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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Kamaya: Jurnal Ilmu Agama Adi Husada Nursing Journal Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Jurnal Riset Kesehatan Nasional LOSARI: Jurnal Pengabdian Kepada Masyarakat MAHESA : Malahayati Health Student Journal Jurnal Pijar MIPA JRKN Jurnal Pengabdian West Science Jurnal Pengabdian Kepada Masyarakat Itekes Bali West Science Nature and Technology Jurnal Pengabdian Masyarakat Sundaram Berita Biologi Indonesian Journal of Global Health Research
Ida Ayu Manik Damayanti
Institut Teknologi Dan Kesehatan Bali
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EFEKTIVITAS EKSTRAK BUAH ANGGUR BALI (Vitis vinifera L. Var. Alphonso Lavallee) TERHADAP HISTOLOGI SEL BETA PANKREAS Mus musculus L. DENGAN HIPERGLIKEMIA Zabrina, Putu Tiara; Puspitasari, Lia; Damayanti, Ida Ayu Manik
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.5508

Abstract

Hiperglikemia dapat menyebabkan malfungsi sel beta pankreas yang memicu stress oksidatif. Bahan makanan yang berkaitan dengan suplai antioksidan mampu menjadi profilaksis yang efektif melawan stress oksidatif pada diabetes. Salah satu tanaman yang memiliki aktivitas antioksidan tinggi adalah Anggur Bali (Vitis vinifera L. Var. Alphonso Lavallee). Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes buah anggur Bali (V. vinifera Var. Alphonso Lavallee) dan histopatologi sel beta pankreas Mus musculus L. yang diinduksi aloksan. Penelitian ini dilakukan dengan metode true experimental study design laboratorium dengan rancangan post test only control grup design. Model hiperglikemia dilakukan dengan cara diinduksi aloksan, setelah mengalami peningkatan kadar gula darah diberikan perlakuan glibenklamid dan ekstrak buah anggur Bali selama 21 hari. Hasil penelitian menunjukkan bahwa ekstrak etanol 96% buah anggur Bali dengan dosis 200 mg/kgBB dan 400 mg/kgBB dapat meningkatkan jumlah sel beta pankreas (p<0,05) dan dosis 800 mg/kgBB tidak dapat meningkatkan jumlah sel beta pankreas (p>0,05). Ekstrak etanol 96% buah anggur Bali dapat meningkatkan jumlah sel beta pankreas.
POTENSI EKSTRAK KULIT ANGGUR BALI (Vitis vinifera L. Var. Alphonso Lavallee) TERHADAP BERAT BADAN: SKRINING FITOKIMIA DAN STUDI IN VIVO Damayanti, Ida Ayu Manik; Antari, Ni Wayan Sukma; Megayanti, Sri Dewi
Berita Biologi Vol 24 No 1 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.9564

Abstract

Sebanyak 51,7% penduduk Indonesia mengonsumsi makanan berlemak/berkolesterol/gorengan sebanyak 1-6 kali per minggu. Pada umumnya masyarakat mengonsumsi beberapa obat sintetik untuk mengatasi masalah berat badan dengan efek samping yang sangat mungkin terjadi. Dalam upaya mengatasi hal tersebut, penelitian mengenai potensi bahan alami sebagai agen penurun berat badan menjadi sangat relevan. Salah satu bahan alami yang menarik perhatian adalah kulit anggur Bali (Vitis vinifera L. Var. Alphonso Lavallee). Penelitian ini bertujuan untuk mengeksplorasi potensi ekstrak kulit anggur Bali dalam menurunkan berat badan melalui skrining fitokimia dan studi in vivo dengan memanfaatkan sumber daya lokal dalam pengembangan strategi penanganan penurunan berat badan yang efektif. Design penelitian yang digunakan adalah true experimental study dengan posttest only control group design. Kelompok yang digunakan yaitu kontrol negative, kontrol positif, P1 (100 mg/kgBB), P2 (250 mg/kgBB), dan P3 (500 mg/kgBB). Skrining fitokimia ekstrak kulit anggur Bali menunjukkan bahwa kulit anggur Bali mengandung senyawa flavonoid, fenol, alkaloid, tanin dan terpenoid. Penelitian ini menunjukkan bahwa ekstrak kulit anggur Bali (Vitis vinifera L. Var. Alphonso Lavallee) memiliki potensi signifikan dalam menurunkan berat badan mencit yang diberikan pakan tinggi lemak. Kelompok P3 menunjukkan total penurunan paling tinggi sebesar 2,4% dari hari ke-0 hingga hari ke-21dibandingkan P1 dan P2.  
Balinese Local Plants in Regulating Body Weight: A Review Damayanti, Ida Ayu Manik; Megayanti, Sri Dewi; Wulansari, Nadya Treesna
Jurnal Pijar Mipa Vol. 20 No. 8 (2025): Special Issue
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v20i8.10650

Abstract

Obesity and overweight have become major global health concerns. In Indonesia, one in five school-aged children is overweight or obese, with increasing rates observed in Bali. Dietary imbalance, sedentary lifestyles, and metabolic dysregulation contribute to abnormal body weight gain. In light of the limitations of synthetic drugs, plant-derived bioactive compounds have attracted attention as safer and multi-target alternatives for regulating body weight. This review aims to synthesise current scientific evidence on the potential of Balinese local plants in regulating body weight, identify their bioactive compounds and underlying mechanisms, and highlight research gaps to guide future nutraceutical development. This systematic review, conducted in accordance with the PRISMA 2020 guidelines, examined literature published between 2020 and 2025 retrieved from PubMed, ScienceDirect, and Google Scholar. The keywords Balinese plants, body weight, obesity, anti-obesity, plant extract, and bioactive compounds, eight studies met the inclusion criteria. The identified Balinese local plants, including Clitoria ternatea, Zingiber cassumunar, Phyllanthus acidus, Murraya paniculata, Hibiscus rosa-sinensis, Elaeocarpus grandiflorus, Tagetes erecta, and Lagerstroemia speciosa, demonstrated significant potential in regulating body weight. Their mechanisms included AMPK activation, inhibition of pancreatic lipase, suppression of adipogenesis, induction of thermogenesis, and modulation of antioxidants. Balinese local plants exhibit multi-pathway potential in regulating body weight through metabolic and physiological modulation.
Phytochemical screening of Balinese grape (Vitis vinifera L. var. Alphonso Lavallee) peel extract: Resources local plant-based lessons in biology Ida Ayu Manik Damayanti; Ni Wayan Sukma Antari
BIO-INOVED : Jurnal Biologi-Inovasi Pendidikan Vol 7, No 2 (2025): June 2025
Publisher : Master Program of Biology Education, Universitas Lambung Mangkurat, Banjarmasin, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/bino.v7i2.22239

Abstract

The utilization of local natural resources for contextual and practical-based learning is an innovative approach in biology education. This study aimed to conduct a phytochemical screening of Balinese grape (Vitis vinifera L. var. Alphonso Lavallee) peel extract and integrate the findings into plant-based biology lessons. A mixed-method approach with a one-group pretest–posttest design was applied. Phytochemical tests identified the presence of flavonoids, phenols, alkaloids, tannins, and terpenoids. The integration of results into laboratory-based lessons led to an increase in students’ average scores from 66.70 to 78.48, as well as improvement in laboratory skills, cultural awareness, and appreciation of local biodiversity. These findings demonstrate that Balinese grape peel extract is a valuable educational tool for enhancing understanding of secondary metabolites and promoting scientific literacy. The future impact of this research includes the potential for in vitro and in vivo studies to assess the extract’s biological activity, and the implementation of local plant-based learning models that support sustainable education and preservation of indigenous knowledge.Abstrak. Pemanfaatan sumber daya alam lokal untuk pembelajaran kontekstual berbasis praktikum merupakan pendekatan inovatif dalam pendidikan biologi. Penelitian ini bertujuan untuk melakukan skrining fitokimia kulit anggur Bali (Vitis vinifera L. var. Alphonso Lavallee) serta mengintegrasikan hasilnya ke dalam pembelajaran berbasis sumber daya tumbuhan lokal. Penelitian ini menggunakan metode campuran dengan desain pra-eksperimen one-group pretest–posttest. Skrining fitokimia dilakukan terhadap senyawa flavonoid, fenol, alkaloid, tanin, dan terpenoid melalui uji pereaksi spesifik. Hasil skrining menunjukkan adanya kelima senyawa aktif tersebut, Integrasi hasil ke dalam pembelajaran laboratorium meningkatkan nilai rerata siswa dari 66,70 menjadi 78,48, serta memperkuat keterampilan laboratorium, kesadaran budaya, dan apresiasi terhadap biodiversitas lokal. Temuan ini menunjukkan bahwa penggunaan ekstrak kulit anggur Bali dapat meningkatkan literasi sains dan pemahaman peserta didik terhadap metabolit sekunder. Dampak jangka panjang dari penelitian ini mencakup potensi pengembangan studi in vitro dan in vivo untuk mengeksplorasi aktivitas biologis ekstrak serta penerapan pembelajaran berbasis sumber daya lokal yang mendukung pendidikan berkelanjutan dan pelestarian kearifan lokal.
Activity of Zuriat Fruit Extract (Hyphaene thebaica) on The DNA Fragmentation and Spermatozoa Quality of Male Mice (Mus musculus) Given Balinese Arak Ni Wayan Sukma Antari; Ida Ayu Manik Damayanti
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.11855

Abstract

Alcohol in the body is metabolized into acetaldehyde with the assistance of the enzyme alcohol dehydrogenase (ADH). Acetaldehyde, the metabolic byproduct of alcohol, plays a role in the formation of Reactive Oxygen Species (ROS). The effects of alcohol can lead to damage and death of spermatogenic cells caused by acetaldehyde, which is produced through the breakdown of alcohol by liver cells. Increased ROS levels generated by free radicals in sperm producing tissues may cause damage to the sperm membrane, thereby disrupting its stability and function. High concentrations of ROS may also exert toxic effects on sperm quality and function. This study aimed to examine the activity of Zuriat fruit (Hyphaene thebaica) extract on sperm quality and DNA fragmentation in male mice (Mus musculus) administered Balinese Arak. This research employed an experimental study design using a post-test only control group design. The results show that Zuriat fruit (Hyphaene thebaica) extract at doses of 1 g/kg body weight and 2 g/kg body weight significantly influenced sperm count, motility, viability, morphology, and DNA fragmentation (p < 0,05).
Plant-Based Natural Resource Management Strategies Using Fruits for Cholesterol Regulation Damayanti, Ida Ayu Manik; Wulansari, Nadya Treesna; Megayanti, Sri Dewi
West Science Nature and Technology Vol. 3 No. 04 (2025): West Science Nature and Technology
Publisher : Westscience Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58812/wsnt.v3i04.2630

Abstract

Cardiovascular diseases remain a leading cause of global mortality, with hypercholesterolemia representing a major modifiable risk factor. Growing interest has emerged in plant-based strategies, particularly fruit utilization, as sustainable approaches for cholesterol regulation. This study aimed to systematically review the pharmacological mechanisms of fruit-derived secondary metabolites involved in cholesterol lowering and to explore their relevance within plant-based natural resource management. A systematic literature review was conducted following PRISMA guidelines using ScienceDirect, PubMed, and Google Scholar databases. Evidence from in vivo and in vitro studies demonstrates that various fruits including Gedong Gincu mango, sweet orange, red dragon fruit, buni fruit, Rosa roxburghii, pineapple, Synsepalum dulcificum, mulberry, and Hiyung cayenne pepper contain bioactive secondary metabolites such as flavonoids, phenolics, anthocyanins, carotenoids, triterpenoids, alkaloids, saponins, dietary fiber, and antioxidant enzymes. These compounds exert cholesterol-lowering effects through multiple pharmacological pathways, including inhibition of cholesterol synthesis and absorption, enhancement of bile acid excretion, modulation of lipid metabolism–related gene expression, and reduction of oxidative stress and inflammation. Several fruit-based interventions showed significant reductions in total cholesterol and LDL levels, with some demonstrating efficacy comparable to conventional hypolipidemic agents. These findings highlight the potential of fruit-based natural resources as functional, sustainable, and scientifically supported strategies for cholesterol regulation and cardiovascular disease prevention.
Optimalisasi Manajemen Pemberdayaan Masyarakat Berbasis Toga Dalam Upaya Pengendalian Diabetes Melitus Damayanti, Ida Ayu Manik; Antari, Ni Wayan Sukma; Dharmapatni, Ni Wayan Kesari; Wulansari, Nadya Treesna; Kusuma, Ida Bagus Separsa; Artha, I Wayan Oktapratama; Aristadewi, Ida Ayu Mas
Jurnal Abdimas ITEKES Vol 5 No 2 (2026)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Institute Teknologi dan Kesehatan (ITEKES) Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37294/jai.v5i2.857

Abstract

Diabetes melitus merupakan penyakit tidak menular dengan prevalensi yang terus meningkat dan menjadi tantangan serius dalam pembangunan kesehatan masyarakat. Kondisi ini berdampak pada kualitas hidup, serta risiko komplikasi kronis, sehingga diperlukan upaya pengendalian secara promotif dan preventif berbasis perubahan perilaku. Salah satu potensi lokal yang dapat dimanfaatkan adalah Tanaman Obat Keluarga (TOGA), namun pemanfaatannya di masyarakat masih belum optimal. Kegiatan ini bertujuan untuk meningkatkan pengetahuan dan perilaku masyarakat dalam optimalisasi manajemen pemberdayaan masyarakat berbasis toga dalam upaya pengendalian diabetes melitus. Metode yang digunakan adalah pemberdayaan masyarakat berbasis partisipatif dengan melibatkan 37 peserta di Desa Pedungan. Kegiatan meliputi pre-test, edukasi dan pelatihan (green training), pemeriksaan gula darah, pendampingan (green involvement), serta post-test dan evaluasi (green performance). Data dianalisis secara deskriptif dan inferensial menggunakan uji paired t-test serta skala Likert untuk perilaku. Hasil menunjukkan adanya peningkatan signifikan pengetahuan peserta dari nilai rata-rata 54,86 menjadi 83,00 (p < 0,05). Selain itu, perilaku masyarakat berada pada kategori baik yang menunjukkan adanya perubahan ke arah pola hidup sehat. Pendekatan edukasi, pelatihan, dan pendampingan berbasis optimalisasi manajemen efektif dalam meningkatkan pengetahuan, kesadaran, dan perilaku masyarakat dalam pengendalian diabetes melitus secara mandiri dan berkelanjutan melalui pemanfaatan TOGA.  Kata kunci : Diabetes melitus, TOGA, pemberdayaan masyarakat, perilaku kesehatan
Guided inquiry improves undergraduate students psychomotor skills in gram staining practicum Nadya Treesna Wulansari; Ni Wayan Kesari Dharmapatni; I Putu Gede Sutrisna; Anak Agung Istri Mas Padmiswari; Kadek Buja Harditya; Ida Ayu Manik Damayanti
BIO-INOVED : Jurnal Biologi-Inovasi Pendidikan Vol 8, No 2 (2026): June 2026
Publisher : Master Program of Biology Education, Universitas Lambung Mangkurat, Banjarmasin, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/bino.v8i2.25954

Abstract

Gram staining of bacteria is an important practical material in Microbiology and Parasitology courses as part of Basic Nursing Science. This technique is widely used for the initial identification of pathogenic microorganisms. However, observations indicate that some students have not mastered the Gram staining technique optimally, leading to difficulties in interpreting microscopic findings. Until now, practical learning has been dominated by demonstration and one way instruction methods, so that the development of students' psychomotor skills has not been optimal. This study aims to evaluate the effectiveness of the guided inquiry method in improving the psychomotor skills of nursing students in Gram staining practical. The study used a quasi experimental design with a pretest–posttest control group design. The research subjects were students of the Nursing Study Program, semester II of the 2025/2026 Academic Year, consisting of two classes, namely the guided inquiry class (70 students) and the control class (72 students). Psychomotor skill data were analyzed based on the difference between posttest and pretest scores. The difference in psychomotor skill improvement between the two groups was analyzed using the Independent Samples t-test at a significance level of 0.05. The results showed a significant difference in the difference in psychomotor skill scores between the guided inquiry group and the control group, with the guided inquiry group having a higher average score. This indicates that guided inquiry learning is effective in improving students' psychomotor skills in Gram staining practicums.Abstrak. Pewarnaan Gram pada bakteri merupakan materi praktikum penting dalam mata kuliah Mikrobiologi dan Parasitologi sebagai bagian dari Ilmu Keperawatan Dasar. This technique is widely used for the initial identification of pathogenic microorganisms, so mastering these psychomotor skills is essential for students to ensure the accuracy of clinical diagnoses.  Namun, pengamatan menunjukkan bahwa sebagian mahasiswa belum menguasai teknik pewarnaan Gram secara optimal, sehingga menimbulkan kesulitan dalam menafsirkan temuan mikroskopis. Hingga saat ini, pembelajaran praktikum masih didominasi oleh metode demonstrasi dan pengajaran satu arah, sehingga pengembangan keterampilan psikomotorik mahasiswa belum optimal. Penelitian ini bertujuan untuk mengevaluasi efektivitas metode inquiry terarah dalam meningkatkan keterampilan psikomotor mahasiswa keperawatan pada praktikum pewarnaan Gram. Penelitian ini menggunakan desain kuasi-eksperimental dengan desain pretest–posttest kelompok kontrol. Subjek penelitian adalah mahasiswa Program Studi Keperawatan, semester II Tahun Akademik 2025/2026, yang terdiri dari dua kelas, yaitu kelas inquiry terarah (70 mahasiswa) dan kelas kontrol (72 mahasiswa). Data keterampilan psikomotor dianalisis berdasarkan selisih skor posttest dan pretest. Perbedaan peningkatan keterampilan psikomotor antara kedua kelompok dianalisis menggunakan Uji-t Sampel Independen pada tingkat signifikansi 0,05. Hasil menunjukkan adanya perbedaan yang signifikan pada selisih skor keterampilan psikomotor antara kelompok pembelajaran inquiry terarah dan kelompok kontrol, dengan kelompok pembelajaran berbasis penyelidikan terpandu memiliki skor rata-rata yang lebih tinggi. Hal ini menunjukkan bahwa pembelajaran berbasis inquiry terarah efektif dalam meningkatkan keterampilan psikomotorik siswa pada praktikum pewarnaan Gram.
Secondary Metabolites Content and Effects of Zuriat Fruit (Hyphaene Thebacia) Brewing on the Reduction of Blood Glucose and Histopathology of β-Pancreatic Cells of Male Mice (Mus musculus) Ni Wayan Sukma Antari; Ida Ayu Manik Damayanti; Ni Luh Adi Satriani
Indonesian Journal of Global Health Research Vol. 8 No. 2 (2026): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v8i2.2419

Abstract

Diabetes mellitus is caused by genetic factors, such as having a family history of the disease, being overweight (obesity), autoimmune diseases, and abnormalities in pancreatic β-cells. This study was conducted to examine the secondary metabolite content and effects of Zuriat fruit (Hyphaene Thebacia) infusion on reducing blood glucose and histopathology of pancreatic β-cells in male mice (Mus musculus). The results were analyzed using powder weights of 2 g/kg body weight and 5 g/kg body weight. This study employed a true experimental study design in a laboratory setting, where the research was conducted rigorously, controlling all factors that could interfere with internal validity. The study design used a posttest-only control group design, where the test was conducted after treatment. The results were processed using the One-Way ANOVA test. Administering a brew of zuriat fruit (Hyphaene thebacia) with a powder weight of 2 grams and 5 grams can reduce blood glucose levels in male mice. Administration of a brew of zuriat fruit (Hyphaene thebacia) with a powder weight of 2 grams and 5 grams can increase pancreatic β-cells. Administering zuriat fruit infusion has been shown to lower blood glucose levels in male mice compared to the control group.
Co-Authors A.A. Istri Mas, Padmiswari A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Abda Abda Adiana, I Nengah Adnyana, I Kadek Budi Anak Agung Istri Mas Padmiswari Ariani, Ni Komang Sri Aristadewi, Ida Ayu Mas Artana, I Wayan Budi Artha, I Wayan Oktapratama Asthadi Mahendra Bhandesa, Asthadi Mahendra Clarisa, Gusti Ayu Della Deni Redita, I Komang Dira, Made Asmarani Dwi Arymbhi Sanjaya Gede Surya Indrawan Gede, Surya Indrawan Gusti Ayu Della Clarisa I Gusti Agung Tresna Wicaksana I Ketut Alit Adianta I Ketut Junitha I Ketut Swarjana I Made Bayu Jaya Kusuma I Putu Artawan I Putu Gde Yudara Sandra Putra I Putu Gede Sutrisna I Putu Gede Sutrisna . I Wayan Budi Artana Ida Bagus Made Suaskara Ida Bagus Separsa Kusuma Kadek Buja Harditya Kadek Buja Harditya Kadek Maya Cyntia Dewi Kadek Nuryanto Lumbantobing, Gita Vidya Natalia Maha Putra, I Nyoman Arya Mahaputra, I Nyoman Arya Mas Aristadewi, Ida Ayu Nadya Treesna Wulansari Nadya Treesna Wulansari Ni Luh Adi Satriani Ni Luh Putu Inca Buntari Agustini Ni Made Ayu Sukma Widyandari Ni Made Krisna Ayu Lestari Ni Wayan Ayu Eka Perantini Ni Wayan Desi Bintari Ni Wayan Kesari Dharmapatni Ni Wayan Sukma Antari Ni Wayan Sukma Antari Ni Wayan Sukma Antari Ni Wayan Sukma Antari, Ni Wayan Sukma Antari Nuryanto, Kadek Nyoman Eta Risnawati Padmiswari, A.A Istri Mas Padmiswari, A.A. Istri Mas Pramana, I Kadek Bagus Ari Putu Ekawati Ida Ayu Putu Indrayoni Putu Rima Sintyadewi Sanjana, I Wayan Sri Dewi Megayanti Sri Dewi Megayanti Sri Idayani Sri Idayani Strisanti, Ida Ayu Suptika Sukaningsih, Ni Wayan Sutrisna , I Putu Gede Sutrisna, Putu Gede Suweca, I Gede Tohir, Wa. Aniyda M. Wahyunadi, Ni Made Dewi Wicaksana, IGA Tresna Widiatmaja, Gusti Panji Widyas Pranata, Gst. Kade Adi Wulansari , Nadya Treesna Zabrina, Putu Tiara