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All Journal International Journal of Evaluation and Research in Education (IJERE) Prosiding Seminar Biologi Jurnal Pendidikan Sains Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Islam and Muslim Societies Indonesian Journal of Urology Jurnal Bioterdidik: Wahana Ekspresi Ilmiah Bioedukasi: Jurnal Pendidikan Biologi Jurnal Pendidikan Biologi Indonesia Biology, Medicine, & Natural Product Chemistry Jurnal Florea UNEJ e-Proceeding Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan BIOEDUKASI Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Proceeding Biology Education Conference Berkala Penelitian Hayati Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Ta'dib Jurnal Ilmu Hayat
Umie Lestari
Universitas Negeri Malang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Indonesian Journal of Urology

 1 
PRODUKSI ANTIBODI POLIKLONAL HASIL INDUKSI ISOLAT PROTEIN NON KINASE DARI MEMBRAN SPERMATOZOA MANUSIA Lestari, Umie; Purnomo, Basuki B
Indonesian Journal of Urology Vol 15 No 2 (2008)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v15i2.350

Abstract

Objective: The aim of this research is to get the profile of human sperms membrane protein based on identification for molecular weight, concentration protein, phosphorylation activity, and ability to raise enough antibodies. Material and methods: There are three phases in this research, first, isolation protein of human sperms membrane with using lysis buffer containing Tween-20, second, determination of molecular weight, phosphorylation activity, and protein concentration, and third, antibody production. Molecular weight of protein membrane was detected by SDS-PAGE, confirmation of protein concentration measured Biuret method, kinase activity measured using spectrophometry at its optimum condition, and then production of antibody that corfirmed by ELISA technique. Results: Human sperms contain the protein with molecular weight of 201, 163, 116, 97, 72, 56, 48, 32, 27, 20, and 17kDa. The highest phosphorylation activity was found on protein with molecular weight of 20 kDa is 30.197x 10-3 unit, while the lowest phosphorylation activity is 28.976x10-3 unit was shown on protein with 116 kDa of molecular weight and then   this protein can induce production of antibody. Biuret method show the protein concentration from protein with molecular weight 116 kDa is 410 ug/mL. Conclusion: The protein that has molecular weight of 116 kDa can be used as immunocontraception agent because phosphorylation activity has lower, good concentration, and could induce the production of antibody.
Co-Authors Abdul Gofur Abdul Gofur Abdul Gofur Abdul Gofur Abu Husen Abu Husen Afifah Nur Aini Alif Yanuar Zukmadini Aloysius Duran Corebima Amin, Ihya Fakhrurizal Anton Setia Budi Ardiyas Robi Saputra Basuki B Purnomo, Basuki B Beno Wahono Beno Wahono, Beno Betty Lukiati Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani, Cicilia Desy Putri Rahmawati Dewi Murni Dina Maulina Dina Maulina Dwi Anggorowati Rahayu Endang Suarsini Endik Deni Nugroho Fahmi, Muhammad Najib Farizha Irmawati Farizha Irmawati, Farizha Fitri, Nurul Hadi Suwono Haqqi Anajili Setyanto Haqqi Anajili Setyanto, Haqqi Anajili Herawati Susilo Ihya Fakhrurizal Amin Ika Oktaviana Ika Oktaviana, Ika Istamar Syamsuri, Istamar Istamar Syamsyuri Izza Rohman Kurniawan Setia Putra Listawati, Rizki Mei Misgiati, Misgiati Mochammad Ali Mashur Moh. Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohammad Amin Mohammad Amin Mohammad Amin Monalisa PJ Taihuttu Muh. Nur Akbar Murni Sapta Sari Nafisatuzzamrudah Nafisatuzzamrudah Nanda Earlia Nandang Mufti Naning Wahyuni Nindya Ulfa Wardhani Nugrahaningsih Nugrahaningsih Nur Hayati Rahman Fadli Rahmawati D Rahmawati Darussyamsu Reni Istiningrum Reni Istiningrum, Reni Ridho, Muhammad Andi Ali Ristiana, Evi Ritia Rahmawati Ritia Rahmawati, Ritia Rizka Elan Fadilah, Rizka Elan Rizki Mei Listawati Rizkia Suciati Rizkia Suciati Saparuddin Saparuddin Sembiring, Rinawati Siti Nur Arifah Siti Rahma Lestaluhu Sri Endah Indriwati Sri Rahayu Lestari Sulistyo Dwi Kartining Putro Sulistyo Dwi Kartining Putro, Sulistyo Dwi Kartining Susriyati Mahanal Sutiman B Sumitro Sutiman B Sumitro, Sutiman Sutiman B. Sumitro Ulin Nuha Ulin Nuha, Ulin Ulya Ruwaida Umi Fitriyati Urla Tri Wulanzani Urla Tri Wulanzani, Urla Tri