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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Innovation and Applied Technology Research Journal of Life Science Plantropica: Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Cakrawala: Jurnal Litbang Kebijakan Jurnal Entomologi Indonesia Jurnal HPT (Hama Penyakit Tumbuhan) Journal of Fisheries & Marine Jurnal Pengabdian Masyarakat SAPANGAMBEI MANOKTOK HITEI
Hagus Tarno
Jurusan Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Medicine & Pharmacology Nursing Social Sciences Other

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Nematicidal Activity of Turmeric Extract against Nematodes Meloidogyne spp. Ulfa, Maria; Himawan, Toto; Tarno, Hagus
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.7

Abstract

In an effort to reduce the impact of synthetic nematicides, a new formula was developed using plant-based nematicides that are more environmentally friendly, one of the basic ingredients that can be used is turmeric. The purpose of this study was to determine the effectiveness of turmeric extract (Curcuma domestica) with various different solvents namely aquades, methanol, and n-hexane at concentration of 5%, 10%, 15% and 20% respectively in inhibiting the activity of Meloidogyne spp. The experiment used was a Completely Randomized Design (CRD). Each treatment was repeated 4 times with 4 controls so that there were 52 experimental units. The result of different research data was analyzed using Analysis of Variance (ANOVA) which was then further tested with Honest Significant Difference (HSD) at the 5% level if real, and t-test for in vivo test. Based on the tested extract, it was found that turmeric extract with various solvent (aquades, methanol, and n-hexane) was effective in inhibiting the activity of Meloidogyne spp. nematodes outside the roots (hatching eggs and penetration), turmeric extract didn’t affect the activity of Meloidogyne spp. in the roots (development and reproduction), vegetable nematicides which the most active in suppressing the population and inhibiting the activity of nematodes was turmeric extract using methanol as a solvent at a concentration of 20%.
Abundance and Diversity of Fruit Flies Species (Diptera: Tephritidae) in Bogor and Depok District Octavia, Evy; Tarno, Hagus; Himawan, Toto; Setiawan, Yogo
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.7

Abstract

Fruit flies (Diptera: Tephritidae) are important pests of horticultural crops. This study investigates the abundance and species richness of Bogor and Depok District as a center area horticulture commodity based on Cue Lure Trap. Each Cue Lure Trap was observed to record the abundance and species richness of fruit flies at seven days intervals from June until August 2021 in Bogor District (Babakan, Mekarsari and Parigi Mekar) and Depok District (Bedahan and Pancoran Mas), West Java, Indonesia. A total of 1,025 individuals were collected and consist of nine species of fruit fly i.e., Bactrocera albistrigata, B. carambolae, B. neocognata, B. verbascifoliae, Dacus conopsoides, D. longicornis, Zeugodacus calumniata, Z. caudatus, and Z. cucurbitae. The number of individuals of fruit flies was highest in the Bedahan, Depok District (F = 5.454, P = 0.002). Zeugodacus cucurbitae was the most abundant species (792 individuals), representing 77.27% of the specimens collected during the sampling period. The highest Shannon–Wiener diversity index of all fruit flies captured in this study was in Bedahan, Depok (1.11) and the lowest in Parigi Mekar, Bogor (0.23). A Bray–Curtis analysis showed that the species compositions of fruit flies on Bedahan and Parigi Mekar had a similarity of 70.1%.
Diversity and Abundance of Insect on Shallot (Allium Ascalonicum L.) with Integrated Pest Management (IPM) and Conventional Patterns Manna, Dorkas Wantiawati; Afandhi, Aminudin; Tarno, Hagus
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.3

Abstract

Research on diversity and abundance of insect on shallot with Integrated Pest Management (IPM) and conventional patterns has been carried out. This study aims to analyze diversity and abundance of insects, damage level of shallot, and relationship between diversity and abundance of insects with growth and production of shallot in IPM and conventional land. This research was conducted in Junrejo village, Batu City in September to December 2017 while identification of insect was carried out at the Laboratory of Plant Pest and Disease, University of Brawijaya. This study uses Yellow pan trap and Pitfall trap methods. The result showed diversity index (H') of IPM land was 2.50 and of conventional land was 2.67, which both are moderate diversity. The abundance of insect on IPM land is higher (8672 individuals) compared to conventional land (3475 individuals). The level of crop damage is lower in IPM land compared to conventional land, while shallot growth and production is higher in IPM land compared to conventional land.
Forecasting Model of Coffee Berry Borer (Hypothenemus Hampei) in Pasuruan District Nadiah, Annisrien; Himawan, Toto; Tarno, Hagus
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.2

Abstract

Coffee, a globally important commodity, faces difficulties in Indonesia due to pests that reduce its quality and productivity. The coffee berry borer (Hypothenemus hampei) is particularly dangerous, causing hollowed-out coffee seeds and causing massive annual losses worldwide and in Indonesia. Factors such as climate, weather, and other environmental aspects play a pivotal role in influencing pest lifecycles, emphasizing the necessity of understanding the intricate relationship between pests and their environment for effective management. This research aims to contribute to minimizing coffee production losses and harvest failures resulting from H. hampei infestations. This study relies on secondary data from the BBPPTP in Surabaya and NASA. Using panel data regression analysis, the study discovers that coffee berry borer attacks in the Pasuruan District follow a seasonal pattern. Furthermore, with a forecasting accuracy of 15.94%, the model reveals that air humidity, temperature, and previous attacks are the dominant variables influencing current coffee berry borer attacks. The developed model provides a reliable tool for forecasting coffee berry borer attacks, with humidity and previous month's attacks emerging as the most influential factors, particularly in the Pasuruan District of East Java.
Biodiversity and Distribution Rice Arthropods in Malang Indonesia Istiqomah, Rina Nur; Tarno, Hagus; Mudjiono, Gatot
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.5

Abstract

Climate change is closely related to changes in abiotic conditions. These changes could have an impact on agricultural cultivation and affect the existence of agricultural arthropods. Different environmental conditions will influence the distribution patterns, richness and diversity of arthropods living in cultivated land. The research was conducted at 8 rice cultivation locations in Greater Malang. Observation of arthropods using pitfall and yellow pan traps, with a diagonal pattern. The total arthropods obtained were 3,937 individuals, 39 families, 46 genera and 48 species. The highest predator species found were Micraspis discolor, the herbivore Nilaparvata lugens, other insect is Culista incidens, and the predator is Drynus baegamensis. The highest index analysis for diversity in Lowokwaru H'= 3,075 in the high category, evenness in Karangploso E=0,864 in the high category and dominance in Pagelaran D=0,179 in the low category. PCA analysis of species composition divided into 2 habitat types, lowland and medium land groups. Species richness increased with increasing observation locations. Environmental factors will bring in species that are suitable for the habitat. Further research needs to be carried out regarding ecological variables or environmental factors, which affect the patterns composition and species richness of arthropods.
New Report: Helopeltis cinchonae Mann (Hemiptera: Miridae) Attacking Commercial Hydrangea Flowering Plants in East Java, Indonesia Suputa, Suputa; Sari, Riya Fatma; Tarno, Hagus
AGRIVITA Journal of Agricultural Science Vol 47, No 1 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i1.4669

Abstract

Formerly, the mirid bug has not been recorded for Hydrangea macrophylla (Thunb.), in Indonesia. Infestation of mirid bug was found on the leaves of Hydrangea in the vegetative growth phase from July 2023 to July 2024 in five hamlet fields at the ornamental farming center in Batu City, East Java. Plant samples were observed diagonally and five plants in each point were selected for samples. On each of the top ten leaves of the sample plants, the percentage of attack symptoms was carefully observed. Nymphs found during the observation were reared to imago, and adults were identified as Helopeltis cinchonae Mann (Hemiptera: Miridae). Insect pests, infestation symptoms, and egg locations were documented during the study. This is the first report of a pest causing significant damage to commercial Hydrangea plants in Batu, East Java, indicating the need to develop an integrated pest management strategy to support increased Hydrangea cut flower production in Batu. 
Use Of Organic Fertilizer and IPM in Certified Organic Rice Farming in East Java Winarno, Dinariningrum Rahma; Tarno, Hagus; Mudjiono, Gatot
Research Journal of Life Science Vol 11, No 1 (2024)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2024.011.01.2

Abstract

This study aims to analyze the effect of organic fertilizer use and Integrated Pest Management (IPM) on the success of certified organic rice farming in East Java. The research method used is descriptive quantitative with Structural Equation Modeling-Partial Least Squares (SEM-PLS) analysis. Data was collected by surveying 100 organic rice farmers in Kediri District. The results showed that the use of organic fertilizers and IPM significantly increased the productivity of organic rice. Organic fertilizers, such as compost and animal manure, can improve soil fertility and crop yields. Meanwhile, IPM helps control pests without using chemical pesticides. The SEM-PLS model confirmed that both variables positively and significantly influence the success of organic rice farming. The results also show that farmers who implement organic farming have a higher level of environmental awareness and are more concerned about consumer health. However, some challenges are still faced, such as limited market access and farmers' lack of knowledge about organic farming techniques. Therefore, more intensive efforts are needed to provide education and training to farmers, as well as to develop policies that support the development of organic farming. In conclusion, the application of organic fertilizer and IPM is the key to the success of organic rice farming in East Java. With the right support, organic farming can be a sustainable solution to increase productivity, preserve the environment, and improve farmers' welfare.
Altitude-Dependent and Host Variety in Fruit Fly Populations: A Study on Bactrocera spp. in East Java Hamdoen, Fadel Muhammad; Tarno, Hagus; Syamsulhadi, Mochammad; Gandaseca, Seca
Research Journal of Life Science Vol 11, No 1 (2024)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Oriental fruit fly (Bactrocera spp.) are one of the most invasive pests, known for their severe impact on causing damage to wide variety of comodities. In Indonesia, as an agricultural country, possesses vast agricultural areas, in which Bactrocera fruit flies pose a persistent threat to production, particularly in regions known for horticulture. This study aims to identify key environmental drivers influencing their distribution and abundance. A cross-sectional study was conducted across thirteen locations located in four regencies and cities within East Java, Indonesia. Fruit fly samples were collected using a purposive random sampling method, with the quantity of samples adjusted to the fruit availability at the research site. The results showed that B. albistrigata, B. carambolae, and B. dorsalis exhibit strong ecological overlap, sharing similar host plants and environmental preferences, which facilitate their coexistence. Furthermore, the research provides a foundation for predicting potential shifts in population distribution in response to climate change, enabling proactive measures to safeguard vulnerable agricultural systems.
Seagrass Ecosystem Assesment for Dugong Conservation: Integrating Anthropogenic Activites and Oceanographic Parameters in East Java’s Coastal Waters Dewi, Citra Satrya Utama; Ciptadi, Gatot; Wahyudi, Slamet; Wiadnya , Dewa Gede Raka; Semedi, Bambang; Sukandar, Sukandar; Isdianto, Andik; Sunuddin, Adriani; Herandarudewi, Sekar Mira; Tarno, Hagus
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.71841

Abstract

Graphical Abstract   Highlight Research 1. The study successfully identified the utilization of coastal and marine areas within the research site. 2. Oceanographic parameters across the study area have been analyzed, providing insights into variations in environmental conditions favoring seagrass ecosystem resilience. 3. The structure of seagrass vegetation has been thoroughly observed, revealing differences in species composition, density, and coverage. 4. Furthermore, the study establishes the connectivity between coastal and marine spatial utilization, oceanographic factors, and seagrass ecosystem dynamics, highlighting their interdependent relationships.     Abstract This study aims to assess the ecological status of seagrass meadows and their relationship with dugong (Dugong dugon) habitats across four distinct ecological regions in East Java, Indonesia. Field assessments were conducted to evaluate seagrass community structure, oceanographic parameters, and the intensity of human activities. Seagrass distribution was mapped using Sentinel-2A satellite imagery, while seagrass health was evaluated through the Seagrass Ecological Quality Index (SEQI) and Importance Value Index (IVI). Statistical analyses, including Analysis of Variance (ANOVA) and Principal Component Analysis (PCA), were employed to identify significant differences and key environmental drivers. The results revealed marked spatial variation in oceanographic characteristics and seagrass coverage (p < 0.05). PCA showed that anthropogenic factors—particularly marine space utilization such as aquaculture and coastal development—were major contributors to seagrass degradation. Elevated nutrient concentrations were also associated with declining seagrass health, indicating land-based pollution as a dominant stressor. These findings underline the urgent need for integrated coastal zone management. Strengthening conservation policies, reducing terrestrial runoff, and implementing sustainable marine spatial planning are critical to safeguard seagrass ecosystems and ensure the long-term survival of dugong populations in Indonesian waters.
The Potential of Endophytic Fungi Producing Hydrolytic Enzymes as Biocontrol Agents of Alternaria porri Causing Onion Purple Blotch Firdausi, Wita; Abadi, Abdul Latief; Aini, Luqman Qurata; Martosudiro, Mintarto; Tarno, Hagus
AGRIVITA Journal of Agricultural Science Vol 47, No 3 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i3.4924

Abstract

Purple blotch caused by Alternaria porri is the primary disease in shallots, causing yield losses of 30-100%. The use of endophytic fungi as biocontrol agents has several advantages, including adaptability to host plants, selective targeting of pathogenic fungal cell walls without harming plant cells, and environmental friendliness. This study aims to select endophytic fungi producing hydrolytic enzymes with the highest inhibitory effect against A. porri. Endophytic fungi were isolated from the Batu Ijo local variety of shallots in Dau District, Malang, then evaluated for antagonism and for their ability to produce hydrolytic enzymes. This study obtained 42 isolates, consisting of 25 fungi and 17 yeasts. The endophytic fungi with the highest inhibitory activity were identified as Trichoderma asperellum (71.4%) and Pichia onychis (70.04%). T. asperellum produced cellulase at concentrations of 16.78 µg/ml and chitinase at 3.85 µg/ml, while P. onychis produced cellulase at concentrations of 11.47 µg/ml and chitinase at 1.56 µg/ml. Microscopic observation of A. porri hyphae showed that both endophytic fungi caused lysis with hyphal fragmentation, discoloration, and deformation. The endophytic fungi T. asperellum and P. onychis were proven to inhibit A. porri in vitro through their ability to produce hydrolytic enzymes that caused A. porri hyphal lysis.
Co-Authors Abdul Latief Abadi Adriani Sunuddin Afiyanti, Mufidah Agus Nugroho, Agus Ainul Hayat Aji Santoso Aji Santoso Akhmad Rizali Alvan Fajarudin Amin Setyo Leksono Aminudin Afandhi Aminudin Afandhi Aminudin Afandhi Andik Isdianto Anggraeni, Alifia Nurma Annike Putri Damayanti Arifin Noor Sugiharto Arin, Iftita Yustitia Arum Yuli Kristanti Bambang Semedi Bambang Tri Rahardjo Bambang Tri Rahardjo Baroro, Ismatul Budi, Setyo DAMAYANTI BUCHORI Dede Durahman Dede Durahman Devi, Mia Prastika Dewi, Citra Satrya Utama Dwi Wulandari Edson Begliomini Eko Wahyu Budi Darmawan Eko Wahyu Budi Darmawan Estri Pamungkasih Fadhila Hasna Fauziah, Septia Hana Fery Abdul Choliq Firdausi, Wita Gatot Ciptadi Gatot Mudjiono Gunawan Prayitno Hamdoen, Fadel Muhammad Handoko Handoko Hari Sutrisno Hari Sutrisno Hasan Suprapto Hauroinsiyah, Fatimah Havinda Anggrilika W.S. Herandarudewi, Sekar Mira Hermawan, Romy Hongye Qi Irvan Kurniawan Isna Kartika Wati Istiqomah, Rina Nur Johannis, Midzon Kafif Andani Karisma Aditya Wardani Karuniawan Puji Wicaksono Karuniawan Puji Wicaksono Karuniawan Puji Wicaksono Karuniawan, Anggik Kazuyoshi Futai Liza Afifah Liza Afifah Liza Afifah Luqman Qurata Aini Manna, Dorkas Wantiawati Maria Ulfa Maris Purnanto Maris Purnanto Masahide Kobayashi Maspupah Huzni Maspupah Huzni Meinardhy, Ricky Mintarto Martosudiro Moch. Dawam Maghfoer Mudji Santosa Muhammad Anton Astoni Muhammad Febriansyah Nadiah, Annisrien Octavia, Evy Presti Mardiyani Purwaningtyas Presti Mardiyani Purwaningtyas, Presti Mardiyani Primastya Dinarwika Primastya Dinarwika Rahmadina Fitria Ristanti Ramayanti, Kinta Retno Dyah Puspitarini Ria Febrianasari Ria Febrianasari Rina Rachmawati Rizki Puji Widiastuti Rizki Puji Widiastuti Ronny Pamuji Ronny Pamuji Ryoko Hirata Sari, Riya Fatma Sari, Rose Novita Satoshi Ito Seca Gandaseca Senoaji, Wasis Silvi Ikawati Silvi Ikawati, Silvi Slamet Wahyudi Sofia Ery Rahayu Sri Heriza Sri Heriza Sri Wahyuni Sudiarso Sudiarso Sukandar Sukandar Sulthoni, Fahmi Suputa Suputa Suyono, Tri Syamsulhadi, Mochammad Taufiqurrahman, Achmad Fitriadi Theresia Rani Kartika Ayu Toto Himawan Toto Himawan Toto Himawan Tsuyoshi Kajisa Wahyudie, Diyan Eko Wasiska Iyati Wiadnya , Dewa Gede Raka Winarno, Dinariningrum Rahma WINDRA PRIAWANDIPUTRA, WINDRA Yahya, Minhajul Qowim Yasa Palaguna Umar Yasushi Mitsuda Yogo Setiawan Zeni Ningrum Zeni Ningrum Zulfaidah Penata Gama