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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Innovation and Applied Technology Research Journal of Life Science Plantropica: Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Cakrawala: Jurnal Litbang Kebijakan Jurnal Entomologi Indonesia Jurnal HPT (Hama Penyakit Tumbuhan) Journal of Fisheries & Marine Jurnal Pengabdian Masyarakat SAPANGAMBEI MANOKTOK HITEI
Hagus Tarno
Jurusan Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Medicine & Pharmacology Nursing Social Sciences Other

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PENGARUH PEMBERIAN PLANT GROWTH PROMOTING RHIZOBACTERIA (Psudomonas fluorescens) TERHADAP NEMATODA PURU AKAR Meloidogyne sp. PADA TANAMAN TOMAT Annike Putri Damayanti; Bambang Tri Rahardjo; Hagus Tarno
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 6 No. 1 (2018)
Publisher : Universitas Brawijaya

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Abstract

Nematoda puru akar (Meloidogyne sp.) merupakan salah satu penyakit penting pada tanaman tomat. Penyakit ini dapat  menyebabkan kerusakan secara kualitatif maupun kuantitatif. Oleh karena itu, tindakan pencegahan perlu dilakukan sebelum serangan nematoda puru akar ini semakin meluas. Penggunaan musuh alami nematoda yang berasal dari kelompok organisme, seperti bakteri dapat digunakan sebagai agen hayati. Penelitian ini bertujuan untuk mengetahui kemampuan isolat bakteri P. fluorescens (UB_Pf1) dalam mengendalikan nematoda puru akar. Penelitian ini dilaksanakan di Rumah Kasa dan Laboratorium Hama Tumbuhan, Sub Laboratorium Nematologi, Jurusan Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas  Brawijaya Malang.  Hasil identifikasi jenis nematoda puru akar yang ditemukan adalah Meloidogyne javanica. Hasil penelitian menunjukan bahwa penggunaan P. fluorescens dapat mengendalikan serangan nematoda puru akar M. javanica pada tanaman tomat (S. lycopercisium). Pengaplikasian 40 ml P. fluorescens dengan kerapatan 109 cfu/ml pada tomat (S. lycopercisium) paling efektif dalam mengendalikan serangan nematoda puru akar (M. javanica) serta mampu meningkatkan pertumbuhan tanaman tomat.
EFEKTIVITAS EKSTRAK AKAR TAGETES (Tagetes sp.) UNTUK PENGENDALIAN NEMATODA PURU AKAR (Meloidogyne spp.) Isna Kartika Wati; Bambang Tri Rahardjo; Hagus Tarno
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 8 No. 1 (2020)
Publisher : Universitas Brawijaya

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Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak akar tagetes dalam daya hambat tetas telur dan mortalitas juvenil II Meloidogyne spp. serta memperoleh nilai Median Lethal Concentration (LC50) dan Median Lethal Time (LT50) ekstrak akar tagetes. Penelitian dilaksanakan pada Februari sampai dengan Juni 2015 di laboratorium Virologi Jurusan Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya Malang. Pengujian Ekstrak Akar Tagetes pada Meloidogyne spp. menggunakan rancangan acak lengkap (RAL) dengan 5 perlakuan yang terdiri dari konsentrasi 0 (kontrol), 5 %, 10 %, 15 % dan 20 % dengan pengujian pada telur dan Juvenil II Meloidogyne spp. Masing-masing perlakuan diulang sebanyak 4 kali. Data dianalisis menggunakan analisis ragam. Apabila terdapat perbedaan nyata pada setiap perlakuan maka dilanjutkan dengan uji beda nyata terkecil taraf 5%. Hasil penelitian menunjukkan bahwa pemberian ekstrak akar tagetes konsentrasi 20% mampu mempengaruhi daya tetas telur Meloidogyne spp. dengan persentase 83,07% dalam waktu 11 hari setelah aplikasi serta mortalitas juvenil II dengan persentase mortalitas sebesar 98,81% dalam waktu 24 jam. Nilai LC50 pada telur sebesar 1,396% dalam waktu 11 hari dan LC50 pada juvenil II Meloidogyne spp. sebesar 8,474% dalam waktu 3 jam. Sedangkan nilai LT50 pada juvenil II Meloidogyne spp. adalah 4,453 jam.
The Effect of Papaya Leaf Extract (Carica papaya L.) on the Mortality Rate of Spodoptera litura Fabricius Larvae and the Level of Damage to Soybean Leaves in Malang, Indonesia: A Greenhouse Simulation Sofia Ery Rahayu; Amin Setyo Leksono; Zulfaidah Penata Gama; Hagus Tarno
AGRIVITA, Journal of Agricultural Science Vol 45, No 1 (2023)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v45i1.3745

Abstract

Spodoptera litura Fabricius pest control using botanical pesticide from papaya leaves extract (PLE) is an alternative to substitute chemical pesticides. The study aimed to determine the levels of PLE's activity and evaluate the effectiveness of PLE from different altitudes (low and middle lands) and cultivars (Thailand and Indonesian) against the attack of S. litura Fabricius larvae. An experimental study with a randomized block design (n=5) was conducted from December 2020 until April 2021. Each replication used 100 soybean plants and sprayed with 0% or 20% PLE of Indonesian purple cultivar low land (UR-20%), Indonesian purple cultivar middle land (US-20%), Thailand cultivar low land (TR-20%), and Thailand cultivar middle land (TS-20%), respectively and observed at 72 hours after treatment. The results demonstrate that the middle lands contained active compounds slightly higher than the lowlands. The damaged soybean leaves and intensity of soybean leaf damage are highest in control (77% and 63%) and significantly different from the treatment group, 53-60% and 29-41%, respectively. The mortality of S. litura Fabricius larvae in the control group was 11%, and in the treatment group, 47-63%. PLE from different altitudes and cultivars reduce the intensity of soybean leaf damage by S. litura Fabricius larvae.
In Silico Study of Eugenol and trans-Caryophyllene also Clove Oil Fumigant Toxicity on Tribolium castaneum: In Silico Study and Fumigant Toxicity of Clove Oil Silvi Ikawati; Toto Himawan; Abdul Latief Abadi; Hagus Tarno; Alvan Fajarudin
Journal of Tropical Life Science Vol. 12 No. 3 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.03.07

Abstract

Alternative storage pest control that is more environmentally friendly than the use of synthetic chemical pesticides is to use botanical pesticides from plant essential oils, including clove (Syzygium aromaticum) which contains the main compounds eugenol and trans-caryophyllene. To study the various mechanisms of action of essential oils as botanical insecticides could use in silico approach through molecular docking. This study aims to predict the dominant binding mode(s) of a ligand with a protein of a known three-dimensional structure through docking. Then tested its fumigant activity on Tribolium castaneum. The docking results showed that the trans-caryophyllene and eugenol compounds had a more stable bond strength in the acetylcholinesterase enzyme T. castaneum than the control compound linalool. In addition, there is a synergy between eugenol and trans-caryophyllene when the two compounds interact with acetylcholinesterase. These results can be used as prediction material that trans-caryophyllene and eugenol have potential as protein acetylcholinesterase inhibitors of T. castaneum. After being tested in the laboratory, clove oil which contains two main compounds namely eugenol and trans-caryophylene has the potential to control T. castaneum with an LC50 value of 5,227 μL/L air. Keywords: Botanical insecticide, Caryophyllene, Docking, Eugenol, Fumigant,Syzygium aromaticum
The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae) Presti Mardiyani Purwaningtyas; Bambang Tri Rahardjo; Hagus Tarno
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5067

Abstract

Parasitic Root-Lession nematode of Pratylenchus coffeae can reduce the Indonesian coffee plants productivity. Several studies reported that Pseudomonas fluorescens and Bacillus subtilis endophytic bacteria were antagonistic bacteria to nematode. The objective of this research was to reveal the effectiveness of bacterial colonies density of P. fluorescens (UB_Pf1), B.subtilis (UB BS1), and a combination of both bacteria on nematode mortality using median lethal concentration (LC50) and median lethal time 50 (LT50). The densities of bacteria used in this study were 107, 109, 1011 and 1013 cfu/ml. 35 testing nematodes were used and the mortality was counted at 6, 12, 24, 36, and 48 hours after treatments. The results showed that LC50 values of P. fluorescens was (UB_Pf1) was 4,3x108 cfu/ml, LC50 B. subtilis (UB_Bs1) was 1,9x109cfu/ ml, and LC50 combination of both bacteria was, 8x107 cfu/ml. It implies that the application of the combination of both bacteria are more pathogenic than single bacterial treatment. The results also showed that the highest LT50 value was 13.21  hours combination of bacterial colonies with a density of 1013 cfu/ml and the lowest LT50 value was 52.00 hours on P. fluorescens (UB_Pf1) treatment with colonies density of 107 cfu/ml.How to CitePurwaningtyas, P. M., Rahardjo, B. T., Tarno, H. (2016). The Effect of Bacteria Colony Pseudomonas fluorescens (UB_Pf1) and Bacillus subtilis (UB_Bs1) on the Mortality of Pratylenchus coffeae (Tylenchida: Pratylenchidae). Biosaintifika: Journal of Biology Biology Education, 8(3), 286-293. 
Keanekaragaman dan komposisi spesies laba-laba predator dan parasitoid Hymenoptera pada tanaman jagung dengan dan tanpa refugia pada musim yang berbeda: Diversity and species composition of predatory spiders and Hymenopteran parasitoid on maize fields with and without refugia in different seasons Sulthoni, Fahmi; Tarno, Hagus; Rizali, Akhmad; Priawandiputra, Windra; Buchori, Damayanti; Johannis, Midzon
Jurnal Entomologi Indonesia Vol 20 No 3 (2023): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.3.258

Abstract

Refugia are plants that play an important role in the conservation of natural enemies in agroecosystem. Refugia plants are useful for providing shelter and host/prey for natural enemies, especially parasitoids and predators. This study was aimed to investigate the effect of refugia and different planting seasons on the diversity and species composition of natural enemies (especially predatory spiders and Hymenoptera parasitoids) in maize fields. Field research was conducted in two different seasons i.e., dry season (April to July 2022) and rainy season (September to December 2022) in Tumpang Village, Malang District. The maize fields for the study were grouped into four different areas, with two observation plots (size 40 m í— 50 m) in each area, consisting of a monoculture maize field (control plot) and a maize field with refugia plants (treatment plot). Sampling was conducted using pitfall traps (for predatory spiders) and yellow traps (for Hymenoptera parasitoids). Traps were set for 1 í— 24 hours and conducted at 2, 4, 6, 8, 10 and 12 weeks after planting. The results from two planting seasons were obtained predatory spider diversity amounted to 626 individuals consisting of 7 families, and 40 morphospecies, while Hymenoptera parasitoids amounted to 787 individuals consisting of 5 families, and 63 morphospecies. Based on generalized linear models analysis, it was found that refugia planting did not affect the diversity of natural enemies, but affected the species composition of predatory spiders. Different planting seasons affect the diversity and species composition of predatory spiders as well as Hymenoptera parasitoids.
OPTIMALISASI BUDIDAYA TANAMAN TEBU (SACCHARUM OFFICINARUM. L) DI LAHAN KERING BERBASIS VARIETAS DAN PERBANYAKAN BIBIT BERORIENTASI HAMPARAN, MEKANISASI DAN KEBIJAKAN Sudiarso, Sudiarso; Budi, Setyo; Tarno, Hagus; Sari, Sasmita
CAKRAWALA Vol 10, No 1: Juni 2016
Publisher : Badan Penelitian dan Pengembangan Provinsi Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6099.022 KB) | DOI: 10.32781/cakrawala.v10i1.53

Abstract

Penelitian ini berfokus pada optimalisasi budidaya tanaman Tebu (Saccahrum Officinarum, L) di lahan kering berbasis varietas dan perbanyakan bibit berorientasi hamparan dan mekanisasi serta kebijakan.Penelitian ini dilaksanakan di Kebun Wotansari Kabupaten Gresik dari bulan Oktober 2014 sampai Desember 2015. Penelitian menggunakan Rancangan Acak Kelompok Faktorial dengan dua faktor. Faktor I. Varietas terdiri 4 yaitu : Bululawang, VMC 76-16, Cokro, klon Columbia 2. Faktor II. Perbanyakan bibit terdiri 2 yaitu : Bagal dan Budchips. Masing-masing kombinasi perlakuan diulang 3 kali. Kombinasi perlakuan ada 24. Hasil penelitian : 1). Perlakuan varietas dan perbanyakan bibit menyebabkan interaksi nyata terhadap tinggi batang tebu waktu tanaman umur 3 bulan. Batang tebu tertinggi 361,7 Cm waktu umur 12 bulan dihasilkan varietas Bululawang dari bibit budchips yang ditanam secara double planting dan tidak berbeda nyata dengan klon Columbia 2. 2). Perlakuan varietas dan perbanyakan bibit menyebabkan interaksi nyata terhadap jumlah batang waktu umur 3 dan 6 bulan. Jumlah batang terbanyak 121 batang waktu umur 6 bulan dihasilkan varietas Cokro dari bibit bagal maupun budchips yang ditanam secara double planting dan tidak berbeda nyata dengan klon Columbia 2. Jumlah batang /10 meter terbanyak 129 batang waktu umur 12 bulan dihasilkan varietas Cokro dari bibit budchips dan tidak berbeda nyata dengan varietas Columbia 2 yang ditanam secara double planting.3). Waktu umur 12 bulan, varietas dan perbanyakan bibit tidak berpengaruh nyata terhadap brix batang tanaman kecuali perlakuan perbanyakan bibit waktu umur 9 bulan yang ditanam secara double planting. Waktu umur 12 bulan, brix batang tertinggi 25,4 % dihasilkan varietas Cokro dari bibit budchips dan tidak berbeda nyata dengan klon Columbia 2 yang ditanam secara double planting. 4). Perlakuan varietas dan perbanyakan bibit waktu umur 12 bulan secara analisa ragam tidak menunjukaan perbedaan nyata terhadap diameter batang tebu. 5). Waktu umur 12 bulan hanya perlakuan perbanyakan bibit yang berpengaruh terhadap rata-rata rendemen batang tebu. Rendemen tertinggi 11,2 % dihasilkan varietas Cokro dari bibit budchips yang secara analisa ragam tidak berbeda nyata dengan varietas lain yang ditanam secara double planting. 6). Waktu umur 12 bulan hanya perlakuan varietas yang berpengaruh pada berat tebu. Berat tebu per batang tertinggi adalah 2,1 kg dihasilkan varietas Cokro dari bibit budchips yang ditanam secara double planting.
Fungal Endophytic Beauveria bassiana in Chinese Kale Against Plutella xylostella (L.) Larvae Afandhi, Aminudin; Ikawati, Silvi; Baroro, Ismatul; Afiyanti, Mufidah; Sari, Rose Novita; Handoko, Handoko; Suyono, Tri; Setiawan, Yogo; Tarno, Hagus
Research Journal of Life Science Vol 8, No 3 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.03.4

Abstract

This study evaluated three methods of inoculation of Beauveria bassiana endophytes from Chinese kale and then infected into Plutella xylostella L to determine the mortality rate. The endophytic B. bassiana using three different inoculation methods including seeds-soaking, leaf-spraying, and soil-wetting. The Chinese kale seeds from Winsa variety, B.bassiana isolates derived from collection planthopper. The endophytic fungus was identified to be B. bassiana based on the analysis of colony morphology. The results of this study included the degree of colonization of Chinese kale plants and the mortality of P. xylostella. The average B. bassiana colonies in leaves were 13.89%, stems 2.77% and roots 5.55%. The results showed on leaves that the seeds-soaking method obtained higher colonization of B. bassiana rate when compared to leaf-spraying and soil-wetting. The highest percentage of P. xylostella mortality was generated from seeds-soaking (45%), soil-wetting (37.5%), leaf-spraying (35%). The B. bassiana fungus which caused the fastest LT50 with a seeds-soaking (409.48 hours), and then soil-wetting (679.15 hours), leaf-spraying (1090.21 hours). The positive effects of seeds-soaking of endophytic B. bassiana and mortality of Plutella xylostell.
Colony Growth, Sporulation, and Viability of Entomopathogenic Fungus Beauveria bassiana (Balsamo) Vuillemin on Various Agricultural Waste as Growing Media Kurniawan, Irvan; Afandhi, Aminudin; Tarno, Hagus
Research Journal of Life Science Vol 9, No 2 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.02.4

Abstract

Beauveria bassiana is an entomopathogenic fungus that is widely produced for the benefit of biological control agents of various insect pests. The current production that is expected is to use cheap media but produce quality isolates. This study aims to determine colony growth, sporulation and viability of Beauveria bassiana conidia in several agricultural waste media. The experiment was compiled in a complete randomized design and replicated four times. B. bassiana in rice bran media added with cricket flour  had the fastest colony growth while B. bassiana in rice husk media was the lowest, lower than the control treatment. In  wheat bran media added cricket flour has slower colony growth than rice bran with cricket flour media similar to control treatment but has the second highest sporulation after rice bran with cricket flour media. The highest viability belongs to B. bassiana which is grown on rice bran with cricket flour media. The production in each treatment medium has a real effect but the addition of cricket flour has a significant influence in the production of quality isolates.
Association between Predatory Arthropods and Weeds on Sugarcane Plants Devi, Mia Prastika; Rahardjo, Bambang Tri; Tarno, Hagus
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.4

Abstract

While a large number of farmers in the tropics consider weeds to be just a nuisance, some weeds can potentially have benefits for cultivated crops. Weeds around cultivated plants can act as a biocide, soil improvement, and a food source for humans and animals, as well as a habitat for some insects. Based on the regression analysist, weed density has a relationship to the diversity of predatory arthropod species (R2= 0.026; P<0.001) and the abundance of individual predatory arthropods (R2= 0.010; P<0.001). Beneficial weeds around sugarcane plantations create mutually beneficial interactions. Weeds can be used as a place to live, reproduce, and produce nutrients for predatory arthropods. Research on the role of weeds against natural enemies from predators in sugarcane agroecosystems is also rarely studied. In this study, the focus was on the association of weeds with the diversity and abundance of predatory arthropods in the sugarcane agroecosystem.
Co-Authors Abdul Latief Abadi Adriani Sunuddin Afiyanti, Mufidah Agus Nugroho, Agus Ainul Hayat Aji Santoso Aji Santoso Akhmad Rizali Alvan Fajarudin Amin Setyo Leksono Aminudin Afandhi Aminudin Afandhi Aminudin Afandhi Andik Isdianto Anggraeni, Alifia Nurma Annike Putri Damayanti Arifin Noor Sugiharto Arin, Iftita Yustitia Arum Yuli Kristanti Bambang Semedi Bambang Tri Rahardjo Bambang Tri Rahardjo Baroro, Ismatul Budi, Setyo DAMAYANTI BUCHORI Dede Durahman Dede Durahman Devi, Mia Prastika Dewi, Citra Satrya Utama Dwi Wulandari Edson Begliomini Eko Wahyu Budi Darmawan Eko Wahyu Budi Darmawan Estri Pamungkasih Fadhila Hasna Fauziah, Septia Hana Fery Abdul Choliq Firdausi, Wita Gatot Ciptadi Gatot Mudjiono Gunawan Prayitno Hamdoen, Fadel Muhammad Handoko Handoko Hari Sutrisno Hari Sutrisno Hasan Suprapto Hauroinsiyah, Fatimah Havinda Anggrilika W.S. Herandarudewi, Sekar Mira Hermawan, Romy Hongye Qi Irvan Kurniawan Isna Kartika Wati Istiqomah, Rina Nur Johannis, Midzon Kafif Andani Karisma Aditya Wardani Karuniawan Puji Wicaksono Karuniawan Puji Wicaksono Karuniawan Puji Wicaksono Karuniawan, Anggik Kazuyoshi Futai Liza Afifah Liza Afifah Liza Afifah Luqman Qurata Aini Manna, Dorkas Wantiawati Maria Ulfa Maris Purnanto Maris Purnanto Masahide Kobayashi Maspupah Huzni Maspupah Huzni Meinardhy, Ricky Mintarto Martosudiro Moch. Dawam Maghfoer Mudji Santosa Muhammad Anton Astoni Muhammad Febriansyah Nadiah, Annisrien Octavia, Evy Presti Mardiyani Purwaningtyas Presti Mardiyani Purwaningtyas, Presti Mardiyani Primastya Dinarwika Primastya Dinarwika Rahmadina Fitria Ristanti Ramayanti, Kinta Retno Dyah Puspitarini Ria Febrianasari Ria Febrianasari Rina Rachmawati Rizki Puji Widiastuti Rizki Puji Widiastuti Ronny Pamuji Ronny Pamuji Ryoko Hirata Sari, Riya Fatma Sari, Rose Novita Sari, Sasmita Satoshi Ito Seca Gandaseca Senoaji, Wasis Silvi Ikawati Silvi Ikawati, Silvi Slamet Wahyudi Sofia Ery Rahayu Sri Heriza Sri Heriza Sri Wahyuni Sudiarso Sudiarso Sukandar Sukandar Sulthoni, Fahmi Suputa Suputa Suyono, Tri Syamsulhadi, Mochammad Theresia Rani Kartika Ayu Toto Himawan Toto Himawan Toto Himawan Tsuyoshi Kajisa Wahyudie, Diyan Eko Wasiska Iyati Wiadnya , Dewa Gede Raka Winarno, Dinariningrum Rahma WINDRA PRIAWANDIPUTRA, WINDRA Yahya, Minhajul Qowim Yasa Palaguna Umar Yasushi Mitsuda Yogo Setiawan Zeni Ningrum Zeni Ningrum Zulfaidah Penata Gama