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Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Rafi, Mohamad; Wulansari, Laela; Septaningsih, Dewi Anggraini; Purnomo, Tsania Firqin; Auliatifani, Reza; Khaydanur, Khaydanur; Ilmiawati, Auliya; Yulianti, Wina; Nengsih, Nunuk Kurniati; Suparto, Irma Herawati; Kusuma, Wisnu Ananta
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.
Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Mohamad Rafi; Laela Wulansari; Dewi Anggraini Septaningsih; Tsania Firqin Purnomo; Reza Auliatifani; Khaydanur Khaydanur; Auliya Ilmiawati; Wina Yulianti; Nunuk Kurniati Nengsih; Irma Herawati Suparto; Wisnu Ananta Kusuma
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.14

Abstract

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.
Co-Authors . NURHAIMI-HARIS . Permanawati Adi Winarto Agus Harsoyo Alfa A Perdana Alzamori, Winda Amalia Yunita Amelia Marzanti Amrina Rosyada Andi Nursanti Ani Suryani Suryani Arief Boediono Auliatifani, Reza Auliya Ilmiawati Baiq NM Utami Bella Fitri Rahmantika Cece Sumantri Cecep Abdurohman Putra Charlena Chonny Damayanti Chusnul Choliq Chusnul Choliq Daniel Putra Oktavianus Laia Dede R. Adawiyah Dedy Duryadi Solihin DEWI APRI ASTUTI Dewi, Fitriya Nur Annisa Diah Iskandriati Dondin Sajuthi Dudi Tohir Dwi Ramadhani Ekowati Handharyani Era Rahmi Eti Rohaeti Fauziyatul Munawaroh Ferian A Setiyoko Fiqa Annisa Rakhmatika Fitriah Sari Fransiska Rungkat Zakaria Gina, Safira Gregory K. Wilkerson Gustan Pari Harrold Halilintar MR Subu Taopan Huda Shalahudin Darusman I Nengah Budiarsa I NENGAH BUDIARSA Ibrahim, Agus Malik Ida Ayu Putu Sri Widnyani Iin Indriawati Iin Kurnia Hasan Basri Irmanida Batubara J. Mark Cline Khaydanur Khaydanur Khaydanur, Khaydanur Laela Wulansari Latifah Kosim Darusman Lilis Nurhadijah Lis Rosmanah Luthfiana, Widya M. Rafi Manasye Erlangga Mohamad Rafi Mohamad Rafi Mohamad Rafi Munawaroh, Fauziyatul Nengsih, Nunuk Kurniati Noviyan Darmawan Nunuk Kurniati Nengsih Nur Inayatullah Nurul Siti AYUNINGTIAS PAMUNGKAS, DIAH DARU Permanawati Permanawati Pijoh, Deyv Purnomo, Tsania Firqin Rachmitasari Noviana Rachmitasari Noviana Rahmagiarti, Cintera Reno Fitri Hasrini Retno Wulansari Reza Auliatifani Ria Oktarina Riska Widya Aprianti Rizki Aulia Ansari Rudi Heryanto SATRIYAS ILYAS Sela Septima Mariya Septaningsih, Dewi Anggraini Septi Iriani Silmi Mariya Silmi Mariya Silmi Mariya Silmi Mariya Silmi Mariya Sri Rahmatul Laila Sri Supraptini Mansjoer Sulistiyani, Sulistiyani SUMINAR SETIATI ACHMADI Teja Kisnanto Tetty Kemala Tri Isyani Tungga Dewi Tsania Firqin Purnomo Ummah, Isna Mustafiatul Uus Saepuloh Uus Saepuloh Veby Tri Yani Wasmen Manalu Widya Luthfiana Widyawati Widyawati Wildayati, Wildayati Wina Yulianti Winda Alzamori Wisaeni Intannia Wisnu Ananta Kusuma Wulan Tri Wahyuni Wulansari, Laela Yessie Widya Sari Yoga Yuniadi Yuliana Yuni Cahya Endrawati Yusuf Ryadi Zuraida Zuraida