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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Jurnal Primatologi Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Jurnal Bios Logos JURNAL KIMIA SAINS DAN APLIKASI Jurnal Sains dan Teknologi Nuklir Indonesia (Indonesian Journal of Nuclear Science and Technology) IPTEK Journal of Proceedings Series Jurnal Penelitian Hasil Hutan agriTECH Majalah Obat Tradisional JURNAL BIOLOGI INDONESIA Science and Technology Indonesia Al-Kimia Acta Pharmaciae Indonesia : Acta Pharm Indo Indonesian Journal of Chemistry Menara Perkebunan Jurnal Jamu Indonesia Jurnal Penelitian Hasil Hutan ZOOTEC Jurnal Kedokteran Hewan al Kimiya : Jurnal Ilmu Kimia dan Terapan Indonesian Journal of Jamu Jurnal Penelitian Hasil Hutan
Irma Herawati Suparto
Department Of Chemistry FMIPA IPB University, IPB Campus, Dramaga, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Veterinary Other

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Journal : Jurnal Kedokteran Hewan

 1 
CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM WHITE ADIPOSE TISSUE OF Macaca fascicularis Harrold Halilintar MR Subu Taopan; Fitriya Nur Annisa Dewi; Silmi Mariya; Huda Darusman; Permanawati Permanawati; Dondin Sajuthi; Irma Herawati Suparto
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.996 KB) | DOI: 10.21157/j.ked.hewan.v15i4.18203

Abstract

 Mesenchymal stem cells (MSC) derived from white adipose tissue are considered to be an appropriate therapeutic candidate for various human diseases due to their natural ability to maintain homeostasis. They have also been considered for use as a therapeutic alternative due to their sufficient availability and ease of harvesting. MSC play an important role in the process of adipogenesis and energy metabolism that is directly related to the pathogenesis of various degenerative diseases and metabolic syndromes. The purpose of this study was to obtain MSC cultures from white adipose tissue biopsied from three adult males Macaca fascicularis and to evaluate these cultures qualitatively and quantitatively regarding their characteristics and ability to differentiate. Polymerase chain reaction (PCR) was used to confirm the expression of mesenchymal stem cell-specific gene markers, and a specific growth medium was used to differentiate these cells into adipocytes, chondrocytes, and osteocytes. The results obtained showed that the MSC culture from M. fascicularis WAT had a fibroblast-like morphology with a spindle shape. The MSC markers of CD73, CD90 and CD105, were also expressed. The MSC culture was also able to differentiate into adipocytes, chondrocytes, and osteocytes. Based on these results, white adipose tissue from Macaca fascicularis was found to have potential as a source of MSC.
CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM WHITE ADIPOSE TISSUE OF Macaca fascicularis Subu Taopan, Harrold Halilintar MR; Annisa Dewi, Fitriya Nur; Mariya, Silmi; Darusman, Huda; Permanawati, Permanawati; Sajuthi, Dondin; Suparto, Irma Herawati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.18203

Abstract

Mesenchymal stem cells (MSC) derived from white adipose tissue are considered to be an appropriate therapeutic candidate for various human diseases due to their natural ability to maintain homeostasis. They have also been considered for use as a therapeutic alternative due to their sufficient availability and ease of harvesting. MSC play an important role in the process of adipogenesis and energy metabolism that is directly related to the pathogenesis of various degenerative diseases and metabolic syndromes. The purpose of this study was to obtain MSC cultures from white adipose tissue biopsied from three adult males Macaca fascicularis and to evaluate these cultures qualitatively and quantitatively regarding their characteristics and ability to differentiate. Polymerase chain reaction (PCR) was used to confirm the expression of mesenchymal stem cell-specific gene markers, and a specific growth medium was used to differentiate these cells into adipocytes, chondrocytes, and osteocytes. The results obtained showed that the MSC culture from M. fascicularis WAT had a fibroblast-like morphology with a spindle shape. The MSC markers of CD73, CD90 and CD105, were also expressed. The MSC culture was also able to differentiate into adipocytes, chondrocytes, and osteocytes. Based on these results, white adipose tissue from Macaca fascicularis was found to have potential as a source of MSC.
Co-Authors . NURHAIMI-HARIS . Permanawati Adi Winarto Agus Harsoyo Agus Malik Ibrahim Alfa A Perdana Alzamori, Winda Amalia Yunita Amelia Marzanti Amrina Rosyada Andi Nursanti Ani Suryani Suryani Annisa Dewi, Fitriya Nur Arief Boediono Auliatifani, Reza Auliya Ilmiawati Baiq NM Utami Bella Fitri Rahmantika Cece Sumantri Cecep Abdurohman Putra Charlena Chonny Damayanti Chusnul Choliq Chusnul Choliq Daniel Putra Oktavianus Laia Dede R. Adawiyah Dedy Duryadi Solihin DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa Dewi, Tri Isyani Tungga Diah Iskandriati Dondin Sajuthi Dudi Tohir Dwi Ramadhani Ekowati Handharyani Era Rahmi Eti Rohaeti Ferian A Setiyoko Fiqa Annisa Rakhmatika Fitriah Sari Fransiska Rungkat Zakaria Gina, Safira Gregory K. Wilkerson Gustan Pari Gustan Pari Harrold Halilintar MR Subu Taopan Huda Shalahudin Darusman I Nengah Budiarsa I NENGAH BUDIARSA Ibrahim, Agus Malik Ida Ayu Putu Sri Widnyani Iin Indriawati Iin Kurnia Hasan Basri Irmanida Batubara J. Mark Cline Khaydanur Khaydanur Khaydanur, Khaydanur Laela Wulansari Latifah Kosim Darusman Lilis Nurhadijah Lis Rosmanah Luthfiana, Widya M. Rafi Manasye Erlangga Mohamad Rafi Mohamad Rafi Mohamad Rafi Munawaroh, Fauziyatul Nengsih, Nunuk Kurniati Noviyan Darmawan Nunuk Kurniati Nengsih Nur Inayatullah Nurhadijah, Lilis Nurul Siti AYUNINGTIAS PAMUNGKAS, DIAH DARU Perdana, Alfa A Permanawati Permanawati PERMANAWATI, PERMANAWATI Pijoh, Deyv Purnomo, Tsania Firqin Rachmitasari Noviana Rachmitasari Noviana Rahmagiarti, Cintera Rakhmatika, Fiqa Annisa Reno Fitri Hasrini Retno Wulansari Reza Auliatifani Ria Oktarina Riska Widya Aprianti Rizki Aulia Ansari Rudi Heryanto SATRIYAS ILYAS Sela Septima Mariya Septaningsih, Dewi Anggraini Septi Iriani Setiyoko, Ferian A Silmi Mariya Silmi Mariya SILMI MARIYA Silmi Mariya Silmi Mariya Silmi Mariya Sri Rahmatul Laila Sri Supraptini Mansjoer Subu Taopan, Harrold Halilintar MR Sulistiyani Sulistiyani, Sulistiyani SUMINAR SETIATI ACHMADI Teja Kisnanto Tetty Kemala Tri Isyani Tungga Dewi Tsania Firqin Purnomo Ummah, Isna Mustafiatul Utami, Baiq NM Uus Saepuloh Uus Saepuloh Veby Tri Yani Veby Tri Yani Wasmen Manalu Widyawati Widyawati Widyawati Widyawati Wildayati, Wildayati Wina Yulianti Wisaeni Intannia Wisnu Ananta Kusuma Wulan Tri Wahyuni Wulansari, Laela Yessie Widya Sari Yoga Yuniadi Yuliana Yuni Cahya Endrawati Yusuf Ryadi Zuraida Zuraida Zuraida