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<![CDATA[PERUBAHAN KUALITAS SPERMATOZOA DAN JUMLAH SEL-SEL SPERMATOGENIK TIKUS YANG TERPAPAR ASAP ROKOK ]]>
<![CDATA[Adrien Jems Akiles Unitly]]>;
<![CDATA[Nastiti Kusumorini]]>;
<![CDATA[Srihadi Agungpriyono]]>;
<![CDATA[Aryani Sismin Satyaningtijas]]>;
<![CDATA[Arief Boediono]]>
<![CDATA[ Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September ]]>
Publisher : <![CDATA[Universitas Syiah Kuala ]]>
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DOI: 10.21157/j.ked.hewan.v8i2.2629
<![CDATA[Penelitian ini bertujuan mengetahui efek asap rokok terhadap perubahan kualitas spermatozoa dan jumlah sel-sel spermatogenik. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan perlakuan asap rokok 10 batang/ekor/hari selama 2,5 jam dalam smoking chamber terhadap 24 ekor tikus jantan yang dibagi menjadi 4 kelompok, masing-masing 6 ekor dalam tiap kelompok. Kelompok P1 adalah kelompok yang tidak diberi perlakuan (kontrol), P2 adalah kelompok yang dipapar asap rokok selama 20 hari, P3 adalah kelompok yang dipapar asap rokok selama 40 hari, P4 adalah kelompok yang dipapar asap rokok selama 60 hari. Parameter yang diamati adalah kualitas spermatozoa dan jumlah sel-sel spermatogenik. Hasil penelitian menunjukkan bahwa pemaparan asap rokok dapat menurunkan konsentrasi spermatozoa dan viabilitas spermatozoa serta meningkatkan abnormalitas spermatozoa. Disimpulkan bahwa pemaparan asap rokok 10 batang/ekor/hari menyebabkan penurunan kualitas spermatozoa dan jumlah sel-sel spermatogenik yang tidak kembali ke kondisi normal setelah proses penyembuhan.]]>
<![CDATA[Efek Antidiabetes Yoghurt Pala Terhadap Penurunan Kadar Glukosa Darah Tikus Model Diabetes Melitus Tipe 2 ]]>
<![CDATA[Unitly, Adrien Jems Akiles]]>;
<![CDATA[Baszary, Chomsa Dintasari Umi]]>;
<![CDATA[Moniharapon, Debby D.]]>;
<![CDATA[Watuguly, Theopilus W.]]>;
<![CDATA[Moniharapon, Mechiavel]]>;
<![CDATA[Eddy, La]]>;
<![CDATA[Lesbatta, Kezia Josawel]]>;
<![CDATA[Sillehu, Eka Safitri]]>;
<![CDATA[Sikafir, Beatrix Belina]]>;
<![CDATA[Sopacua, Gracia N.]]>
<![CDATA[ Kalwedo Sains (KASA) Vol 5 No 2 (2024): Kalwedo Sains (KASA), September 2024 ]]>
Publisher : <![CDATA[Program Studi Di Luar Kampus Utama Universitas Pattimura ]]>
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DOI: 10.30598/kasav5i2p91-98
<![CDATA[Diabetes Melitus tipe 2 adalah penyakit gangguan metabolik yang di tandai oleh kenaikan gula darah akibat penurunan sekresi insulin oleh sel beta pankreas atau gangguan fungsi insulin (resistensi insulin). Potensi yoghurt dalam menurunkan kadar glukosa darah berhubungan dengan kemampuan bakteri asam laktat dalam yoghurt menurunkan kadar kolesterol dan plasma lipid, sehingga meningkatkan sensitivitas insulin pankreas. Buah pala mengandung senyawa kimia yang dapat menunjukkan sifat antioksidan dan antibakteri. Tujuan penelitian ini untuk mengetahui efek terapi yoghurt pala terhadap penurunan kadar glukosa darah tikus model diabetes melitus tipe 2. Penelitian ini menggunakan rancangan acak lengkap (RAL). Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari lima perlakuan dan tiga kali ulangan, yaitu K(-): Kelompok kontrol negatif yaitu kelompok tikus yang diberi STZ, K(+): Kelompok kontrol positif yaitu kelompok tikus yang diberi STZ 45 mg/ekor/hari kemudian diberikan obat glibenklamid selama 14 hari, 1.25: Kelompok tikus yang diberi STZ 45 mg /ekor/hari kemudian diberi ekstrak yoghurt pala 1.25 ml /ekor/hari selama 14 hari, 2.5: Kelompok tikus yang diberi STZ 45 mg /ekor/hari kemudian diberi ekstrak yoghurt pala 2.5 ml/ekor/hari selama 14 hari dan 5.0: Kelompok tikus yang diberi STZ 45 mg /ekor/hari kemudian diberi ekstrak yoghurt pala 5 ml/ekor/hari selama 14 hari. Hasil penelitian menunjukkan pemberian yoghurt pala terbukti memiliki efek antioksidan dalam menurunkan kadar glukosa darah dimana dosis yoghurt pala yang efektif adalah 5.0 ml.]]>
<![CDATA[THE EFFECT OF ADMINISTRATION ETHANOL EXTRACT KEBAR'S GRASS (Biophytum petersianum K.) ON NUMBER OF EMBRYOS AND CHILD FROM RATS PARENT THAT EXPOSED TO CIGARETTE SMOKE ]]>
<![CDATA[Laratmase, Maria]]>;
<![CDATA[Unitly, Adrien Jems Akiles]]>;
<![CDATA[Suriani, Sanita]]>
<![CDATA[ Biofaal Journal Vol 1 No 1 (2020): Biofaal Journal ]]>
Publisher : <![CDATA[Pattimura University ]]>
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DOI: 10.30598/biofaal.v1i1pp1-8
<![CDATA[Rumput kebar merupakan tanaman yang mengandung senyawa aktif seperti flavonoid, vitamin A, dan vitamin E yang diduga mampu mengatasi dampak buruk radikal bebas berupa asap rokok. Tujuan dari penelitian ini adalah untuk mengetahui efek pemberian ekstrak etanol rumput kebar (Biophytum petersianum K.) terhadap jumlah embrio dan anak tikus Rattus norvegicusyang terpapar asap rokok. Penelitian mengggunakan metode eksperimental laboratorik dengan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan yang masing-masing diulang tiga kali, yaitu (-): Kelompok kontrol negatif yaitu tikus yang tidak diberi perlakuan, (+): Kelompok kontrol positif yaitu tikus dipaparkan asap rokok selama 28 hari, (0.067): Kelompok tikus yang dipapar asap rokok selama 28 hari kemudian diberi ekstrak etanol rumput kebar dosis 0.067mg/ekor/hari selama 28 hari, dan (0.135): Kelompok tikus yang dipapar asap rokok selama 28 hari kemudian diberi ekstra ketanol rumput kebar dosis 0.135mg/ekor/hari selama 28 hari. Setelah perlakuan asap rokok dan pemberian ekstrak etanil rumput kebar, tikus dikawinkan. Pengamatan jumlah embrio dilakukan pada saat masa kebuntingan ke 12 hari kemudian dibedah, sedangkan pengamatan jumlah anak dilakukan pada saat anak tikus dilahirkan. Data yang diperoleh dianalisis dengan Analysis of Variance (ANOVA)-SPSS versi 16.0 dilanjutkan dengan uji Duncan dengan selang kepercayaan 95% (α = 0.05). Hasil penelitian menunjukan bahwa pemberian ekstrak etanol rumput kebar dengan dosis 0.135mg/ekor/hari mampu menjaga keberhasilan implantasi embrio ke dinding uterus sehingga rata-rata jumlah embrio dan anak tikus meningkat.]]>
<![CDATA[ANALYSIS OF MILK PRODUCTION RAT Rattus norvegicus EXPOSED TO CIGARETTE SMOKE AFTER ADMINISTRATION OF ETHANOL EXTRACT OF KEBAR'S GRASS (Biophytum petersianum Klotzsch) ]]>
<![CDATA[Idris, Kurniawati]]>;
<![CDATA[Unitly, Adrien Jems Akiles]]>
<![CDATA[ Biofaal Journal Vol 1 No 1 (2020): Biofaal Journal ]]>
Publisher : <![CDATA[Pattimura University ]]>
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DOI: 10.30598/biofaal.v1i1pp19-26
<![CDATA[Rumput kebar merupakan tumbuhan yang mengandung asam folat, vitamin A, vitamin C dan vitamin E serta golongan senyawa aktif seperti flavonoid yang diduga mampu mengatasi dampak buruk radikal bebas berupa asap rokok. Tujuan dari penelitian ini adalah untuk mengetahui efek pemberian ekstrak etanol rumput kebar rumput kebar (Biophytum petersianum Klotzsch) terhadap analisis produksi air susu pada tikus Rattus norvegicus yang terpapar asap rokok. Penelitian menggunakan metode eksperimental yaitu pola rancangan acak lengkap (RAL) dengan 4 (empat) kelompok perlakuan dan 3 (tiga) kali ulangan. Tikus kelompok kontrol negatif diberi makan secara normal dan dikawinkan pada awal penelitian, tikus kelompok kontrol positif diberi paparan asap rokok selama 28 hari kemudian dikawinkan, sedangkan tikus kelompok dosis esktrak etanol rumput kebar, diberi paparan asap rokok selama 28 hari, dan diberi ekstrak etanol rumput kebar selama 28 har kemudian dikawinkan. Penimbangan bobot badan untuk mengetahui produksi air susu dilakukan selama 14 hari dimulai saat anak berusia 5 hari hingga 19 hari. Pertumbuhan bobot badan anak diukur selama anak menyusu, dan bobot lepas sapih diukur di akhir masa laktasi. Data yang diperoleh dianalisis dengan Analysis of Variance (ANOVA)-SPSS versi 16.0 dilanjutkan dengan uji Duncan dengan selang kepercayaan 95 % (α = 0.05). Hasil penelitian menunjukan bahwa pemberian ekstrak etanol rumput kebar dengan dosis 0.135mg/ekor/hari mampu meningkatkan produksi air susu pada induk tikus betina yang terpapar asap rokok.]]>
<![CDATA[UTILIZATION OF MALE BREADFRUIT (Artocarpus altilis) AS A BIOLARVACIDE FOR Anopheles sp. ]]>
<![CDATA[Moniharapon, Debby Dijola]]>;
<![CDATA[Nindatu, Maria]]>;
<![CDATA[Unitly, Adrien Jems Akiles]]>;
<![CDATA[Sikafir, Beatrix Belina]]>
<![CDATA[ Biofaal Journal Vol 4 No 1 (2023): Biofaal Journal ]]>
Publisher : <![CDATA[Pattimura University ]]>
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DOI: 10.30598/biofaal.v4i1pp31-39
<![CDATA[Penelitian ini bertujuan untuk mengetahui efek biolarvasida ekstrak etanol bunga sukun jantan (Artocarpus altilis) terhadap mortalitas larva nyamuk Anopheles sp. vektor malaria. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 6 perlakuan dan 3 kali ulangan: A : Kontrol negatif (Aquades), B: Kontrol positif (Abate), C: Konsentrasi (Ekstrak etanol bunga sukun jantan) 1%, D: Konsentrasi (Ekstrak etanol bunga sukun jantan) 3%, E: Kosentrasi (Ekstrak etanol bunga sukun jantan) 6%, F: Konsentrasi (Ekstrak etanol bunga sukun jantan) 9%. Hasil yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) kemudian dilanjutkan Uji Duncan pada taraf nyata α=0,05 menggunakan perangkat lunak SAS. Populasi dalam penelitian ini adalah larva Anopheles sp. yang didapat dari tempat perindukan larva nyamuk di dalam rumah (in door). Sampel dalam penelitian ini adalah 450 larva Anopheles sp. instar III pada masing-masing diletakan dalam 18 gelas plastik, yang masing-masing plastik berisi 25 ekor larva Anopheles sp. instar III. Sampel diadaptasikan selama seminggu, kemudian diberi perlakuan selama 12 jam. Hasil penelitian menunjukkan adanya efek biolarvasida Ekstrak etanol bunga sukun jantan (Artocarpus altilis) terhadap larva nyamuk Anopheles sp., konsentrasi ekstrak etanol bunga sukun jantan (Artocarpus altilis) yang efektif adalah 6%, yang mampu membunuh larva nyamuk Anopheles sp. dengan presentasi mortalitias 72% pada jam ke-12, dan 50% mortalitas (LC50) larva Anopheles sp. terjadi pada konsentrasi ekstrak etanol bunga sukun jantan konsentrasi 1.25%.]]>
<![CDATA[Uterine Performance of Rats Exposed to Cigarette Post-Therapy Ethanol Extract of Kebar Grass (Biophytum petersianum Klotzsch) ]]>
<![CDATA[Unitly, Adrien Jems Akiles]]>;
<![CDATA[Kaihena, Martha]]>;
<![CDATA[Kubela, Dormatina]]>
<![CDATA[ Devotion : Journal of Research and Community Service Vol. 4 No. 6 (2023): Devotion: Journal of Research and Community Service ]]>
Publisher : <![CDATA[Green Publisher Indonesia ]]>
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DOI: 10.59188/devotion.v4i6.492
<![CDATA[This study aims to determine the effect of giving ethanol extract of kebar grass (Biophytum petersianum Klotzsch) on the uterine performance of Rattus norvegicus rats exposed to cigarette smoke. The experimental design used was a completely randomized design (CRD). The results obtained were analyzed by Analysis of Variance (ANOVA) then continued with Duncan's test at a significant level of α = 0.05 using SAS software. The sample consisted of 12 rats, which were divided into 4 groups. The rat group (+), 0.067, 0.0135, decreased uterine function by exposure to 10 cigarettes/head/day for 28 days. Mice (-) were the negative control group, namely rats that were not exposed to cigarette smoke and ethanol extract of coriander grass. Mice (+) are the positive control group, namely rats exposed to cigarette smoke 10 cigarettes/head/day for 28 days. Groups 0.067 and 0.135 were exposed to cigarette smoke 10 cigarettes/head/day for 28 days and then given 0.067 mg of coriander grass ethanol extract for the 0.067 group and a dose of 0.135 mg for the rat group 0.135. The results showed that there was an effect of increasing the performance of the rat uterus after administration of ethanol extract of coriander grass. It can be concluded that administration of the ethanol extract of the grass Biophytum petersianum Klotzsch kebar can increase uterine weight and uterine length of rats, with a good dose of 0.135 mg.]]>
<![CDATA[Electric Mosquito Repellent Effect of Male Breadfruit Flower Mat Causes Mosquito Mortality Anopheles sp. ]]>
<![CDATA[Moniharapon, Debby Dijola]]>;
<![CDATA[Kaihena, Martha]]>;
<![CDATA[Unitly, Adrien Jems Akiles]]>
<![CDATA[ Devotion : Journal of Research and Community Service Vol. 4 No. 6 (2023): Devotion: Journal of Research and Community Service ]]>
Publisher : <![CDATA[Green Publisher Indonesia ]]>
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DOI: 10.59188/devotion.v4i6.494
<![CDATA[Through mosquito bites, parasites of the genus Plasmodium spread the disease known as malaria. Malaria-carrying mosquitoes of the genus Anopheles sp. One secure method of controlling the Anopheles sp. mosquito population is biological mosquito control. Since the biological method does not use chemicals but rather the phytochemicals found in plants, one of which contains compounds from the male breadfruit flower and is capable of acting as a vegetable larvicide or mosquito repellent. The aims of the study is to evaluate the effectiveness of electric mosquito mats that contain male breadfruit flowers to kill or cause mosquitoes (Anopheles sp.) to die. A completely randomized design (CRD) was used for the experiment. The results showed a change in the mortality of Anopheles sp. mosquito larvae. After exposure to breadfruit flower mat doses of 0.5gr, 1gr, 1.5gr, 2gr compared to no administration. Thus, it was concluded that male breadfruit flower mats with concentrations of 0.5gr, 1gr, 1.5gr, and 2gr had an insecticidal effect on the mortality of Anopheles sp. mosquitoes. with an effective concentration of 2gr (96.5% mortality), of which 50% mortality (LC50) of Anopheles sp. larvae. Occurred at a concentration of ethanol extract of male breadfruit flowers dose 0.36gr.]]>
<![CDATA[ESTRUS CYCLE OF Rattus norvegicus EXPOSED TO CIGARETTE SMOKE AFTER TREATMENT ETHANOL EXTRACT OF GRASS (Biophytum petersianum Klotzsch) ]]>
<![CDATA[Simatauw, Anniestasya Z]]>;
<![CDATA[Unitly, Adrien Jems Akiles]]>
<![CDATA[ RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/rumphiusv1i1p001-007
<![CDATA[Kebar grass is a plant that contains phytoestrogens which have the same effect as endogenous estrogens which are thought to be able to increase endogenous estrogen levels in the blood due to the adverse effects of free radicals in the form of cigarette smoke. The purpose of this study was to determine the effect of the ethanol extract of kebar grass (Biophytum petersianum Klotzsch) on the length of the estrous cycle of Rattus norvegicus rats exposed to cigarette smoke. The research used an experimental method, namely a completely randomized design with four treatment groups and three replications. Rats the negative group was fed normally, the positive control group was exposed to cigarette smoke for 28 days, while the rats in the Kebar grass ethanol extract dosed group were given cigarette smoke exposure for 28 days, and were gived Kebar grass ethanol extract for 28 days. Vaginal smears were taken in the morning with a span of 24 hours for 28 days. Changes in the vaginal epithelium are examined to determine the phase of the estrous cycle using a microscope. The data obtained were analyzed by Analysis of Variance (ANOVA)-SPSS version 16.0 followed by Duncan test with a 95% confidence interval (α=0.05). The results showed that administration of Kebar grass ethanol extract was able to extend the estrous cycle time at a dose of 0.135 mg/head/day due to prolongation of the proestrus and estrus phases.]]>
<![CDATA[THE STUDY OF ADMINISTRATION EXTRACT KEBAR GRASS ON NUMBER LEYDIG CELLS AND SERTOLI CELLS IN RAT (Rattus norvegicus) THAT EXPOSED WITH CIGARETTE SMOKE ]]>
<![CDATA[Unitly, Adrien Jems Akiles]]>
<![CDATA[ RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/rumphiusv1i1p008-011
<![CDATA[This research aimed to study on the microscopic changes of number leydig cells and sertoli cells. A factorial CRD with periode of treatment and sample collection was applied in this study. An exposure of cigarette smoke was carried out at 10 cigarettes/rat/day for 2.5 hours in a smoking chamber. Extract kebar’s grass given was 0.0945 mg/g body weight/day. Twenty seven (27) of male rats were divided into four groups. N group was untreated animals, 20h group is group that was expose to cigarette smoke for 20 days then given administration extract kebar’s grass for 20 days and stopping treatment for 20 days and 60h group is group that was expose to cigarette smoke for 60 days then given administration extract kebar’s grass 60 days and stopping treatment for 60 days. Data collection was carried out at twice that after cigarette smoke exposure (T0), second after administration extract kebar’s grass (T1) and third after stopping treatment (T2). The parameters measured were the number of leydig cells and sertoli cells. That increasing number leydig cells and sertoli cells of rat exposed to cigarette smoke after gived seaweed extract kebar’s grass for 20 days and]]>
<![CDATA[ACTIVITY OF CLOVE LEAVES (Syzygium aromaticum L.) LARVACIDES ON MORTALITY OF Anopheles sp. ]]>
<![CDATA[Sapulette, Fenska Violenta]]>;
<![CDATA[Unitly, Adrien Jems Akiles]]>;
<![CDATA[Moniharapon, Debby]]>
<![CDATA[ RUMPHIUS Vol 1 No 2 (2019): RUMPHIUS Pattimura Biological Journal ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/rumphiusv1i2p042-046
<![CDATA[This study used a completely randomized design with a sample of 525 Anopheles sp. larvae. which were divided into 7 groups and 3 replicates, which contained 25 Anopheles spper larvae. Except for the control, each group was given clove leaf infusion starting from a concentration of 0.50%, 1%, 2%, 4% and 6%, where the treatment was carried out for 24 hours. The results showed that there was a larvicidal effect of brewing clove leaves (Syzygium aromaticum L.) on the mortality of Anopheles sp mosquito larvae, where the effective concentration was 6% and had an LC50 value in killing Anopheles sp larvae of 0.89%. The overall phytochemical content of clove leaves which has a fast response to the mortality of Anopheles sp. are saponins, flavonoids and tannins which are capable of acting as larvicides through the mechanism of damaging cell membranes or interfering with larval metabolic processes. The content of saponins, flavonoids and tannins can increase the mortality of Aedes aegypti L. larvae through the mechanism of damaging cell membranes or interfering with larval metabolic processes as stomach poisoning.]]>
