Article Per Year (5 Year)
p-Index From 2020 - 2025
2.81
P-Index
This Author published in this journals
All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Journal of Natural Sciences and Mathematics Research Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Current Biomedicine
Nugraha, Arifin Budiman
Departemen Ilmu Penyakit Hewan Dan Kesmavet, Fakultas Kedokteran Hewan, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary Other

Published : 19 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search
Journal : Jurnal Sain Veteriner

 1 
Perlakuan Celup Puting setelah Pemerahan terhadap Keberadaan Bakteri Patogen, Staphylococcus aureus, Streptococcus agalactiae, dan E. coli pada Sapi Perah Penderita Mastitis Subklinis di Peternakan KUNAK Bogor Herwin Pisestyani; Etih Sudarnika; Rachmi Ramadhanita; Abdul Zahid Ilyas; Chaerul Basri; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Mirnawati B Sudarwanto1
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.728 KB) | DOI: 10.22146/jsv.29293

Abstract

Mastitis is udder inflammation that can be caused by the entry of pathogenic bacteria through the teat. One of the treatments that can prevent bacteria infection is teat dipping. This research was aimed to identify the effectof teat dipping after milking to the presence of pathogenic bacteria that cause subclinical mastitis (Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli) and the presence of pathogenic bacteria that cause subclinicalmastitis by quarter of udder. Milk samples were taken from normal lactation period of dairy cows, clinically healthy, and positive subclinical mastitis status when the intervention was applied. Sixty seven samples of quarters from 18dairy cows were identified to the presence of pathogenic bacteria that cause subclinical mastitis before teat dipping and at 1st, 2nd, and 3rd months after teat dipping. Identification of the presence of pathogenic bacteria was done byculturing samples on selective agar media. The presence of common pathogenic bacteria, Staphylococcus aureus, and Escherichia coli decreased significantly after teat dipping, while Streptococcus agalactiae and other pathogenicbacteria did not decrease significantly. The presence of pathogenic bacteria based on the quarter of udder did not show the significance difference. Application of teat dipping after milking was able to decrease the presence ofpathogenic bacteria that cause subclinical mastitis.
Prevalence and risk factors of Cryptosporidium spp. on dairy farms in Bogor Arifin Budiman Nugraha; Umi Cahyaningsih; Etih Sudarnika
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.55961

Abstract

Cryptosporidial infection is one of the most common causes of diarrhea in humans and livestock worldwide. This study was conducted to estimate the prevalence of Cryptosporidium infection and to identify potential risk factors associated with shedding of oocysts in Bogor. A total of 308 faecal samples were collected from 136 calves less than 6 months, 44 from those 6-12 months and 128 from those than 12 months. Data of factors potentially associated with the likelihood of Cryptosporidium spp. infection were recorded (i.e., enviromental status, size of herd, and herd management). Cryptosporidium spp. oocyst was identified by using modified acid fast (Ziehl Neelsen) staining technique and microscopically examined under 400x magnifition. Results showed that the prevalence of cryptosporidiosis in Bogor was 21.1% (CI 95%; 16.5%-25.6%). The highest prevalence was 29% (CI 95%; 26.8%-31.7%) in cattle aged less than 6 months. The oocysts abundance were around <5 oocysts per microscopy visual area. Data was analyzed using logistic regression models.  Statistical analysis showed that there were association between cryptosporidiosis and calves aged less than 6 months with an odds ratio (OR) of 2.7 (CI 95%; 1.5-5.2) times compared with cattle aged more than 12 months.
Parasit Gastrointestinal pada Kadal Naga Berjanggut (Pogona vitticeps) di Cijeruk, Bogor Nisrina Rosyida Noor Rifai; Arifin Budiman Nugraha; Ekowati Handharyani; Fajar Kawitan; Umi Cahyaningsih
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.80391

Abstract

Bearded dragons are reptiles that are quite popular as pets. There are many factors of disease that can infect bearded dragons. The environment and feed affect the level of disease threat, such as parasitic infection in the gastrointestinal tract. Data information related to parasitic gastrointestinal infections in bearded dragons in Indonesia is still limited, so supporting examinations are necessary to help diagnose. A total of 38 fecal samples were collected from the bearded dragon in Cijeruk Bogor. Samples were examined by simple flotation method, Ziehl Neelsen staining, and counting the number of parasitic eggs. All samples were positive for gastrointestinal parasite infections such as Choleoeimeria sp., Isospora amphiboluri, Cryptosporidium spp., ascarid egg, and oxyurid egg. The average number of Choleoeimeria sp. and Isospora amphiboluri oocysts were 8730 and 51.281 oocysts per gram of feces. The average number of helminth eggs per gram was 290 and 2325 for ascarid and oxyurid, respectively. Most of the infected bearded dragons do not show significant clinical symptoms. The number of oocysts and worm eggs per gram of feces showed mild and high results.
Leukocyte Profile of Mice Infected with Plasmodium berghei and Treated with Aqueous Extract of Strychnos Ligustrina and Its Combination with Dihydroartemisinin Piperaquine Phosphate (DHP) Umi Cahyaningsih; Siti Sa&#039;diah; Wasrin Syafii; Rita Kartika Sari; Arifin Budiman Nugraha
Jurnal Sain Veteriner Vol 41, No 3 (2023): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.88896

Abstract

Malaria is still one of the most highly pathogenic diseases, which remains a significant problem for public health. Natural compounds are alternative ways as an antimalarial compound that are applied for the artemisinin combination. The aim of this study was to determine the effect of a combination of S. ligustrina extract and DHP on the differential leukocytes percentages. This study used 100 mice which were divided into five groups. Group A and B were used for the healthy mice and infected-untreated groups, respectively. Group C used as a controlled drug, received 222 mg/kg BW of DHP. Group D received 300 mg/kg BW of aqueous extract of S. ligustrina. Group E received a combination between 111 mg/kg BW of DHP and 200 mg/kg BW of aqueous extract S. ligustrina. The findings demonstrated that DHP and S. ligustrina extract had an impact on neutrophils, lymphocytes, inhibition, and percentages of parasitemia. The findings revealed that treatment groups C, D, and E reduced the percentage of parasitemia. The percentage of neutrophils in treated groups was significantly higher than in group A (P<0.05). The percentage of lymphocytes was lower in the treated groups, while the percentages of eosinophils, monocytes, and basophils did not significantly different (P<0.05). This study found that group D and E had the same effect on the leucocyte profile as group C. Our findings suggest that the aqueous extract of S. ligustrina, as well as the combination treatment with DHP, have the potential to lead to the discovery of new antimalarial medicines.
Comparison of Morphology and Protein Profile of Acetone and PBS-Fixed Toxoplasma gondii Tachyzoites for Detection of IgG and IgM Seropositivity VALINATA, SISCA; Cahyaningsih, Umi; Nugraha, Arifin Budiman; Kurniawati, Dyah Ayu; Desem, Muhammad Ibrahim; Fong, Sulinawati
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103155

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes zoonotic diseases. The prevalence of toxoplasmosis in humans in Indonesia ranges from 9.7-70%. The parasite is difficult to detect in tissues; therefore, serological testing is the most common method for detecting antibodies against Toxoplasma. Antibodies are often used as the testing criteria for IgG and IgM antibodies. Previous research has shown that agglutination testing with acetone-fixed tachyzoites was only positive for acute infection. The aim of this study was to examine microscopic changes in acetone-fixed tachyzoites, determine their ability to detect IgG and IgM seropositivity, and detect specific proteins for IgG and IgM against toxoplasmosis. Tachyzoites were fixed with acetone (A) and PBS (P). After fixation, tachyzoites were prepared to observe the morphology and sonicated to obtain Soluble Toxoplasma Antigen (STA). STA was then subjected to SDS page and western blotting. The addition of aseton resulted in morphological and protein changes. Although changes occur, acetone-fixed tachyzoites can still react with IgG and IgM seropositivity. Protein bands that can be used as IgG seropositive markers in western blot testing are bands measuring 20, 24, 27, 73, and 110 kDa, and the combination of acetone fixation with anti-goat IgM conjugate will result in seropositive bands.
Pengembangan Deteksi Parasit Darah Theileria equi dan Babesia caballi secara Otomatis menggunakan Algoritma YOLOv8 Arif, Ridi; Nugraha, Arifin Budiman; Kedaton, Feni Gemala; Erlangga, Wishnu Kusumo Agung; Wibowo, Bagas Dwi Suryo
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.104069

Abstract

Piroplasmosis pada kuda merupakan penyakit parasit darah yang disebabkan oleh Theileria equi dan Babesia caballi, menyebabkan anemia, ikterus, kegagalan organ, serta pembatasan aktivitas kuda, termasuk larangan mengikuti kompetisi. Deteksi piroplasmosis secara rutin masih mengandalkan pemeriksaan mikroskopis ulas darah untuk melihat morfometrik parasitnya sehingga membutuhkan keahlian tinggi dan memerlukan waktu relatif lama. Penelitian ini bertujuan mengembangkan sistem deteksi otomatis parasit T. equi dan B. caballi berbasis algoritma YOLOv8 untuk meningkatkan kecepatan dan akurasi identifikasi mikroskopis. Pengambilan data dilakukan dengan pembuatan preparat ulas darah dari kuda yang terdiagnosis positif piroplasmosis yang telah divalidasi oleh ahli dari Laboratorium Protozoologi SKHB IPB. Preparat diwarnai menggunakan giemsa 10%, dilakukan pengambilan gambar, pembuatan dataset, anotasi data, pelatihan model YOLOv8, serta pengujian performa sistem. Hasil penelitian menunjukkan performa model mendeteksi parasit dengan mAP50 sebesar 69,8%, mAP50-95 sebesar 40,5%, dan kecepatan deteksi 5,4 ms. Evaluasi performa manual menunjukkan akurasi 91%, presisi 98%, recall 92%, dan F1-score 95% dibandingkan pemeriksaan mikroskopis. Penelitian ini menyimpulkan bahwa sistem berbasis YOLOv8 mampu melakukan deteksi T. equi dan B. caballi secara otomatis dengan presisi tinggi dan waktu deteksi cepat sehingga berpotensi digunakan sebagai alat bantu diagnosis piroplasmosis yang lebih efisien.
Co-Authors Abdul Zahid Adistya, Erly Rizka Afiff , Usamah Agus Wijaya Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Aviola, Lala Azizah, Hanifah Nur Azmi, Zul Budiono, Novericko Ginger Chaerul Basri Delimasari, Zahrizada Zamrudina Denny Widaya Lukman Denny Widya Hikman Desem, Muhammad Ibrahim Didik Tulus Subekti Dwi Utari Rahmiati Edi Sukmawinata Edi Sukmawinata Ekowati Handharyani Elmanaviean, Muhammad Erlangga, Wishnu Kusumo Agung Etih Sudarnika Fajar Kawitan Fitri, Arni Diana Fitrine Ekawasti, Fitrine Fong, Sulinawati Gusdinar, Rizal Hadri Latif Hammada Raudlowi Herwin Pisestyani Iccha Pradipta Patsiwi Jerica, Monica Silva Kedaton, Feni Gemala Ketut Adnyane Mudite koekoeh santoso Kurniawati, Dyah Ayu Mayasari, Ni Luh Putu Ika Milna, Resi Mirnawati B Sudarwanto1 Mirnawati Sudarwanto Mokhamad Fahrudin Muhammad Pauzi Lubis Murtiningrum, Fitria Senja Ni Wayan Kurniani Karja Nisrina Rosyida Noor Rifai Qing Wen, Jyn Soong R. Iis Arifiantini Rachmi Ramadhanita Ridi Arif Risa Tiuria Rita Kartika Sari Ronald Tarigan Rotinsulu, Dordia Anindita Sadiah, Siti Satyawardana, Wirokartiko Silvarajoo, Keertana Siti Sa&#039;diah sri murtini . Subangkit, Mawar Tara Puri Ducha Rahmani Tuliman, Nurul Annisa Ulfatin Khoiriyah Herowati Umi Cahyaningsih Valinata, Sisca Veenu Kumar Veerasamy, Suganti Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wasrin Syafii Wibowo, Bagas Dwi Suryo Yusuf Ridwan