This study aimed to implement the Cardrat (Carbohydrate) learning media, developed using the “learning by asking” approach and supported by App Inventor, on the biomolecule carbohydrate topic to improve student learning outcomes. The research employed a quasi-experimental design with participants consisting of Science Education students at the Faculty of Teacher Training and Education, University of Bengkulu, who had completed the Biochemistry course. The instruments used included validation questionnaires, readability tests, and pretest posttest assessments. Validation results indicated that the media was categorized as highly feasible, with scores of 73 and 56 for material and media aspects, respectively. The readability test produced an average score of 83.67, falling into the “very good” category. In terms of learning outcomes, both groups showed improvement. In the control class, the average score increased from 34.52 to 76.54 (N-gain 0.64), while in the experimental class it rose from 35.46 to 80.23 (N-gain 0.69). These findings demonstrate that the Cardrat media, based on a learning-by-asking approach and supported by App Inventor, is more effective in enhancing learning compared to conventional methods.