Article Per Year (5 Year)
p-Index From 2021 - 2026
1.532
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Tropical Medicine Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Vektora : Jurnal Vektor dan Reservoir Penyakit Jurnal Vektor Penyakit Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Journal of Dentistry Indonesia Indonesian Journal of Biotechnology Pharmacon Jurnal Dunia Kesmas Jurnal Abdi Insani JURPIKAT (Jurnal Pengabdian Kepada Masyarakat) Makara Journal of Science Berita Kedokteran Masyarakat Dharma: Jurnal Pengabdian Masyarakat Academic Hospital Journal Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Tri Wibawa
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

Published : 34 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Pharmacon

 1 
Pengaruh Perbedaan Metode Ekstraksi Metabolit Sekunder Streptomyces sp. GMR22 terhadap Toksisitas pada Sel BHK-21 Mentari, Diani; Naima, Mirtani; Wulansari, Riska; Widada, Jaka; Nuringtyas, Tri Rini; Wibawa, Tri; Wijayanti, Nastiti
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 1 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i1.8032

Abstract

Streptomyces sp. GMR22 is local isolate from Wanagama 1 Forest in Yogyakarta. They have the potential to be developed to produce active compounds because have PKS and NRPS genes.The active compounds from isolation are strongly influenced by various factors, one of them is extraction techniques. Effect difference of extraction technique will be affected by the quality of secondary metabolites produced.The purpose of this study was to compare the cytotoxicity effects of secondary metabolites of Streptomyces sp. GMR22 which have extracted with different stages from previous studies. The extraction technique was carried out by multilevel separatory funnel extraction methods, which was first extracted using non-polar solvent (n-hexane) and then extracted using semi-polar solvent (ethyl acetate). This research is important because in previous studies (separatory funnel only extracted using ethyl acetate) with the use of the lowest concentration in the dengue virus antiviral test (further test) caused 100% of deaths in BHK-21 cells.This study indicate that multilevel extraction result in lower CC50 value than previous studies. There are 49.160 µl/ml (n-hexane extract) and 284.56 µl/ml (ethyl acetate extract) while water extract is 464,38 µl/ml. FTIR compound analysis show that the three extracts produced have different spectrum patterns, especially in the n-hexane and ethyl acetate extract. Value of CC50 is not too high, it is expected that the secondary metabolites contained in the extracts can be used for further analysis such as antiviral testing because it is safe for normal host cells such as BHK-21 cells
Pengaruh Perbedaan Metode Ekstraksi Metabolit Sekunder Streptomyces sp. GMR22 terhadap Toksisitas pada Sel BHK-21 Diani Mentari; Mirtani Naima; Riska Wulansari; Jaka Widada; Tri Rini Nuringtyas; Tri Wibawa; Nastiti Wijayanti
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 1 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i1.8032

Abstract

Streptomyces sp. GMR22 is local isolate from Wanagama 1 Forest in Yogyakarta. They have the potential to be developed to produce active compounds because have PKS and NRPS genes.The active compounds from isolation are strongly influenced by various factors, one of them is extraction techniques. Effect difference of extraction technique will be affected by the quality of secondary metabolites produced.The purpose of this study was to compare the cytotoxicity effects of secondary metabolites of Streptomyces sp. GMR22 which have extracted with different stages from previous studies. The extraction technique was carried out by multilevel separatory funnel extraction methods, which was first extracted using non-polar solvent (n-hexane) and then extracted using semi-polar solvent (ethyl acetate). This research is important because in previous studies (separatory funnel only extracted using ethyl acetate) with the use of the lowest concentration in the dengue virus antiviral test (further test) caused 100% of deaths in BHK-21 cells.This study indicate that multilevel extraction result in lower CC50 value than previous studies. There are 49.160 µl/ml (n-hexane extract) and 284.56 µl/ml (ethyl acetate extract) while water extract is 464,38 µl/ml. FTIR compound analysis show that the three extracts produced have different spectrum patterns, especially in the n-hexane and ethyl acetate extract. Value of CC50 is not too high, it is expected that the secondary metabolites contained in the extracts can be used for further analysis such as antiviral testing because it is safe for normal host cells such as BHK-21 cells
Co-Authors Abdul Rahman Siregar Abdul Rahman Siregar, Abdul Rahman Abu Tholib Aman Adi Sofyan Ansori, Muhammad Ai Hertati, Ai Ali Wardana Anika Prastyowati, Anika Apon Zaenal Mustopa Arief Mulyono Arifah Khusnuryani Aris Haryanto Aris Haryanto Arum Sih Joharina Boy Bachtiar Cita Rosita Sigit Prakoeswa Daniwijaya, M Edwin Widyanto Diani Mentari Diptyanusa, Ajib Domas Fitria Widyasari Erni Martani Farahannisaa, Kintan Adelia Farida Dwi Handayani Farida J. Rachmawaty Farida J. Rachmawaty, Farida J. Febe, Ester Firdaus, Moh Egy Rahman Frutos, Roger Gunawan Gunawan Gunawan Madyono Putro Hakim, Mohamad Saifudin Hamidah, Berliana Hardyanto Soebono Haryanto, Darban Hayani Anastasia Hera Nirwati Huda Shalahudin Darusman I Kadek Mulyawan Ikramullah, Muh. Chaeril Intan Berlianty Irawan, Shasmita JAKA WIDADA Laksmi Wulandari Maguin, Sylvie Mahardika Agus Wijayanti Marsetyawan H.N.E. Soesatyo Marsetyawan H.N.E. Soesatyo, Marsetyawan H.N.E. Mirtani Naima Mubasysyir Hasanbasri Mulyawan, I Kadek Munawir Sazali Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ning Rintiswati Ning Rintiswati Novriana, Riska Nurhaida Widiani Ova Emilia Pakpahan, Cennikon Pascawati, Nur Alvira Puteri, Rr. Astrid Aulia Artiono R.C. Hidayat Soesilohadi Rania Ayu Aziza Ratna, Lita Tri Rifqiyah Nur Umami, Rifqiyah Nur Riska Wulansari Ristiyanto Ristiyanto Roger Frutos S Siswanto S. Sutaryo Sadi Setyawan Budiharta Supargiono Supargiono Suratna, Suratna Sutaryo1 S, Sutaryo1 Sylvie Maguin Tri Baskoro Tunggul Satoto Tri Rini Nuringtyas Utomo, Humam Santosa Wardana, Ali Widya Asmara Wirastuti, Fita Wulansari, Riska Yati Soenarto Yundari, Yundari