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All Journal HAYATI Journal of Biosciences Bumi Lestari Biogenesis: Jurnal Ilmiah Biologi KUKILA SIMBIOSIS Metamorfosa: Journal of Biological Science Open Access DRIVERset
SANG KETUT SUDIRGA
Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Other

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Journal : simbiosis

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KUALITAS SPERMATOZOA DAN BERAT ORGAN REPRODUKSI MENCIT (Mus musculus) SETELAH PEMBERIAN EKSTRAK BIJI KLABET (Trigonella foenum-graecum L.) Maulidya Rosa Putri Utama; Dwi Ariani Yulihastuti; Sang Ketut Sudirga
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p04

Abstract

Indonesia is one of the countries known to have the highest biodiversity in the world. There are 18 types of herbs that have the potential as antifertility in men, one of which is the klabet plant or fenugreek. Klabet is believed by ordinary people to have properties to increase the production and quality of spermatozoa. According to previous research klabet has toxic effects that can inhibit fertility due to the content of phytochemicals in it. This study aims to determine the effect of fenugreek seed extract on the quality of spermatozoa and the weight of the reproductive organs of mice. The mice used were 20 male mice weighing about 25-30 grams divided into 4 treatments namely control (P0), 56 mg/kgBB (P1), 112 mg/kgBB (P2), 168 mg/kgBB (P3) with 5 repetitions. Giving treatment for 24 days. The method used is RAL (Complete Randomized Design) and the resulting data is analyzed using ANOVA. The parameters studied were testicular weight, epididymis weight, motility, morphology and number of male mouse spermatozoa. The results showed that there was a real difference (p≤0.05) between the control and treatment of the klabet seed extract on the quality of spermatozoa and the weight of the testicle but not significantly different (p≥0.05) to the weight of the epididyimis. Concentration of mice spermatozoa decreased by 10.60%, progressive spermatozoa motility decreased by 55.61%, nomal morphology decreased by 57.92%, while testicular weight decreased by 38.46%. The most effective dose of fenugreek seed extract used as an antifertility agent was a dose of 168 mg/kgBW (P3).
DIVERSITAS ODONATA DI KAWASAN EKOWISATA SUBAK SEMBUNG, DENPASAR UTARA Ni Luh Putu Nadia Febryanti; Ni Made Suartini; Sang Ketut Sudirga
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i08.

Abstract

The Odonata order comprises two suborders: dragonflies (Suborder Anisoptera) and damselflies (Suborder Zygoptera). This research aim to detect the species and level of diversity of Odonata present in the Subak Sembung Ecotourism Area, located in the northern region of Denpasar. The research was carried out in June until July 2024 using an exploratory method at three observation points in different Odonata habitats. Each habitat was explored in the morning from 08.00 to 11.00 WITA and in the afternoon from 15.00 to 17.00 WITA. Sampling was repeated three times. Odonata samples obtained from the three habitats were then identified through the observation of morphological characteristics. Enviromental factors were measured at each point and sampling, including altitude, air temperature and humidity. Based on the results of the research carried out six spesies of Odonata were found, namely Ischnura senegalensis, Agriocnemis femina, Agriocnemis pygmaea, Pseudagrion pruinosum, Orthetrum sabina and Crocothemis servillia. Odonata data from three different habitats were calculated by diversity index, which was categorized as moderate in the kale habitat with a value of 1,36, while the water henna habitat and rice habitat were categorized as low with values of 0,55 and 0,79. Keyword: Dragonflies, ecotourism, diversity, Odonata, Subak Sembung
ISOLASI DAN UJI PATOGENITAS JAMUR Colletotrichum spp. PENYEBAB PENYAKIT ANTRAKNOSA PADA BUAH TOMAT (Solanum lycopersicum L.) : ISOLATION AND PATHOGENITY TESTING OF FUNGI Colletotrichum spp. CAUSES OF ANTHRACNOSE DISEASE ON TOMATOES (Solanum lycopersicum L.) Laila Maghfirotun Ni’mah; Sang Ketut Sudirga; Anak Agung Ketut Darmadi
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p02

Abstract

Tomatoes (Solanum lycopersicum L.) is a fruit with important economic value and is a leading horticultural commodity in Indonesia. Anthracnose is a disease that can infect tomato plants. From previous research, several types of isolates of Colletotrichum spp. Infecting different hosts can cause different disease severity. The aim of this research was to identify the Colletotrichum fungus that causes anthracnose on tomatoes from several gardens in Bali to test its level of pathogenicity. Isolation of the fungus Colletotrichum spp. using the direct planting method. The pathogenicity test uses the disease severity (KP) scoring method using the formula. Identification and morphological characterization of isolates of Colletotrichum spp. using the identification book "Illustrated Genera of Imperfect Fungi". The parameters observed were colony shape, hyphae and conidia characters and disease severity percentage (KP) on tomatoes. This research used a completely randomized design (CRD) and the data were analyzed statistically using the ANOVA (Analysis of Variance) test with SPSS version 26 software. The results of the identification of macroscopic and microscopic characters from PS, BS and SW isolates showed differences in colony, hyphae and conidia characters. The pathogenicity test results of the PS, BS, and SW isolates showed that the SW isolate was the most adaptive and pathogenic isolate. Keywords: Identification, isolate, hyphae, spores, disease severity
UJI DAYA HAMBAT JAMUR Fusarium spp. MENGGUNAKAN EKSTRAK DAUN TREMBESI : JUDUL INGGRIS FUNGI INHIBITORY Test Fusarium spp. USING LEAF EXTRACT OF TTREMBESI Ni Putu Purnamaningsih; Sang Ketut Sudirga; Ni Made Susun Parwanayomi
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p09

Abstract

ABSTRACT Production of large red chilies has decreased due to wilt disease. Continuous use of synthetic fungicides will have a negative impact on the ecosystem and health. Vegetable pesticides are one of the environmentally friendly pesticides, because they are easily decomposed in nature. Trembesi (Samanea saman (Jacq.) Merr) is one of the plants that has the potential to be used as a botanical pesticide. This research aims to determine the ability of trembesi leaf extract to inhibit the growth of Fusarium spp. Diffusion well method to determine the inhibitory power of trembesi leaf extract with optimal concentration in vitro. One-way ANOVA test was used to analyze research data. If there is a significant difference (P <0.05), then proceed with the Duncan post-hoc test. The results showed that the control group and the extract treatment group had significant differences (P <0.05). The largest inhibitory zone is a concentration of 20% with an inhibitory zone diameter of 21.37 mm Kata kunci: Extract, inhibitory, antifungal, Fusarium
Co-Authors A.A.S. A. Sukmaningsih Affrina Fauziah Anak A.G.R Dalem Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Ariyanti Ni Kadek Barnadus Nggahu Lindi Djawa Candra Dewi Oktaviawati Deglory Tunmuni Dessy Sukma Wirastuti Dessy Sukma Wirastuti Dewa Ngurah Suprapta Dwi Ariani Yulihastuti Felisitas Meli Podhi Fitria Dwijayanti I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I KETUT MUKSIN I P. G. Ardhana I.G.N. Bayu Wirayudha IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara IRIANI SETYAWATI Junita Hardini Kadek Dian Lila Sawitri Kumala Kadek Mardika Kadek Wegi Kurnilia Laila Maghfirotun Ni’mah Made Ria Defiani MADE SUSUN PARWANAYONI Margareta Dacosta Maulidya Rosa Putri Utama Muhammad Inas Riandi Ngurah Intan Wiratmini Ni Desak Made Aprilia Dewi Ni LUH ARPIWI Ni Luh Putu Nadia Febryanti Ni Made Gari Ni Made Rai Suarni Ni Made Suartini Ni Made Susun Parwanayomi Ni Putu Adriani Astiti Ni Putu Purnamaningsih Ni Wayan Sudatri Nur Assiam Nyoman Darsini Retno Kawuri Risma Ikawaty Sajuni Widjaja Sang Ayu Bulan Dirga Pradnyani Shelly Burgin Silviana Febrionita Meiskia Agung Susun Susun Prawanayoni VEMY CLAUDIA Wafiatul Fitriyah Yan Ramona Yusni Khusnita Pangesti