Article Per Year (5 Year)
p-Index From 2021 - 2026
3.77
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Jurnal Primatologi Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Jurnal Bios Logos JURNAL KIMIA SAINS DAN APLIKASI Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) IPTEK Journal of Proceedings Series Jurnal Penelitian Hasil Hutan agriTECH Majalah Obat Tradisional JURNAL BIOLOGI INDONESIA Science and Technology Indonesia Al-Kimia Acta Pharmaciae Indonesia : Acta Pharm Indo Indonesian Journal of Chemistry Menara Perkebunan Jurnal Jamu Indonesia Jurnal Penelitian Hasil Hutan ZOOTEC Jurnal Kedokteran Hewan al Kimiya : Jurnal Ilmu Kimia dan Terapan Indonesian Journal of Jamu Jurnal Penelitian Hasil Hutan
Irma Herawati Suparto
Department Of Chemistry FMIPA IPB University, IPB Campus, Dramaga, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Veterinary Other

Published : 53 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search
Journal : HAYATI Journal of Biosciences

 1 
High carbohydrate as obese diet is not yet available commercially for monkeys. Therefore, this preliminary study was to carry out nutrient intake and digestibility of cynomolgus monkeys (Macaca fascicularis) fed with high soluble carbohydrate diet compared to monkey chow. Five adult female macaques (average body weight 2.67 kg) were made to consume freshly  diet. Commercial monkey chows (contains 3500 cal/g energy and 35% starch) were fed to three adult females (average body weight 3.62 kg). Nut DEWI APRI ASTUTI; IRMA HERAWATI SUPARTO; DONDIN SAJUTHI; I NENGAH BUDIARSA
HAYATI Journal of Biosciences Vol. 16 No. 4 (2009): December 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.16.4.147

Abstract

High carbohydrate as obese diet is not yet available commercially for monkeys. Therefore, this preliminary study was to carry out nutrient intake and digestibility of cynomolgus monkeys (Macaca fascicularis) fed with high soluble carbohydrate diet compared to monkey chow. Five adult female macaques (average body weight 2.67 kg) were made to consume freshly  diet. Commercial monkey chows (contains 3500 cal/g energy and 35% starch) were fed to three adult females (average body weight 3.62 kg). Nutrient intakes and digestibility parameters were measured using modified metabolic cages. Result showed that  average of protein, fat, starch, and energy intakes in treatment diet were higher than control diet (T-test). Fat intake in the treatment diet was three times higher, while starch and energy intakes were almost two times higher than monkey chow. Digestibility percentage of all nutrients were the same in both diets except for the protein. The study concludes that the freshly prepared high sugar diet was palatable and digestible for the cynomolgus monkeys. Further studies are in progress to develop obese diet high in energy content based on fat and source of starch treatments.         Key words: Macaca fascicularis, obese, metabolic cages, intake, digestibility
Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells Silmi Mariya; Fitriya Nur Annisa Dewi; Irma Herawati Suparto; Gregory K. Wilkerson; J. Mark Cline; . Permanawati; Diah Iskandriati; I Nengah Budiarsa; Dondin Sajuthi
HAYATI Journal of Biosciences Vol. 24 No. 3 (2017): July 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1422.555 KB) | DOI: 10.4308/hjb.24.3.136

Abstract

The mammary gland contains adult stem cells that are capable of self-renewal and are likely target for neoplastic transformation leading to breast cancer. In this study, we developed a cell culture derived from the mammary glands of cynomolgus monkeys (Macaca fascicularis)(MfMC) and furthermore identified the expression of markers for stemness and estrogenreceptor-associated activities. We found that the primary culture can be successfully subcultured to at least 3 passages, primarily epithelial-like in morphology, the cultured cells remained heterogenous in phenotype as they expressed epithelial cell markers CD24, CK18, and marker for fibroblast S1004A. Importantly, the cell population also consistently expressed the markers of mammary stem cells (ITGB1 or CD29 and ITGA6 or CD49f), mesenchymal stem cells (CD73 and CD105) and pluripotency (NANOG, OCT4, SOX2). In addition to this, the cells were also positive for Estrogen Receptor (ER), and ER-activated marker Trefoil Factor 1, suggesting an estrogen responsiveness of the culture model. These results indicate that our cell culture model is a reliable model for acquiring a population of cells with mammary stem cell properties and that these cultures may also serve as a reservoir from which more purified populations of stem cell populations can be isolated in the future.
Differentiation of Cardiomyocytes and Identification of Cardiac Conduction System Connexins Derived from Bone Marrow Mesenchymal Stem Cells of Macaca nemestrina Agus Harsoyo; Irma Herawati Suparto; Yoga Yuniadi; Arief Boediono; Dondin Sajuthi
HAYATI Journal of Biosciences Vol. 27 No. 4 (2020): October 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.4.337

Abstract

Bone marrow mesenchymal stem cells have been widely used, because plasticity, specific surface markers, self-renewal to transform into various lineages including cardiomyocytes. Information about the connexin (Cx) cardiac conduction systems of the pigtail macaque (Macaca nemestrina) is limited. This study aimed to evaluate cardiomyocyte differentiation from bone marrow mesenchymal stem cells of pigtail macaques and to clarify the Cx cardiac conduction system. Bone marrow aspirates were obtained from the proximal humerus of four adult male pigtail macaques, collected into heparinized tubes, then centrifuged to obtain mononuclear cells that were isolated and cultured in an incubator. After these cells reached 70–80% monolayer confluency as homogeneous fibroblast-like cells, they were subcultured. On the second subculture passage, the cells were pelleted to extract the mRNA, which was analysed by reverse transcription–polymerase chain reaction, and then cultured for a third passage. Cells were positive for CD73 and CD105 and the reference gene glyceraldehyde-3-phosphate dehydrogenase, and negative for CD34 and CD45. Osteogenic, chondrogenic, adipogenic, and cardiomyocyte differentiation was confirmed based on specific staining. The pigtail macaque bone marrow mesenchymal stem cells can be isolated and subcultured. The transcription of genes and translation of proteins of the connexin cardiac conduction systems was successfully identified.
Expression of SARS-CoV-2 Nucleocapsid (N) Recombinant Protein Using Escherichia coli System Rizki Aulia Ansari; Uus Saepuloh; Silmi Mariya; Yuliana; Rachmitasari Noviana; Irma Herawati Suparto; Huda Shalahudin Darusman
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.445-450

Abstract

One of the main antigen that can be used for serological testing is the nucleocapsid (N) which is the most abundant viral-derived protein in SARS-CoV-2 where this virus can cause COVID19 disease. The aim of this study was to develop the SARS-CoV-2 N recombinant protein using Escherichia coli expression system. A total of 1,089 nucleotides encoding 362 amino acids of SARS-CoV-2 N was cloned to pET-14b vector. The plasmid then expressed in E. coli BL21 (DE3) and induced with 1.0 mM IPTG (Isopropyl-β-d-1-thiogalactopyranoside). The cell was harvested using denaturation lysis buffer due to inclusion body formation of SARS-CoV-2 N protein. Dialysis processed and concentrated using PEG-6000 resulted 0.992 mg/ml protein yield. Analysis of SARS-CoV-2 N recombinant protein using SDS-PAGE technique showed approximately 37.0 kDa specific band target protein. Application of this SARS-CoV-2 N recombinant protein to vaccinated and non-vaccinated antibody serum samples using ELISA technique indicated the significant result of optical density mean at 0.603 and 0.135, respectively. This study revealed that the production of SARS-COV-2 N recombinant protein could be carried out in E. coli expression system under denatured conditions, therefore the methods are more effective in producing the protein as a basic material in immuno-diagnostic assay.
Expression of APP, CDK5, and AKT1 Gene Related to Alzheimer Disease in Brain of Long-tailed Macaques Lis Rosmanah; Uus Saepuloh; Sela Septima Mariya; Irma Herawati Suparto; Wasmen Manalu; Adi Winarto; Huda Shalahudin Darusman
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.145-152

Abstract

Amyloid plaques and Neurofibrillary Tangles (NFTs) are known to be key pathological features of Alzheimer disease. To gain a better understanding of this disease, studies were carried out on the Indonesian primates, the long-tailed macaques, using a spontaneous Alzheimer's disease model. Examining and identifying genetic markers involved in plaque formation and NFTs in long-tailed macaques is necessary to reveal their physiological processes. In this study, the expression of genes involved in the development of amyloid plaque (Amyloid Precursor Protein (APP)) and those that control the phosphorylation of tau protein (CDK5 and AKT1) was examined in the long-tailed macaque brain. This study showed that APP, CDK5, and AKT1 may potentially be developed as genetic markers of Alzheimer's disease. Long-tailed macaques exhibited the development of amyloid plaque in the aging brain based on the analysis of the gene expression profile of its biomarker. Furthermore, long-tailed macaques can be optimized for neurodegenerative models.
Co-Authors . NURHAIMI-HARIS . Permanawati Adi Winarto Agus Harsoyo Agus Malik Ibrahim Alfa A Perdana Alzamori, Winda Amalia Yunita Amelia Marzanti Amrina Rosyada Andi Nursanti Ani Suryani Suryani Annisa Dewi, Fitriya Nur Arief Boediono Auliatifani, Reza Auliya Ilmiawati Baiq NM Utami Bella Fitri Rahmantika Cece Sumantri Cecep Abdurohman Putra Charlena Chonny Damayanti Chusnul Choliq Chusnul Choliq Daniel Putra Oktavianus Laia Dede R. Adawiyah Dedy Duryadi Solihin DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa Dewi, Tri Isyani Tungga Diah Iskandriati Dondin Sajuthi Dudi Tohir Dwi Ramadhani Ekowati Handharyani Era Rahmi Eti Rohaeti Ferian A Setiyoko Fiqa Annisa Rakhmatika Fitriah Sari Fransiska Rungkat Zakaria Gina, Safira Gregory K. Wilkerson Gustan Pari Gustan Pari Harrold Halilintar MR Subu Taopan Huda Shalahudin Darusman I Nengah Budiarsa I NENGAH BUDIARSA Ibrahim, Agus Malik Ida Ayu Putu Sri Widnyani Iin Indriawati Iin Kurnia Hasan Basri Irmanida Batubara J. Mark Cline Khaydanur Khaydanur Khaydanur, Khaydanur Laela Wulansari Latifah Kosim Darusman Lilis Nurhadijah Lis Rosmanah Luthfiana, Widya M. Rafi Manasye Erlangga Mohamad Rafi Mohamad Rafi Mohamad Rafi Munawaroh, Fauziyatul Nengsih, Nunuk Kurniati Noviyan Darmawan Nunuk Kurniati Nengsih Nur Inayatullah Nurhadijah, Lilis Nurul Siti AYUNINGTIAS PAMUNGKAS, DIAH DARU Perdana, Alfa A Permanawati Permanawati PERMANAWATI, PERMANAWATI Pijoh, Deyv Purnomo, Tsania Firqin Rachmitasari Noviana Rachmitasari Noviana Rahmagiarti, Cintera Rakhmatika, Fiqa Annisa Reno Fitri Hasrini Retno Wulansari Reza Auliatifani Ria Oktarina Riska Widya Aprianti Rizki Aulia Ansari Rudi Heryanto SATRIYAS ILYAS Sela Septima Mariya Septaningsih, Dewi Anggraini Septi Iriani Setiyoko, Ferian A Silmi Mariya Silmi Mariya Silmi Mariya Silmi Mariya Silmi Mariya SILMI MARIYA Sri Rahmatul Laila Sri Supraptini Mansjoer Subu Taopan, Harrold Halilintar MR Sulistiyani Sulistiyani, Sulistiyani SUMINAR SETIATI ACHMADI Teja Kisnanto Tetty Kemala Tri Isyani Tungga Dewi Tsania Firqin Purnomo Ummah, Isna Mustafiatul Utami, Baiq NM Uus Saepuloh Uus Saepuloh Veby Tri Yani Veby Tri Yani Wasmen Manalu Widyawati Widyawati Widyawati Widyawati Wildayati, Wildayati Wina Yulianti Wisaeni Intannia Wisnu Ananta Kusuma Wulan Tri Wahyuni Wulansari, Laela Yessie Widya Sari Yoga Yuniadi Yuliana Yuni Cahya Endrawati Yusuf Ryadi Zuraida Zuraida Zuraida