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Pengelolaan Ekosistem Lamun dengan Metode Teknologi Terf dan Sprig Anchor Untuk Keberlanjutan Desa Wisata Pahawang, Kabupaten Pesawaran Nur Muhammad Tirta Weuning Al Supandi; Hilma Nahwa Firdausi; Maria Febriyanti Samosir; Kristian Thomas Tefsele; Dwi Okta Viani; Sheliyana Cahya Oktari; Farhan Juliputra Kaisinda; Deliza Putri; Widya Asri; Sabrina Cinta Farani; Rizqy Hadi Saputra; Alifia Adibila Nurhalisa; Indra Jaya; Deny Sapto Chondro Utomo; Maulid Wahid Yusup
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 2, No 2 (2023): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 2 No 2, September 2
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v2i2.7989

Pesawaran merupakan salah satu kabupaten dengan sebaran potensi objek wisata yang beragam di sejumlah daerah, salah satunya yaitu Pulau Pahawang. Penurunan luasan padang lamun yang terjadi setiap tahunnya disebabkan oleh berbagai aktivitas manusia. Aktivitas tersebut diantaranya yaitu pembangunan konstruksi pesisir, pembuangan limbah domsetik, alur perjalanan kapal, dan juga penangkapan ikan. Pelaksanaan kegiatan pengabdian kepada masyarakat dilaksanakan di Desa Wisata Pahawang, Kabupaten Pesawaran. Kegiatan ini melibatkan masyarakat sekitar Desa Wisata Pahawang dan Himpunan Mahasiswa Perikanan dan Kelautan Fakultas Pertanian Universitas Lampung. Kontribusi masyarakat sebagai peserta dalam kegiatan transplantasi lamun serta melakukan kegiatan monitoring persentase pertumbuhan lamun. Metode transplantasi yang dapat dilakukan yaitu metode terf dan sprig anchor. Jumlah bibit yang ditanam menggunakan metode terf sebanyak 350 bibit dengan 14 plot frame dimana masing-masing plot berisi 25 bibit. Pada metode sprig anchor, jumlah bibit yang ditanam sebanyak 475 bibit dengan 19 plot dimana masing-masing plot berisi 25 bibit. Jenis lamun yang ditanam yaitu Enhalus acoroides dengan ciri morfologi tumbuhan yaitu daun panjang menyerupai pita dengan ujung daun membulat. Pemulihan kondisi ekosisitem lamun dilakukan dengan kegiatan transplantasi lamun menggunakan dua metode yaitu terf dan sprig anchor. Kegiatan pengabdian masyarakat pelestarian ekosistem lamun diterima baik oleh masyarakat Desa pahawang dan dapat dilanjutkan oleh masyarakat untuk menjaga kelestarian lingkungan serta keberlangsungan makhluk hidup yang tinggal di wilayah pesisir.

Mitigasi Penyakit Mikrosporidia Enterocytozoon Hepatopenaei (EHP) Pada Tambak Masyarakat Di Desa Purworejo, Pasir Sakti, Lampung Timur Fidyandini, Hilma Putri; Elisdiana, Yeni; Sarida, Munti; Utomo, Deny Sapto Chondro; Viani, Dwi Okta
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 3, No 2 (2024): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 3 No 2, September 2
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v3i2.9753

Kegiatan pengabdian kepada masyarakat berjudul "Mitigasi Penyakit Mikrosporidia Enterocytozoon Hepatopenaei (EHP) pada Tambak Masyarakat di Desa Purworejo, Pasir Sakti, Lampung Timur" bertujuan untuk meningkatkan pengetahuan dan keterampilan petambak dalam menangani penyakit EHP yang mengancam produktivitas tambak. Kegiatan ini dilakukan melalui serangkaian kegiatan yang meliputi materi tentang identifikasi gejala, teknik pencegahan, dan pengelolaan tambak yang baik. Evaluasi dilakukan dengan pretest dan posttest yang melibatkan 30 peserta. Hasil menunjukkan peningkatan signifikan, di mana nilai pretest yaitu 23,33, kemudian hasil postest sebesar 71,67 dimana peserta memiliki pemahaman yang baik setelah pelatihan. Umpan balik dari peserta menunjukkan antusiasme dan keinginan untuk menerapkan pengetahuan baru dalam praktik. Kegiatan ini diharapkan dapat memberikan dampak positif bagi pengelolaan tambak di desa, serta meningkatkan ketahanan ekonomi masyarakat.

Comparative Test of Ovaprim, Spawnprim, and HCG in the Process Spawning of Pangasianodon hypophthalmus Leonita, Vinka; Utomo, Deny Sapto Chondro; Fidyandini, Hilma Putri
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.17-25

Pangasianodon hypophthalmus is a type of freshwater fish that has potential and has economic value. Its production target which increases every year causes farmers to spawn with the help of hormonal stimulation. The aimed of this research was to test and find out the results of the use of three different hormones namely Ovaprim, Spawnprim, and HCG in P. hypopthalmus spawning. This research was conducted in January 2020 at the Trimurjo Fish Seed Center, Central Lampung. This research used a randomized complete design method with the 3 treatments, P1 (Ovaprim 0,5 ml / kg), P2 (Spawnprim 0,5 ml / kg), P3 (HCG 500 IU / kg) and 3 replications. The results of this research indicate a significant difference (p <0,05) in the relative fecundity parameters, fertilization rate, and hatching rate, but not significantly different in the parameters of latensi periode and diameter of eggs. In P1, Ovaprim hormone injections provide better reproductive performance compared to Spawnprim and HCG injections.

The Technique of Directing the Sex of Betta Fish (Betta Sp.) Larval Stage (H3, H5, and H7) using Coconut Water (Cocos nucifera) Rohayati, Cucu; Utomo, Deny Sapto Chondro; Adiputra, Yudha Trinoegraha
Jurnal Perikanan dan Kelautan Vol. 26 No. 3 (2021): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.3.185-191

Betta (Betta sp.) is an anabantidae fish with sexual development that can be directed at the embryonic and larval stages. One way to direct the sex development of these fish is by using hormone therapy using natural ingredients. Coconut water is one of the natural ingredients that can be used because it contains high potassium. This potassium can convert cholesterol in fish body tissue to pregnenolone which will affect the increase in testosterone production. This study aims to determine the best dose and age group in directing the sex of Betta fish experimentally using a randomized block design (RBD) with 4 treatments and 3 replications. The immersion treatment of larvae in the age group of 3 days, 5 days, and 7 days for 15 hours with a dose of 0%, 10%, 20%, and 30% coconut water was carried out in a 3 L jar containing 30 larvae. The results showed that coconut water was able to influence the increase in the percentage of male betta fish (P <0.05) with the highest value at a dose of 30% as much as 86.26 ± 8.34% and the 3 day larvae age group of 72.78 ± 16.03, and have a different effect on other treatments. In addition, the survival calculated after immersion shows a value of 100%, meaning that coconut water does not have a harmful effect on fish. Based on the results of this study, the best treatment for directing the sex of betta fish is a dose of 30% coconut water with the 3 day age group.

Production and bioactivity test of recombinant protein common carp growth hormone Utomo, Deny Sapto Chondro; Alimuddin, .; Sudrajat, Agus Oman; Faizal, Irvan
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study aimed to produce recombinant growth hormone (rGH) protein of common carp (Cyprinus carpio) and evaluate its bioactivity. DNA fragment encoding mature GH protein of common carp (mCcGH) was amplified by polymerase chain reaction (PCR) method and PCR products were then ligated into pCold I to generate pCold I-mCcGH protein expression vector. Escherichia coli BL21 (DE3) harboring pCold I-mCcGH was cultured in the 2xYT medium at 15 °C for 24 hours and protein production was induced by isopropyl-beta-thio galactopyranoside (IPTG). The inclusion bodies containing rGH protein from E. coli transformants were isolated by sonication method and analyzed by SDS-PAGE. The result showed that rGH with molecular weight of about 25 kDa was obtained. Common carp juveniles with average body weight of 5.2±0.4 g were intramuscularly injected once a week for 4 weeks with rGH protein solution from 1 μg bacterial cells per gram fish body weight. The result showed that juveniles fish injected with rGH grew 106.56% higher than control. This result indicated that rGH produced in E. coli BL21 possessed biological activity and it may be useful to improve growth of aquaculture species.Key words: growth hormone, recombinant protein, common carp ABSTRAKPenelitian ini bertujuan menghasilkan protein rekombinan hormon pertumbuhan (growth hormone, GH) dari ikan mas (Cyprinus carpio) dan menguji bioaktivitasnya. Fragmen DNA penyandi protein matang (mature) GH ikan mas (mCcGH) diamplifikasi dengan menggunakan metode PCR dan hasilnya kemudian diligasi ke dalam pCold-I untuk menghasilkan konstruksi vektor ekspresi pCold-I-mCcGH. Plasmid pCold-I-mCcGH ditransformasi ke bakteri Escherichia coli BL21 (DE3), dikultur dalam media 2xYT cair pada suhu 15°C selama 24 jam dan produksi protein diinduksi dengan menggunakan isopropyl-beta-thio galactopyranoside (IPTG). Badan inklusi yang mengandung protein rekombinan GH (rGH) dari bakteri E. coli transforman diisolasi menggunakan metode sonikasi dan dianalisis dengan menggunakan SDS-PAGE. Hasil penelitian menunjukkan bahwa rGH dengan bobot molekul sekitar 25 kDa berhasil diproduksi. Benih ikan mas dengan bobot rata-rata 5,15±0,4 g diinjeksi secara intramuskular satu kali per minggu selama 4 minggu dengan larutan rGH hasil ekstraksi dari 1 µg pelet bakteri/g bobot ikan. Benih yang disuntik dengan rGH tumbuh sekitar 100% lebih cepat bila dibandingkan dengan kontrol yang tidak diinjeksi rGH. Hasil ini mengindikasikan bahwa rGH yang diproduksi dalam bakteri E. coli memiliki bioaktivitas dan dapat bermanfaat untuk memacu pertumbuhan spesies ikan-ikan budidaya.Kata kunci: hormon pertumbuhan, protein rekombinan, ikan mas

Comparative Test of Ovaprim, Spawnprim, and HCG in the Process Spawning of Pangasianodon hypophthalmus Leonita, Vinka; Utomo, Deny Sapto Chondro; Fidyandini, Hilma Putri
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Pangasianodon hypophthalmus is a type of freshwater fish that has potential and has economic value. Its production target which increases every year causes farmers to spawn with the help of hormonal stimulation. The aimed of this research was to test and find out the results of the use of three different hormones namely Ovaprim, Spawnprim, and HCG in P. hypopthalmus spawning. This research was conducted in January 2020 at the Trimurjo Fish Seed Center, Central Lampung. This research used a randomized complete design method with the 3 treatments, P1 (Ovaprim 0,5 ml / kg), P2 (Spawnprim 0,5 ml / kg), P3 (HCG 500 IU / kg) and 3 replications. The results of this research indicate a significant difference (p <0,05) in the relative fecundity parameters, fertilization rate, and hatching rate, but not significantly different in the parameters of latensi periode and diameter of eggs. In P1, Ovaprim hormone injections provide better reproductive performance compared to Spawnprim and HCG injections.

Comparative Test of Ovaprim, Spawnprim, and HCG in the Process Spawning of Pangasianodon hypophthalmus Leonita, Vinka; Utomo, Deny Sapto Chondro; Fidyandini, Hilma Putri
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Pangasianodon hypophthalmus is a type of freshwater fish that has potential and has economic value. Its production target which increases every year causes farmers to spawn with the help of hormonal stimulation. The aimed of this research was to test and find out the results of the use of three different hormones namely Ovaprim, Spawnprim, and HCG in P. hypopthalmus spawning. This research was conducted in January 2020 at the Trimurjo Fish Seed Center, Central Lampung. This research used a randomized complete design method with the 3 treatments, P1 (Ovaprim 0,5 ml / kg), P2 (Spawnprim 0,5 ml / kg), P3 (HCG 500 IU / kg) and 3 replications. The results of this research indicate a significant difference (p <0,05) in the relative fecundity parameters, fertilization rate, and hatching rate, but not significantly different in the parameters of latensi periode and diameter of eggs. In P1, Ovaprim hormone injections provide better reproductive performance compared to Spawnprim and HCG injections.

The Technique of Directing the Sex of Betta Fish (Betta Sp.) Larval Stage (H3, H5, and H7) using Coconut Water (Cocos Nucifera) Rohayati, Cucu; Utomo, Deny Sapto Chondro; Adiputra, Yudha Trinoegraha
Jurnal Perikanan dan Kelautan Vol. 26 No. 3 (2021): October
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Betta (Betta sp.) is an anabantidae fish with sexual development that can be directed at the embryonic and larval stages. One way to direct the sex development of these fish is by using hormone therapy using natural ingredients. Coconut water is one of the natural ingredients that can be used because it contains high potassium. This potassium can convert cholesterol in fish body tissue to pregnenolone which will affect the increase in testosterone production. This study aims to determine the best dose and age group in directing the sex of Betta fish experimentally using a randomized block design (RBD) with 4 treatments and 3 replications. The immersion treatment of larvae in the age group of 3 days, 5 days, and 7 days for 15 hours with a dose of 0%, 10%, 20%, and 30% coconut water was carried out in a 3 L jar containing 30 larvae. The results showed that coconut water was able to influence the increase in the percentage of male betta fish (P <0.05) with the highest value at a dose of 30% as much as 86.26 ± 8.34% and the 3 day larvae age group of 72.78 ± 16.03, and have a different effect on other treatments. In addition, the survival calculated after immersion shows a value of 100%, meaning that coconut water does not have a harmful effect on fish. Based on the results of this study, the best treatment for directing the sex of betta fish is a dose of 30% coconut water with the 3 day age group.

Biological Population of Torpedo Scad (Megalaspis cordyla) in The Waters of Lampung Bay Kartini, Nidya; Caesario, Rachmad; Utomo, Deny Sapto Chondro; Delis, Putu Cinthia
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8943

Megalaspis cordyla, a demersal fish popularly referred to as torpedo scad, is the most commonly caught fish. As market demand and consumption rise, this will have an adverse effect on the sustainability of fish resources if improperly managed. The aim of this research was to analyze the population dynamics of Megalaspis cordyla in the waters of Lampung Bay. The study was carried out between April and September of 2024. Fishermen's catches in the waters surrounding Lampung Bay provided the Bekre fish used in the study. The study's findings demonstrated that bekre fish have a negative allometric growth trend. The value of the condition factor falls between 0.631 and 0.966. Fishing mortality (F) is 3.46 per year, total mortality (Z) is 4.59 per year, and exploitation rate (E) is 0.75 per year. The natural mortality value (M) of bekre fish is 1.13 per year, as are the values of the growth parameters of bekre fish, specifically Linf of 456 mm, growth coefficient (K) of 1.51 per year, and t0 of -0.049. March and October saw the highest bekre fish recruitment rates in Lampung Bay's waters, with 32.55% and 9.04%, respectively.

The Technique of Directing the Sex of Betta Fish (Betta Sp.) Larval Stage (H3, H5, and H7) using Coconut Water (Cocos Nucifera) Rohayati, Cucu; Utomo, Deny Sapto Chondro; Adiputra, Yudha Trinoegraha
Jurnal Perikanan dan Kelautan Vol. 26 No. 3 (2021): October
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Betta (Betta sp.) is an anabantidae fish with sexual development that can be directed at the embryonic and larval stages. One way to direct the sex development of these fish is by using hormone therapy using natural ingredients. Coconut water is one of the natural ingredients that can be used because it contains high potassium. This potassium can convert cholesterol in fish body tissue to pregnenolone which will affect the increase in testosterone production. This study aims to determine the best dose and age group in directing the sex of Betta fish experimentally using a randomized block design (RBD) with 4 treatments and 3 replications. The immersion treatment of larvae in the age group of 3 days, 5 days, and 7 days for 15 hours with a dose of 0%, 10%, 20%, and 30% coconut water was carried out in a 3 L jar containing 30 larvae. The results showed that coconut water was able to influence the increase in the percentage of male betta fish (P <0.05) with the highest value at a dose of 30% as much as 86.26 ± 8.34% and the 3 day larvae age group of 72.78 ± 16.03, and have a different effect on other treatments. In addition, the survival calculated after immersion shows a value of 100%, meaning that coconut water does not have a harmful effect on fish. Based on the results of this study, the best treatment for directing the sex of betta fish is a dose of 30% coconut water with the 3 day age group.

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