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STUDI LAJU PERKEMBANGAN BUAH KAKAO PASCA APLIKASI MEDIA JAMUR TIRAM (Pleurotus ostreatus (Jacq) P. Kumm) Roliana Roliana; Umrah Umrah; Miswan Miswan
Biocelebes Vol. 9 No. 2 (2015)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The study Cocoa Fruit rate Post Application Development Media Media Mushroom Compost Waste Oyster Mushroom (Pleurotusostreatus (Jacq) P. Kumm) This study was conducted in the village Langaleso, in order to determine the effect of dose and post-application of compost media oyster mushroom (Pleurotusostreatus( Jacq) P. Kumm) on the rate of development of the fruit and seed weight of cocoa. The research was designed in completely randomized design (CRD), consisting of seven treatments and three replications. The composition of the treatment of waste compost fertilizer applications on any media oyster mushroom cocoa plant P (control, without fertilizer application), P1 (application of compost 500 g), P2 (application of compost in 1000 g), P3 (compost application 1500g), P4 (compost application 2000 g), P5 (compost application 2500 g), P6 (3000 g of cocoa compost applications). Parameter observations pace of development of the cocoa plant fruit and cocoa bean weight. The rate of development of cocoa pods showed between fruit length and circumference of fruit on a day to Fifty. Results of the study showed weight cocoa beans treated (P5) is the best treatment in terms of the average weight of the cocoa beans 45.81 g.Keywords : Cocoa Beans, Compost Waste Oyster Mushroom Media, (Pleurotus ostreatus (Jacq) P.Kumm)
PENGARUH PEMBERIAN BIOKOMPOS (BAHAN AKTIF Trichoderma sp., FORMULA SEDIAAN TABLET) TERHADAP PERTUMBUHAN BIBIT KAKAO (Theobroma cacao L.) Umrah Umrah; Firji Della Sugeha; Miswan Miswan
Biocelebes Vol. 9 No. 2 (2015)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This research were performed to find out the effect of bio-compost treatment (Trichoderma sp. active material, formula of tablet-form) towards the cacao seedling’s growth.This research has been conducted between June-August 2014, at Langaleso Village, Kecamatan Dolo, districtof Sigi, Provinsi of Central Sulawesi. This research was designed in form of six treatments and threereplication according to Completely Randomized Design.The design of treatments is mixture of soil and bio-compost Trichoderma sp. active material formula of tablet-form, which P0 (soil without bio-compost as control), P1 (mixture of soil and bio-compost with 5 tablets per kg substrate Trichoderma sp. active material), P2 (10 tablets per kg substrate), P3 (15 tablets per kg substrate), P4 (20 tablets per kg substrate), and P5 (25 tabletsper kg substrate). The Parameter observed includes plant hight (cm) and number leafs (sheet) that observed in each week during six weeks, biomass plant dryweight (g) that observed in sixth week. The result of reseach showed that bio-compost treatment (Trichoderma sp. Active material formula tablet-form) indicate significant effect to cacao seedling. The conclusion is P5 treatment are the best among the other treatments including control, according to the growth rate wereon plant height (22,40 cm), number of leafs (9,67) and biomass plant dryweight (2,12 g).Keywords : cacao seedling, bio-compost, Trichoderma sp.
PENGARUH PEMBERIAN LIMBAH MEDIA JAMUR TIRAM (Pleurotus ostreatus (Jacq)P.Kumm) TERHADAP PERTUMBUHAN BIBIT KAKAO (Theobroma cacao L.) Setia Ayu Lestari; Umrah Umrah; Miswan Miswan
Biocelebes Vol. 10 No. 2 (2016)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The  growth of cocoa plant seedlings (Theobroma cacao L.) can be Increased by using organic material, for examples oyster mushroom media waste (Pleurotus ostreatus (Jacq)P.Kumm). The purpose of this study was to determine the effect of oyster mushroom media waste on the growth of cocoa plant seedlings. This study was conducted during period of March until May 2015. Cocoa plant seeds was planted in plastic bags with size 30 x 40 cm, designed in a Completely Randomized Design (CRD). The treatment consists of P0 (soil media without oyster mushroom media waste, as a negative control);P1 (soil media is 90% + oyster mushroom media waste is 10%), P2 (soil media is 80%+ oyster mushroom media waste 20%), P3 (soil media is 70% + oyster mushroom media waste 30%), P4 (soil media is 60% + oyster mushroom media waste is 40%), P5 (soil media is 50% + oyster mushroom media waste 50%), P6 (soil media is 3 kg + NPK Fertilizer is 3 g, as a positive control).The observation parameters includes the plants high, number of leaves, stem diameters, plants fresh biomass and plants dry biomass. The results was showed that the best tretment is P2 acording there are average parameters,  include 24,63 cm high of plant; 10,66 number of leaves; 0,42 cm stem diameters; 4,73 grams of fresh biomass and 2,15 grams of dry biomass.                      Keywords : Cocoaseedlings, oyster mushroom media waste (Pleurotus ostreatus (Jacq)  P.Kumm), Theobroma cacao L.
UJIVIABILTAS INOKULUM JAMUR TIRAM PUTIH ( PLEUROTUS OSTREATUS ( JACQ) P. KUMM) DALAM BENTUK SEDIAAN CAIR Siti Fatmawati; Umrah Umrah; I Nengah Suwastika
Biocelebes Vol. 11 No. 1 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The research aimed to know the best formulations of basic substrate inoculums  white oyster mushrooms (Pleurotus ostreatus (Jacq) P. Kumm) in the best liquid forms and their viability on production media. The research was conducted at Laboratory  Biotekchnology of Depertement of Biology Faculty of Matematics and Natural Sciences, starting from March until July 2015. The experimental designed used was a Completely Randomized Design (CRD) which consisted of seven treatment and three replications. The compositions of the treatment were the ratio among wood sawdust, rice brain, corn starch and calcium (g) in successive sequences as follows : P0 (0 : 50: 50 : 3); (P1 10 : 45 : 45 : 3); (P2 20 : 40 : 40 : 3); (P3 30 : 35 : 35 : 3); (P4 40 : 30 : 30 : 3); (P5 50 : 25 : 25 : 3); (P6 60 : 20 : 20 : 3). The planting media used  in this research were production media. The observation parameters namely the ability fungal  growth  of inoculums  that meets surface the media with viability a high. The result of the research showed that P6 was the best treatment among. In the viability of the inoculums grew well on media production surface with good viability at 8 days of incubation. 
Metode Inokulasi dan Pengamatan Perkembangan Phytophthora palmivora Serta Gejalanya Sebagai Penyebab Penyakit Busuk Buah Kakao (Theobroma cacao L.) Dian Sri Anugrah; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Phytopthora palmivora is one of pathogenic mushroom causes black pod   iisease of cocoa plant. The research about P. palmivora development and symptoms as the cause black pod   disease of cocoa (Theobroma cacao L.) was conducted from March until August 2016. Inoculation treatment of P. palmivora compared with aquades qontrols carried on cocoa fruit, with use “singlespot” and “polyspot” method. Observation parameters include incubation periode, extensive spotting, the percentage of P. palmivora infection and observation of macroscopic and microscopic P. palmivora mushroom. The results showed that in P. palmivora infection attack on cocoa fruit tends to grow up from third day to seventh day with average extensive spotting infection start on third day to seventh day are 6,4 cm, 8,1 cm, 9,6 cm, 12,72 cm, and 12,20 cm. Extensive spotting average P. palmivora infection most big is 14,22 cm while the percentage average most big is 90% and macroscopic structure showing their blackish brown spot on fruit surface and derived morphological characters of the hyphae are not insulated and ramified. Visible mycelium, clamydospores, papilla, zoospore dan sporangium forms such as Pier fruit on microscopic observation.
PERTUMBUHAN MISELIUM Trichoderma sp. PADA LIMBAH CAIR TEMPE DAN LIMBAH AIR KELAPA Juliana Juliana; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The purpose study was to determine potential tempe industry liquid waste and coconut water waste as medium propagation miselium Trichoderma sp. The treatment was conducted based on completely randomized design, consisting of seven treatments and three replication, namely: P0 (control), P1 (tempe industry liquid waste 100%), P2 (tempe industry liquid waste 80% + coconut water waste 20%), P3 (tempe industry liquid waste 60% + coconut water waste 40% ), P4 (tempe industry liquid waste 40% + 60% coconut water waste), P5 (tempe industry liquid waste 20% + 80% coconut water waste), P6 (100% coconut water waste). Variable observation included are media pH formula and the weight of mycelium biomass Trichoderma sp. The results showed that Trichoderma sp. Can be grown on all treatments except treatments P0 (control). The treatments produced in the highest mycelium biomass is P3 : 1595,333 mg per 100 mL medium, and this not significantly different from P2 : 1566,667 mg, but significantly different with all treatments.
Pengamatan Oncobasidium theobromae secara Makroskopis dan Mikroskopis, serta Gejala Serangan Sebagai penyebab Penyakit Vascular Streak Dieback (VSD) pada Tanaman Kakao di Kabupaten Sigi, Sulawesi Tengah Sitti Ardianti; Umrah Umrah; Asrul Asrul
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Oncobasidium theobromae are fungi that causes disease Vascular Streak Dieback (VSD) on the cocoa plants which can causes a totaly death, because the mechanism attack which systemic. The purpose of this study was to observe the morphology of O. theobromae macroscopically and microscopically, as well as symptoms of VSD attack on the cacao plants. This research was conducted from March to August 2016 in the Laboratory of Biotechnology Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University (FMIPA UNTAD). The result of macroscopic observation showed that O. theobromae have white mycelium on the early growth, then the color change to cream and texture like cotton. In microscopic observation, it has branched mycelium and irregular septate. VSD attack symptoms characterized with the leaf has necrosis, the former sitting leaf and the leaf base has three dots, twigs become  toothless and the ends of twigs become dry.
APLIKASI BIOKOMPOS “TRICHOSUBUR PROTECT” DAN PENGARUHNYA TERHADAP BERAT UMBI BAWANG MERAH (Allium ascalonicum L) VARIETAS LEMBAH PALU Nurzakia Nurzakia; Umrah Umrah; Abdul Rahim Thaha
Biocelebes Vol. 11 No. 2 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The research of the test of bio-compost effectiveness “Trichosubur Protect” to the red onion varieties growth (Allium ascalonicum L) in Palu valley has been conducted since from November 2015- to merch 2016. It was located in Hortus Botanicus land area (Botanical Park) departemen of Biology, Mathematics and Natural Sciences Faculty of Tadulako University. This research was designed through complete randomized design that consisted of 5 treatments and 3 repetitions. The treatmen was planting media (19 kg soil mix + 1 kg paddy husk) through bio-compost application “Trichosubur Protect” : P1 (planting media + 10 g “Trichosubur Protect”), P2 (planting media + 20 g “Trichosubur Protect”), P3 (planting media + 30 g “Trichosubur Protect”), P4 (planting media + 40 g “Trichosubur Protect”).The Parameters include the observation of leaf weight at harvest (g), the weight of tubers at harvest (g), root weight at harvest (g). The results showed that the application biokompos "Trichosubur Protect" effective (significant) to the weight of onion bulbs (Allium ascalonicum L) varieties Palu valley during harvest. P4 is the best treatment compared with other treatments, is seen in the average weight of leaves at harvest (7.21 g), the weight of tubers at harvest (18.73 g), root weight at harvest (0.87 g).
Formulasi Substrat Dasar Kotoran Sapi dan Limbah Cair Tempe dengan menggunakan Inokulum Rumen Sapi Untuk Studi Awal Produksi Biogas Bidayanti Ruru; Umrah Umrah; Orryani Lambui
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The research of “Biogas Production by Utilizing of Basic Substrate of  Fermented Liquid waste of tempeh and Cow Waste by Using Inoculum from Cow Rumen” for the basic study of biogas production was held on May until July 2016 at department Biology Laboratory unit Biotechnology, Faculty of Mathematic and Natural Sciences, University of Tadulako. The purposed of this research is to produce biogas by utilizing the basic of liguid waste of tempeh and cow waste, and also to know the concentration of using the rumen inoculum of cow rumen. This research is designed with completely randomized design which consists of six treatment with three repeating. the basic substance are composed of 500 grams of cow dung and 500 ml of liquid waste tempe.The composition of treatment is : ( P0 100% Basic Substrate Without inoculum),  ( P1 80% Basic Substrate + 20% inoculums) ,(P2 60% Basic Substrate + 40% inoculums), (P3 40% Basic Substrate + 60% inoculums) , (P3 20% Basic Substrate + 80% inoculums), (P5 100% inoculum Without basic substrate). Observation variable are consist of biogas volume measuring, pH measuring, light test and temperature measuring. anaerobic fermentation process is carried out in a culture bottle with a volume of 1000 ml bottle mouth top covered with a balloon for gas storage. The results of this research show that Biogas could be produced by using the basic substrate of fermented liguid waste of tempeh and cow waste with the inoculum from cow rumen, at, P2, P3, dan P4 treatment. The highest volume of gas in P2 treatment (60% Basic Substrate : 40% inoculum) which has 553,33 cm3. The pH which in the culture is about 6,33-6,46 and the result flame with temperature in 152ºC.
UJI KEEFEKTIFAN PUPUK BIOKOMPOS “ Trichosubur Protect “ TERHADAP PERTUMBUHAN BIBIT KAKAO ( Theobroma Cacao L ) Evisilvia Evisilvia; Umrah Umrah; Yusran Yusran
Biocelebes Vol. 12 No. 1 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research on the effectivenes of the test biokompos ’’ Trichosubur Protect’’on the growth of the cacao plant seeds have been conducted from November 2015 through march 2016 at the cacao gardens hortus Botanicus ( Botanical Garden ) department of Biology, Faculty of Mathematics and Natural Sciences Tadulako University, Palu- Central Sulawesi. The objective is to determine the dose biokompos “ Trichosubur Protect “ which is effective against the growth of cacao seedlings. The study was designed in a compososition of the treament as fllows : P0 ( Basal medium without “ Trichosubur Protect “),P1 ( Basic medium + ’’ Trichosubur Protect “ 10 g), P2 ( basic medium + “ Trichosubur Protect “ 20 g), P3 ( Basic medium + “ Trichosubur Protect “ 30 g), P4 ( Basic medium + “ Trichosubur Protect” 40 g), P5 ( medium Ground + NPK 2 g). Basic media used in the form of a mixture of soil 19 kg + 1 kg of rice husk, put in a polybag 25 x 30 cm 13 kg as the planting of cacao. Parameters include observavation every week; seedling height (cm), number of leaves ( leaf),the girth (mm).observation at harvest inculude: Biomassa (g),leaf weight (g),the weight rod (g), root weight(g),and root dry weight (g). The result shwed that treament P4 ( basic + “ Trichosubur Protect “ 40 g) the treatment is more efeective when seen from the results of last observatoins covering, the everage 29,07 cm plant height, leaf number 40 strands, stem diameter 3,73 cm, weight of leaves at harvest time 12,03 g ( Moisture in the leaves 44,30 g ),weight at harvest stalks of 7,62 g( moisture in the trunk 56,30 g), root weight at harvest 5,93g ( Moisture at the root of 92,80 g), the weight of biomassa when harvested 27,22 g, 4,42 g dry weight of leaf, stem dry weight of 1,98 g, 8,06 g dry weight biomassa.
Co-Authors A. St. Latifah Humairah A AA Sudharmawan, AA Adhiwijaya, Ardian Adi Putra, Andi Budiyanto Aisah Jamili Alwi Alwi Alwi Millang Amalia Purnamasari Zainal Amiruddin Kasim Andi Lindhemuthianingrum Annawaty Annawaty, Annawaty Asri Ismail Asri Pirade Paserang Asriani Hasanuddin Asrul Asrul Asrul Asrul Bidayanti Ruru Budi Indarsih, Budi Burhan, Kurnia Dana Sulistia Dewi, Ni Made Aryani Saraswati Dhini Arwindah Dian Sri Anugrah Djoko Kisworo Eka Fitriani Eny Yuniati Evisilvia Evisilvia Fahri Fahri Febiyanti Firdasari Firdasari Firji Della Sugeha Fitri Kurnianingsih Fitriani Fitriani Fitriani Husain Gina, Fina Alimatul Hardianti Lestari Hamid Hasriani Hasriani Humaero, Siti I Nengah Suwastika I Nyoman P. Aryantha Ika Merdeka Wati Ikha Wahyuni Ilham Bakri Abdul Rahim Thaha,Isrun, Ilham Bakri Irawan, Adit IRWAN LAKANI Jamaluddin Jamaluddin Jaya, I Nyoman Sukartha Jesica Pasaribu Juliana Juliana Kasman Sulaiman Khadijah Kirana Kirana Laksono Trisnantoro Lamadjido, Sri Rahayu Latifah Merdekawaty Lina Mahardiana Makmur, Baitul Mardiana Upara Maulana, Ardian Maulida, Nurhidayatul Meryany Ananda Miranty Sirappa Miswan Miswan Moh. Iqbal, Moh. Mufida Al Idrus Muh. Arsal N. Maddo Muhaemin Muhaemin Muhammad Alwi Muhammad Amiruddin Muhammad Saleh Muhammad Yusril Basir Murnawati Murnawati Musdalifa Musdalifa Musjaya M. Guli Mustafid Rasyiid Mutma Inna Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Naksabandi, Roydatul Robbi Ni Ketut Dewi Haryani, Ni Ketut Dewi Niluh Budi Artini Nisfaun Safitriana Nova Damayanti Novika Nur Asrinawaty Nur Hikma Nurfianti Nurhayati N Mangopo Nurvita Nurvita Nurzakia Nurzakia Orryani Lambui Rahayu Rahayu Rahmania Rahmania Rahmiati Rahmiati Ramadanil Pitopang Rifka Rifka Rizki Purnamasari Rizkita Rachmi Esyanti Roliana Roliana Saparudin Saparudin Septian, I Gede Nano Setia Ayu Lestari Sitti Ardianti Sitti Masyitah Sri Utami Subhan nuradzan Tamzil, Moh Hasil Tapaul Rozi Tjandra Anggraeni Tjandra Anggraini Tri Marsha Hasibuan Triana, Nina Risnanda Trismansyah Trismansyah Wahyu Harso Wahyu Hidayat Wasilahturahman Widiawati Yulia Astuti Yusran Yusran Yustilawati, Eva