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FORMULASI SUBSTRAT DASAR KOTORAN AYAM DAN LIMBAH CAIR TEMPE DENGAN INOKULUM RUMEN SAPI UNTUK STUDI AWAL PRODUKSI BIOGAS Nurhayati N Mangopo; Umrah Umrah; Kasman Kasman
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Production of biogas using the basic substrate of tempeh’s liquid waste and chicken’s waste with the bacteria inoculum of cow’s rumen has been experimentally conducted. This study aims to produce biogas using the substrate with the bacteria inoculum of cow’s rumen and know the optimal mixture ratio of the basic substrate and the inoculum. In this study, the amounts of basic substrate applied were 500 g chicken’s waste and 500 mL liquid waste of tempeh. The study was designed using Complete Random Design comprising 6 treatments with 3 times repetitions, respectively. The treatments tested were P0 (100% basic substrate), P1 (80% basic substrate + 20% inoculum), P2 (60% basic substrate + 40% inoculum), P3 (40% basic substrate + 60% inoculum), P4 (20% basic substrate + 80% inoculum), P5 (100% inoculum). These treatments were fermented with anaerob bacteria for 10 days in the fermentor 1000 mL mounted with the biogas collecting balloon. Variables observed in this study were the measurements of biogas volume, culture medium pH, and biogas burning temperature, and the testing of biogas flame colour. As result of observation, unlike P5, the examined treatments of P0, P1, P2, P3 and P4 could able to produce biogas with the respectively produced volume of 53,6 cm3, 56,7 cm3, 30,0 cm3, 90,0 cm3 and 73,3 cm3. While, the measured values of culture medium pH and biogas burning temperature were 6,23-6,63 and 152oC with the flame of red colour. This study shows that the optimal mixture ratio of the basic substrate and the inoculum for producing biogas is 2:3, and the produced biogas is potential to be used as an alternative energy. Keywords: Biogas, Chicken’s waste, Liquid waste of tempeh, Cow’s rumen
MONITORING KETAHANAN HIDUP SEMUT HITAM Dolichoderus thoracicus Smith PADA SARANG BUATAN DI TANAMAN KAKAO Murnawati Murnawati; Annawaty Annawaty; Umrah Umrah
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The Black Ant, Dolichoderus thoracicus Smith have long been recognized as a potential biological control agent in Cocoa plantation. The aims of this study were to determine how long D. thoracicus can survive on artificial nests in cacao plantation and to identify the ant competitor of D. thoracicus. The research conducted in three cocoa plantation i.e. Desa Labuan Panimba, Desa Langaleso, and Desa Pantoloan in Donggala District, Sulawesi Tengah. Artificial nest of D. thoracicus made from bamboo and palm leaves smeared with palm sugar solution and then hung in cocoa trees. The black ant, D. thoracicus can be survived for thirty days in the cacao plantation in Desa Labuan but only one day in Desa Langaleso. The weaver ant (Oecophylla smaragdina) and Philidris sp. were identified as the competitors of D. thoracicus ants inside the artificial nests. Keywords: Dolichoderus thoracicus, artificial nests and ant competitors.
FORMULASI SUBSTRAT DASAR KOTORAN KAMBING DAN LIMBAH CAIR TEMPE DENGAN INOKULUM RUMEN SAPI UNTUK STUDI AWAL PRODUKSI BIOGAS Dhini Arwindah; Umrah Umrah; Kasman Kasman
Biocelebes Vol. 12 No. 3 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Production of biogas using the basic substrate of tempeh’s liquid waste and goat’s waste with the bacteria inoculum of cow’s rumen has been experimentally conducted. This study aims to produce biogas using the substrate with the bacteria inoculum of cow’s rumen and know the optimal mixture ratio of the basic substrate and the inoculum. In this study, the amounts of basic substrate applied were 500 g chicken’s waste and 500 mL liquid waste of tempeh. The study was designed using Complete Random Design comprising 6 treatments with 3 times repetitions, respectively. The treatments tested were P0 (100% basic substrate), P1 (80% basic substrate + 20% inoculum), P2 (60% basic substrate + 40% inoculum), P3 (40% basic substrate + 60% inoculum), P4 (20% basic substrate + 80% inoculum), P5 (100% inoculum). These treatments were fermented with anaerob bacteria for 10 days in the fermentor 1000 mL mounted with the biogas collecting balloon. Variables observed in this study were the measurements of biogas volume, culture medium pH, and biogas burning temperature, and the testing of biogas flame colour. Based on the observation result, three of the six examined treatments which could able to produce biogas were P2, P3 and P4 with the respective volume of 160 cm3, 143 cm3, and 118 cm3. While, the measured values of culture medium pH and biogas burning temperature were 6,87-7,10 and 152oC with the flame of red colour. This study shows that the optimal mixture ratio of the basic substrate and the inoculum for producing biogas is 3:2, and the produced biogas is potential to be used as an alternative energy.
PENGAMATAN PERTUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq). P.kummer) PADA MEDIA DASAR SERBUK GERGAJI DENGAN SUPLEMENTASI AMPAS SAGU Wahyu Hidayat; Umrah Umrah; Meryany Ananda
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.415 KB) | DOI: 10.22487/bioceb.v13i3.14966

Abstract

The study of observation of growth media formulation of white oyster mushrooms’s mycelium (Pleurotus ostreatus (Jacq.) P.Kummer) on the base with sago pulp supplementation medium was conducted from March to May 2018 at Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. This study were aimed to determine the appropriate formulation and dosage as supplementation of sago pulp with basic media for the growth of the white oyster mushroom mycelium. This study was conducted by Randomized Complete Random Design (RAL), consisted of seven treatments and four replications with basic media and supplementary M0 (100%: without supplements), M1 (95% : 5%), M2 (90% : 10%), M3 (85% : 15%), M4 (80% : 20%), M5 (75% : 25%), M6 (70% : 30%). The results showed that the best growth of mycelium on treatment M0 and M1, mycelium filled the media for 26 days with the average growth of mycelium M0 and M1 were 0,56 cm/ day. The best formulation and dosage of sago suplementation on the base medium were found in M1 (95% base medium: 5% sago pulp).
FORMULASI LIMBAH SABUT KELAPA DAN KOTORAN TERNAK MENJADI BIOKOMPOS BAHAN AKTIF Aspergillus sp. Fitriani Fitriani; Umrah Umrah; Abdul Rahim Thaha
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.781 KB) | DOI: 10.22487/bioceb.v13i3.14967

Abstract

The Research on formulation of coconut fiber waste and animal manure into biocomposes (decomposer: Aspergillus sp.) has been carried out in Langaleso Village, Dolo Subdistrict, Sigi Regency, Central Sulawesi, Biotechnology Laboratory of the Department of Biology and the Soil Science Laboratory, Faculty of Agriculture at the University of Tadulako Palu, in March to August 2018. The purpose of this research was to formulate the basic substrate (BS) of coconut fiber waste and animal manure supplementation into biocompost using decomposer: Aspergillus sp. The study was designed in a completely randomized design consisting of five treatments and four replications. The treatments arrangement is a comparison of the base substrate of coconut fiber (BS) with animal manure supplements as follows; P0 (BS 100%, without supplements), P1 (BS 90% + supplement 10%), P2 (BS 80% + supplement 20%), P3 (BS 70% + supplement 30%) and P4 (BS 60% + supplements 40%). Parameters of observation include; physical indicators (aroma, color and texture), biology (fungi identification) and chemistry (analysis of C-organic content and nitrogen). The results showed that the P4 treatment showed the best biocompost quality with physical indicators (compost-smelling aroma, black in color and soft texture), biological indicators (identified as Aspergillus sp and Mucor sp contaminant fungi), chemical indicators (40.5% C-organic) , N 1.36% and 29.7%).
PRODUKSI BIOGAS DARI SUBSTRAT DASAR KOTORAN SAPI, KOTORAN AYAM DAN LIMBAH CAIR TAHU DENGAN INOKULUM RUMEN SAPI Subhan nuradzan; Umrah; Kasman
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.225 KB) | DOI: 10.22487/bioceb.v13i3.14968

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The research of biogas aims to obtain an alternative energy source which might replace fossil energy in the future. It was carried out starting from November 2017 to February 2018 in which was located in Langaleso Village, Dolo Subdistrict, Sigi District and in the Laboratory of Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. The primary substrates were cow dung, chicken manure and the tofu liquid waste by cow rumen as inoculum. The installation of gas-flowed pipes was conducted by using paralon pipes with size ½ dan ¾ inch with the total lenght 8,40 m. The incubation period of the primary substrates was 30 days where the biogas volume quantification was periodically performed in the day of 10, 20, and 30. The results showed that biogas was formed at the incubation day of 10 which hence increase up to the day of 30. The observed gas volume was 223,568 cm3, 368,950 cm3 dan 458,302 cm3 respectively. The results of flame test representing blue flame indicated that the formed biogas contained the content of methane which higher than 70%. This hence revealed that the installation of biogas reactor with the type of “fixed domed plant” equipped by “watertrap” was able to produce a good quality biogas from the primary substrates were cow dung, chicken manure and the tofu liquid waste by cow rumen as inoculum for the application in the household scale.
PENGAMATAN GEJALA INFEKSI Phytophthora palmivora PENYEBAB PENYAKIT BUSUK BUAH PADA KAKAO Nurfianti; Umrah
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.411 KB) | DOI: 10.22487/bioceb.v13i3.14969

Abstract

Phytophthora palmivora is a patogen fungi causing pod rot disease in cacao plants. Thr researchof effectivity examination of biopesticide (active ingredient Aspergillus sp.) againts the growth of P. Palmivora in cacao pod (Theobroma cacao L.) by in vivo state was conducted from March 2018 to September 2018. This research was carried out in Sidondo Village, Dolo Subdistrict, Sigi Biromaru District, Central Sulawesi Province and Laboratory of Biotechnology, Faculity of Mathematics and Natural Sciences, Tadulako University. This study was aimed to determine an effective biopesticide (active ingredient Aspergillus sp.) which suppresses the growth of P. Palmivora in cacao pod by in vivo state and to stipulate the most affective biopesticide concentration (active ingredient Aspergillus sp.) in suppressing the growth of of P. Palmivora in cacao pod by in vivo state. This This research was by using the methods of Group Random Design (GRD) or Rancangan Acak Kelompok (RAK) consisting of six treatmentsand four repetitions. The observation parameters consist of symptoms of disease, the lenght of infection spots, the infection persentage of P. Palmivora, microscopic observations, in the microscopic of P. Palmivora. The results showed that the biopesticide (active ingredient Aspergillus sp.) was varified as effective in controlling P. Palmivora drawing the rot disease bin cacao plants, by the absent of any symptoms of infection P1,P2,P3,P4 dan P5 treatments. The only treatments in which was infected by P. Palmivora , drawing the rot disease in cacao plants, was P0 with the occurence time of infection symptoms was in the third day after inoculation, the average lenght of infection spots was 8,65 cm and the average infection persentage was 11,60%.
The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum Nisfaun Safitriana; Umrah; Orryani Lambui
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.187 KB) | DOI: 10.22487/bioceb.v13i3.14971

Abstract

The study about the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.
Formulation of Inoculum a white oyster Mushroom (Pleurotus ostreatus) on powder Khadijah; Umrah; Orryani Lambui
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.956 KB) | DOI: 10.22487/bioceb.v13i3.14972

Abstract

The study about the formulation of inoculum a white oyster mushroom (Pleurotus ostreatus) on flour. was conducted at Biotechnology laboratory of Departement , Faculty of Mathematic and Natural Science, Tadulako University. This study were aimed to oyster mushroom on production. on and to abridge the cycle of oyster mushroom production process of white.peplication used Compeletely Randomized Design (CRD) consist of six treatments and tree by comparing between (sawduts,rice,bran, and comflour) P1(50% : 27% : 23% , P2 (50% : 30% : 20%), P3 (50% : 33% : 17%), P4 (50% : 36% : 14% ), P5 (50% : 39% : 11%), P6 (50% : 42% : 8%). There are four parameters used in this study (1) .The growth of white oyster mushroom inoculum (2) the incubation time. and, (3) coloni forming unit (CFU), (4) Viability test .The resulth showed that the best growth mycelium was 1,3cm/days with incubation period was 26 days, the highest total of CFU in P5 was 12,7x 108 CFU/ml and was in P3 was 3,3 x 108 CFU/ml .The inoculum viability test of production media in P5, was faster in incubation period is 18 days.
FORMULASI MEDIA PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq) P. Kumm) DENGAN SUPLEMENTASI LIMBAH SABUT KELAPA Sitti Masyitah; Umrah Umrah
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15085

Abstract

The research entitled Formulation of media for growth of white oyster mushroom mycelium ((Pleurotus ostreatus (Jacq) P. Kumm) with waste of coconut fiber supplementation was carried out out in Biotechnology Laboratory unit, Department of Biology, Faculty of Mathematics and Natural Sciences. This research purposes; (a) To find out the formulation of coconut fiber waste as a supplementary medium for growing white oyster mushrooms (P.ostreatus); (b) to find out the formulation at the maximum dose of coconut husk supplementation in the medium for good growth of white oyster mushrooms (P.ostreatus). This study was designed based on a Completely Randomized Design (CRD) consisting of 7 settings and 4 replications. The help arrangement is given between Sawdust: Rice Bran: Lime: Coconut Fiber Waste. Namely M0 (Basic media 100%, without Supplements), M1 Basic media 95% + Supplements 5%, M2 (Basic media 90% + Supplements 10%), M3 (Basic media 85% + Supplements 15%), M4 (Basic media 80% + Supplement 20%), M5 (Basic media 75% + Supplement 25%), M6 (Basic media 70% + Supplement 30%). Observation parameters; (a) prohibiting mycelium; (B) Incubation time; (c) Number of colonies (Colony Forming Units); (d) Macroscopic and microscopic observations of mycelium. The results showed that the fastest mycelium growth in M6 treatment was 1.193 cm and the lowest was M0 treatment which was 1.123 cm. The lowest incubation time at M6 is 28 days and the lowest at M0 is 44 days. The highest number of colonies (CFU) in M6 treatment was 1.96 x 10 -12 CFU / g and the lowest was maintenance of M3 1.03 x 10-12 CFU / g.
Co-Authors A. St. Latifah Humairah A AA Sudharmawan, AA Adhiwijaya, Ardian Adi Putra, Andi Budiyanto Aisah Jamili Alwi Alwi Alwi Millang Amalia Purnamasari Zainal Amiruddin Kasim Andi Lindhemuthianingrum Annawaty Annawaty, Annawaty Asri Ismail Asri Pirade Paserang Asriani Hasanuddin Asrul Asrul Asrul Asrul Bidayanti Ruru Budi Indarsih, Budi Burhan, Kurnia Dana Sulistia Dewi, Ni Made Aryani Saraswati Dhini Arwindah Dian Sri Anugrah Djoko Kisworo Eka Fitriani Eny Yuniati Evisilvia Evisilvia Fahri Fahri Febiyanti Firdasari Firdasari Firji Della Sugeha Fitri Kurnianingsih Fitriani Fitriani Fitriani Husain Gina, Fina Alimatul Hardianti Lestari Hamid Hasriani Hasriani Humaero, Siti I Nengah Suwastika I Nyoman P. Aryantha Ika Merdeka Wati Ikha Wahyuni Ilham Bakri Abdul Rahim Thaha,Isrun, Ilham Bakri Irawan, Adit IRWAN LAKANI Jamaluddin Jamaluddin Jaya, I Nyoman Sukartha Jesica Pasaribu Juliana Juliana Kasman Sulaiman Khadijah Kirana Kirana Laksono Trisnantoro Lamadjido, Sri Rahayu Latifah Merdekawaty Lina Mahardiana Makmur, Baitul Mardiana Upara Maulana, Ardian Maulida, Nurhidayatul Meryany Ananda Miranty Sirappa Miswan Miswan Moh. Iqbal, Moh. Mufida Al Idrus Muh. Arsal N. Maddo Muhaemin Muhaemin Muhammad Alwi Muhammad Amiruddin Muhammad Saleh Muhammad Yusril Basir Murnawati Murnawati Musdalifa Musdalifa Musjaya M. Guli Mustafid Rasyiid Mutma Inna Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Mutmainah Naksabandi, Roydatul Robbi Ni Ketut Dewi Haryani, Ni Ketut Dewi Niluh Budi Artini Nisfaun Safitriana Nova Damayanti Novika Nur Asrinawaty Nur Hikma Nurfianti Nurhayati N Mangopo Nurvita Nurvita Nurzakia Nurzakia Orryani Lambui Rahayu Rahayu Rahmania Rahmania Rahmiati Rahmiati Ramadanil Pitopang Rifka Rifka Rizki Purnamasari Rizkita Rachmi Esyanti Roliana Roliana Saparudin Saparudin Septian, I Gede Nano Setia Ayu Lestari Sitti Ardianti Sitti Masyitah Sri Utami Subhan nuradzan Tamzil, Moh Hasil Tapaul Rozi Tjandra Anggraeni Tjandra Anggraini Tri Marsha Hasibuan Triana, Nina Risnanda Trismansyah Trismansyah Wahyu Harso Wahyu Hidayat Wasilahturahman Widiawati Yulia Astuti Yusran Yusran Yustilawati, Eva