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All Journal Jurnal Veteriner Jurnal Ilmu dan Teknologi Hasil Ternak Buletin Plasma Nutfah Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika AGRIVITA, Journal of Agricultural Science JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Animal Production : Indonesian Journal of Animal Production Jurnal Ternak Jurnal Agriovet Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Livestock and Animal Research Jurnal Agripet Animal Production

Tri Eko Susilorini

Faculty Of Animal Science, Brawijaya University, Malang, Indonesia

Author-ID : 396544

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Medicine & Pharmacology Veterinary Other

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Genetic Polymorphism at Acaca Locus and Its Relationship With Productive Performances in Ettawa Crossbred Goat Sucik Maylinda; Tri Eko Susilorini; Puguh Surjowardojo
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Research with aim to estimate genetic polymorphism at ACACA (Acetyl-coenzyme A carboxylase ) locus in Ettawa Crossbred goat wan its relationship with production traits was done at goat population in Batu, Lawang and Ampel Gading. 46 female goats were taken it’s blood sample to isolate the DNA and continue with PCR (Polymerase Chain Reaction) and RFLP (Restricted Fragment Length Polymorphism). PCR was used to amplify ACACA gene fragment in intron 3’ about 200 bp with primer F : 5’ – AGT GTA GAA GGG ACA GCC CAG C – 3’ and R : 5’ – GTG GAA TGA CAC ATG GAG AGG G – 3’; RFLP was used to test mutation of that fragment in particular place (point) using restriction enzyme RSA1. Variables were alelles and genotypes composition in population, milk and fat content, and birth weight of kid. Result showed that (a) genetic polymorphism at locus ACACA in three location was high that is 44,22 %, with allele frequency of G (p) = 33 % and allele T (q) = 67 %; (b) no relationship between the high polymorphism with productive performance of goat in fat and protein content, and birth weight of kid. It was concluded that in goat population there was a high polymorphism at ACACA gene, and that polymorphism was not related to production.

The Effects of Non-Genetic Factors on The Birth Weight, Litter Size and Pre-Weaning Survive Ability of Etawah Cross-Breed Goats in The Breeding Village Center in Ampelgading District Tri Eko Susilorini; Kuswati Kuswati; Sucik Maylinda
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

A total of 106 late gestation goats (age 4-5 months of pregnancy) records each of Etawah Crosbred goats kept on small farmer over a period of 10 years were assessed to determine the effects of environmental factors on them. Body Condition Score is non-genetic factor and in dairy goats to predicted milk production, fertility, and general health of the animal because BCS is visualisasi from feeding manajement. The objective of this study was to investigated the relationship between Body Condition Score (BCS) of late gestation with litter size and birth weight on etawah crossbred goats. The results showed that the average of litter size and birth weight (kg) were 1.89±0.66 and 3.84±0.73 respectively. The relationship between BCS with litter size were 0.13 (very low) and the relationship between BCS with birth weight were 0.11 (very low). The conclusion of this research is BCS of late gestation had positive correlation on litter size and birth weight, however this was low and birth weight as first indicator of future growth rate.

Polymorphism of Growth Hormone Gene in Selecting Etawah Crossbred (PE) Goats Tri Eko Susilorini; Kuswati Kuswati; Sucik Maylinda
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Although Etawah Crossbred (PE) goat is considered to be dual purpose (meat and milk) goat, it is mainly raised for meat production. Since early 1990, there has been a growing interest of the farmer in some places to raise PE goat for milk production without sacrificing its role to produce kids for meat. Although milk yield of PE goat was not as high as milk yield of some other dairy goats, the ability of PE goat to cope with harsh local environment, particularly climate and feed conditions, was an advantage. Therefore, raising PE goat would still be an important part of farmer activities in the rural areas in Indonesia. Identification of the genes underlying livestock production traits leads to more efficient breeding programs and it is a promising way to improve production traits of farm animals. Growth hormone is a polypeptide hormone which is the major regulator of the metabolic procedures of growth and development and it is encoded by GH gene. The objective of this study was to detect the genetic polymorphism of GH gene in major Etawah Crossbred (PE) goat using PCR-RFLP. The PCR amplified fragment were digested with HaeIII endonuclease and the result showed the presence of two genotype CC and CD. The total frequency were 47.0% and 53.0% for CC and CD genotype respectively in 94 tested goats. Statistical analysis showed that in the fragment amplified by the pair of primer, CD genotype had significant higher birth weight and weight of 100 days old (weaning weight) than CC genotype (P<0.01). In conclusion that GH gene may be a mayor gene or linked to the mayor gene to affect the weight traits and the polymorphic site could be used to select the goat weight in marker-assisted selection program.

Co-Authors Abdul Manab Adelina Ari Hamiyanti Aditya Cahya Wardhana Adli, Danung Nur Ahmad Furqon Ahmad Furqon Ahmad Furqon Ahmad Zarkasi Efendi Ali Murthadho Amam Amam Amam Amam Angel Aprilia Panjaitan Anggun Permata Sari Anif Mukaromah Wati Anisa Wulandari Aswah Ridhowi Desinta Wulandari Desinta Wulandari Endang Setyowati Firman Jaya Fitria Ella Wardani Fitria Ella Wardani Gatot Ciptadi Guruh Prasetyo Hanum Muarifa Hanum Muarifah Heri Damayanti Ike Mawarni Handayani Ilmiatus Sholichatunnisa Imam Thohari Inggita Leli Murtika Inggita Leli Murtika K Ghozi Kanzul Kamal Putra Kusumastuti, Anie Eka Kuswati Kuswati Kuswati Kuswati Larasati, Rizka Latifah Hanum Lutfiana Safitri Manik Eirry Sawitri Masdiana Ch. Padaga Mashudi Mashudi Moch Sasmito Djati Muhammad Halim Natsir Muhammad Sanjaya Kusuma Nanda Dwi Putra Nur Hidayah Nurgiartiningsih, V. M. A. Oki Selfiana Marsono Osfar Sjofjan Palayukan, Jisril Paskah Partogi Agung Pramujo, Muhammad Puguh Surjowardojo Puguh Surjowardojo Puguh Surjowardojo Rachmad Dharmawan Ramdhan Riky Winandi Rifa'i Rifa'i Rifa’i Rifa’i Rini Dwi Wahyuni Rini Dwi Wahyuni Sucik Maylinda Sucik Maylinda Sucik Maylinda Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Suyadi Tasya Nur Karina Teguh Wicaksono Umi Wisaptiningsih Vasco Benarivo Widi Nugroho Wike Andre Septian Wike andre Septian Wina Astriyani Yuli Arif Tribudi

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