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All Journal Jurnal Sain Veteriner Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Teknologi Informasi dan Ilmu Komputer Biomedika Majalah Obat Tradisional The Indonesian Biomedical Journal Indonesian Journal of Chemistry Communications in Science and Technology Jurnal Pendidikan Matematika (Kudus) JPSCR : Journal of Pharmaceutical Science and Clinical Research GANEC SWARA Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education JURNAL ANALIS LABORATORIUM MEDIK Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Jurnal Literasi Pendidikan Fisika (JLPF) Jurnal Algoritma Engagement: Jurnal Pengabdian Kepada Masyarakat
Rina Susilowati
Department Of Histology And Cell Biology, Faculty Of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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PENGARUH MASA PENYIMPANAN SAMPEL WHOLE BLOOD DAN BUFFY COAT DARAH TIKUS TERHADAP KUANTITAS DAN INTEGRITAS RNA Winarti, Retno; Nurhidayat, Annisa Ramadhani; Purnomosari, Dewajani; Suhda, Saihas; Susilowati, Rina
Jurnal Analis Laboratorium Medik Vol 10 No 2 (2025): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

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Abstract

Latar belakang: Whole blood dan buffy coat merupakan dua sumber sampel yang sering digunakan dalam penelitian molekuler. Namun, kualitas RNA yang dihasilkan seringkali kurang memuaskan. Faktor yang diperkirakan dapat mempengaruhi kualitas RNA adalah jenis sampel dan kondisi penyimpanan. Tujuan: untuk mengetahui pengaruh masa penyimpanan sampel whole blood dan buffy coat terhadap kuantitas dan integritas RNA, membandingkan kedua tipe sampel. Sampel darah dari tikus Sprague dawley umur 3 bulan diambil melalui sinus retro-orbitalis dan cardiac punture lalu dipisahkan sebagai whole blood dan buffy coat dan disimpan pada suhu −80°C selama 1 hari, 1 minggu, 1 bulan, dan 3 bulan. RNA diisolasi menggunakan kit Quick-RNA Miniprep Plus Zymo dan diperiksa konsentrasi serta kemurniannya dengan spektrofotometer (A260/A230 dan A260/A280). Integritas RNA diperiksa dengan elektroforesis agarose dan dibaca menggunakan Gel Doc. Metode: Data dianalisis menggunakan uji Two-way ANOVA. Analisis menunjukkan bahwa tipe sampel merupakan faktor utama yang mempengaruhi kuantitas dan integritas RNA. Hasil: Buffy coat menghasilkan konsentrasi RNA yang lebih tinggi dibanding whole blood (p < 0,0001). Faktor lama penyimpanan hingga 3 bulan pada suhu −80°C tidak mempengaruhi konsentrasi RNA pada kedua jenis sampel. Integritas RNA buffy coat lebih baik dibandingkan whole blood. Kesimpulan: Hal ini ditunjukkan oleh hasil elektroforesis pita RNA buffy coat yang lebih jelas dan konsisten. Masa penyimpanan buffy coat dan whole blood pada suhu −80°C sampai dengan 3 bulan tidak mempengaruhi kuantitas dan kemurnian RNA, namun mempengaruhi kualitas RNA.
Therapeutic Potential of White Sweet Potato Peel in Enhancing Insulin Production and The Proliferation of Beta Cells in Diabetic Rats Royhan, Aan; Susilowati, Rina; Sunarti, Sunarti
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 11, No 1 (2026)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v11i1.94516

Abstract

Diabetes mellitus (DM) affects approximately 422 million people worldwide, predominantly in low- to middle-income countries, and is associated with increasing morbidity and mortality. The long-term use of conventional antidiabetic drugs may cause adverse effects, prompting interest in natural therapeutic alternatives. This study aimed to evaluate the effects of white sweet potato peel (WSPP) on glycemic control, insulin expression, and pancreatic beta-cell proliferation in streptozotocin-induced diabetic rats. Male Wistar rats were divided into five groups: diabetic control, diabetic rats treated with WSPP at doses of 400, 800, and 1600 mg/kgBW/day, and non-diabetic controls. Diabetes was induced by a single intraperitoneal injection of streptozotocin (60 mg/kgBW). Fasting blood glucose (FBG) levels were measured at baseline and during treatment. Pancreatic tissues were analyzed using immunohistochemistry to assess insulin expression, Langerhans islet area, and beta cell proliferation using anti-insulin and anti-PCNA antibodies. WSPP administration resulted in a significant, dose-dependent reduction in FBG levels compared to diabetic controls (p < 0.05). The highest dose (1600 mg/kgBW/day) reduced FBG from approximately 250 mg/dL to 72.22 mg/dL after four weeks (p < 0.01), reaching the normal range. Immunohistochemical analysis demonstrated significant increases in insulin expression scores, Langerhans islet area, and the percentage of PCNA-positive cells in the 800 and 1600 mg/kgBW/day groups compared to untreated diabetic rats. At the highest dose, these parameters approached those observed in non-diabetic controls, indicating substantial restoration of pancreatic islet structure and function. In conclusion, WSPP improves glycemic control and promotes beta-cell proliferation in diabetic rats, highlighting its potential as a natural, cost-effective therapeutic strategy for diabetes management.
Co-Authors Aan Royhan Adi Saputra Adika Zhulhi Arjana Aflifia Birruni Sabila Agung Endro Nugroho Agung Endro Nugroho Agustin, Vita Ahmad Hamim Sadewa Anak Agung Istri Sri Wiadnyani Andwi Ari Sumiwi Arief Nurrochmad Arta Farmawati Asysyifa, Azzahra Christine Ayu Lagonda David Pakaya Dewajani Purnomosari Dewajani Purnomosari, Dewajani Dewi Kartikawati Paramita Dhoriva Urwatul Wutsqa Dian Caturini Sulistyonigrum Dian Eurike Septyaningtrias Dicky Moch Rizal Dicky Moch Rizal Dilafitria Fauza Dwi Aris Agung Nugrahaningsih Dwi Cahyani Ratna Sari E. Elsa Herdiana Murhandarwati Elizabeth Henny Herningtyas Emhandyksa, Medycha Emy Huriyati Eti Nurwening Sholikhah Fajar Shodiq Permata Fifteen Aprila Fajrin Ginus Partadiredja Hanggoro Tri Rinonce Ignatius Ryan Adriawan Ignatius Ryan Adriawan, Ignatius Ryan Indah Soesanti Jajah Fachiroh Jajah Fachiroh, Jajah Jaya, Sudi Indra Julkarnain, M Mardinata, Erwin Mohamad Andrie Muhammad Ghufron Muhammad Mirandy Pratama Sirat Ninda Devita, Ninda Nurhidayat, Annisa Ramadhani Nurrahma, Bira Arumndari Pipin Dwi Kartikasari Ramadhani Nurhidayat, Annisa Ratih Feraritra Danu Atmaja Rini Dharmastiti Santoso, Fendi Satrio Wicaksono Suhda, Saihas Sunarti Suwidjiyo Pramono Suwidjiyo Pramono Syarifa, Cut Alima Teguh Triyono Titik Nuryastuti Tri Ratnaningsih Umi S. Intansari Ida Savitri Laksanawati Veronika Maria Sidharta Wibowo, Ghina Tamima Widodo, Irianiwati Widyaningtyas, Febrina Siska Winarti, Retno Wiwit Setyowati Yuliani, Aska Muta Yuyus Kusnadi