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All Journal Jurnal Ilmu Pertanian Indonesia VALENSI JURNAL KIMIA SAINS DAN APLIKASI Microbiology Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Indonesia Chimica Acta ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Chemistry Indonesian Journal of Chemical Research Menara Perkebunan Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia Kumawula: Jurnal Pengabdian Kepada Masyarakat Makara Journal of Science Indonesian Journal of Community Engagement Indonesian Journal of Jamu Analit: Analytical And Environmental Chemistry Biodiversitas Journal of Biological Diversity
Wulan Tri Wahyuni
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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FABRICATION AND ANALITICAL PERFORMANCE EVALUATION OF CARBON PASTE ELECTRODE USING CYCLIC VOLTAMMETRY Wulan Tri Wahyuni; Latifah K Darusman; Desi Herliani
Analit : Analytical and Environmental Chemistry Vol. 2, No. 02 October (2017) Analit : Analytical and Environmental Chemistry
Publisher : Jurusan Kimia FMIPA Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aec.v2i2.2017.p

Abstract

Penelitian ini bertujuan mengembangkan metode pembuatan Elektrode Pasta Karbon (EPK) yang memberikan respon voltammetri yang optimum serta menguji kinerjanya menggunakan larutan K3[Fe(CN)6] secara voltammetri siklik. Parameter yang dioptimasi meliputi nisbah komposisi grafit dan parafin, pemanasan grafit, sonikasi campuran grafit dan parafin, waktu penggerusan, dan lama penyimpanan EPK sebelum digunakan. Respons terbaik diperoleh saat EPK dibuat dengan nisbah campuran grafit dan parafin sebesar 7 : 3 dan grafit yang dipanaskan terlebih dahulu pada suhu 105 C selama 2 jam. Campuran grafit dan parafin terbaik diperoleh setelah sonikasi selama 30 menit dan waktu penggerusan selama 45 menit. EPK yang disimpan 2 hari sebelum digunakan menghasilkan respons terbaik. Pengukuran K3[Fe(CN)6] pada rentang konsentrasi 0,1 – 125 mM menunjukkan respon linear dengan koefisien determinasi (R2) sebesar 0,9998 dan 0,9991, masing-masing untuk arus puncak oksidasi dan reduksi dengan simpangan baku relatif (%SBR) sebesar 2,0% pada reaksi oksidasi dan 1,07% pada reaksi reduksi. EPK yang dihasilkan menunjukkan  stabilitas yang baik pada pemakaian selama 7 hari berturut-turut dan 96 kali pengukuran
Electrochemical Sensors Based on Graphene Oxide/PEDOT:PSS Composite Decorated with Bimetallic Gold–Silver Nanoparticles for H2O2 Detection Hidayat, Rayyan Azzahra; Putra, Budi Riza; Khalil, Munawar; Wahyuni, Wulan Tri
Makara Journal of Science Vol. 29, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hydrogen peroxide (H2O2) is a biomarker that indicates oxidative stress levels in the human body; thus, rapid and accurate detection methods are necessary to diagnose various disorders. This study developed a nonenzymatic electrochemical sensor for detecting H2O2 using bimetallic gold–silver nanoparticles (Au–Ag NPs) combined with graphene oxide and poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate)-modified glassy carbon electrode. The surface properties of the modified electrodes were characterized using scanning electron microscopy–energy dispersive spectroscopy and atomic force microscopy. The electrochemical performance of the modified electrodes was evaluated using cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry. The bimetallic Au-Ag NP-modified electrodes exhibited a higher current response to H2O2 than their monometallic counterparts. The sensor demonstrated a linear response at the H2O2 concentration of 75–200 μM, with a sensitivity of 0.0407 μA μM⁻¹ and a detection limit of 25 μM. Moreover, the modified electrodes demonstrated excellent selectivity for H2O2 and high repeatability of measurements (relative standard deviation < 5%). The detection of H2O2 levels in synthetic urine samples yielded results that were not significantly different from those obtained by the spectrophotometry method, indicating the potential of the developed sensor for clinical applications.
Application of Lemongrass Oil in Chitosan as Antimicrobial During Storage of Crystal Guava Fruit Syarifah Meutiah Eka Sari; Irma Suparto; Wulan Tri Wahyuni
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.131

Abstract

Guava crystal is a fruit that has a high selling value. One of the obstacles to marketing crystal guava fruit is the fast decay process. Many factors cause fruit to rot quickly, including microbial contamination post-harvesting. This study evaluates the effectiveness of adding lemongrass oil in chitosan coatings to protect crystal guava from microbiological damage during storage. The fruit is coated with 1% chitosan and added to lemongrass oil according to the treatment. The study used a completely randomized design with three replications. The treatment tested included 0.0, 0.1, 0.3, and 0.5% lemongrass oil. The content of lemongrass oil compounds was analyzed using gas chromatography-mass spectroscopy (GC-MS). The antibacterial test was carried out using the disc method. The parameters observed were the total plate count and total fungal count of crystal guava fruit on days 0, 3, 6, 9, 12, 15, and 18 after storage. GC-MS analysis showed that the main content of lemongrass oil is two isomers of citral, i.e., neral (38.54%) and geranial (39.26%). The results showed that the total fungal and plate count increased during storage. Chitosan coating with lemongrass oil more effectively inhibits Staphylococcus aureus than Escherichia coli. Adding 0.3% and 0.5% lemongrass oil in chitosan could restrain the rate of microbes in crystal guava until the 18th day after storage. Chitosan treatment with 0,3% lemongrass oil is recommended to be the best treatment for applying on crystal guava. Keywords: citral, GC-MS, Psidium guajava L., total plate count, total fungal count
Deteksi Kurkumin dan Bisdemetoksikurkumin dengan Teknik Voltammetri Menggunakan Elektrode Boron-Doped Diamond Wulan Tri Wahyuni; Latifah Kosim Darusman; Yuris Diksy
ALCHEMY Jurnal Penelitian Kimia Vol 14, No 2 (2018): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.14.2.19576.253-266

Abstract

Pada penelitian ini metode deteksi kurkumin dan bisdemetoksikurkumin dikembangkan dengan teknik voltammetri siklik menggunakan elektrode boron-doped diamond. Voltammogram siklik kurkumin dan bisdemetoksikurkumin dalam larutan elektrolit tetrabutilamonium heksafluorofosfat (TBAPF6)-asetonitril masing-masing menunjukkan 2 puncak oksidasi, yaitu pada potensial +0,4 V dan +1,9 V vs. Ag/AgCl untuk kurkumin, serta +0,6 V dan +2,0 V vs. Ag/AgCl untuk bisdemetoksikurkumin. Hubungan linear diperoleh antara konsentrasi kurkumin maupun bisdemetoksikurkumin dengan arus puncak oksidasinya. Hubungan linear  yang diperoleh adalah I(µA) = 8619,9C (M) + 0,7285 (R2=0,9995) dan I(µA) = 14658C (M) + 16,388 (R2=0,9299) berturut-turut untuk kurkumin puncak pertama dan kedua, serta I(µA) = 7953,2C (M) + 5,3977 (R2=0,9871) dan I(µA) = 5807,7 C (M) + 7,7616 (R2=0,9981) untuk bisdemetoksikurkumin puncak pertama dan kedua. Aplikasi metode yang dikembangkan terhadap sampel buatan yang mengandung campuran kurkumin dan bisdemetoksikurkumin menghasilkan 3 puncak oksidasi pada potensial +0,3 V, +1,2 V, dan +2,0 V vs Ag/AgCl mengindikasikan terjadinya tumpang tindih antara puncak kurkumin dan BDMC serta pergeseran puncak oksidasi. Nilai presisi pengukuran dengan metode yang dikembangkan ialah 15,86 dan 15,15% untuk kurkumin, serta 9,85 dan 31,47% untuk bisdemetoksikurkumin. Sementara nilai akurasi pengukuran ialah sebesar 96,69 dan 125,36% untuk kurkumin, serta 97,95 dan 127,54% untuk bisdemetoksikurkumin masing-masing berdasarkan puncak 1 dan 2. Hasil yang diperoleh menunjukkan performa analitik metode yang dikembangkan masih perlu ditingkatkan sebelum diaplikasikan untuk analisis kuantitatif.Detection of Curcumin and Bisdemetoxycurcumin by Voltammetric Technique using a Boron-Doped Diamond Electrode.  This study develops a cyclic voltammetry method for simultaneous detection of curcuminoid by using boron-doped diamond electrode. Curcumin and bisdemethoxycurcumin (BDMC) in tetrabutylammonium hexafluorophosphate (TBAPF6)-acetonitrile as electrolyte solution showed two oxidation peaks at +0.4 V and +1.9 V for curcumin, while those for BDMC appeared at +0.6 V and +2.0 V. Reduction peaks of both standards were not observed. In the optimum conditions, this method showed linear correlation between concentration of curcumin and BDMC with it’s oxidation peak current. The equations of I(µA) = 8619.9(C) (M) + 0.7285 (R2=0,9995) and I(µA) = 14658(C) (M) + 16.388 (R2=0.9299) were obtained for curcumin, I(µA) = 7953.2(C) (M) + 5.3977 (R2=0,9871) and I(µA) = 5807.7(C) (M) + 7.7616 (R2=0.9981) for BDMC. Meanwhile, the measurement of the artificial samples containing a mixture of curcumin and BDMC showed three oxidation peaks at +0.3 V, +1.2 V, and +2.0 V. This result indicated an overlapping between curcumin and BDMC peaks and peak shifting. Precision of the developed method was expressed as percent RSD, giving value of 15.86 and 15.15% for curcumin, and 9.85 and 31.47% for BDMC. The accuracy was 96.69 and 125.36% for curcumin, meanwhile for BDMC was 97.95 and 127.54% for peak 1 and 2. The result indicated that the method required further improvement before applied for quantitative analysis.
Diversity of medicinal plants used for skincare by Bulungan tribe in North Kalimantan, Indonesia and its melanin biosynthesis inhibition KARTINA KARTINA; IRMANIDA BATUBARA; HARLINDA KUSPRADINI; IRAWAN WIJAYA KUSUMA; WULAN TRI WAHYUNI; SAAT EGRA; TOHRU MITSUNAGA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 3 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230308

Abstract

Abstract. Kartina, Batubara I, Kuspradini H, Kusuma IW, Wahyuni WT, Egra S, Matsunaga T. 2022. Diversity of medicinal plants used for skincare by Bulungan tribe in North Kalimantan, Indonesia and its melanin biosynthesis inhibition. Biodiversitas 23: 1246-1253. Indigenous people of the Bulungan tribe in North Kalimantan, Indonesia have been utilizing plants for health purposes, including for skincare such as preventing skin from being dark due to melanin production. This study aimed to evaluate the diversity of medicinal plants utilized by the Bulungan tribe for skincare and determine the melanin biosynthesis inhibition activity. Plants having medicinal uses were investigated through literature studies and interviews with the members of Bulungan tribe in Tanjung Palas Tengah Village. Melanin biosynthesis inhibition was determined by identifying the tyrosinase inhibition potential using commercial tyrosinase enzyme, while intracellular and extracellular melanin biosynthesis production activity was determined using B16 melanoma cells. The results showed at least 19 plant species that have medicinal uses, including for skincare, with some of them having the potential for melanogenesis, both in suppressing and producing melanin. We found a plant sample with melanin suppression potential on B16 melanoma cell, namely Jatropha curcas, at 6.2 µg/ml concentration, which could decrease extracellular melanin content by 18%. Tyrosinase activity with L-tyrosine showed that Cinnamomum burmannii (root) (IC50: 3.4 ?g/mL) showed an insignificant difference with kojic acid, which served as positive control by showing an inhibitory level of (IC50: 0.14 ?g/mL). Based on the data obtained, a pharmacological study is recommended to investigate melanin biosynthesis as a skin whitening agent. We believe that this study can become basic data to discover several active compounds in future studies.
The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor NURDIANI NURDIANI; DYAH ISWANTINI; NOVIK NURHIDAYAT; WULAN TRI WAHYUNI; AGUS KARTONO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 11 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241111

Abstract

Abstract. Nurdiani, Iswantini D, Nurhidayat N, Wahyuni WT, Kartono A. 2023. The analytical performance of Saccharomyces cerevisiae and Bacillus megaterium microbial consortium as recognition element in ethanol biosensor. Biodiversitas 24: 5928-5936. Alcohol, particularly ethanol, is commonly found in human food and plays a significant role in degenerative diseases and disability. Accurate measurement of alcohol in food products is essential to ensure adherence to the Muslim halal rule. However, existing alcohol biosensor that relies on a single microbe has limitations in measuring a wide range of ethanol concentrations. To address this issue, a microbial consortium is needed to expand the measurable range. Therefore, this study aimed to develop an innovative biosensor to widen the range of measured ethanol concentrations based on the microbial consortium of Saccharomyces cerevisiae YSAPMI.2 and Bacillus megaterium BSAPMI.1. The performance of the biosensor was evaluated using the cyclic voltammetry method. The results showed that the linear range, linearity, coefficient of determination, sensitivity, and response time, were 0.02-6.0%, 0.9968, 0.9936, 83.157 µA (%)-1, and 11 seconds. The LoD ??and LoQ theoretical values ??of the method obtained in the ethanol oxidation reaction were 0.060% and 0.182%, respectively. The confirmatory test for the LoD value of 0.01% yielded a positive response, while the confirmed LoQ value of 0.02% showed good precision and accuracy. The biosensor had precise %RSD values of 0.568, 1.338, and 4.632% for the high, medium, and low ethanol concentrations, respectively. The accuracy reflected as the recovery percentage was in the range of 90.27-111.07%. The biosensor was relatively specific and had no interferences with common ethanol compounds including methanol, sodium chloride, formic acid, and glucose. The stability obtained with biofilm showed a better result reaching 88% in 70 days. Based on the result, this microbial consortium biosensor could widen the range of measured ethanol concentrations and should be further developed to create a prototype for an accurate and practical analysis.
Co-Authors . TRIVADILA Abdul Rohman Achmad Fauzi Achmad Fauzi Aditya Widianto Afifah, Salmi Agus Kartono Ahmad, Shahrul Nizam Aji Hamim Wigena Aninda Naszwa Kuswanto Anindya, Weni Antonio Kautsar Apriana, Ria Apriliani Rahmat Asoka, Shadila Fira Badrunanto Badrunanto Badrunanto, Badrunanto Bambang Prajogo Eko Wardojo Budi Riza Putra Budi Riza Putra Budi Riza Putra Darusman Pitria, Latifah K Desi Herliani Dinda Iryawati Dyah Iswantini Eka Widya Nugraha Era Rahmi Erna Subroto Eti Rohaeti Fransiska Devy Junardy HARLINDA KUSPRADINI Hera Nirwati Herdiyanto Herdiyanto Herdiyanto Herdiyanto Herliani, Desi Hidayat, Rayyan Azzahra Ika Kartika IRAWAN WIJAYA KUSUMA Irma Herawati Suparto Irma Suparto Irmanida Batubara Karomah, Alfi Hudatul KARTINA KARTINA Kilala Tilaar Kuswanto, Aninda Naszwa latifah K Darusman Latifah K Darusman Latifah K Darusman Latifah K Darusman Pitria Latifah K. Darusman LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman Latifah Kosim Darusman Latifah Kosim Darusman Luthfiana, Widya M. Rafi Mahat, Mohd Muzamir Maily Mustofa Makmum Syafi’i Manggar Arum Aristri Martha, Ina Moh Arif Rohman Nur Hakim Moh Nur Hakim Mohamad Rafi Muhammad Bachri Amran Muhammad Farid Munawar Khalil Munawaroh, Fauziyatul Nevianti Zamany Nisa, Ulfiatun NOVIK NURHIDAYAT Novik Nurhidayat Nur Zamilah Nurdiani Nurdiani NURDIANI NURDIANI Nursifa Mawadah Rahmat, Apriliani Ramadhanti, Desi Rany Zalvianita Reni Febriani Reni Febriani, Reni Ria Apriana Ridhova, Aga Rika Indri Astuti Rizna Azrianiningsari Rudi Heryanto SAAT EGRA Saat Egra Salmi Afifah Selvitia Ulandari Septaningsih, Dewi Anggraini Setyanto Tri Wahyudi Silvia, Riva Silviani, Dea Siti Aisyah SITI SA’DIAH Sri Purwanti Sri Purwanti Sri Purwanti Sukendi, Yeli Syafi’i, Makmum Syarifah Meutiah Eka Sari Syifa Muflihah Tamida Elinda Thaha, Yudi Nugraha Theresia Yolanda Avelina Tintin Sarianti TOHRU MITSUNAGA Ulfiatun Nisa Ulfiatun Nisa Utami Dyah Syafitri Waras Nurcholis Widianto, Aditya Wulansari, Laela Yessie Widya Sari Yogo Suro Priyadi Yuris Diksy Yusuf Ryadi Zamilah, Nur