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All Journal Prosiding Seminar Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) AGRIVITA, Journal of Agricultural Science Indonesian Journal of Pharmaceutical Science and Technology Indonesian Journal of Biotechnology Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Universa Medicina Jurnal Biodjati Majalah Farmaseutik OLDI (Oseanologi dan Limnologi di Indonesia) JSFK (Jurnal Sains Farmasi & Klinis) JFIOnline Jurnal Insan Farmasi Indonesia Jurnal Kefarmasian Indonesia
Subagus Wahyuono
Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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Dominant Understorey Plants Producing Herbal Medicine Materials on Homegarden Agroforestry System in Menoreh Hills, Kulon Progo District Widyastuti, S.M.; Hadi, Etik Erna Wati; Wahyuono, Subagus
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.657

Abstract

Priority products in homegarden agroforestry systems are still dominated by wood. Understorey is an important component of the homegarden community, but not a priority in its management because there is not much information about its benefits. Undestorey has several benefits such as food sources, food crops and medicinal materials. Research aimed to know the diversities of understorey species, the dominant of understorey species and the content of compounds in dominant understorey in the homegarden agroforestry system in Menoreh hill, Kulon Progo Regency. The study sites were categorized into 3 zones based on elevation, i.e. zone 1 (< 300 m above sea level (asl)), zone 2 (301-600 m asl) and zone 3 (> 600 m asl). This research method used inventory and identification of understorey species, and identification the content of dominant understorey compound by TLC method. The results showed that there were 41 species of understorey, the dominant species in zones 1, 2 and 3 were Curcuma mangga Val. Zone 3 produced the highest diversity. Based on the similarity index, there was a difference between the homegarden groups in zones 1, 2 and 3. Phenols and terpenoids were identified as in C.mangga.
UJI AKTIVITAS ANTIBAKTERI ISOLAT ACTINOMYCETES DARI RIZOSFER PADI (ORYZA SATIVA) TERHADAP SALMONELLA TYPHOSA DAN STAPHYLOCOCCUS AUREUS Ambarwati, Ambarwati; S, Tanti Azizah; Sembiring, Langkah; Wahyuono, Subagus
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.06 KB)

Abstract

Actinomycetes merupakan anggota bakteri yang dipromosikan sebagai penghasil zat  antimikrobial  terbesar.  Tujuan penelitian ini adalah untuk mengisolasi dan mengidentifikasi Actinomycetes dari rizosfer padi (Oryza sativa) yang berpotensi sebagai penghasil antibakteri dan dapat menghambat pertumbuhan Salmonella typhosa dan Staphylococcus aureus. Penelitian ini menghasilkan 17 isolat Actinomycetes, 8 isolat diantaranya mampu menghasilkan zat antibakteri. Diantara 8 isolat tersebut, satu isolat mampu menghambat pertumbuhan kedua bakteri uji, yaitu NRPR 13 yang menghambat Salmonella typhosa (dengan diameter daerah hambatan 14 mm) dan Staphylococcus aureus (12 mm). Tiga isolat mampu menghambat pertumbuhan bakteri Salmonella  typhosa, yaitu NRPR  14  (11 mm), NRPR  62 dan NRPR 67 (masing-masing 10 mm). Dan empat isolat mampu menghambat pertumbuhan Staphylococcus aureus, yaitu RPR 15 (14 mm),  NRPR 11 (17 mm), NRPR 33 (11 mm) dan NRPR 46 (16 mm).  Berdasarkan hasil penelitian ini dapat disimpulkan bahwa Actinomycetes yang diisolasi dari rizosfer padi berpotensi menghasilkan zat antibakteri. Kata kunci : Actinomycetes,  Zat Antibakteri, S. typhosa,  S. aureus.
Skrining Senyawa Antiinfeksi dari Spons yang Dikoleksi dari Bunaken, Manado Astuti, Puji; Alam, Gemini; Pratiwi, Sylvia; Hertiani, Triana; Wahyuono, Subagus
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2003): June 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.243 KB) | DOI: 10.24002/biota.v8i2.2883

Abstract

The emergence of drug resistant and multidrug-resistant microbes as well as the lack of any current chemotherapy augmented the necessity to search for new and better anti-infective drug.. This study was aim to screen potential antiinfective extracts of sponges collected from Bunaken, Manado and to report on their antibacterial and antifungal properties. Testing for antiinfective agents was conducted using dilution method. Nutrient Agar was used as the testing media and nutrient broth for the inoculation of microorganisms. Staphylococcus aureus, Escherichia coli and Salmonella typhi were used as the testing bacteria and Candida albicans as the testing fungi. Chloramphenicol was used as positive control for anti bacterial activity and Ketokonazol for antifungi. The two acetone extracts tested (MD-01aceton and MD-02aceton), all of them showed inhibition activities. Following partition with chloroform and methanol, all chloroform extracts inhibited the growth of both bacteria and fungi. These extracts then are considered to be potential candidates for further isolation and characterization as antiinfective agents.
Aktivitas Antimikroba Ekstrak Etil Asetat dari Fungi Endofit Schizophyllum commune Diisolasi dari Tanaman Bangun-bangun (Coleus amboinicus Lour.) Romadhonsyah, Fitra; Gemantari, Baiq Maylinda; Nurrochmad, Arief; Wahyuono, Subagus; Astuti, Puji
Indonesian Journal of Pharmaceutical Science and Technology 2024: Suppl. 6, No. 2 (Universitas Halu Uleo Conference)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v6i2.52598

Abstract

Coleus amboinicus Lour., yang dikenal sebagai tanaman bangun-bangun, merupakan tanaman obat yang memiliki aktivitas sebagai antimikroba. Beberapa studi melaporkan bahwa fungi endofit yang ditemukan dalam beberapa bagian jaringan tanaman mampu memproduksi senyawa aktif yang serupa dengan tanaman inangnya. Schizophyllum commune merupakan fungi endofit yang berasal dari daun C. amboinicus. Penelitian ini bertujuan untuk menguji apakah ekstrak etil asetat dari fungi endofit S. commune mempunyai aktivitas sebagai antimikroba. Produksi metabolit sekunder dari S. commune dilakukan dengan metode fermentasi menggunakan Potato Dextrose Broth (PDB). Pengujian aktivitas antimikroba dilakukan dengan metode mikrodilusi menggunakan mikroba uji yaitu Bacillus subtilis, Pseudomonas aeruginosa, Staphyloccus aureus, serta Candida albicans. Nilai persen viabilitas sel mikroba dan nilai IC50 dianalisis untuk mengkarakterisasi aktivitas antimikroba dari ekstrak etil asetat. KLT bioautografi dilakukan untuk mendeteksi potensi kandungan senyawa dalam ekstrak yang mampu menghambat pertumbuhan mikroba. Ekstrak etil asetat fungi endofit S. commune menunjukkan aktivitas antimikroba yang rendah. Nilai IC50 terbaik ditemukan terhadap B. subtilis dan C. albicans (239,23 ± 20,86 dan 143,05 ± 37,03 μg/mL). KLT bioautografi menunjukkan bahwa terdapat spot penghambatan pada B. subtilis dan C. albicans. Analisis senyawa aktif dengan pereaksi semprot anisaldehid-asam sulfat menunjukkan bahwa ekstrak mengandung senyawa terpenoid. Ekstrak etil asetat dari fungi endofit S. commune memiliki potensi sebagai antimikroba.
Optimasi Penggunaan High Shear Mixer pada Pembuatan Fraksi Alkaloid dari Daun Awar-awar (Ficus septica) dengan Desain Faktorial Kusnanto, Chelvin Ari; Gani, Andayana Puspitasari; Wahyuono, Subagus; Fakhrudin, Nanang
Jurnal Kefarmasian Indonesia VOLUME 11, NOMOR 2, AGUSTUS 2021
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v11i2.4874

Abstract

Awar-awar (Ficus septica) is an Indonesian anticancer plant that contains alkaloids as the active compound. The n-hexane insoluble fraction (FTLH) is alkaloid-containing fraction obtained from the ethanolic extract of F. septica leaves (EEDFS) by fractionation using n-hexane. High shear mixer (HSM) can be used to optimize the separation processes, including fractionation. The application of HSM in the fractionation of EEDFS is affected by fractionation duration, amount of solvent per gram extract, and strirring strength. Thus, these parameters must be optimized to obtain the optimum condition for the production of FTLH with the highest alkaloid content. This study aimed to optimize the production of FTLH using HSM with factorial designs. The single factor experimental design was employed to determine the influence of the individual variable on the alkaloid content and to define the optimum range value of each variable. A full factorial design was used to determine the presence of interaction among the factors and to determine the optimal fractionation condition. The results showed that all investigated factors independently affected alkaloid contents. The solvent volume of 14-18 mL per gram extract, the fractionation duration of 2.5-5.0 minutes, and the stirring strength of 3000 rpm are the optimal range value of each factor. The optimal conditions were solvent volume of 14.0-14.4 mL/gram extract, fractionation duration of 2.5-2.7 minutes, and strirring strength at 3000 rpm. At the optimal condition, the total alkaloid content in the FTLH reached 0.1466% or 1.3 times higher than the extract (0.1128%).
Antioxidant Activity and Pancreatic Lipase Inhibition of Curcuma aeruginosa Roxb Rhizome Fractions Suharsanti, Ririn; Wahyuono, Subagus; Yuniarti, Nunung; Astuti, Puji
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33900

Abstract

Antiobesity with a lipase inhibitor mechanism will block the hydrolysis of triglycerides into fatty acids and glycerol, while antioxidant compounds are very useful in conditions of obesity to prevent excess damage from degenerative diseases. Curcuma aeruginosa Roxb extract has been proven to have the effect of inhibiting pancreatic lipase so further effects will be seen at the fraction level. The purpose of this research is to investigate the phytochemical components ,antioxidant activity and pancreatic lipase inhibition of Curcuma aeruginosa Roxb fractions. Ethanolic extract of the Curcuma aeruginosa Roxb rhizome was separated using the solid-liquid chromatography with 3 different solvents (n-hexane, ethyl acetate, ethanol) to give n-hexane (HF), ethyl acetate (EAF), and ethanol (EF), and the insoluble (IF) fractions. Each fraction detected phenolics, flavonoids, alkaloids, tannins, saponins, triterpenoids/steroids. EF has the highest total flavonoid and phenolic content. Antioxidant activity of all fractions were measured using DPPH reduction, ABTS, and FRAP methods. The best antioxidant activity of all fractions using the DPPH method was EF with IC50 21.93 ± 3.39µg/mL, ABTS method was HF with IC50 24.56±1,03 µg/mL and FRAP method was IF with IC50 20.79±1,03 µg/mL. Totals of phenolics and flavonoids in EF strongly support the antioxidant activity of the DPPH method. The highest inhibition of pancreatic lipase was found in EAF at 35.16±0.24 % (100 µg/ml). There was significant difference between EAF and xenical (orlistat) (p
FORMULASI DAN EVALUASI FISIK SEDIAAN TABLET NANOPARTIKEL EKSTRAK DAUN DADANGKAK (Hydrolea spinosa L.) Niah, Rakhmadhan; Wahyuono, Subagus; Prihandiwati, Erna
Jurnal Insan Farmasi Indonesia Vol 7 No 3 (2024): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jdkpqw89

Abstract

The utilization of traditional medicines offers a safer alternative for treatment, particularly regarding side effects and toxicity. One herbal plant known for its antioxidant activity is dadangkak leaves (Hydrolea spinosa L.), containing flavonoids, polyphenols, and tannins. Ethanolic extracts of dadangkak leaves with antioxidant potential need development into tablet dosage forms. This study aims to determine the optimal formula for nanoparticle tablets containing dadangkak leaf extract. The extract was obtained via maceration using 96% technical ethanol, followed by evaporation to produce a thick extract. Nanoparticles were prepared by mixing the extract with 96% technical ethanol, distilled water, a chitosan solution in acetic acid, and a NaTTP solution. Particle size was analyzed using a Particle Size Analyzer (PSA). Tablet formulations involved varying concentrations of Na alginate and Avicel PH 102, using direct compression. Results showed that Na alginate and Avicel PH 102 concentrations affected tablet physical properties. Avicel PH 102 increased hardness and disintegration time, while Na alginate increased friability. Based on evaluations, formula 2 was determined to be the best.
Jamur Endofit Arthrinium sp., Sumber Potensial Senyawa Obat Review Eltivitasari, Andita; Wahyuono, Subagus; Astuti, Puji
JSFK (Jurnal Sains Farmasi & Klinis) Vol 8 No 3 (2021): J Sains Farm Klin 8(3), Desember 2021
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.8.3.228-241.2021

Abstract

Keberadaan jamur endofit di alam sangat melimpah dan mudah ditemukan pada jaringan tanaman antara lain pada akar, batang, daun, bunga atau biji. Endofit berkoloni di area antar dan intra-sel dari jaringan tanaman tanpa efek merugikan pada tanaman tersebut. Jamur endofit mempunyai kemampuan memproduksi senyawa bioaktif yang dapat sama atau berbeda dari tumbuhan inangnya dan mempunyai efek farmakologis.  Arthrinium sp. merupakan contoh jenis jamur endofit yang keberadaannya melimpah. Arthrinium sp. diketahui mempunyai kandungan metabolit sekunder yang potensial untuk dikembangkan. Senyawa golongan terpenoid, alkaloid, fenolik dan beberapa golongan lain ditemukan dalam jamur endofit Arthrinium sp. Senyawa yang dihasilkan oleh Arthrinium sp. dilaporkan menunjukkan aktivitas biologis yang beragam yaitu sebagai antimikroba, antioksidan, antikanker dan aktivitas yang lainnya. Tulisan ini merangkum pengetahuan, studi dan laporan mengenai jamur endofit Arthrinium sp., kajian aktivitas biologi serta metabolit sekunder yang dihasilkan yang berpotensi dikembangkan sebagai penemuan obat baru yang berasal dari alam.
AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BERBAGAI JENIS SAYURAN SERTA PENENTUAN KANDUNGAN FENOLIK DAN FLAVONOID TOTALNYA Nurhaeni, Farisya; Lestari, Tri; Wahyuono, Subagus; Rohman, Abdul
Media Farmasi: Jurnal Ilmu Farmasi Vol. 11 No. 2: September 2014
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v11i2.1876

Abstract

Tumbuh-tumbuhan diketahui kaya antioksidan alami seperti vitamin E, vitamin C, beta karoten dan flavonoid.Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan10 jenis sayuran, Amaranthus lividus L.sensu Thell., Cichorium endivia, Brassica juncea (L.) Czern., Sauropus androgynus (L.) Merr., Ocimum bassilicum, Brassica oleracea var.botrytis  L.,  Brassica oleracea var.botrytis L. subvar cauliflora DC., Brassica oleracea var.capitata L., Brassica oleracea var.capitata L. forma rubra, Cosmos caudatus H.B.K dan untuk mengetahui seberapa besar kontribusi senyawa fenolik dan flavonoid pada aktivitas antioksidannya. Uji aktivitas antioksidan dilakukandengan metode DPPH (2,2-difenil-1-pikril hidrazil) dengan menggunakan baku pembanding kuersetin. Analisis kandungan fenolik total ditentukan oleh kemampuan sampel untuk mereduksi reagen Folin Ciocalteau. Analisis flavonoid total dilakukan secara spektrofotometri didasarkan adanya kemampuan flavonoid  membentuk kompleks yang berwarna kuning dengan AlCl3 , yang selanjutnya akan bereaksi dengan  NaOH membentuk warna merah muda yang dapat diukur absorbansinya pada panjang gelombang 510 nm. Hasil penelitian menunjukkan ekstrak kenikir (Cosmos caudatus H.B.K.) memiliki aktivitas antioksidan yang paling tinggi dengan nilai IC50 19,49 µg/ml. Hubungan antara aktivitas antioksidan 10 jenis sayuran dengan kandungan fenolik total diperoleh persamaan regresi linear  y = -34,94 x +562,5, r2 = 0,235 , sementara itu hubungan aktivitas antioksidan dengan flavonoid totalnya diperoleh persamaan y = -16,57 + 755,9, r2 = 0,471. 
Co-Authors . HERNAYANTI Abdul Rohman Aditya Krishar Karim Ag. Yuswanto Ahmad Hamim Sadewa Ali Djamhuri Ambarwati Ari Sartinah, Ari Ariani Hatmanti Arief Nurrochmad Baiq Maylinda Gemantari Bambang Hariono Djoko Santoso Eltivitasari, Andita Endang Lukitaningsih Erna Prawita Setyowati Farisya Nurhaeni Galih G, Galih Gani, Andayana Puspitasari Gemini Alam Hadi, Etik Erna Wati Ibnu G Gandjar, Ibnu G Ibnu G. Ganjar, Ibnu G. Ida Ayu Putu Sri Widnyani Imono Argo Donatus, Imono Argo Isnindar Isnindar JAKA WIDADA Kumiko Terada Kusnanto, Chelvin Ari KV Rao, KV L. Hartanto Nugroho LANGKAH SEMBIRING Linus Yhani Chrystomo Mae Sri Hartati Wahyuningsih Marchaban Marchaban, Marchaban Marchaban, . Marchaban, . Mark T Hamann, Mark T Mimiek Murrukmihadi Moch. Saiful Bachri Nanang Fakhrudin, Nanang Niah, Rakhmadhan Nunung Yuniarti Peni Susilowati Putri, Peni Susilowati Pratiwi, Sylvia Prihandiwati, Erna Puji Astuti PUSPITA LISDIYANTI Ririn Suharsanti, Ririn Romadhonsyah, Fitra S.M. Widyastuti Samsul Hadi Sofia Mubarika Haryana Sri Sudewi, Sri Sri Wahdaningsih Sudibyo Martono Sugeng Riyanto Sukarti Moeljopawiro Sutomo Sutomo Tanti Azizah S Tatsuo Takeya, Tatsuo Tri Lestari Tri R Nuringtyas, Tri R Triana Hertiani Tsutomu Nohno Widyastuti, SM