances of HA1 fragments are 19.0%(H5N1_Indonesia – H5N2) and 10.9% (H5N1_Indonesia – H5N9). At amino-acid, level the genetic distancesof HA1 are 13.1% (H5N1_Indonesia-H5N2) and 8.8% (H5N1_Indonesia – H5N9). All three subtypes havedifferent glycosilation motive and variation of amino-acid sequence of four antigenic sites. The consequenceof those facts is discussed.

The Production and Use of Monoclonal Antibodies for the Detection of Avian Influenza Antigen in the in Infected Chickens Nyoman Mantik Astawa; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A safe and appropriate diagnostic method for avian influenza virus (AIV) infection in chickens wasestablished using monoclonal antibodies (mAbs) against the virus. The virus used for the production of themonoclonal antibodies was an Indonesian AIV-H5N1 isolate. Immortal mouse myeloma cells were fusedwith the lymphocytes derived from the spleen of mice immunized with the virus. The mAbs were tested fortheir specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehydeinactivated virus and normal allantoic fluid as antigens. Twelve mAbs specific against AIV were isolatedand 8 mAbs were used for immunodetection of AIV antigen in chicken’s tissues. By indirect ELISA, themAbs were able to detect AIV antigen in allantoic fluid at the titre as low as 2-2 to 2-4 HA units per 0.1 ml.By immunoperoxidase staining AIV–antigen was detected in paraffin embedded tissues of AIV-infectedchickens. AIV antigen was not detected in chickens which were confirmed to be AIV negative. In theinfected chickens, high intensity of AIV antigen was detected in proventricle gland and small intestine.The AIV antigen with a lesser intensity was detected in lungs and spleen but hardly detected in muscle,brain and several other tissues. This study show clear evidences that mAbs produced in this study areapplicable for use in the detection of AIV antigen in infected chickens.

Perubahan Patologi Sistem Hepatobiliari Sapi Bali Terinfeksi Fasciola gigantica Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; I Made Kardena; Ida Bagus Windia Adnyana; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Liver fluke infections is an important parasitic disease that common attack cattle, buffalo and others ruminant. The disease in the tropics is caused by Fasciola gigantica trematodes. Fasciolosis found in Bali cattle besides causing high economic loss also has the opportunity infect humans. This study aims to determine the pathology changes of Bali cattle hepatobiliary system infected by F. gigantica. In January2019 were examined a hundred of liver tissue Bali cattle. Based on the sex as many as 75 bali cattle are cows and 25 are bulls. On macroscopic examination was found fifteen bali cattles in gallbladder containing of F. gigantica. In positive Fasciolosis the surface of liver look uneven with enlargement of lymph nodes. The tissue of Bali cattle liver infected with F. gigantica is cut into 1x1x1 cm and put in a pot that has been filled with 10% neutral buffered formaline. The fixed tissue then processed in a tissue processor and stained with hematoxylin-eosin (HE). Microscopic examination was found bile ducts proliferation and blocked of the lumen accompanied by cholangio cirrhosis. Found longitudinal pieces of Fasciola giganticaliver fluke surrounded by fibrous connective tissue and multifocal coagulative necrosis with fibroblast proliferation. In some location the infiltration of neutrophil cells around the bile ducts can also be seen. Congestion, bleeding, and neutrophil infiltration are also seen in areas of necrosis. In the bile duct wall bleeding, adenomatus hyperplasia, infiltration of mononuclear cells with mild intensity and foci ofcalcification are found. It can be concluded that there was a change with severe intensity accompanied by chronic inflammation in the hepatobiliary system of Bali cattle with Fasciolosis.

Protective Immune Response of Post Rabies Vaccinated Dogs in Buduk Village, Mengwi, Badung, Bali (RESPONS IMUN PROTEKTIF PADA ANJING PASCAVAKSINASI RABIES DI DESA BUDUK, MENGWI, BADUNG, BALI) Ida Bagus Kade Suardana; Ida Bagus Oka Winaya; Desak Wiga Puspita Dewi; I Wayan Sudarmayasa; Kadek Karang Agustina
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

In efforts to eradicate rabies disease, vaccinations have been carried out targeting so-called rabies carrier animal such as dog andcat. Mass rabies vaccination has been done by the government of Bali annualy, in every sub-village at vaccination post or at every household. Minimum vaccination coverage to protect the threatened dog population from rabies is 70%. To determine whether the vaccinated dogs have protective antibody against rabies, this study have been done to find out the immune response of dogs after rabies vaccination in Buduk village. Sera were collected using random sampling in each subvillage, a total of 30 serum sample were examined, and the antibody was tested using kit ELISA Rabies®. Elisa test resulted in 90% of dogs have protective antibody titers (OD>0.5 EU). It concluded that dogs in Buduk village have a good immune response against rabies vaccine.

Dog Demography And Level Of Knowledge Against Rabies In Positive And Negative Case Of Rabies Areas In Mendoyo-Jembrana, Bali, Indonesia (DEMOGRAFI ANJING DAN TINGKAT PENGETAHUAN MASYARAKAT TERHADAP RABIES DI DAERAH DENGAN KASUS RABIES POSITIF DAN NEGATIF Kadek Karang Agustina; Putu Sasmitha Devi Cahyani; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This research aims were to compare the demography of dogs and knowledge profile of dog owners against rabies in Mendoyo Sub-District, Jembarana Bali. This research used observational method, conducted from May to June 2016 in Mendoyo sub-district of Jembrana. Two Banjars were selected by the rabies case report in dogs (YehEmbangKangin village) and two other without case (MendoyoDanginTukad village). The data collected by interviewing the community and direct assessment of free-roaming dogs in the areas. A total of 200 household samples were taken, 50 respondents of each sampling area. This research resulted that the ratio of man-dog in Mendoyo was 4.5:1 and the ratio of male and female dogs was 1.8:1. As many as 64.8% of dog’s age were more than 12 month old, 28% were 4 to 12 month old and 8% were less than 4 month old. In the sampling areas, 19.4% of dogs were sterilized, which were 91.18% males and 8.82% females. Coverage of rabies vaccination in owned dogs was 82.3%. Dog’s petting method by people in Mendoyo was 69.71% free-roaming while 30.29% tied or caged. On free-roaming dog assessment, this study found 52 free-roaming dogs on the streets, 65.38% males and 34.62% females, only 30.8% of those used vaccination collar marker, Based on the body condition score, 76.9% of free-roaming dogs have good conditions. Of the total 200 households surveyed, there was a high level of knowledge about the danger of rabies and the importance of vaccination, but only a few people (<35%) received consulting about rabies, understood how to prevent rabies infection and the regulations about dog petting management. In positive area, we found there are still many people who have the habit of disposing dog. We conclude that there is needed to improve knowledge and attitude of the community about the prevention and risk factor of rabies.

Pelacakan Kasus Flu Burung pada Ayam dengan Reverse Transcriptase Polymerase Chain Reaction* (DETECTION OF AVIAN INFLUENZA IN CHICKENS BY REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION) Gusti Ayu Yuniati Kencana; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana; I Nyoman Mantik Astawa; Ni Made Krisna Dewi; Gusti Ngurah Narendra Putra
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian Influenza (AI) or Bird Flu is a fatal zoonotic disease caused by highly pathogenic avian influenza(HPAI) virus of H5N1 sub-type. The disease is still endemic in Indonesia. This study was conducted toinvestigate AI cases in chickens in Bali. Virus isolation was performed in 9 day-old embryonated chickeneggs, and then followed by serologic testing by haemaglutination (HA) and Haemaglutination Inhibition(HI) assay using standard microtiter procedure. All of the samples were further tested with reversetrancriptasepolymerase chain reaction (RT-PCR). All work has been done in the Biomedical and MolecularBiology Laboratory, Faculty of Veterinary Medicine, Udayana University, Denpasar, during the period2009-2011. A total of ten samples were examined A total of ten chicken samples consisting of 6 fieldsamples and 4 meat samples have been confirmed to be AIV H5N1. All field cases showed clinical signsand gross pathology that were typical to the infection of avian influenza. The result indicates that AI casesare still prevalent among chickens in Bali.

Co-Authors Akbar, Saiful Anak Agung Ayu Mirah Adi Anak Agung Sagung Kendran Anindya Novitasari Azizah, Hidayatul Brigita Galilea Adu Cahyani, Ni Luh Risna Desak Wiga Puspita Dewi devinda yuniastika Dewi, Desak Made Wiga Puspita Dewi, Putu Ayu Purbani Novia Faiziah - Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Narendra Putra Hanesty Jantiko, Hanesty I Gusti Agung Ayu Suartini I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra1, I Ketut Berata I Made Kardena I Made Sukada I Nengah Sudarmayasa I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Putu Sampurna I Putu Sudiarta I Wayan Sudarmayasa I Wayan Sudira I wayan Teguh Wibawan I Wayan Wirata I.A.P. Apsari Ida Ayu Pasti Apsari Ida Ayu Sri Chandra Dewi Ida Bagus Komang Ardana Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Jayanata, I Made Kadek Karang Agustina Ketut Tono Pasek Gelgel Lijung, Derfina Luh Putu Agustini Marson, Fransiska Gratia Sonita Messy Saputri Boru Sembiring Ni Made Krisna Dewi Ni Made Ritha Krisna Dewi Norawigaswari, Nengah Desy Oktryna Hodesi Sibarani Pawestri, Mega Mijil Pradnyandika, I Putu Krisna Ardhia Prasatya, I Gde Made Abdi Pratiwi, Ni Made Diah Kusuma Putu Sasmitha Devi Cahyani Sewoyo, Palagan Senopati Sri Kayati Widyastuti Suriana, Ni Komang Valerie Sylvia Kamil Tjokorda Sari Nindhia TRI KOMALA SARI Widyaningsih, Putu Oka Yulianti, Vanesya

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