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Journal : Berita Kedokteran Masyarakat

Accuracy of the Kato-Katz and direct examination in the diagnosis of worms in preserved feces Gunasari, Lala Foresta Valentine; Triana, Dessy; Reyudo Suringgar, Sadid
BKM Public Health and Community Medicine PHS8 Accepted Abstracts
Publisher : Universitas Gadjah Mada

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Abstract

Purposes: Soil-transmitted helminth (STH) causes helminthiasis with an incidence of 24% in the world's population. Children aged 2-14 years are the population most susceptible to helminthiasis. In Indonesia, there are 60.4 million children who have worms. Worms can cause delays in the growth and development of children. Worms can be diagnosed through a qualitative (direct examination) and quantitative (Kato-Katz) stool examination. This study aimed to analyze the accuracy of stool examination results using the Kato-Katz method and the Direct Examination method on 10% formalin-preserved feces. Methods: The research design used an observational study with diagnostic tests. The research subject amounted to 140 samples of 10% formalin preserved feces for 13 months. Examination of preserved stool specimens used the Kato-Katz and Direct Examination methods and was observed using a light microscope. Results: The results showed that 45,71% were infected with STH using the Kato-Katz method and direct examination of 35.71%. The accuracy test showed that the sensitivity and specificity values of the Kato-Katz method were 86.79% and 79.31%, respectively. The direct examination was obtained by 64.15% and 81.60%. Conclusions:The Kato-Katz method is more sensitive than the direct examination method in diagnosing helminthiasis in preserved feces, but the direct examination method has a higher specificity value than the Kato-Katz method.
Accuracy test of the Kato-Katz and direct examination method for diagnosing helminthiasis using preserved stool Gunasari, Lala Foresta Valentine; Triana, Dessy; Suringgar, Sadid Reyudo
BKM Public Health and Community Medicine Vol 38 No 01 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bkm.v38i01.3535

Abstract

Purposes: More than 1.5 billion people, or a quarter of the world's popula-:on, are infected by soil-transmi>ed helminth (STH). Children aged 2-14 years are the most suscep:ble to helminthiasis. In Indonesia, there are 60.4 million children infected with STH, with an average prevalence rate of 31.8%. Helminthiasis can cause growth and development disorders in children. The diagnosis of helminthiasis can be made by examining stool using qualitative (direct examination) and quantitative (Kato-Katz) methods. This study aimed to determine the accuracy of both methods in diagnosing helminthiasis using preserved stool specimens. Methods: This research design was an observational study with a cross-sec-:onal approach. A total of 140 stool samples preserved using 10% formalin for 13 months were examined with the Kato-Katz and direct examination methods using a light microscope. Results: Among the 140 stool samples examined, 64 samples (45.71%) were found positive for STH using the Kato-Katz method, while with the direct examination method, 50 samples (35.71%) were positive for STH. The accuracy test showed that the sensitivity and specificity values of the Kato Katz method were 86.79% and 79.31%, while the direct examination method was 64.15% and 81.60%, respectively. Conclusions: The Kato-Katz method was more sensitive than the direct examination method in diagnosing helminthiasis in preserved stool, but the direct examination method had a higher specificity value than the Kato-Katz method. In addition, the accuracy of examination of fresh and preserved stool specimens in diagnosing helminthiasis was not significantly different.