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All Journal Jurnal Gizi dan Pangan Marine Fisheries: Jurnal Teknologi dan Manajemen Perikanan Laut Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis JURNAL GIZI INDONESIA Jurnal Akuatika Jurnal Ilmu dan Teknologi Pangan JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Akuatik: Jurnal Sumberdaya Perairan Dinamika Maritim Sains Natural: Journal of Biology and Chemistry Indonesian Fisheries Research Journal Jurnal Penelitian Perikanan Indonesia Journal of Marine and Coastal Science
Pipih Suptijah
Departeman Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor, Jawa Barat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Other

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ISOLASI DAN KARAKTERISASI KOLAGEN DARI KULIT IKAN PATIN (Pangasius sp.) Pipih Suptijah; Dini Indriani; Supriyono Eko Wardoyo
JURNAL SAINS NATURAL Vol. 8 No. 1 (2018): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.85 KB) | DOI: 10.31938/jsn.v8i1.106

Abstract

Isolation and Characterization of Collagen from the Skin of Catfish (Pangasius sp.)           Skin of catfish is one of aquatic by-products which could be used as an alternative source of collagen. This research is aimed to isolate and characterize collagen from skin of catfish. Methods of  isolation of collagen included three stages, the first was deproteinization using NaOH solution with concentration of 0.05 M; 0.10 M; 0.15 M; 0.20 M for 12 hours, the second was soaking in CH3COOH solution with concentration of 0.05 M; 0.10 M; 0.15 M; and 0.20 M for 2 hours, and the third was extraction in water at a temperature of 40 0C for 2 hours; characterization of collagen was included chemical and physical properties. The results showed that the best extraction method ofcollagen from skin of catfish was soaking the skin in 0.05 M NaOH solution for 12 hours and soaking the skin in 0.05 M acetic acid for 2 hours. Extraction yields of collagen was 12.15%. Chemical characteristics included proximate and amino acid composition. Proximate value of collagen consisted of moisture was 6.55%, ash 1.80%,  protein 64.74% and fat 8.85%.  The major amino acid composition of collagen were glycine, proline, alanine, arginine and glutamate. Physical characteristics of collagen resulted from FTIR analysis showed amide A, amide B, amide I, amide II and amide III, triple helical structure of the amide I and amide III indicates that the compound produced was collagen; color analysis was 66.39%; thermal analysis showed a melting temperature peak was 154.47 0C and pH value was 5.34.Keywords : Catfish, isolation, characterization, collagen, skin ABSTRAK          Kulit ikan patin merupakan salah satu limbah hasil perairan yang dapat digunakan sebagai sumber alternatif kolagen. Penelitian ini bertujuan untuk mengisolasi dan karakterisasi kolagen yang diperoleh dari kulit ikan patin. Isolasi kolagen yang dilakukan meliputi tiga tahap, yaitu tahap pertama adalah proses deproteinisasi menggunakan larutan NaOH dengan konsentrasi, yaitu 0,05 M; 0,10 M; 0,15 M; 0,20 M dan lama waktu perendaman selama 12 jam; tahap kedua, yaitu perendaman dalam larutan CH3COOH dengan empat konsentrasi CH3COOH yaitu 0,05 M; 0,10 M; 0,15 M; dan 0,20 M dan lama waktu perendaman selama 2 jam; dan tahap ketiga, yaitu ekstraksi dengan air pada suhu 40 0C selama 2 jam; serta karakterisasi kolagen yang dilakukan, meliputi sifat kimia dan fisik. Hasil penelitian menunjukkan bahwa metode ekstraksi kolagen dari kulit ikan patin  terbaik diperoleh melalui proses perendaman kulit dalam larutan NaOH 0,05 M selama 12 jam dan  perendaman kulit dalam asam asetat 0,05 M selama 2 jam.  Rendemen serbuk kolagen yang dihasilkan sebesar 12,15 %. Karakteristik kimia meliputi proksimat dan komposisi asam amino. Nilai proksimat kolagen terdiri dari kadar air 6,55 %,  abu 1,80 %, protein 64,74 % dan lemak 8,85 %. Komposisi asam amino yang dominan pada kolagen adalah glisina, prolina, alanina, arginina dan glutamat. Karakteristik fisik kolagen yang dihasilkan adalah analisis FTIR menunjukkan adanya gugus amida A, amida B, amida I, amida II dan amida III, struktur triple heliks pada amida I dan amida III mengindikasikan bahwa senyawa yang dihasilkan adalah kolagen; analisis warna  yaitu 66,39 %; analisis termal yang menunjukkan suhu puncak pelelehan adalah 154,47 0C dan nilai pH kolagen yaitu 5,34. Kata kunci : Ikan patin, isolasi, karakterisasi, kolagen, kulit 
Antimicrobial Activity of Nanochitosan Shrimp Shell as a Hands Sanitizer Safrina Dyah Hardiningtyas; Dona Fradilla Bahri; Pipih Suptijah
Journal of Marine and Coastal Science Vol. 11 No. 1 (2022): FEBRUARY
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v11i1.33821

Abstract

Chitosan is a biopolymer that can be extracted from crustaceans, such as shrimp shells. Chitosan is widely used in various fields, such as pharmaceuticals, food, and cosmetics because it is biodegradable, non-toxic, and has antibacterial activity. Nanochitosan is a dosage form based on nanotechnology which has more reactive properties. The purpose of this study was to characterize chitosan and nanochitosan and evaluate the effectiveness of nanochitosan as an antibacterial in hand sanitizer. The tests carried out included analysis of the functional group of chitosan with FTIR, particle size analysis with particle size analyzer, morphological analysis of nanochitosan with SEM, and analysis of the effectiveness of hand sanitizers. The test chitosan used had a degree of deacetylation of 85.94%. The particle size distribution of nanochitosan was 140.94 nm with a polydispersion intensity (PDI) of 0.5830. The results of the antibacterial effectiveness test of nanochitosan as a hand sanitizer showed that the higher the concentration of nanochitosan used, the total bacterial colonies would decrease. The concentration of 2000 ppm nanochitosan can reduce 93.4% of bacterial colonies on probandus hands, and this activity is known to be not significantly different from commercial hand sanitizers.
EFFECTS OF NEUTRALIZATION PERIOD AND TEMPERATURE IN ALKALI REFINING ON THE QUALITY OF SARDINE OIL Hari Eko lrianto; Sri Ningrum Pudjiastuti; Pipih Suptijah
Indonesian Fisheries Research Journal Vol 1, No 1 (1995): (1995)
Publisher : Research Center for Fisheries

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5929.192 KB) | DOI: 10.15578/ifrj.1.1.1995.87-99

Abstract

Most fish meal weste oil encountered in Indonesia has high free fatty acid (FFA)value, dark colour end strong fishy odour. Thus, the oil needs refining process for thequality improvement before-further uses, such as by alkali refining application.
PENGARUH KADAR TEPUNG KEPALA UDANG DAN WAKTU INKUBASI TERHADAP PRODUKSI BIOMASSA PROTEIN SEL TUNGGAL Mohammad saleh; Marina Marina; Endang sri Heruwati; pipih suptijah
Jurnal Penelitian Perikanan Indonesia Vol 4, No 1 (1998): (Vol.4 No.1 1998)
Publisher : Pusat Riset Perikanan, BRSDM KP.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4798.021 KB) | DOI: 10.15578/jppi.4.1.1998.88-95

Abstract

Percobaan pemanfaatan tepung kepala udang untuk pembuatan protein sel tunggal telah dilakukan untuk mendapatkan kadar substrat dan waktu inkubasi optimum bagiproduksi maksimal biomassa sel. Tepung kepala udang dengan kadar 1%, 4%,7%, dan 10% digunakan sebagai substrat dari jamur Triehoderma uiride denganinkubasi selama 4, 6, dan 8 hari lada pH 4,5 dan suhu 28' C.
TEKNIK PERANAN KITOSAN DALAM PENINGKATAN PERTUMBUHAN TOMAT (Lycopersicum esculentum) SELAMA FASE VEGETATIF PIPIH SUPTIJAH; AGOES M. JACOB; SUGARA MURSID
Akuatik: Jurnal Sumberdaya Perairan Vol 4 No 1 (2010): AKUATIK : Jurnal Sumberdaya Perairan
Publisher : Department of Aquatic Resources Management, Faculty of Agriculture, Fisheries, and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.733 KB)

Abstract

Chitosan is product of shrimp shell waste utilization that derived from chitin. Based on its nature and characteristics, it is known that chitosan has important role in agriculture as a stimulant for the plant defense system as well as growth and production of some horticultural crops such as tomatoes. The purpose of this research is to study the influence of the chitosan treatment on the growth of tomatoes and to determine the effect of treatment combination on the growth of tomatoes. Chitosan treatment experiments on the growth of tomatoes during vegetative phase is done by Complete Random Design with 1 factor, that is chitosan treatments combination including P1 (control), P2 (chitosan soaking 25 ppm), P3 (watering and chitosan soaking 25 ppm), P4 (soaking and spraying chitosan 25 ppm) and P5 (soaking, watering and spraying chitosan 25 ppm). Chitosan soaking treatment at 25 ppm in tomato seeds have more value in growth rate and growth effort than the untreated plant. The best treatment is founded in the combination of soaking treatment with chitosan spraying in 25 ppm (P4), it’s better if it’s compared with other treatment (each parameter include the height of plants, the number of branches, the number of leaves, the length of leaves and wet weight and dry weight of the plants)
KITOSAN SEBAGAI ABSORBEN IMPURITY DALAM PEMBUATAN AGAR MEDIA PIPIH SUPTIJAH; LINAWATI HARDJITO; JOHN HALUAN; MAGGY T WIJAYA
Akuatik: Jurnal Sumberdaya Perairan Vol 4 No 2 (2010): AKUATIK : Jurnal Sumberdaya Perairan
Publisher : Department of Aquatic Resources Management, Faculty of Agriculture, Fisheries, and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.71 KB)

Abstract

Existing problem in production process for fulfilling International standard still taking as a problem for Indonesian product, therefore need modification of production process which can match with the commercial standard even also International standard. One of alternative of the production process that is through input of chitosan as impurity absorbent in its extraction, so can get high quality product of jelly for medium, and others. This research aim to application of chitosan as absorbent, to produce jelly with simple method and without chemicals, and characterize of jelly and also compare it to commercial bakto ( difco). Method used in this research is using impurity absorbent by chitosan in extraction process through chitosan filtration which obtainable absorbs can directly acquire jelly extract in order to clear of pollutant omit continued with draining. Result of jelly extract analysis showed characteristic quality of jelly produced which is viscosities 9,1cPs - 12,5cPs ( bakto 17,5cPs), value of TPC 1,8x101 CFU ( bakto 1,1x102 CFU), gel strength 261,26 gF - 297,8 gF with irrigate rate 20,9% - 23% higher than bakto jelly 16,9%. Bacterium growth through TPC test at chitosan treatment 0,1% obtained TPC value is 1,8x101 colony, while TPC value in bakto jelly is 1,1x102 colony. Sulphate value is very determine and difficult enough to decrease it. Sulphate value of jelly of chitosan absorbs treatment showed vary value, from 0,15% until 0,28%, but statistically do not differ reality. Sulphate value is lower than jelly without treatment ( 0,65%) and also is lower than bakto jelly as standard (0,35%)
PEMANFAATAN PUPUK LIMBAH AGAR-AGAR TERHADAP PERTUMBUHAN SEMAI MAHONI (Swietenia macrophylla, King) DI MEDIA TAILING TAMBANG EMAS PT ANTAM UBPE PONGKOR PIPIH SUPTIJAH; BASUKI WASIS; AYU BABY MUTIARA MANDELLA
Akuatik: Jurnal Sumberdaya Perairan Vol 5 No 1 (2011): AKUATIK : Jurnal Sumberdaya Perairan
Publisher : Department of Aquatic Resources Management, Faculty of Agriculture, Fisheries, and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.3 KB)

Abstract

The processing of agar produces 65-70% residu from all raw materials that are used. The residu in form of drugs or solid waste contain macro and micro nutrients which unfortunately have not been used optimally. The waste has a potential to use as an organic fertilizer. The effect of agar waste on growth of mahogany seed and physical and chemical properties of tailing were studied. Compost fertilizer was used as comparison to see the effectiveness of addition agar waste on growth of mahogany. The growth of mahogany seed was observed from height and diameter parameter, while the minerals content were measured by Atomic Absorption Spectrophotometry (AAS). The result shown thar the highest growth was achieved when mahogany was treated with 1% agar waste, while the biggest diameter was achieved with 1,5% agar waste. Agar waste treatment improved chemical properties of tailing but has not significant change on physical properties.
Karakteristik pepton dari limbah jeroan ikan sidat (Anguilla bicolor) sebagai nutrien untuk pertumbuhan bakteri: Peptone characteristics from eel (Anguilla bicolor) viscera as a nutrient for bacterial growth Tati Nurhayati; Raden Hilman Wirayudha; Pipih Suptijah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 1 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i1.43326

Abstract

Limbah jeroan ikan sidat (Anguilla bicolor) dari hasil samping produksi umumnya tidak dimanfaatkan dengan baik. Pemanfaatan limbah jeroan ikan sidat perlu dilakukan karena proteinnya cukup tinggi, sehingga memiliki potensi untuk dimanfaatkan menjadi salah satu produk yang memiliki nilai ekonomis tinggi yaitu pepton ikan. Penelitian ini bertujuan untuk menentukan kondisi hidrolisis optimum hidrolisat jeroan ikan sidat menggunakan enzim papain, menentukan karakteristik kimia pepton jeroan ikan sidat serta mengaplikasikan pepton jeroan ikan sidat sebagai medium pertumbuhan bakteri untuk dibandingkan dengan pepton komersial. Pepton dibuat dengan proses hidrolisis menggunakan enzim papain dengan waktu 5 jam dan suhu 60oC. Konsentrasi optimum didapat pada penggunaan enzim papain 1.000 U/mg/g. Pepton jeroan ikan sidat mengandung protein 82,1 % dan lemak 0,93%. Pepton jeroan ikan sidat memiliki kelarutan 99,9%; total nitrogen 13,12%; kadar garam 0,15%; dan pH 6. Pepton jeroan ikan sidat dapat digunakan sebagai nutrien pada media pertumbuhan bakteri Escherichia coli dengan nilai optical density (OD) yang lebih tinggi jika dibandingkan dengan pepton komersial bactopeptone, namun lebih rendah nilai pertumbuhannya jika digunakan pada media pertumbuhan untuk bakteri Staphylococcus aureus.
Pre-treatment of Lobster Shell Using Hydrochloric Acid for Nanochitosan Production Safrina, Dyah; Ulfa Safitri, Anisa; Suptijah, Pipih; Fariz Pari, Rizfi
Journal of Marine and Coastal Science Vol. 12 No. 2 (2023): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i2.45883

Abstract

Lobster shells are a by-product that can be used as a raw material for making chitin and chitosan. However, lobster shells contain a high amount of minerals that cover the chitin polymer on the shell. This chitin can be converted into chitosan by deacetylation process. Efforts that can be made to increase the efficiency of the chitosan extraction process from lobster shells are by pre-treatment using hydrochloric acid. The purpose of this study was to determine the pre-treatment time of lobster shells with hydrochloric acid and to produce nanochitosan from lobster shells. The results of this study indicate that the pretreatment time has a significant effect on the yield value of chitosan. The highest yield of chitosan was produced from the acid pre-treatment with a soaking time of 120 hours with a value of 15,6%. The quality of lobster chitosan from the best treatment has met the quality requirements based on SNI. Nanochitosan was successfully synthesized by ionic gelation method with a particle size of 357,76 nm and spherical shape based on SEM photos. Lobster nanochitosan has the potential to be applied in various fields, such as food, agriculture,and biomedical.
Penggunaan kitosan sebagai pengisi dalam pembuatan sabun transparan : The utilization of chitosan as a filler for transparency soap Ibrahim, Bustami; Suptijah, Pipih; Amin, Hijrah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 13 No. 1 (2010): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v13i1.3140

Abstract

Chitosan is a natural material extracted from deacetylated chitin of crustacean shell used for many kinds of functions, not only for food but also non food product. The research objective is to find out the effect of chitosan used for filler within transparency soap to the characteristic of physical, chemical and bioeffect of end product, and to find out which concentrate of chitosan will be used for the best product. The research was divided by two steps, pre-research and main research. Pre-research resulted that transparency soap with 5% chitosan was the best choice based on organoleptic test which was the best in toughness, and based on chemical also microbiological analyses. The soap with 5% chitosan has TPC (Total Plate Count) amount to 2.0x101, while the one without chitosan contained TPC 6.5x101. The main research showed that transparency soap with 5% chitosan has better quality compare to commercialized one.
Co-Authors . Nurjanah . Prantommy . Uju A. Alhana Abdurrahman Ali Aditya Bayu Hanura Aditya Yudha Prawira Sukarno AGOES M. JACOB Agoes M. Jacoeb Agoes Mardiono Jacoeb Ahmad Zahid Anggraeny, Dyta Anggun Ratnawulan Anjarsari Anjarsari Asadatun Abdullah AYU BABY MUTIARA MANDELLA Ayu Fitri Izaki Bagus Sukma Agung Bambang Murdiyanto Basuki Wasis Bustami Ibrahim Bustami Ibrahim cholil - anwar Dadi Rochnadi Sukarsa Darma Sri Wahyuni Dery Firdaus Desie Rachmania DEWI APRI ASTUTI Dini Indriani Dini Nurfianti Diniah Diniah Dona Fradilla Bahri Endang Sri Heruwati Erliza Noor Evy Damayanthi Fajar Kurnia Budiman Fariz Pari, Rizfi Febri Gunawan Gregoria S. S. Djarkasi Hari Eko lrianto Henggu, Krisman Umbu Heni Sri Septiani Hijrah Amin Hilda Lu’lu’in Nanda Alfira Devi Inneke F. M Rumengan Irman Febrian JOHN HALUAN Joko Santoso Kamini Kamini Kiagus Dahlan Kurniawati Kurniawati Kustiariyah Tarman Laode Muhamad Hazairin Nadia Leily Amalia Lila Rani LINAWATI HARDJITO Lucia Cecilia Mandey Luh Putu Ratna Sundari Luntungan, Aldian H. MAGGY T WIJAYA MAGGY T. SUHARTONO Mala Nurilmala Maria Ivan Budju Niga Marina Marina Mega Safithri Meydia Meydia Mohammad Saleh Mulyono S. Baskoro N. Nurjanah Nani Deviyanti Nani Nur’aenah Neni Widowati Nisa Nantami Nofri Sandria Nugroho, Teguh Setyo ovintya - yanuarizki Putri Septembriani Putri Wening Ratrinia Raden Hilman Wirayudha Rezki Kamila Rimbawan , Ruddy Suwandi safrina dyah hardiningtyas Safrina, Dyah Saptari Joan Tatra Slamet Hermanto Sri Ningrum Pudjiastuti Stenly Wullur sudianto SUGARA MURSID Sugeng Heri Suseno Supriyono Eko Wardoyo SYAHRIAL SYAHRIAL syeila - rosmalawati Talumepa, Anggun C. N. TATI NURHAYATI Taufik - Hidayat Taufik Hidayat Tetty Kemala Tika Ayu Budiarti Ulfa Safitri, Anisa Untung Trimo Laksono Widyana Ayu Kristantina Winarti Zahiruddin Wini - Trilaksani Wiyan Viyata Prinaldi Wulandari Wulandari Yayandi Gushagia Yogaswara, Ghema Yugha Subagja Yunisha Aktinidia Zahidah mawardi Zhalindri Noor Adjani